Abstract:
:As a preliminary investigation to establish a cryopreservation method suited for bioartificial livers (BALs), three-dimensional (3-D) cryopreservation experiments with fibroblasts were performed, in which the cells were firstly seeded into a porous scaffold, and the scaffold containing the cells was then cryopreserved. After thawing, 65% of the initially applied cells were still attached to the scaffold, and this efficiency was significantly higher than that in the control experiments (39%), in which fibroblasts cryopreserved in a suspension were seeded into the scaffold. This higher efficiency was mainly caused by higher immobilization efficiency at the time of cell seeding (83%) than in the controls (54%). Collagen coating of the scaffold in the 3-D cryopreservation enhanced immobilization efficiency at the time of cell seeding, and 1-day precultures before the 3-D cryopreservation considerably improved cell growth after thawing. From these favorable results, this 3-D cryopreservation method may become useful for developing BALs.
journal_name
Artif Organsjournal_title
Artificial organsauthors
Miyoshi H,Ehashi T,Ohshima N,Jagawa Adoi
10.1111/j.1525-1594.2009.00933.xsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
609-14issue
7eissn
0160-564Xissn
1525-1594pii
AOR933journal_volume
34pub_type
杂志文章abstract::The objective of this study was to evaluate the hemodynamic performance and gaseous microemboli (GME) handling ability of a simulated neonatal extracorporeal life support (ECLS) circuit with an in-line continuous renal replacement therapy (CRRT) device. The circuit consisted of a Maquet RotaFlow centrifugal pump or HL...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12987
更新日期:2018-04-01 00:00:00
abstract::This article reviews the clinical, biological, radiological, and pathological procedures and their respective indications for the practical diagnosis of the following various histological patterns of renal osteodystrophy: osteitis fibrosa due to parathyroid hormone (PTH) hypersecretion: osteomalacia or rickets due to ...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1046/j.1525-1594.1998.06198.x
更新日期:1998-07-01 00:00:00
abstract::Small-caliber decellularized xenografts with surface heparin coating are known to reduce in vivo thrombogenicity. This study was performed to examine whether heparin coating on the small-caliber decellularized xenografts would reduce macrophage infiltration and intimal hyperplasia. In a rabbit model of bilateral carot...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00748.x
更新日期:2009-06-01 00:00:00
abstract::Dual chamber (DC) peritoneal dialysis (PD) dialysates contain fewer glucose degradation products (GDPs), so potentially reducing advanced glycosylation end products (AGEs), which have been reported to increase inflammation and cardiovascular risk. We wished to determine whether use of DC dialysates resulted in demonst...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13768
更新日期:2020-07-09 00:00:00
abstract::Following implantation of a left ventricular assist device (LVAD), acceptable functional performance is now being achieved; however, peak VO2 and peak work load (watts) remain considerably limited. Maximum physical capacity is essentially dependent on generated cardiac output (CO) and arteriovenous oxygen difference (...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13102
更新日期:2018-07-01 00:00:00
abstract::Interest in the effects and possible mechanisms of hemodialysis on schizophrenic patients has led to a double-blind evaluation of the effects of plasmapheresis on symptoms of schizophrenia. Also, plasmapheresis was felt to be an invaluable tool for investigating the autoimmune theory of schizophrenia. Patients (n = 10...
journal_title:Artificial organs
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1525-1594.1983.tb04203.x
更新日期:1983-08-01 00:00:00
abstract::Twelve patients, 8 male and 4 female, aged from 26 to 70 years were studied. They were on hemodialysis (HD) 4 h three times weekly with cuprophan hollow fiber dialyzers (Gambro 120M) and dialysate containing 35 mEq/L of acetate. The study compares two random HD sessions on each patient. Sixty minutes prior to one rand...
journal_title:Artificial organs
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1525-1594.1989.tb01551.x
更新日期:1989-10-01 00:00:00
abstract::Hypertension is an important primary etiology of end-stage renal disease as well as a major factor responsible for progression of renal disease due to other causes. Multiple genetic and environmental factors are responsible for the variable prevalence of hypertension in various parts of the world. Although the frequen...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2002.07067.x
更新日期:2002-09-01 00:00:00
abstract::The aim of this meta-analysis was to determine whether pulsatile perfusion during cardiac surgery has a lesser effect on renal dysfunction than nonpulsatile perfusion after cardiac surgery in randomized controlled trials. MEDLINE, EMBASE, and the Cochrane Central Register of Controlled Trials were used to identify ava...
journal_title:Artificial organs
pub_type: 杂志文章,meta分析
doi:10.1111/aor.12452
更新日期:2015-09-01 00:00:00
abstract::A new intravascular pumping oxygenator (IVPO) was developed for intravascular gas exchange and circulatory assistance in critically ill patients with respiratory and circulatory failure. The IVPO utilizes new silicone hollow fibers (diameter, 1 mm; membrane width, 50 microns) and consists of two driving tubes for the ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb00703.x
更新日期:1997-01-01 00:00:00
abstract::Cardiovascular disorders are the leading causes of mortality and morbidity in the developed world. Cell-based modalities have received considerable scientific attention over the last decade for their potential use in this clinical arena. This review was intended as a brief overview on the subject of therapeutic potent...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1111/j.1525-1594.2006.00209.x
更新日期:2006-04-01 00:00:00
abstract::Existing peritoneal dialysis (PD) solutions were formulated for the maintenance of fluid and electrolyte balance, correction of metabolic acidosis, and the removal of metabolic waste products. Solutions currently in development are designed to improve biocompatibility by neutralizing pH and reducing glucose degradatio...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1046/j.1525-1594.1998.06022.x
更新日期:1998-01-01 00:00:00
abstract::Wall shear stress on the pump casing cover was measured using a surface-mounted hot-film sensor. In addition, the shear stress distribution in the pump was qualitatively investigated by means of oil-film visualization. The characteristics of shear stress in the pump are discussed, including the results on the oil-film...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2007.00421.x
更新日期:2007-07-01 00:00:00
abstract::This article presents results from the field calculation in a model of biological tissue taking into account the electroporation process. The electroporation model used in this study was proposed and experimentally verified by Glaser et al. for planar membranes. The numerical method for field calculation is based on a...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2005.29085.x
更新日期:2005-06-01 00:00:00
abstract::The aim of the present study was to compare the effects of ProTaper Gold, WaveOne Gold, ProTaper Universal, and WaveOne instruments on the amount of apically extruded debris. Eighty mandibular premolar teeth with straight root canals were selected and assigned to four groups (n = 20). The root canals were instrumented...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12675
更新日期:2016-08-01 00:00:00
abstract::The blood flow through the hemofilter device is the key point for ultrafiltrate production during continuous arteriovenous hemofiltration. It mainly depends on arteriovenous pressure gradient and vascular access, which is the major problem for arteriovenous hemofiltration in infants and small children. In infants, we ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1988.tb01518.x
更新日期:1988-02-01 00:00:00
abstract::A physiological controller is a key component to optimize the performance of left ventricular assist devices (LVADs) from short term to long term (10-20 years). The difficulties of its design lie in the inaccurate modeling of any particular human cardiovascular system because of limited knowledge, the large fluctuatio...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2004.07381.x
更新日期:2004-10-01 00:00:00
abstract::Cardiac surgery with cardiopulmonary bypass is associated with a systemic inflammatory response syndrome. The major clinical features of this include a reduction of pulmonary compliance and increased extracellular fluids, with increased pulmonary shunt fraction similar to acute respiratory distress syndrome, thus resu...
journal_title:Artificial organs
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1525-1594.2009.00777.x
更新日期:2009-08-01 00:00:00
abstract::Continuous hemodiafiltration (CHDF) has been performed for the treatment of severe acute pancreatitis. Phospholipase A2 (PLA2) is one of the important mediators which exacerbate acute pancreatitis, but whether PLA2 can be removed by CHDF is unclear. In this study, the kinetics of group IB and group IIA PLA2 was examin...
journal_title:Artificial organs
pub_type: 临床试验,杂志文章
doi:10.1111/j.1525-1594.2007.00398.x
更新日期:2007-05-01 00:00:00
abstract::The quality control of bicarbonate hemodialysis requires the determination of TCO2, either in blood or in dialysate. The use of the M + D TCO2 kit, a bedside micromethod, gave the authors an economical and reliable solution to this problem. ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1985.tb04404.x
更新日期:1985-11-01 00:00:00
abstract::Activated clotting time (ACT) is the most commonly used laboratory test to control the heparin effect during extracorporeal techniques. The study was undertaken in order to test in vitro the influence of heparin, platelet count, hematocrit, platelet fragmentation, and platelet activation on ACT. Blood was drawn from v...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2000.06552.x
更新日期:2000-07-01 00:00:00
abstract::The vibrating flow pump (VFP) can generate high frequency oscillated blood flow. Because of the high frequency driving with short stroke volume, the pump system can be small. The disposable type VFP (D-VFP) was developed for use for extracorporeal circulation. The electromagnetic actuator was detached from the vibrati...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb03724.x
更新日期:1997-07-01 00:00:00
abstract::In patients undergoing exchange-transfusion with hemoglobin (Hb)-based oxygen (O₂) carriers (HBOC), native Hb coexists with newly transfused Hb. The two Hb types share the same arterial and venous PO₂, but their affinities for O₂ vary. A simple spreadsheet model is described aiming at evaluating the contribution of ea...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2011.01296.x
更新日期:2012-02-01 00:00:00
abstract::Renal Solutions Allient Sorbent Hemodialysis System utilizes a two-chambered pneumatic pump (Pulsar Blood Pump, Renal Solutions, Inc., Warrendale, PA, USA) to avoid limitations associated with peristaltic pumping systems. Single-needle access is enabled by counter-pulsing the two pump chambers, thereby obviating compl...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00724.x
更新日期:2009-04-01 00:00:00
abstract::The products of complement activation, including C3a, C4a, C5a, and their des Arg derivatives, have been used by many investigators as indicators of blood or foreign surface bioincompatibility, e.g., during hemodialysis. One problem encountered is that after extracorporeal circulation over some dialysis membranes very...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1986.tb02518.x
更新日期:1986-02-01 00:00:00
abstract::The effect of blood hematocrit (HCT) on a noninvasive flow estimation algorithm was examined in a centrifugal implantable rotary blood pump (iRBP) used for ventricular assistance. An average flow estimator, based on three parameters, input electrical power, pump speed, and HCT, was developed. Data were collected in a ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2007.00339.x
更新日期:2007-01-01 00:00:00
abstract::This work describes the preparation and characterization of anionic collagen composites with rhamsan and vinylidene fluoride-trifluorethylene with improved rheological and dielectric properties without loss of collagen secondary structure with an interaction occurring between both macromolecules of the composites. On ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.1998.06109.x
更新日期:1998-03-01 00:00:00
abstract::The postdialysis levels of serum sodium concentration, urea concentration, and osmolality, as well as the magnitude of both transcellular fluid shifts and sodium removal, were predicted based on computer modeling without measuring body fluid volumes. A 4-h hemodialysis was performed in five patients at a constant ultr...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1983.tb04219.x
更新日期:1983-11-01 00:00:00
abstract::Extracorporeal membrane oxygenation (ECMO) has long been the sole means of mechanical support for pediatric patients with end-stage cardiac failure, but has a high waitlist mortality and a reported survival to hospital discharge of less than 50%. The purpose of this study was to compare waitlist mortality and survival...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00969.x
更新日期:2010-12-01 00:00:00
abstract::Thousands of ventricular assist devices (VADs) currently provide circulatory support to patients worldwide, and dozens of heart pump designs for adults and pediatric patients are under various stages of development in preparation for translation to clinical use. The successful bench-to-bedside development of a VAD inv...
journal_title:Artificial organs
pub_type: 杂志文章,评审
doi:10.1111/aor.12600
更新日期:2016-06-01 00:00:00