Evaluation of synthetic cell-penetrating peptides, Pro-rich peptide and octaargine derivatives, as adenovirus vector carrier.

abstract：:C-terminal peptide alpha-thioesters are valuable intermediates in the synthesis/semisynthesis of proteins by native chemical ligation. They are prepared either by solid-phase peptide synthesis (SPPS) or biosynthetically by protein splicing techniques. The present paper reviews the different methods available for the c...

journal_title：Protein and peptide letters

authors： Camarero JA,Mitchell AR

更新日期：2005-11-01 00:00:00

abstract：:The cyclotides are a large family of plant proteins that have a cyclic backbone and a knotted arrangement of three conserved disulfide bonds. Despite the apparent complexity of their cystine knot motif it is possible to efficiently fold these proteins, as exemplified by oxidative folding studies on the prototypic cycl...

journal_title：Protein and peptide letters

authors： Craik DJ,Daly NL

更新日期：2005-02-01 00:00:00

abstract：:Neuronal tau, through its proline-rich domain and the microtubule binding domain, binds to RNA non-sequence-specifically via electrostatic interaction. This binding inhibits the activity of tau. Tau and RNA were also found to co-localize in SH-SY5Y cells suggesting that RNA has opportunities to interact with tau in ce...

journal_title：Protein and peptide letters

authors： Wang X,Wang D,Zhao J,Qu M,Zhou X,He H,He R

更新日期：2006-01-01 00:00:00

abstract：:Increasing antibiotic resistance has led to an urgent need for new therapeutic approaches. Host defense peptides are known to be antimicrobial and have revealed broad immunomodulatory functions for both innate and adaptive immunity. This review will focus on the role of host defense peptides in infection and immune re...

journal_title：Protein and peptide letters

authors： Hirsch T,Jacobsen F,Steinau HU,Steinstraesser L

更新日期：2008-01-01 00:00:00

abstract：:The Saccaromices cerevisiae D-serine dehydratase is a pyridoxal 5'-phosphate dependent enzyme that requires zinc for its function. It catalyses the conversion of D-serine into pyruvate and ammonia with the K(m) and k(cat) values of 0.39 mM and 13.1 s(-1) respectively. In this work, a new methodology for monitoring D-s...

journal_title：Protein and peptide letters

authors： Staiano M,Strianese M,Varriale A,Di Giovanni S,di Mase DS,Dell'Angelo V,Ruggiero G,Labella T,Pellecchia C,D'Auria S

更新日期：2012-06-01 00:00:00

abstract：BACKGROUND:OPT3s are involved in the transport of Fe from xylem to phloem, in loading Fe into phloem, and in the transmission of shoot-to-root iron signaling. Yet, apart from Arabidopsis, little is known about these transporters'functions in other plant species. OBJECTIVE:OPT3 proteins of several plant species were ch...

journal_title：Protein and peptide letters

authors： Kurt F

更新日期：2020-06-25 00:00:00

abstract：BACKGROUND:Microbe-Binding Peptides (MBPs) are currently being investigated to address the problem of antimicrobial resistance. Strategies enhancing their antimicrobial activity have been developed, including peptide dimerization. Here, we present an alternative approach based on peptide polymerization, yielding hapten...

journal_title：Protein and peptide letters

authors： Climacosa FMM,King RAN,Santos BMM,Caoili SEC

更新日期：2020-01-01 00:00:00

abstract：:Porins are integral membrane proteins found in the outer membrane of bacteria, mitochondria and chloroplasts. Herein, we have reviewed sequence and structural understanding about bacterial porins. The first porin structure from Rhodobacter capsulatus at 1.8 Å resolution in 1991 till the recent structural advancement, ...

journal_title：Protein and peptide letters

authors： Kumar A,Bhandari A,Krishnaswamy S

更新日期：2015-01-01 00:00:00

abstract：:DNA fragmentation factor 45 (DFF45) regulates DNase DFF40 as its inhibitor and chaperone. It was reported that the N-terminal domain (NTD) of DFF45 alone is disordered and DFF40 is necessary as a mutual chaperone for the folding of NTD. However, here we reported the crystallization of DFF45 NTD. These crystals diffrac...

journal_title：Protein and peptide letters

authors： Li T,Li X,Yang W,Rao Z,Zhai Zh

更新日期：2003-04-01 00:00:00

abstract：:Polymyositis is an inflammatory myopathy characterized by muscle invasion of T-cells penetrating the basal lamina and displacing the plasma membrane of normal muscle fibers. In order to understand the different adhesive mechanisms at the T-cell surface, Schubert randomly selected 19 proteins expressed at the T-cell su...

journal_title：Protein and peptide letters

authors： Li FZ,Gao F

更新日期：2012-08-01 00:00:00

abstract：:The study of rat proteins is an indispensable task in experimental medicine and drug development. The function of a rat protein is closely related to its subcellular location. Based on the above concept, we construct the benchmark rat proteins dataset and develop a combined approach for predicting the subcellular loca...

journal_title：Protein and peptide letters

authors： Shi R,Xu C

更新日期：2011-06-01 00:00:00

abstract：:The immunoglobulin fold (Ig-fold) is a widespread structural motif that is detected in a variety of proteins involved in diversified biological processes. The Ig-fold contains 70-110 residues that are assembled in a characteristic sandwich-like structure formed by two facing β-sheets each made of antiparallel β-strand...

journal_title：Protein and peptide letters

authors： Berisio R,Ciccarelli L,Squeglia F,De Simone A,Vitagliano L

更新日期：2012-10-01 00:00:00

abstract：:The etiology of multiple sclerosis (MS) remains unclear. To determine if autoantibodies to myelin basic protein (MBP) are produced during parvovirus B19 infection, a competitive ELISA was performed using plasma from MS patients exhibiting high IgG titers for parvovirus. Our results showed the addition of MBP decreased...

journal_title：Protein and peptide letters

authors： Thomas G,Rael L,Shimonkevitz R,Melamed I,Bar-Or D

更新日期：2006-01-01 00:00:00

abstract：:Red blood cell (RBC) encapsulated L-asparaginase is a novel therapeutic for the treatment of asparagine auxotrophic malignancies. The enzyme-loaded red blood cells function as bioreactors to deplete bloodstream substrate. This delivery system provides improved pharmacodynamics with protection from circulating proteoly...

journal_title：Protein and peptide letters

authors： Agrawal V,Woo JH,Borthakur G,Kantarjian H,Frankel AE

更新日期：2013-04-01 00:00:00

abstract：:Self-Interacting Proteins (SIPs), whose two or more copies can interact with each other, have significant roles in cellular functions and evolution of Protein Interaction Networks (PINs). Knowing whether a protein can act on itself is important to understand its functions. Previous studies on SIPs have focused on thei...

journal_title：Protein and peptide letters

authors： Qu J,Zhao Y,Zhang L,Cai SB,Ming Z,Wang CC

更新日期：2020-01-01 00:00:00

abstract：:Thioredoxin (TRX) catalyzes redox reactions via the reversible oxidation of the conserved active center CGPC and it is involved in multiple biological processes, some of them linked to redox activity while others not. TRX is a globular, thermodynamically stable and monomeric alpha/beta protein with a structure charact...

journal_title：Protein and peptide letters

authors： Vazquez DS,Delfino JM,Santos J

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:The biocatalytic oxidation of UDP-glucose in the presence of NAD+ is catalyzed by UDP-glucose dehydrogenases. OBJECTIVES:The main objective of this study was the characterization of a UDP-glucose dehydrogenase (AmUGD) from Akkermansia muciniphila, a bacterium originally isolated from human faeces in an anae...

journal_title：Protein and peptide letters

authors： Wei S,Zhang XY,Sun Y,Conway LP,Liu L

更新日期：2017-01-01 00:00:00

abstract：:This work describes the investigations of electrochemical interactions of naphthalene acetic acid (NAA) with stabilized lipid films supported on a methacrylate polymer on a glass fiber filter with incorporated auxin-binding protein 1 receptor for the development of a biosensor for the rapid detection of this compound ...

journal_title：Protein and peptide letters

authors： Nikolelis DP,Ntanos N,Nikoleli GP,Tampouris K

更新日期：2008-01-01 00:00:00

abstract：:Transgelin was solubly expressed in E. coli. Crystals of transgelin have been grown at 291K using sodium formate or PEG-4000 as precipitants. X-ray diffraction by the crystal extends to 2.3 A resolution. The crystal belongs to the space group P2(1), with the unit cell parameters a=39.3, b=61.9, c=56.0 A and beta=90.2 ...

journal_title：Protein and peptide letters

authors： Liao X,Li S,Lou Z,Chen Z,Bartlam M

更新日期：2007-01-01 00:00:00

abstract：:From the extensive analysis, we identified three highly conserved sequence segments in HCV NS5A proteins and one binding motif in human proteins. The binding motif of human proteins often forms a full helix or an extended strand-loop structure, and is in good agreement with the experimental findings of previous studie...

journal_title：Protein and peptide letters

authors： Zhang GZ,Han K

更新日期：2008-01-01 00:00:00

abstract：:Human ankyrin repeat and suppressor of cytokine signaling box protein 9 (hASB9), a subunit of an Elongin C-cullin-SOCS box (ECS) E3 ubiquitin ligase complex, is believed to be involved in specific substrate-recognition for ubiquitination and degradation. In fact, this specific substrate-recognition is determined by th...

journal_title：Protein and peptide letters

authors： Fei X,Zhang Y,Gu X,Qiu R,Mao Y,Ji C

更新日期：2009-01-01 00:00:00

abstract：:The stability of Merrifield linker in Fmoc deprotection process was quantitatively investigated by establishing working curve of two major decomposition components from two resin bound dipeptide models. By sampling reaction solution and analyzing with RP-HPLC, decomposition rate was determined. The results indicated t...

journal_title：Protein and peptide letters

authors： Yang X,Lin H,Lu W,Wang D

更新日期：2013-02-01 00:00:00

abstract：:The mutation, conferring streptomycin and deoxyglucose resistance on cells, had profound effect on the kinetic and thermodynamic parameters inferring thermostabilization of beta-glucosidase from mutant 51 SM(r) of Cellulomonas biazotea. Free energy of activation for substrate binding, enthalpy and entropy of activatio...

journal_title：Protein and peptide letters

authors： Rajoka MI,Durrani IS,Khalid AM

更新日期：2005-04-01 00:00:00

abstract：:The formation of local structure, in short peptides has been probed by examining cleavage patterns and rates of proteolysis of designed sequences with a high tendency to form beta-hairpin structures. Three model sequences which bear fluorescence donor and acceptor groups have been investigated: [see text]. Fluorescenc...

journal_title：Protein and peptide letters

authors： Saikumari YK,Ravindra G,Balaram P

更新日期：2006-01-01 00:00:00

abstract：:After consideration of the tertiary structure of the pY+3 binding pocket in SH2 domains, isoleucine of Ac-pYEEIE was replaced with various unnatural aliphatic amino acids and the binding affinities for Lck, Src, and Fyn SH2 domains were measured. We determined the characteristics of the amino acid at the pY+3 position...

journal_title：Protein and peptide letters

authors： Lee KH,Park SH

更新日期：2004-12-01 00:00:00

abstract：:Galanin is a neuropeptide that is widely distributed in the central and peripheral nervous systems. Some small cell lung carcinoma (SCLC) cell lines such as SBC-3A release only the high-molecular-mass form, with lower molecular mass forms being undetectable. To investigate the mechanism of processing of progalanin to ...

journal_title：Protein and peptide letters

authors： Yamamoto H,Iguchi K,Ohno S,Yokogawa T,Nishikawa K,Hoshino M

更新日期：2011-10-01 00:00:00

abstract：:A Surface Plasmon Resonance Imaging (SPRI) sensor has been developed for highly selective determination of cathepsin D (Cat D) or/and E (Cat E). The sensor contains immobilised pepstatin A, which binds aspartyl proteases from solution. Pepstatin A activated with N-Hydroxysuccinimide (NHS) and N-Ethyl-N'-(3-dimethylami...

journal_title：Protein and peptide letters

authors： Gorodkiewicz E,Regulska E

更新日期：2010-09-01 00:00:00

abstract：:Sry-related box (Sox) transcription factors share a conserved high-mobility-group box domain (HMG-domain) that binds DNA in the minor groove and bends DNA for further assembly of transcriptional machineries. During organogenesis, each member of the Sox family triggers a specific cell lineage differentiation, indicatin...

journal_title：Protein and peptide letters

authors： Gao N,Jiang W,Gao H,Cheng Z,Qian H,Si S,Xie Y

更新日期：2013-04-01 00:00:00

abstract：BACKGROUND:Protealysin, a zinc metalloprotease of Serratia proteamaculans, is the prototype of a new group within the peptidase family M4. Protealysin-like proteases (PLPs) are widely spread in bacteria but are also found in fungi and archaea. The biological functions of PLPs have not been well studied, but published d...

journal_title：Protein and peptide letters

authors： Chukhontseva KN,Salnikov VV,Morenkov OS,Kostrov SV,Demidyuk IV

更新日期：2019-01-01 00:00:00

abstract：:In general, halophilic proteins are stable only in the presence of salts at high concentrations. Not only is high salt concentration important for structural stability of halophilic proteins, but also refolding of a denatured halophilic protein requires high salt concentration. This review summarizes the importance of...

journal_title：Protein and peptide letters

authors： Arakawa T,Tokunaga M

更新日期：2004-04-01 00:00:00