Development of an electrochemical biosensor for the rapid detection of naphthalene acetic acid in fruits by using air stable lipid films with incorporated auxin-binding protein 1 receptor.

Abstract:

:This work describes the investigations of electrochemical interactions of naphthalene acetic acid (NAA) with stabilized lipid films supported on a methacrylate polymer on a glass fiber filter with incorporated auxin-binding protein 1 receptor for the development of a biosensor for the rapid detection of this compound in fruits. NAA was injected into the flowing streams of a carrier electrolyte solution, the flow of the electrolyte solution stops and an ion current transient was obtained; the magnitude of the signal was correlated to NAA concentration, which could be determined at the micromolar level. NAA preconcentrates at the lipid membrane surface which causes dynamic alterations of the electrostatic fields and phase structure of membranes. The response times were ca. 5 min and naphthalene acetic acid was determined at concentration levels of microM. The effect of potent interferences included a wide range of compounds. The results showed no interferences from these compounds in concentration levels usually found in real samples. The method was applied for the determination of NAA in fruits and the reproducibility of the method was checked in about 100 samples. A quantitative method for the detection of NAA in fruits that can be complimentary to HPLC methods is provided in the present paper. These lipid films can be used as portable sensors for the rapid detection of NAA in fruits by non-skilled personnel.

journal_name

Protein Pept Lett

authors

Nikolelis DP,Ntanos N,Nikoleli GP,Tampouris K

doi

10.2174/092986608785203692

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

789-94

issue

8

eissn

0929-8665

issn

1875-5305

journal_volume

15

pub_type

杂志文章
  • Influence of selected factors on veins' permeability for albumin in vitro.

    abstract::We present the results of a study on the influence of albumin and prolactin concentrations and intravascular fluid pH on vein permeability for albumin. Permeability conditions were simulated depending on albumin concentration, pH value and prolactin concentration. In research model an in vitro method was applied using...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/09298665113209990082

    authors: Dolińska B,Caban A,Cierpka L,Ryszka F

    更新日期:2014-01-01 00:00:00

  • Autoantibody reaction to myelin basic protein by plasma parvovirus B19 IgG in MS patients.

    abstract::The etiology of multiple sclerosis (MS) remains unclear. To determine if autoantibodies to myelin basic protein (MBP) are produced during parvovirus B19 infection, a competitive ELISA was performed using plasma from MS patients exhibiting high IgG titers for parvovirus. Our results showed the addition of MBP decreased...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986606775101715

    authors: Thomas G,Rael L,Shimonkevitz R,Melamed I,Bar-Or D

    更新日期:2006-01-01 00:00:00

  • Structure, activities and biomedical applications of human butyrylcholinesterase.

    abstract::Human butyrylcholinesterase (BuChE) is a serine enzyme present in most organs and plasma. No clear physiological function has yet been assigned to BuChE, but it is a pharmacologically and toxicologically important enzyme that plays a role in degradation of numerous ester-containing drugs and poisonous esters. Thus, Bu...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986609789071207

    authors: Masson P,Carletti E,Nachon F

    更新日期:2009-01-01 00:00:00

  • Antibodies and their multivalent constructs for cancer therapy.

    abstract::In 2008 cancer was identified by the World Health Organization (WHO) as one of four threats to human health and development. Since the early published reports of the first chemotherapeutic, mustine, in 1946, the anti-cancer drug and development industry has grown into a multi-billion dollar business enterprise. Worldw...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/0929866521666140626102802

    authors: Li Q,Yi L,Marek P

    更新日期:2014-01-01 00:00:00

  • tRNase Z.

    abstract::Endonuclease tRNase Z catalyzes the generation of the mature 3' end of tRNA precursors through specific endonucleolytic cleavage. The enzyme has been characterized from organisms representative of all domains of life as well as from organelles, and the crystal structure of three bacterial enzymes has been determined. ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986607779816050

    authors: Ceballos M,Vioque A

    更新日期:2007-01-01 00:00:00

  • Sperm chromatin protamination: an endocrine perspective.

    abstract::During spermiogenesis, the elongating rat spermatid chromatin undergoes a gradual process of condensation which is initiated in the round spermatids at "step 7" of cytodifferentation in stage VII and extending to elongated spermatids at "step 19" of cytodifferentiation in stage VIII. The mechanism of chromatin condens...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986611795714005

    authors: Gill-Sharma MK,Choudhuri J,D'Souza S

    更新日期:2011-08-01 00:00:00

  • A novel thermoacidophilic cellulase from Alicyclobacillus acidocaldarius.

    abstract::A novel cellulase was isolated from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius ATCC27009 grown in medium containing carboxymethylcellulose. The enzyme is a glycosylated monomer of 56.2 kDa, relatively thermostable, with optimal pH and temperature of 4.0 and 65 degrees C, respectively. Enzymatic as...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986608785849209

    authors: Morana A,Esposito A,Maurelli L,Ruggiero G,Ionata E,Rossi M,La Cara F

    更新日期:2008-01-01 00:00:00

  • Efficient Expression and Purification of Cryptochrome1 from Columbia livia in E. coli.

    abstract:BACKGROUND:Cryptochrome is a flavin-binding blue-light photoreceptor that functions in growth and development in plants, the circadian clock in animals and navigation in birds. However, a lack of purified cryptochrome has hindered studies of the structure and function of this protein. In this study, we obtained a subst...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866525666181004112308

    authors: Yuan X,Wang W,Wu W,Wang J

    更新日期:2018-01-01 00:00:00

  • Biochemical characterization of a new maize (Zea mays L.) peptide growth factor.

    abstract::Coordination of cell growth and cell division is very important for living organisms in order for these to develop harmonically. The present research is concerned with the purification and characterization of a new peptide hormone, namely ZmIGF (Zea mays insulin-like growth factor), which regulates growth and cell div...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986611794328636

    authors: Rodríguez-López CD,Rodríguez-Romero A,Aguilar R,de Jiménez ES

    更新日期:2011-01-01 00:00:00

  • Expressed protein ligation: a new tool for the biosynthesis of cyclic polypeptides.

    abstract::The present paper reviews the use of expressed protein ligation for the biosynthesis of backbone cyclized polypeptides. This general method allows the in vivo and in vitro biosynthesis of cyclic polypeptides using recombinant DNA expression techniques. Biosynthetic access to backbone cyclic peptides opens the possibil...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/0929866054864274

    authors: Kimura R,Camarero JA

    更新日期:2005-11-01 00:00:00

  • SpliVert: A Protein Multiple Sequence Alignment Refinement Method Based on Splitting-Splicing Vertically.

    abstract:BACKGROUND:Multiple Sequence Alignment (MSA) is a fundamental task in bioinformatics and is required for many biological analysis tasks. The more accurate the alignments are, the more credible the downstream analyses. Most protein MSA algorithms realign an alignment to refine it by dividing it into two groups horizonta...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866526666190806143959

    authors: Zhan Q,Fu Y,Jiang Q,Liu B,Peng J,Wang Y

    更新日期:2020-01-01 00:00:00

  • COILCHECK: an interactive server for the analysis of interface regions in coiled coils.

    abstract::Coiled coils are important structural modules and domains of protein-protein interactions. Further, they provide the required framework to proteins, like kinesins, myosins and SNAREs, which are either structural or involved in transport of biomolecules. We provide an interactive webserver to measure the strength of in...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986608783330314

    authors: Alva V,Syamala Devi DP,Sowdhamini R

    更新日期:2008-01-01 00:00:00

  • Sequence and structural perspectives of bacterial β-stranded porins.

    abstract::Porins are integral membrane proteins found in the outer membrane of bacteria, mitochondria and chloroplasts. Herein, we have reviewed sequence and structural understanding about bacterial porins. The first porin structure from Rhodobacter capsulatus at 1.8 Å resolution in 1991 till the recent structural advancement, ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866521666140827110755

    authors: Kumar A,Bhandari A,Krishnaswamy S

    更新日期:2015-01-01 00:00:00

  • Evaluation of Stemness Maintenance Properties of the Recombinant Human Laminin α2 LG1-3 Domains in Human Mesenchymal Stem Cells.

    abstract:BACKGROUND:Laminin, a member of the Extracellular Matrix (ECM), is a glycoprotein that is used as a factor that affects cell adhesion, proliferation, survival, and differentiation. Of these, five globular domains (LG domains) of the alpha chain play an important role in influencing the cell by binding to the integrin. ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866526666190617091155

    authors: Kim JE,Seo HJ,Lee S,Jang JH

    更新日期:2019-01-01 00:00:00

  • Sialic acid recognition of the pandemic influenza 2009 H1N1 virus: binding mechanism between human receptor and influenza hemagglutinin.

    abstract::Quantum mechanical fragment molecular orbital calculations have been performed for receptor binding of the hemagglutinin protein of the recently pandemic influenza 2009 H1N1, A/swine/Iowa/1930, and A/Puerto Rico/8/1934 viruses to α2-6 linked sialyloligosaccharides, as analogs of human receptors. The strongest receptor...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986611794927893

    authors: Fukuzawa K,Omagari K,Nakajima K,Nobusawa E,Tanaka S

    更新日期:2011-05-01 00:00:00

  • Protein-protein interactions in membranes.

    abstract::In this article we review the current status of our understanding of membrane mediated interactions from theory and experiment. Phenomenological mean field and molecular models will be discussed and compared to recent experimental results from dynamical neutron scattering and atomic force microscopy. ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986611794653941

    authors: Armstrong CL,Sandqvist E,Rheinstädter MC

    更新日期:2011-04-01 00:00:00

  • Structural and dynamic properties of incomplete immunoglobulin-like fold domains.

    abstract::The immunoglobulin fold (Ig-fold) is a widespread structural motif that is detected in a variety of proteins involved in diversified biological processes. The Ig-fold contains 70-110 residues that are assembled in a characteristic sandwich-like structure formed by two facing β-sheets each made of antiparallel β-strand...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986612802762732

    authors: Berisio R,Ciccarelli L,Squeglia F,De Simone A,Vitagliano L

    更新日期:2012-10-01 00:00:00

  • Analysis of Antioxidant Enzyme Activity and Antioxidant Genes Expression During Germination of Two Different Genotypes of Lolium multiflorum Under Salt Tolerance.

    abstract::Annual ryegrass (Lolium multiflorum) is widely used as a cool-season forage grass for its luxuriant growth, palatable and high digestible. To investigate the salt tolerance mechanism in annual ryegrass under salt stress, salt-tolerant genotype 'R102-3' and salt-sensitive genotype 'Tetragold' were subject to 300mmol/L ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866523666160314153618

    authors: Wang X,Ma X,Xinquan-Zhang,Linkai-Huang,Li Z,Nie WX

    更新日期:2016-01-01 00:00:00

  • Characterization of Activin/BMP2 chimera, AB204, formulated for preclinical studies.

    abstract::AB204 is an Activin/BMP2 chimera, which has been found to exhibit a higher activity than Bone Morphogenetic Protein 2 (BMP2) in osteogenic activity. To prepare AB204 for its preclinical studies, AB204 has been characterized in various formulation buffers. We observed that AB204 purified by ion-exchange chromatography ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986652105140218110302

    authors: Ahn C,Maslennikov I,Choi JY,Oh H,Cheong C,Choe S

    更新日期:2014-05-01 00:00:00

  • Structure formation in short designed peptides probed by proteolytic cleavage.

    abstract::The formation of local structure, in short peptides has been probed by examining cleavage patterns and rates of proteolysis of designed sequences with a high tendency to form beta-hairpin structures. Three model sequences which bear fluorescence donor and acceptor groups have been investigated: [see text]. Fluorescenc...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986606776819501

    authors: Saikumari YK,Ravindra G,Balaram P

    更新日期:2006-01-01 00:00:00

  • The folding process of apomyoglobin.

    abstract::Apomyoglobin (apoMb) folds through at least two partially folded forms that are detected both as transient intermediates during folding/unfolding kinetics or as stable intermediates at equilibrium. Here, I summarize the results of recent kinetic studies, which combined with detailed characterizations of equilibrium fo...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/0929866053587174

    authors: Jamin M

    更新日期:2005-04-01 00:00:00

  • Prediction of rat protein subcellular localization with pseudo amino acid composition based on multiple sequential features.

    abstract::The study of rat proteins is an indispensable task in experimental medicine and drug development. The function of a rat protein is closely related to its subcellular location. Based on the above concept, we construct the benchmark rat proteins dataset and develop a combined approach for predicting the subcellular loca...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986611795222768

    authors: Shi R,Xu C

    更新日期:2011-06-01 00:00:00

  • A solution study of the interaction of the Cu(II) ions with HisGlyGlyTrp tetrapeptide and its evaluation as superoxide dismutase mimetic complex.

    abstract::The superoxide anion radical is a highly reactive toxic species produced during the metabolic processes. A number of copper (II) complexes with amino acids and peptides are known to show superoxide dismutase (SOD) like activity. The design and application of synthetic low molecular weight metal complexes as SOD mimics...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986611797642724

    authors: Singh RK,Srivastava VC,Singh UP

    更新日期:2011-12-01 00:00:00

  • The "Tilted Peptide Theory" links membrane insertion properties and fusogenicity of viral fusion peptides.

    abstract::Class I fusion glycoproteins of viruses are involved in the fusion between viral envelope and cell membrane. A region located in the N-terminal domain of these glycoproteins, called the fusion peptide, is essential for fusion. Fusion peptides are able to induce by themselves in vitro membrane fusion. In this paper, we...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986609788681724

    authors: Charloteaux B,Lorin A,Brasseur R,Lins L

    更新日期:2009-01-01 00:00:00

  • Identification and Analysis of Key Residues Involved in Folding and Binding of Protein-carbohydrate Complexes.

    abstract:BACKGROUND:Protein-carbohydrate interactions play vital roles in several biological processes in living organisms. The comparative analysis of binding site residues along with stabilizing residues in protein-carbohydrate complexes provides ample insights to understand the structure, function and recognition mechanism. ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866525666180221122529

    authors: Shanmugam NRS,Selvin JFA,Veluraja K,Gromiha MM

    更新日期:2018-01-01 00:00:00

  • Evidences of monomer, dimer and trimer of recombinant human cyclophilin A.

    abstract::Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986611797642670

    authors: Zhang XC,Wang WD,Wang JS,Pan JC,Zou GL

    更新日期:2011-12-01 00:00:00

  • Protein Footprinting with Radical Probe Mass Spectrometry- Two Decades of Achievement.

    abstract:BACKGROUND:Radical Probe Mass Spectrometry (RP-MS) describes a pioneering methodology in structural biology that enables the study of protein structures, their interactions, and dynamics on fast timescales (down to sub-milliseconds). Hydroxyl radicals (•OH) generated directly from water within aqueous solutions induce ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/0929866526666181128124241

    authors: Maleknia SD,Downard KM

    更新日期:2019-01-01 00:00:00

  • Proteome analysis of rice root plasma membrane and detection of cold stress responsive proteins.

    abstract::To investigate the function of plant plasma membrane, proteins of rice plasma membrane were analyzed and the proteins changed by cold stress were identified. Plasma membrane proteins were purified with an aqueous two-phase partitioning method from root of rice seedlings, and activity of specific H(+)-ATPase localized ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986609788490140

    authors: Hashimoto M,Toorchi M,Matsushita K,Iwasaki Y,Komatsu S

    更新日期:2009-01-01 00:00:00

  • Predicting protein crystallizability and nucleation.

    abstract::The outcome of protein crystallization attempts is often uncertain due to inherent features of the protein or to the crystallization process that are not fully under control of the experimentalist. The aim of this contribution is to propose user-friendly tools that can increase the success rate of a protein crytalliza...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986612800793127

    authors: Sánchez-Puig N,Sauter C,Lorber B,Giegé R,Moreno A

    更新日期:2012-07-01 00:00:00

  • Topical vaginal drug delivery system based on superporous hydrogel hybrids.

    abstract:UNLABELLED:In this investigation a novel approach based on mucoadhesive superporous hydrogel hybrids (SPHHs) for topi- cal vaginal drug delivery for Metronidazole was tried. METHOD:SPHHs were synthesized by solution polymerization tech- nique. The synthesized SPHHs were characterized for tensile strength, swelling beh...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866521666140807101402

    authors: Chavda H,Chavada G,Patel J,Rangpadiya K,Patel C

    更新日期:2014-01-01 00:00:00