Expression of active alpha-N-acetylglucosaminidase/TAT Chimerae in cultured Spodoptera frugiperda cells.

Abstract:

:We examined the production and secretion of fusion constructs containing alpha-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibility of utilizing TAT/alpha-N-acetylglucosaminidase chimerae in enzyme replacement therapy.

journal_name

Protein Pept Lett

authors

Bandsmer JC,Campbell TN,Cheyne I,Choy FY

doi

10.2174/092986606775974456

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

353-6

issue

4

eissn

0929-8665

issn

1875-5305

journal_volume

13

pub_type

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