Abstract:
:The stability of Merrifield linker in Fmoc deprotection process was quantitatively investigated by establishing working curve of two major decomposition components from two resin bound dipeptide models. By sampling reaction solution and analyzing with RP-HPLC, decomposition rate was determined. The results indicated that either α-amino acid or β-amino acid anchored Merrifield linker was endurable for Fmoc strategy peptide synthesis in common de-Fmoc conditions such as 20% piperidine/DMF and 2% DBU/2% piperidine/DMF under room temperature treatments. However, Fmoc-deprotection with microwave assistance of α-amino acid anchored peptide resin with 20% piperidine/DMF more than 20 times or β-amino acid anchored peptide resin with 2% DBU/2% piperidine/DMF more than 30 times is not recommended. Feasibility of the proposed compatibility was verified by design and synthesis of a thymic humoral factor derived peptide via Fmoc strategy on Merrifield resin. Thus by choosing moderate de-Fmoc protocol, Merrifield resin is feasible for Fmoc strategy oligopeptide synthesis.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Yang X,Lin H,Lu W,Wang Ddoi
10.2174/092986613804725343subject
Has Abstractpub_date
2013-02-01 00:00:00pages
140-5issue
2eissn
0929-8665issn
1875-5305pii
PPL-EPUB-20120807-1journal_volume
20pub_type
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journal_title:Protein and peptide letters
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