Abstract:
:We examined the production and secretion of fusion constructs containing alpha-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibility of utilizing TAT/alpha-N-acetylglucosaminidase chimerae in enzyme replacement therapy.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Bandsmer JC,Campbell TN,Cheyne I,Choy FYdoi
10.2174/092986606775974456subject
Has Abstractpub_date
2006-01-01 00:00:00pages
353-6issue
4eissn
0929-8665issn
1875-5305journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Malignant tumor is still one of the important diseases worldwide, cytotoxic CD8+ T lymphocytes (CTLs) play an important role in killing tumor cells. OBJECTIVE:To enhance the immune response of our previously identified HLA-A2-restricted CTL epitopes, we designed a multiepitope YL66. METHOD:The fusion prote...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866524666170419152700
更新日期:2017-01-01 00:00:00
abstract::Porins are integral membrane proteins found in the outer membrane of bacteria, mitochondria and chloroplasts. Herein, we have reviewed sequence and structural understanding about bacterial porins. The first porin structure from Rhodobacter capsulatus at 1.8 Å resolution in 1991 till the recent structural advancement, ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866521666140827110755
更新日期:2015-01-01 00:00:00
abstract::Plasmepsin 4 (PM4) is a digestive vacuole enzyme found in all Plasmodium species examined to date. While P. falciparum has three additional aspartic proteinases in its digestive vacuole in addition to plasmepsin 4, other Plasmodium species have only PM4 in their digestive vacuole. Therefore, PM4 may be a good target f...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785849218
更新日期:2008-01-01 00:00:00
abstract::Human histatins are histidine-rich, low molecular weight salivary proteins that contribute to the immune system of the oral cavity. In this work, nucleotide sequences of the HIS1 (coding for histatin 1) and HIS2 (coding for histatin 3) genes, homologous to the human ones, have been sequenced and analysed in five prima...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610791306715
更新日期:2010-07-01 00:00:00
abstract::For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining (15)N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozy...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607781483741
更新日期:2007-01-01 00:00:00
abstract::Aspergillus sp. phytase contains five disulfide bonds. In order to elucidate their role, the reactivation and refolding of phytase in the absence and presence of dithiothreitol (DTT) was investigated. The results indicated that the disulfide bonds play an important role in the catalytic activity and conformational sta...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866054395752
更新日期:2005-08-01 00:00:00
abstract::Migraine is a common neurologic condition marked by recurrent episodes of headache. Its pathophysiology is highly complex involving neuronal, inflammatory and vascular mechanisms. The Renin-Angiotensin System (RAS) can modulate all these mechanism, being a potential pharmacological target for migraine treatment. We ca...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200129160136
更新日期:2020-01-01 00:00:00
abstract::Glycosyltransferases (GTs) are a ubiquitous group of enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Nucleotide-sugars, lipid phosphate sugars and phosphate sugars can act as activated donor molecules while acceptor substrate...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866511320080003
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND:CsaA is among the few chaperones which are present in both bacteria and archaea, but absent in eukaryotes. There are no reports on interactome analysis of CsaA from archaea, till date. Identification of binding partners of CsaA might be helpful in understanding CsaA-associated processes in Picrophilus torrid...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527999201126205131
更新日期:2020-11-26 00:00:00
abstract::Bucain, a potent neurotoxin isolated from the venom of the Malayan krait (Bungarus candidus), induces paralysis and death. Its crystal structure has been determined at 2.10 A resolution and based on the molecular topology and hydrophobicity profile is structurally classified as a three-fingered alpha-neurotoxin posses...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609789839304
更新日期:2009-01-01 00:00:00
abstract::α-Synuclein forms amyloid deposits in the dopaminergic neurons; a process that is believed to contribute to the Parkinson's disease. An emerging theme in amyloid research is the hypothesis that the toxic species produced during amyloid formation share common physic-chemical features and exert their effects by common m...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866522666150209142649
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Development of proteins and peptides into drugs has been considered as a promising strategy to target certain diseases. However, only few proteins and peptides have been approved as new drugs into the market each year. One major problem is that proteins and peptides often exhibit short plasma half-life times...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866525666180530122835
更新日期:2018-01-01 00:00:00
abstract::We used a de novo designed, beta-hairpin forming T1 peptide as a model to investigate the kinetics of peptide fibrogenesis by a combination of light scattering (LS), circular dichroism (CD), fluorescence, and atomic force microscopy (AFM). The results demonstrate that the T1 fibrogenesis undergoes a consecutive stepwi...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790963690
更新日期:2010-04-01 00:00:00
abstract::This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29/L37a, S29/L38, S29/S27 and S29/...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866033408273
更新日期:2003-02-01 00:00:00
abstract::Src is a non-receptor protein tyrosine kinase ubiquitously expressed in animals. It is involved in various cellular processes, including the innate immune response in mammals. However, less is known about the function of insect Src. Here we presented a homologue of Src in silkworm (Bombyx mori), named as BmSrc by phyl...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666161129114520
更新日期:2017-01-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a chronic disease with an autoimmunological background. RA is mostly characterized by systemic inflammation and injuries of synovial joints. There is a hypothesis that bacterial infections may be connected with development of the disease. It has been suggested that molecular mimicry betwee...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612803217123
更新日期:2012-11-01 00:00:00
abstract::Protein phosphatase magnesium-dependent 1, delta (PPM1D) is a member of the PPM1 (formerly PP2C) protein phosphatase family, and is induced in response to DNA damage. The overexpression of PPM1D is thought to exert oncogenic effects through the inhibition of tumor suppressor proteins. PPM1D shows high selectivity for ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785849236
更新日期:2008-01-01 00:00:00
abstract::Red blood cell (RBC) encapsulated L-asparaginase is a novel therapeutic for the treatment of asparagine auxotrophic malignancies. The enzyme-loaded red blood cells function as bioreactors to deplete bloodstream substrate. This delivery system provides improved pharmacodynamics with protection from circulating proteoly...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:
更新日期:2013-04-01 00:00:00
abstract::Natural, synthetic and analogues of peptides have shown prospects for application in cancer chemotherapy. Notably, some food protein-derived peptides are known to possess anticancer activities in cultured cancer cells, and also in animal cancer models via different mechanisms including induction of apoptosis, cell cyc...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866523666160816152755
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Nanosheets of SnO2 which an n-type semiconductor with a rutile-type crystalline structure are predominantly used as gas sensors. SnO2 nanosheets have a tetragonal crystal structure where growth along the c-axis is suppressed to form a sheet. The major exposed facets of SnO2 nanosheets have {110}, {101} and {...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666171206114429
更新日期:2018-01-01 00:00:00
abstract::Mutations of tumor suppressor protein p53 are present in almost about 50% of all cancers. It has been reported that the p53 mutations cause aggregation and subsequent loss of p53 function, leading to cancer progression. Here in this study we focus on the inhibitory effects of cationic osmolyte molecules acetylcholine ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866524666170123142858
更新日期:2017-01-01 00:00:00
abstract:UNLABELLED:In this investigation a novel approach based on mucoadhesive superporous hydrogel hybrids (SPHHs) for topi- cal vaginal drug delivery for Metronidazole was tried. METHOD:SPHHs were synthesized by solution polymerization tech- nique. The synthesized SPHHs were characterized for tensile strength, swelling beh...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866521666140807101402
更新日期:2014-01-01 00:00:00
abstract::Utilizing different computational methods; phosphorylation, O-GlcNAc modification and Yin Yang sites are predicted in HMGN-1. Prediction results suggest that interplay of phosphorylation and O-GlcNAc modification regulates binding and removal of HMGN-1 with the nucleosome and its translocation from nucleus to cytoplas...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608783489607
更新日期:2008-01-01 00:00:00
abstract::Coiled coils are important structural modules and domains of protein-protein interactions. Further, they provide the required framework to proteins, like kinesins, myosins and SNAREs, which are either structural or involved in transport of biomolecules. We provide an interactive webserver to measure the strength of in...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608783330314
更新日期:2008-01-01 00:00:00
abstract::The identification of interfaces in protein complexes is effective for the elucidation of protein function and helps us to understand their roles in biological processes. With the exponentially growing amount of protein sequence data, an exploration of new methods that predict protein interaction sites based solely on...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986613804725208
更新日期:2013-02-01 00:00:00
abstract::Numerous small molecules including cytokines primarily associated with immune response have been shown to play a role in normal mesenchymal stem cells (MSC) and tumour cells' communication. One characteristic that distinguishes MSC from fibroblast and other cells of mesenchymal origin is their pro-tumour migratory beh...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866522666150131123808
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Searching the biomarker from complex heterogeneous material for early detection of disease is a challenging task in the field of biomedical sciences. OBJECTIVE:The study has been arranged to explore the proteomics serum derived profiling of the differential expressed and low molecular weight protein in brea...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200110155609
更新日期:2020-01-01 00:00:00
abstract::The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-s...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612800494002
更新日期:2012-06-01 00:00:00
abstract::Many polyketides show biological activities and have thus been applied in clinics, as food additives, and in agriculture. Type II thioesterases (TEIIs) play an important role in polyketide biosynthesis. Most TEIIs belong to α/β-hydrolase family and usually contain a catalytic triad Ser-His-Asp. In polyketide biosynthe...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666160920095131
更新日期:2016-01-01 00:00:00
abstract::We have synthesised nine analogues of the antibacterial peptide anoplin with a peptoid residue at position 5 (H-GLLKXIKTLL-NH(2)). The most active compounds showed MIC-values of 12.5 and 25 microM against E.coli and S.aureus. These MIC-values are comparable with anoplin which showed 23 microM and 11 microM against E. ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609789055421
更新日期:2009-01-01 00:00:00