Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells.

abstract：:Classification of newly determined protein structures is important in understanding their function and mechanism of action. Currently available methods employ a global structure alignment strategy and are computationally expensive. We propose a two-step methodology with a quick screen to significantly reduce the numbe...

journal_title：Protein and peptide letters

authors： Dalal A,Deshmukh S,Wangika PP

更新日期：2007-01-01 00:00:00

abstract：:Aspergillus sp. phytase contains five disulfide bonds. In order to elucidate their role, the reactivation and refolding of phytase in the absence and presence of dithiothreitol (DTT) was investigated. The results indicated that the disulfide bonds play an important role in the catalytic activity and conformational sta...

journal_title：Protein and peptide letters

authors： Song GY,Wang XY,Wang M

更新日期：2005-08-01 00:00:00

abstract：:For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining (15)N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozy...

journal_title：Protein and peptide letters

authors： Nagata-Uchiyama M,Yaguchi M,Hirano Y,Ueda T

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Prmt5 plays major role in regulation of gene expression, RNA processing, cell growth and differentiation, signal transduction, germ cell development, etc., in mammals. Prmt5 is also related to cancer. Knowing the proteins interacting with Prmt5 is important to understand Prmt5's function in cells. Although t...

journal_title：Protein and peptide letters

authors： Shen H,Zhang X,Al Hafiz MA,Liang X,Yao Q,Guo M,Xu G,Zhong X,Zhou Q,Zhao H

更新日期：2020-01-01 00:00:00

abstract：:Cationic antimicrobial peptides (AMPs) are among the best studied antimicrobial factors expressed in the respiratory tract. AMPs are released by epithelial cells and immune cells into the airway surface liquid covering the epithelial surfaces of the lung where they act as endogenous antibiotics. Plenty of studies show...

journal_title：Protein and peptide letters

authors： Seiler F,Lepper PM,Bals R,Beisswenger C

更新日期：2014-04-01 00:00:00

abstract：:To identify proteins involved in the molecular mechanisms of pressure overload-induced heart failure, we quantitatively analyzed the left ventricular proteomic profile in a transverse aortic constriction (TAC) mouse model, a well-established model of cardiac hypertrophy and dysfunction. Multidimensional LC-MS/MS in co...

journal_title：Protein and peptide letters

authors： Guan D,Zhao Y,Zhang Y,Tang D,Wu Q

更新日期：2016-01-01 00:00:00

abstract：:Kallikrein-related peptidases (KLKs) are trypsin-like and chymotrypsin-like serine proteases which are expressed in several tissues. Their activity is tightly controlled by inhibitors including members of the serine protease Kazal-type (SPINK) family. These enzymes are promising targets for the treatment of skin desqu...

journal_title：Protein and peptide letters

authors： Assis DM,Zalazar L,Juliano MA,De Castro R,Cesari A

更新日期：2013-10-01 00:00:00

abstract：:Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and ...

journal_title：Protein and peptide letters

authors： Wu X,Li X,Su Z,Zheng Q,Xu H,Wu S,Feng Y,Zhao W

更新日期：2005-10-01 00:00:00

abstract：:In recent years, therapeutic proteins have become an important growing class of drugs in the pharmaceutics industry. The development of recombinant DNA technology has caused to appreciation of therapeutic value of many proteins and peptides in medicine. Currently, approximately 100 therapeutic proteins obtained approv...

journal_title：Protein and peptide letters

authors： Ozgur A,Tutar Y

更新日期：2013-12-01 00:00:00

abstract：:The mutation, conferring streptomycin and deoxyglucose resistance on cells, had profound effect on the kinetic and thermodynamic parameters inferring thermostabilization of beta-glucosidase from mutant 51 SM(r) of Cellulomonas biazotea. Free energy of activation for substrate binding, enthalpy and entropy of activatio...

journal_title：Protein and peptide letters

authors： Rajoka MI,Durrani IS,Khalid AM

更新日期：2005-04-01 00:00:00

abstract：:The human gut microbial ecosystem (HGME) exerts an important influence on the human health. In recent researches, meta-genomics provided deep insights into the HGME in terms of gene contents, metabolic processes and genome constitutions of meta-genome. Here we present a novel methodology to investigate the HGME on the...

journal_title：Protein and peptide letters

authors： Ji Y,Shi Y,Wang C,Dai J,Li Y

更新日期：2013-03-01 00:00:00

abstract：:An alternative method for expressing the recombinant proteins in Escherichia coli is proposed. Unlike the ordinary induction protocol where the cells in the early- to mid-log phase are induced for the protein production, this alternative protocol utilizes the cells in the stationary phase. By using a glutathione S-tra...

journal_title：Protein and peptide letters

authors： Chae YK,Cho KS,Chun W,Lee K

更新日期：2003-08-01 00:00:00

abstract：:Sry-related box (Sox) transcription factors share a conserved high-mobility-group box domain (HMG-domain) that binds DNA in the minor groove and bends DNA for further assembly of transcriptional machineries. During organogenesis, each member of the Sox family triggers a specific cell lineage differentiation, indicatin...

journal_title：Protein and peptide letters

authors： Gao N,Jiang W,Gao H,Cheng Z,Qian H,Si S,Xie Y

更新日期：2013-04-01 00:00:00

abstract：:Calreticulin is a soluble endoplasmic reticulum chaperone, which has a relatively low melting point due to its remarkable structure with a relatively high content of flexible structural elements. Using far ultraviolet circular dichroism (CD) spectroscopy and a fluorescent dye binding thermal shift assay, we have inves...

journal_title：Protein and peptide letters

authors： Duus K,Larsen N,Tran TA,Güven E,Skov LK,Jespersgaard C,Gajhede M,Houen G

更新日期：2013-05-01 00:00:00

abstract：:The importance of the intestinal microbiota of animals is widely acknowledged because of its vital role in the health of animals. There are complex communities of microbiota, which colonize the gastrointestinal tract. Intestinal microbiota are conductive to animal health and the development of the host immune system. ...

journal_title：Protein and peptide letters

authors： Hu S,Wang L,Jiang Z

更新日期：2017-01-01 00:00:00

abstract：:PepX is a X-prolyl dipeptidyl aminopeptidase of S15 family that cleaves dipeptides from the N-terminus of polypeptides having a proline or alanine residue at the second position. Involved in bacterial nutrition and in peptide maturation, this serine exopeptidase, counterpart of the mammalian DDP-4, has been proposed t...

journal_title：Protein and peptide letters

authors： André J,Bach M,Xie J,Rigolet P

更新日期：2013-01-01 00:00:00

abstract：:Two cell-penetrating peptides, a Pro-rich peptide derivative, acetyl-(Val-Arg-Leu-Pro-Pro-Pro)(3)-Gly-Cys amide, and an octaarginine derivative, acetyl-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys amide, were prepared by the solid phase method. Each peptide was coupled to the heterobifunctional cross-linking reagent, 6-mal...

journal_title：Protein and peptide letters

authors： Kida S,Eto Y,Yoshioka Y,Nakagawa S,Kawasaki K,Maeda M

更新日期：2010-02-01 00:00:00

abstract：:Identification and elimination of noise peaks in mass spectra from large proteomics data streams simultaneously improves the accuracy of peptide identification and significantly decreases the size of the data. There are a number of peak filtering strategies that can achieve this goal. Here we present a simple algorith...

journal_title：Protein and peptide letters

authors： Reiz B,Myers MP,Pongor S,Kertesz-Farkas A

更新日期：2014-01-01 00:00:00

abstract：:We report a new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed a truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and eval...

journal_title：Protein and peptide letters

authors： Wang L,Xu D,Qian M,Yang G,Wu X

更新日期：2006-01-01 00:00:00

abstract：:In 2008 cancer was identified by the World Health Organization (WHO) as one of four threats to human health and development. Since the early published reports of the first chemotherapeutic, mustine, in 1946, the anti-cancer drug and development industry has grown into a multi-billion dollar business enterprise. Worldw...

journal_title：Protein and peptide letters

authors： Li Q,Yi L,Marek P

更新日期：2014-01-01 00:00:00

abstract：:The immunogenicity of "novel" MART-1 and Tyrosinase class-II peptides was assessed in transgenic mice. Tyrosinase(141-161) peptide was found to be immunogenic and endogenously processed in the HLA-DRbeta1*0101 and HLA-DRbeta1*0401 transgenic mice with peptide specific production of IFNgamma or IL-5 respectively. The M...

journal_title：Protein and peptide letters

authors： Horton RB,Laversin SA,Reeder SP,Rees RC,McArdle SE

更新日期：2007-01-01 00:00:00

abstract：:Identification on protein folding types is always based on the 27-class folds dataset, which was provided by Ding & Dubchak in 2001. But with the avalanche of protein sequences, fold data is also expanding, so it will be the inevitable trend to improve the existing dataset and expand more folding types. In this paper,...

journal_title：Protein and peptide letters

authors： Liu L,Hu XZ,Liu XX,Wang Y,Li SB

更新日期：2012-04-01 00:00:00

abstract：:AB204 is an Activin/BMP2 chimera, which has been found to exhibit a higher activity than Bone Morphogenetic Protein 2 (BMP2) in osteogenic activity. To prepare AB204 for its preclinical studies, AB204 has been characterized in various formulation buffers. We observed that AB204 purified by ion-exchange chromatography ...

journal_title：Protein and peptide letters

authors： Ahn C,Maslennikov I,Choi JY,Oh H,Cheong C,Choe S

更新日期：2014-05-01 00:00:00

abstract：BACKGROUND:It is currently believed that protein folding rates are influenced by protein structure, environment and temperature, amino acid sequence and so on. We have been working for long to determine whether and in what ways mRNA affects the protein folding rate. A large number of palindromes aroused our attention i...

journal_title：Protein and peptide letters

authors： Li R,Li H,Yang S,Feng X

更新日期：2020-01-01 00:00:00

abstract：:Bucain, a potent neurotoxin isolated from the venom of the Malayan krait (Bungarus candidus), induces paralysis and death. Its crystal structure has been determined at 2.10 A resolution and based on the molecular topology and hydrophobicity profile is structurally classified as a three-fingered alpha-neurotoxin posses...

journal_title：Protein and peptide letters

authors： Murakami MT,Kini RM,Arni RK

更新日期：2009-01-01 00:00:00

abstract：:Protein phosphatase magnesium-dependent 1, delta (PPM1D) is a member of the PPM1 (formerly PP2C) protein phosphatase family, and is induced in response to DNA damage. The overexpression of PPM1D is thought to exert oncogenic effects through the inhibition of tumor suppressor proteins. PPM1D shows high selectivity for ...

journal_title：Protein and peptide letters

authors： Chuman Y,Yagi H,Fukuda T,Nomura T,Matsukizono M,Shimohigashi Y,Sakaguchi K

更新日期：2008-01-01 00:00:00

abstract：:Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...

journal_title：Protein and peptide letters

authors： Zhang XC,Wang WD,Wang JS,Pan JC,Zou GL

更新日期：2011-12-01 00:00:00

abstract：:The role of interaction between Asn259 (catalytic domain) with Gln821 (C-terminal domain) in PeptidaseN was investigated. The k(cat) of PeptidaseN containing Asn259Asp or Gln821Glu is enhanced whereas it is suppressed in Asn259AspGln821Glu. Structural analysis shows this interaction to change the relative disposition ...

journal_title：Protein and peptide letters

authors： Kumar A,Reddy S,Srinivasan N,Nandi D

更新日期：2009-01-01 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is known to interact with different biomolecules and was implicated in many novel cellular activities including programmed cell death, nuclear RNA transport unrelated to the commonly known carbohydrate metabolism. We reported here the purification of GAPDH from Chironom...

journal_title：Protein and peptide letters

authors： Chong IK,Ho WS

更新日期：2013-09-01 00:00:00

abstract：BACKGROUND:Bursa of Fabricius plays the vital functions on B cell development and antibody production in poultry. The bursal-derived peptide plays the essential roles on avian immature B cell development. OBJECTIVES:Here we explored the functions of the recently reported bursal nonapeptide (BP9) on the antibody produc...

journal_title：Protein and peptide letters

authors： Zheng Y,Zong MM,Chen BY,Zhou XH,Liu ZN,Zhou GF,Chen PY,Feng XL

更新日期：2019-01-01 00:00:00