Abstract:
:A new galactose-specific lectin, named BBL, was purified from seeds of Bauhinia bauhinioides by precipitation with ammonium sulfate, followed by two steps of ion exchange chromatography. BBL haemagglutinated rabbit erythrocytes (native and treated with proteolytic enzymes) showing stability even after exposure to 60 °C for an hour. The lectin haemagglutinating activity was optimum between pH 8.0 and 9.0 and inhibited after incubation with D-galactose and its derivatives, especially α-methyl-D-galactopyranoside. The pure protein possessed a molecular mass of 31 kDa by SDS-PAGE and 28.310 Da by mass spectrometry. The lectin pro-inflammatory activity was also evaluated. The s.c. injection of BBL into rats induced a dose-dependent paw edema, an effect that occurred via carbohydrate site interaction and was significantly reduced by L-NAME, suggesting an important participation of nitric oxide in the late phase of the edema. These findings indicate that BBL can be used as a tool to better understand the mechanisms involved in inflammatory responses.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Silva HC,Bari AU,Pereira-Jénior FN,Simões RC,Barroso-Neto IL,Nobre CB,Pereira MG,Nascimento KS,Rocha BA,Delatorre P,Nagano CS,Assreuy AM,Cavada BSdoi
10.2174/092986611794653987subject
Has Abstractpub_date
2011-04-01 00:00:00pages
396-402issue
4eissn
0929-8665issn
1875-5305pii
BSP/ PPL/ E pub/0252journal_volume
18pub_type
杂志文章abstract:BACKGROUND:OPT3s are involved in the transport of Fe from xylem to phloem, in loading Fe into phloem, and in the transmission of shoot-to-root iron signaling. Yet, apart from Arabidopsis, little is known about these transporters'functions in other plant species. OBJECTIVE:OPT3 proteins of several plant species were ch...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200625202028
更新日期:2020-06-25 00:00:00
abstract::Successful approaches of de novo protein design suggest a great potential to create novel structural folds and to understand natural rules of protein folding. For these purposes, smaller and simpler de novo proteins have been developed. Here, we constructed smaller proteins by removing the terminal sequences from stab...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612800494101
更新日期:2012-06-01 00:00:00
abstract::Polymyositis is an inflammatory myopathy characterized by muscle invasion of T-cells penetrating the basal lamina and displacing the plasma membrane of normal muscle fibers. In order to understand the different adhesive mechanisms at the T-cell surface, Schubert randomly selected 19 proteins expressed at the T-cell su...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612801619534
更新日期:2012-08-01 00:00:00
abstract::We report the physical-chemical characterization of the major alcohol-soluble proteins present in seeds of pearl millet (Pennisetum glaucum) by SDS-PAGE, bidimensional gel electrophoresis, MALDI-TOF/MS and RP-HPLC. We demonstrate the presence of three major prolamins, called A-, B- and C-pennisetin with mass values ar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408724
更新日期:2002-06-01 00:00:00
abstract::Antimicrobial peptides (AMPs) constitute an important alternative in the search for new treatments against pathogens. We analyzed the sequence variability in cytokine and chemokine proteins to investigate whether these molecules contain a sequence useful in the development of new AMPs. Cluster analysis allowed the ide...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866511320050001
更新日期:2013-05-01 00:00:00
abstract::Human butyrylcholinesterase (BuChE) is a serine enzyme present in most organs and plasma. No clear physiological function has yet been assigned to BuChE, but it is a pharmacologically and toxicologically important enzyme that plays a role in degradation of numerous ester-containing drugs and poisonous esters. Thus, Bu...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986609789071207
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:The O. tesota lectin PF2 is a tetrameric protein with subunits of 33 kDa that recognizes only complex carbohydrates, resistant to proteolytic enzymes and has insecticidal activity against Phaseolus beans pest. OBJECTIVE:To explore PF2 lectin features at different protein structural levels and to evaluate th...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200813204303
更新日期:2020-08-13 00:00:00
abstract::A missense mutation of Aspartic to Asparagine acid in 113 position of liver-targeting peptide CSP I-plus modified rEndostatin (rES-CSP) happened unexpectedly results in the changes of protein secondary structure and a reduced bioactivity. With the aim to clarify the structure-function relationships featuring the fuse ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866522666150302125218
更新日期:2015-01-01 00:00:00
abstract::Coiled coils are important structural modules and domains of protein-protein interactions. Further, they provide the required framework to proteins, like kinesins, myosins and SNAREs, which are either structural or involved in transport of biomolecules. We provide an interactive webserver to measure the strength of in...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608783330314
更新日期:2008-01-01 00:00:00
abstract::In this paper, a 3D map of protein fold space was produced using Dali structure alignment and nonmetric multidimensional scaling. The fold space comprises four radial clusters, which correspond to the four classes of SCOP. The overall structure of the protein fold space is largely determined by three factors: secondar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866054395257
更新日期:2005-07-01 00:00:00
abstract::The physiological stability curve--a plot of the free energy of unfolding versus temperature--is calculated for hen egg white lysozyme from a combination of extrapolated unfolding thermodynamic data from reversible conditions and isothermal titrations with guanidine hydrochloride. The shape of the curve suggests the e...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777841163
更新日期:2006-01-01 00:00:00
abstract::Loxosceles intermedia spider venom was subjected to proteomic analysis through a MudPIT shot-gun approach to identify the protein composition. Were identified 39 proteins which seem to responsible by the lesion of different types of tissues, to some physiopathological actions and by the prevention of structural damage...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609788923383
更新日期:2009-01-01 00:00:00
abstract::PepX is a X-prolyl dipeptidyl aminopeptidase of S15 family that cleaves dipeptides from the N-terminus of polypeptides having a proline or alanine residue at the second position. Involved in bacterial nutrition and in peptide maturation, this serine exopeptidase, counterpart of the mammalian DDP-4, has been proposed t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::The present study dealt with the isolation, identification and characterization of pigments from red snow samples of the Quito coastal front glacier (S 62º 27,217', W 059º 45,960') in Greenwich, Archipelago South Shetland, Antarctica, during summer 2013. As a strain of Shewanella was found to be the most common and ab...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866522666150915122247
更新日期:2015-01-01 00:00:00
abstract::The outcome of protein crystallization attempts is often uncertain due to inherent features of the protein or to the crystallization process that are not fully under control of the experimentalist. The aim of this contribution is to propose user-friendly tools that can increase the success rate of a protein crytalliza...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986612800793127
更新日期:2012-07-01 00:00:00
abstract::Bromelain inhibitor, "bromein", is a proteinase-inhibitor specific to the cysteine proteinase bromelain from pineapple stem. In the stem, eight bromein isoforms are known to exist, and each isoform has a short peptide (light chain) and a long one (heavy chain) with five disulfide bonds. The three-dimensional structure...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866525666180821115432
更新日期:2018-01-01 00:00:00
abstract::We previously described a potent anthrax toxin inhibitor, based on a phage-library-selected peptide sequence, synthesized as a tetra-branched molecule on a lysine core and further modified for improvement of activity [Pini et al., Biochem. J., 2006, 395, 157]. This branched peptide had very low solubility because of s...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608784966958
更新日期:2008-01-01 00:00:00
abstract::Plants reproductive phase, when grain yield and consequently farmers' investment is most in jeopardy, is considered as the most sensitive stage to drought stress. In this study, we aimed to explore the proteomic response of wheat anther at meiosis stage in a drought tolerant, Darab, and susceptible, Shiraz, wheat geno...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666161130143446
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Cryptochrome is a flavin-binding blue-light photoreceptor that functions in growth and development in plants, the circadian clock in animals and navigation in birds. However, a lack of purified cryptochrome has hindered studies of the structure and function of this protein. In this study, we obtained a subst...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666181004112308
更新日期:2018-01-01 00:00:00
abstract::Human ankyrin repeat and suppressor of cytokine signaling box protein 9 (hASB9), a subunit of an Elongin C-cullin-SOCS box (ECS) E3 ubiquitin ligase complex, is believed to be involved in specific substrate-recognition for ubiquitination and degradation. In fact, this specific substrate-recognition is determined by th...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609787601688
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Chickpea is a widely grown legume in India, Australia, Canada, and Mediterranean regions. Seeds of chickpea are good source of protein for both human and animals. Wild relatives of chickpea (Cicer arietinum) are the potential gene pool for crop improvement; however, very little information is available on th...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200910164118
更新日期:2020-09-10 00:00:00
abstract::Though protein folding is a regular phenomenon inside the cellular milieu, slight differences in the folding pattern of proteins may lead to disease-causing forms. Many diseases have been identified that are caused by these misfolded macromolecules and a considerable amount of focus is still directed towards better un...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866043406409
更新日期:2004-12-01 00:00:00
abstract::Background. Obesity is a major health problem in both developed and emerging countries. Obesity is a complex disease whose etiology involves genetic factors in strong interplay with environmental determinants and lifestyle. The discovery of genetic factors and biological pathways underlying human obesity is hampered b...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113209990053
更新日期:2014-01-01 00:00:00
abstract::The superoxide anion radical is a highly reactive toxic species produced during the metabolic processes. A number of copper (II) complexes with amino acids and peptides are known to show superoxide dismutase (SOD) like activity. The design and application of synthetic low molecular weight metal complexes as SOD mimics...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611797642724
更新日期:2011-12-01 00:00:00
abstract::Prediction of protein structure from its amino acid sequence is still a challenging problem. The complete physicochemical understanding of protein folding is essential for the accurate structure prediction. Knowledge of residue solvent accessibility gives useful insights into protein structure prediction and function ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612798472875
更新日期:2012-01-01 00:00:00
abstract::BmK ITa1 cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni(2+)-IDA-sepharose 6B column was toxic for insect, which indicated t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408472
更新日期:2002-10-01 00:00:00
abstract::Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-e...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866522666150428121442
更新日期:2015-01-01 00:00:00
abstract::Solid state nuclear magnetic resonance (NMR) has developed into one of the most informative and direct experimental approaches to the characterization of the molecular structures of amyloid fibrils, including those associated with Alzheimer's disease. In this article, essential aspects of solid state NMR methods are d...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986606775338470
更新日期:2006-01-01 00:00:00
abstract::Like those in mammals, heterotrimeric G protein complexes have been implicated in signal transduction pathways in plants; however, the subunits themselves have not been isolated. In this study, the rice heterotrimeric G protein subunits α (Gα) and β (Gβ) were purified by affinity chromatography using anti-Gα and -Gβ a...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612799363190
更新日期:2012-03-01 00:00:00
abstract::A halophilic nucleoside diphosphate kinase from a moderate halophile, Halomonas sp. 593 (593NDK), was found to be resistant to heat treatment, as indicated by the high level of activity recovery after heating at high temperatures. This is due to reversibility of thermal unfolding, not the high melting temperature, of ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606776819628
更新日期:2006-01-01 00:00:00