Statistics of catalytic domain sequences in protein kinases vs non-kinases.

abstract：BACKGROUND:Fruit juice clarification is a challenging aspect of beverage industry which needs to be addressed for economical and hygienic production of fruit juices. OBJECTIVE:Current study is focused on the complete purification, characterization and thermodynamic analysis of an efficient mannanase enzyme to analyze ...

journal_title：Protein and peptide letters

authors： Ikram Ul Haq,Shakoor S,Nawaz A,Arshad Y,Mukhtar H

更新日期：2020-09-16 00:00:00

abstract：:Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...

journal_title：Protein and peptide letters

authors： Zhang XC,Wang WD,Wang JS,Pan JC,Zou GL

更新日期：2011-12-01 00:00:00

abstract：:Cationic antimicrobial peptides (AMPs) are among the best studied antimicrobial factors expressed in the respiratory tract. AMPs are released by epithelial cells and immune cells into the airway surface liquid covering the epithelial surfaces of the lung where they act as endogenous antibiotics. Plenty of studies show...

journal_title：Protein and peptide letters

authors： Seiler F,Lepper PM,Bals R,Beisswenger C

更新日期：2014-04-01 00:00:00

abstract：:The mutation, conferring streptomycin and deoxyglucose resistance on cells, had profound effect on the kinetic and thermodynamic parameters inferring thermostabilization of beta-glucosidase from mutant 51 SM(r) of Cellulomonas biazotea. Free energy of activation for substrate binding, enthalpy and entropy of activatio...

journal_title：Protein and peptide letters

authors： Rajoka MI,Durrani IS,Khalid AM

更新日期：2005-04-01 00:00:00

abstract：:An alternative method for expressing the recombinant proteins in Escherichia coli is proposed. Unlike the ordinary induction protocol where the cells in the early- to mid-log phase are induced for the protein production, this alternative protocol utilizes the cells in the stationary phase. By using a glutathione S-tra...

journal_title：Protein and peptide letters

authors： Chae YK,Cho KS,Chun W,Lee K

更新日期：2003-08-01 00:00:00

abstract：:Highly efficient coupling reagents, N-methanesulphonyloxy-2-phenyl benzimidazole and N - p-toluenesulphonyloxy-2-phenyl benzimidazole were designed, synthesized and successfully applied in peptide coupling reactions. Their efficiency was evaluated by synthesizing a number of structurally different amides and peptides ...

journal_title：Protein and peptide letters

authors： Kokare ND,Nagawade RR,Rane VP,Shinde DB

更新日期：2007-01-01 00:00:00

abstract：:Protein-splicing inteins are widespread in nature and have found many applications in protein research and engineering. The mechanism of protein splicing typically requires a nucleophilic amino acid residue at both position 1 (first residue of intein) and position +1 (first residue after intein), however it was not cl...

journal_title：Protein and peptide letters

authors： Qi X,Wang J,Meng Q,Liu XQ

更新日期：2011-12-01 00:00:00

abstract：:A new galactose-specific lectin, named BBL, was purified from seeds of Bauhinia bauhinioides by precipitation with ammonium sulfate, followed by two steps of ion exchange chromatography. BBL haemagglutinated rabbit erythrocytes (native and treated with proteolytic enzymes) showing stability even after exposure to 60 °...

journal_title：Protein and peptide letters

authors： Silva HC,Bari AU,Pereira-Jénior FN,Simões RC,Barroso-Neto IL,Nobre CB,Pereira MG,Nascimento KS,Rocha BA,Delatorre P,Nagano CS,Assreuy AM,Cavada BS

更新日期：2011-04-01 00:00:00

abstract：BACKGROUND:Nanosheets of SnO2 which an n-type semiconductor with a rutile-type crystalline structure are predominantly used as gas sensors. SnO2 nanosheets have a tetragonal crystal structure where growth along the c-axis is suppressed to form a sheet. The major exposed facets of SnO2 nanosheets have {110}, {101} and {...

journal_title：Protein and peptide letters

authors： Nakazawa H,Seta Y,Hirose T,Masuda Y,Umetsu M

更新日期：2018-01-01 00:00:00

abstract：:Heat shock proteins (HSPs) consist of a large group of proteins with negligible expressions under physiological conditions. Their expressions are highly induced under stress conditions and they are ubiquitously expressed in various tissues and organs. HSPs possess chaperone functions, thus facilitating the correct fol...

journal_title：Protein and peptide letters

authors： Lu WJ,Lee NP,Fatima S,Luk JM

更新日期：2009-01-01 00:00:00

abstract：:We used a de novo designed, beta-hairpin forming T1 peptide as a model to investigate the kinetics of peptide fibrogenesis by a combination of light scattering (LS), circular dichroism (CD), fluorescence, and atomic force microscopy (AFM). The results demonstrate that the T1 fibrogenesis undergoes a consecutive stepwi...

journal_title：Protein and peptide letters

authors： Wang C,Sha Y

更新日期：2010-04-01 00:00:00

abstract：:The aerotolerance of the lactic - fermentative bacterium Streptococcus thermophilus is mainly based on the key antioxidant function of superoxide dismutase (StSOD). In this work, the comparison of recombinant StSOD (rStSOD) forms obtained from two different initiation triplets indicated that the enzyme from S. thermop...

journal_title：Protein and peptide letters

authors： De Vendittis A,Marco S,Di Maro A,Chambery A,Albino A,Masullo M,Michniewicz A,Parlato G,De Angelis A,De Vendittis E,Rullo R

更新日期：2012-03-01 00:00:00

abstract：BACKGROUND:In the last years, Antimicrobial Peptides (AMPs) and lipopeptides have received attention as promising candidates to treat infections caused by resistant microorganisms. OBJECTIVE:The main objective of this study was to investigate the effect of repetitive KLFK motifs and the attachment of aliphatic acids t...

journal_title：Protein and peptide letters

authors： Húmpola MV,Rey MC,Spontón PG,Simonetta AC,Tonarelli GG

更新日期：2019-01-01 00:00:00

abstract：:Two cell-penetrating peptides, a Pro-rich peptide derivative, acetyl-(Val-Arg-Leu-Pro-Pro-Pro)(3)-Gly-Cys amide, and an octaarginine derivative, acetyl-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys amide, were prepared by the solid phase method. Each peptide was coupled to the heterobifunctional cross-linking reagent, 6-mal...

journal_title：Protein and peptide letters

authors： Kida S,Eto Y,Yoshioka Y,Nakagawa S,Kawasaki K,Maeda M

更新日期：2010-02-01 00:00:00

abstract：:Bacterial ADP-ribosyltransferases (BADPRTs) are extensively contributed to determine the strain-specific virulence state and pathogenesis in human hosts. Understanding molecular evolution and functional diversity of the BADPRTs is an important standpoint to describe the fundamental behind in the vaccine designing for ...

journal_title：Protein and peptide letters

authors： Chellapandi P,Sakthishree S,Bharathi M

更新日期：2013-09-01 00:00:00

abstract：:The small heat shock protein SsHSP14.1 from the hyper-thermophilic archeaon, Sulfolobus solfataricus (S. solfataricus) was able to protect proteins from thermal aggregation and prevent enzymes from heat induced inactivation. According to the 3D (dimensional) structural model of SsHSP14.1 developed by us before, the re...

journal_title：Protein and peptide letters

authors： Wen ZZ,Wang YH,Yang B,Xie MQ,Chou KC

更新日期：2011-03-01 00:00:00

abstract：:Identification on protein folding types is always based on the 27-class folds dataset, which was provided by Ding & Dubchak in 2001. But with the avalanche of protein sequences, fold data is also expanding, so it will be the inevitable trend to improve the existing dataset and expand more folding types. In this paper,...

journal_title：Protein and peptide letters

authors： Liu L,Hu XZ,Liu XX,Wang Y,Li SB

更新日期：2012-04-01 00:00:00

abstract：:The identification of interfaces in protein complexes is effective for the elucidation of protein function and helps us to understand their roles in biological processes. With the exponentially growing amount of protein sequence data, an exploration of new methods that predict protein interaction sites based solely on...

journal_title：Protein and peptide letters

authors： Huang J,Deng R,Wang J,Wu H,Xiong Y,Wang X

更新日期：2013-02-01 00:00:00

abstract：:Kallikrein-related peptidases (KLKs) are trypsin-like and chymotrypsin-like serine proteases which are expressed in several tissues. Their activity is tightly controlled by inhibitors including members of the serine protease Kazal-type (SPINK) family. These enzymes are promising targets for the treatment of skin desqu...

journal_title：Protein and peptide letters

authors： Assis DM,Zalazar L,Juliano MA,De Castro R,Cesari A

更新日期：2013-10-01 00:00:00

abstract：:Prediction of thermophilic and mesophilic protein plays a crucial role in both biochemistry and bioengineering. In this study, a different mode of pseudo amino acid composition (PseAAC) was proposed to formulate the protein samples by integrating the amino acid composition, the physic chemical features, as well as the...

journal_title：Protein and peptide letters

authors： Wang D,Yang L,Fu Z,Xia J

更新日期：2011-07-01 00:00:00

abstract：BACKGROUND:Protein-carbohydrate interactions play vital roles in several biological processes in living organisms. The comparative analysis of binding site residues along with stabilizing residues in protein-carbohydrate complexes provides ample insights to understand the structure, function and recognition mechanism. ...

journal_title：Protein and peptide letters

authors： Shanmugam NRS,Selvin JFA,Veluraja K,Gromiha MM

更新日期：2018-01-01 00:00:00

abstract：:In mammals the M1 aminopeptidase family consists of nine different proteins, five of which are integral membrane proteins. The aminopeptidases are defined by two motifs in the catalytic domain; a zinc binding motif HEXXH-(X18)-E and an exopeptidase motif GXMEN. Aminopeptidases of this family are able to cleave a broad...

journal_title：Protein and peptide letters

authors： Albiston AL,Ye S,Chai SY

更新日期：2004-10-01 00:00:00

abstract：:Marine species are comprising about a half of the whole global biodiversity; the sea offers an enormous resource for novel bioactive compounds. Several of the marine origin species show multifunctional bioactivities and characteristics that are useful for a discovery and/or reinvention of biologically active compounds...

journal_title：Protein and peptide letters

authors： Chandra R,Parra R,Iqbal HM

更新日期：2017-01-01 00:00:00

abstract：:A thermostable uricase identified in Bacillus firmus DWD-33, which was isolated for the first time from soil, with an apparent molecular weight of 33.5 kDa was stable against oxidants and SDS. The highest expression yields were obtained in medium containing 0.8% maltose and 1.2% soybean powder as carbon and nitrogen s...

journal_title：Protein and peptide letters

authors： Kotb E

更新日期：2015-01-01 00:00:00

abstract：:Thioredoxin (TRX) catalyzes redox reactions via the reversible oxidation of the conserved active center CGPC and it is involved in multiple biological processes, some of them linked to redox activity while others not. TRX is a globular, thermodynamically stable and monomeric alpha/beta protein with a structure charact...

journal_title：Protein and peptide letters

authors： Vazquez DS,Delfino JM,Santos J

更新日期：2015-01-01 00:00:00

abstract：:Ewing's Sarcoma transcript-2 (EAT2) also known as SH2D1B is involved in regulation of signalling lymphocytic activation molecule (SLAM) family receptor functions. Cytoplasmic tails of SLAM family receptors contain tyrosine residues which mediate the downstream signal transduction through their phosphorylation. EAT2, c...

journal_title：Protein and peptide letters

authors： Taha M,Nezerwa E,Nam HJ

更新日期：2016-01-01 00:00:00

abstract：:A 6.5 kDa serine protease inhibitor was purified by anion-exchange chromatography from the crude extract of the Inga umbratica seeds, containing inhibitor isoforms ranging from 6.3 to 6.7 kDa and protease inhibitors of approximately 19 kDa. The purified protein was characterized as a potent inhibitor against trypsin a...

journal_title：Protein and peptide letters

authors： Calderon LA,Teles RC,Leite JR,Franco OL,Grossi-de-Sá MF,Medrano FJ,Bloch C Jr,Freitas SM

更新日期：2005-08-01 00:00:00

abstract：:We report the stepwise transformation of a linear peptide epitope recognized by the anti-transforming growth factor alpha monoclonal antibody Tab2 into peptomers and finally into peptoid analogs. The key experiment in this study is the substitution analysis in which each position of the peptide is exchanged by a set o...

journal_title：Protein and peptide letters

authors： Hoffmann B,Ast T,Polakowski T,Reineke U,Volkmer R

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Radical Probe Mass Spectrometry (RP-MS) describes a pioneering methodology in structural biology that enables the study of protein structures, their interactions, and dynamics on fast timescales (down to sub-milliseconds). Hydroxyl radicals (•OH) generated directly from water within aqueous solutions induce ...

journal_title：Protein and peptide letters

authors： Maleknia SD,Downard KM

更新日期：2019-01-01 00:00:00

abstract：:There is accumulating evidence that proteinuria is not merely a biomarker for the progression of chronic kidney disease (CKD), but also a mediator of this devastating disorder. Indeed, albumin, one of the major components found in proteinuria, causes proinflammatory and profibrotic changes in cultured proximal tubular...

journal_title：Protein and peptide letters

authors： Matsui T,Yamagishi S,Ueda S,Fukami K,Okuda S

更新日期：2010-01-01 00:00:00