Confocal microscopy evidence of prion protein fragment hPrP[173-195] internalization in rat B104 neuroblastoma cell line.

abstract：:The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-s...

journal_title：Protein and peptide letters

authors： Staiano M,Strianese M,Varriale A,Di Giovanni S,di Mase DS,Dell'Angelo V,Ruggiero G,Labella T,Pellecchia C,D'Auria S

更新日期：2012-06-01 00:00:00

abstract：:BmK ITa1 cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni(2+)-IDA-sepharose 6B column was toxic for insect, which indicated t...

journal_title：Protein and peptide letters

authors： Liu Z,Yang GZ,Chi CW,Wu XF

更新日期：2002-10-01 00:00:00

abstract：:Endonuclease tRNase Z catalyzes the generation of the mature 3' end of tRNA precursors through specific endonucleolytic cleavage. The enzyme has been characterized from organisms representative of all domains of life as well as from organelles, and the crystal structure of three bacterial enzymes has been determined. ...

journal_title：Protein and peptide letters

authors： Ceballos M,Vioque A

更新日期：2007-01-01 00:00:00

abstract：:We investigated the in vitro antibiotic activity of the 19-amino acid antimicrobial peptide HP (2-20), derived from the N-terminus of Helicobacter pylori Ribosomal Protein L1 (RPL1), against antibiotic susceptible and resistant pathogens from a patient with gallstones. HP (2-20) was active against antibiotic-susceptib...

journal_title：Protein and peptide letters

authors： Park Y,Hahm JS,Hahm KS

更新日期：2006-01-01 00:00:00

abstract：:G-protein betagamma dimers are prime regulators transmitting extracellular signals to wide-ranging cellular effectors including phosphoinositide 3-kinase (PI3K) isoforms beta and gamma. Recombinant Gbetagamma purified from Sf9 cells via metal-affinity and anion exchange chromatography exhibited a wortmannin-insensitiv...

journal_title：Protein and peptide letters

authors： Shymanets A,Ahmadian MR,Nürnberg B

更新日期：2009-01-01 00:00:00

abstract：:We have studied the effect of guanidinium chloride (Gdn.Cl) and different osmolytes such as betaine, trimethylamine-N-oxide (TMAO) and urea on the rate of chymotrypsin catalyzed reaction. The rates were measured using three synthetic chromogenic substrates, succinyl-ala-ala-pro-arg-pNA (AAPR), succinyl-ala-ala-pro-leu...

journal_title：Protein and peptide letters

authors： Garringer M,Qasim MA

更新日期：2016-01-01 00:00:00

abstract：:The prediction of protein function is a difficult and important problem in computational biology. In this study, an efficient method is presented to predict protein function with sequence composition information. Four kinds of basic building blocks of protein sequences are investigated, including N-grams, binary profi...

journal_title：Protein and peptide letters

authors： Dong Q,Zhou S,Deng L,Guan J

更新日期：2010-06-01 00:00:00

abstract：:Glycation, a non-enzymatic reaction between glucose and protein is the primary cause of diabetic complications. Albumin, the most abundant plasma protein undergoes glycation both in vivo and in vitro. The influence of albumin on glycation of less abundant proteins has not been addressed. For the first time, we show th...

journal_title：Protein and peptide letters

authors： Bhonsle HS,Singh SK,Srivastava G,Boppana R,Kulkarni MJ

更新日期：2008-01-01 00:00:00

abstract：:Discriminating cell wall lytic enzymes from non lytic enzymes is a very important task for curing bacterial infections. In this paper, based on Chou's amphiphilic pseudo amino acid composition, we develop fisher-discriminant based classifier to predict cell wall lytic enzymes. Experiments show that 66.7% sensitivity w...

journal_title：Protein and peptide letters

authors： Ding H,Luo L,Lin H

更新日期：2009-01-01 00:00:00

abstract：:A 6.5 kDa serine protease inhibitor was purified by anion-exchange chromatography from the crude extract of the Inga umbratica seeds, containing inhibitor isoforms ranging from 6.3 to 6.7 kDa and protease inhibitors of approximately 19 kDa. The purified protein was characterized as a potent inhibitor against trypsin a...

journal_title：Protein and peptide letters

authors： Calderon LA,Teles RC,Leite JR,Franco OL,Grossi-de-Sá MF,Medrano FJ,Bloch C Jr,Freitas SM

更新日期：2005-08-01 00:00:00

abstract：:After several studies on prediction of mutation, we examine the effect of three sampling strategies, the sampling based on years, the sampling based on number of mutations, and the sampling based on the unpredictable portion of amino-acid pairs, on the prediction performance in H5N1 hemagglutinins. The results show th...

journal_title：Protein and peptide letters

authors： Wu G,Yan S

更新日期：2008-01-01 00:00:00

abstract：:Class I fusion glycoproteins of viruses are involved in the fusion between viral envelope and cell membrane. A region located in the N-terminal domain of these glycoproteins, called the fusion peptide, is essential for fusion. Fusion peptides are able to induce by themselves in vitro membrane fusion. In this paper, we...

journal_title：Protein and peptide letters

authors： Charloteaux B,Lorin A,Brasseur R,Lins L

更新日期：2009-01-01 00:00:00

abstract：:Heat shock proteins (HSPs) consist of a large group of proteins with negligible expressions under physiological conditions. Their expressions are highly induced under stress conditions and they are ubiquitously expressed in various tissues and organs. HSPs possess chaperone functions, thus facilitating the correct fol...

journal_title：Protein and peptide letters

authors： Lu WJ,Lee NP,Fatima S,Luk JM

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Development of proteins and peptides into drugs has been considered as a promising strategy to target certain diseases. However, only few proteins and peptides have been approved as new drugs into the market each year. One major problem is that proteins and peptides often exhibit short plasma half-life times...

journal_title：Protein and peptide letters

authors： Wu H,Huang J

更新日期：2018-01-01 00:00:00

abstract：:Proteases have been used not only for proteolysis but also in organic solvent-assisted religation processes. Here, we demonstrated the effect of salts on peptide bond resynthesis in Glutathione-S-transferase (GST) and have found it to be in the purview of the Hofmeister phenomena. Our results show that the efficiency ...

journal_title：Protein and peptide letters

authors： Kishan KV,Sharma A

更新日期：2010-01-01 00:00:00

abstract：:Bucain, a potent neurotoxin isolated from the venom of the Malayan krait (Bungarus candidus), induces paralysis and death. Its crystal structure has been determined at 2.10 A resolution and based on the molecular topology and hydrophobicity profile is structurally classified as a three-fingered alpha-neurotoxin posses...

journal_title：Protein and peptide letters

authors： Murakami MT,Kini RM,Arni RK

更新日期：2009-01-01 00:00:00

abstract：:The present study dealt with the isolation, identification and characterization of pigments from red snow samples of the Quito coastal front glacier (S 62º 27,217', W 059º 45,960') in Greenwich, Archipelago South Shetland, Antarctica, during summer 2013. As a strain of Shewanella was found to be the most common and ab...

journal_title：Protein and peptide letters

authors： Martín-Cerezo ML,García-López E,Cid C

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Microbe-Binding Peptides (MBPs) are currently being investigated to address the problem of antimicrobial resistance. Strategies enhancing their antimicrobial activity have been developed, including peptide dimerization. Here, we present an alternative approach based on peptide polymerization, yielding hapten...

journal_title：Protein and peptide letters

authors： Climacosa FMM,King RAN,Santos BMM,Caoili SEC

更新日期：2020-01-01 00:00:00

abstract：:Apomyoglobin (apoMb) folds through at least two partially folded forms that are detected both as transient intermediates during folding/unfolding kinetics or as stable intermediates at equilibrium. Here, I summarize the results of recent kinetic studies, which combined with detailed characterizations of equilibrium fo...

journal_title：Protein and peptide letters

authors： Jamin M

更新日期：2005-04-01 00:00:00

abstract：UNLABELLED:In this investigation a novel approach based on mucoadhesive superporous hydrogel hybrids (SPHHs) for topi- cal vaginal drug delivery for Metronidazole was tried. METHOD:SPHHs were synthesized by solution polymerization tech- nique. The synthesized SPHHs were characterized for tensile strength, swelling beh...

journal_title：Protein and peptide letters

authors： Chavda H,Chavada G,Patel J,Rangpadiya K,Patel C

更新日期：2014-01-01 00:00:00

abstract：:Most proteinaceous pores are characterized as ionic channels. However, some are also involved in protein translocation through phospholipidic membranes. This concept has evolved slowly in cell biology and in biophysics, requiring the development of adapted electrical and biochemical methods. Protein translocation in m...

journal_title：Protein and peptide letters

authors： Henry JP

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:Protealysin, a zinc metalloprotease of Serratia proteamaculans, is the prototype of a new group within the peptidase family M4. Protealysin-like proteases (PLPs) are widely spread in bacteria but are also found in fungi and archaea. The biological functions of PLPs have not been well studied, but published d...

journal_title：Protein and peptide letters

authors： Chukhontseva KN,Salnikov VV,Morenkov OS,Kostrov SV,Demidyuk IV

更新日期：2019-01-01 00:00:00

abstract：:Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...

journal_title：Protein and peptide letters

authors： Zhang XC,Wang WD,Wang JS,Pan JC,Zou GL

更新日期：2011-12-01 00:00:00

abstract：:The catalytic regions of Protein Kinases are known to have similarity in primary chains. However, it is not known whether there is a signature profile specific to a particular catalytic region? Whether the signature profile, if any, is unique to a protein kinases family in a particular species or in a group of species...

journal_title：Protein and peptide letters

authors： Joshi RR,Manda C

更新日期：2007-01-01 00:00:00

abstract：:Protein structure information is very useful for the confirmation of protein function. The protein structural class can provide information for protein 3D structure analysis, causing the conformation of the protein overall folding type plays a significant part in molecular biology. In this paper, we focus on the predi...

journal_title：Protein and peptide letters

authors： Liao B,Xiang Q,Li D

更新日期：2012-11-01 00:00:00

abstract：:We examined the production and secretion of fusion constructs containing alpha-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibili...

journal_title：Protein and peptide letters

authors： Bandsmer JC,Campbell TN,Cheyne I,Choy FY

更新日期：2006-01-01 00:00:00

abstract：:Calreticulin is a soluble endoplasmic reticulum chaperone, which has a relatively low melting point due to its remarkable structure with a relatively high content of flexible structural elements. Using far ultraviolet circular dichroism (CD) spectroscopy and a fluorescent dye binding thermal shift assay, we have inves...

journal_title：Protein and peptide letters

authors： Duus K,Larsen N,Tran TA,Güven E,Skov LK,Jespersgaard C,Gajhede M,Houen G

更新日期：2013-05-01 00:00:00

abstract：:Solid state nuclear magnetic resonance (NMR) has developed into one of the most informative and direct experimental approaches to the characterization of the molecular structures of amyloid fibrils, including those associated with Alzheimer's disease. In this article, essential aspects of solid state NMR methods are d...

journal_title：Protein and peptide letters

authors： Tycko R

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:NMAAP1 plays a role in regulating macrophage differentiation to the M1 type and exerting antitumoral functions. It is not clear what role and mechanism NMAAP1 does play in the reversal of macrophages from M1 to M2. METHODS:We detected the typing of macrophages with high or low expression of NMAAP1 by QPCR a...

journal_title：Protein and peptide letters

authors： Liu Q,Zhu P,Liu S,Tang M,Wang Y,Tian Y,Jin Z,Li D,Yan D

更新日期：2019-01-01 00:00:00

abstract：:AB204 is an Activin/BMP2 chimera, which has been found to exhibit a higher activity than Bone Morphogenetic Protein 2 (BMP2) in osteogenic activity. To prepare AB204 for its preclinical studies, AB204 has been characterized in various formulation buffers. We observed that AB204 purified by ion-exchange chromatography ...

journal_title：Protein and peptide letters

authors： Ahn C,Maslennikov I,Choi JY,Oh H,Cheong C,Choe S

更新日期：2014-05-01 00:00:00