Abstract:
:Most proteinaceous pores are characterized as ionic channels. However, some are also involved in protein translocation through phospholipidic membranes. This concept has evolved slowly in cell biology and in biophysics, requiring the development of adapted electrical and biochemical methods. Protein translocation in mitochondria biogenesis, secretion by endoplasmic reticulum or bacteria, and bacterial toxins internalization are the main fields where proteinconducting pores have been described. The concept is now well established and progress at the molecular and atomic levels have shown how different this paradigm is from ionic channels involved in neurobiology. Protein-conducting pores are often parts of large complexes and electrical analysis gives on-line information at the single-molecule level. They have a large conductance that, in certain membranes, should be highly regulated to prevent ionic leaking through the membrane. Finally, they are involved not only in protein translocation, but also in membrane protein insertion (α-helix and β-barrel types).
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Henry JPdoi
10.2174/09298665113209990076subject
Has Abstractpub_date
2014-03-01 00:00:00pages
202-8issue
3eissn
0929-8665issn
1875-5305pii
PPL-EPUB-58331journal_volume
21pub_type
杂志文章,评审abstract::To identify proteins involved in the molecular mechanisms of pressure overload-induced heart failure, we quantitatively analyzed the left ventricular proteomic profile in a transverse aortic constriction (TAC) mouse model, a well-established model of cardiac hypertrophy and dysfunction. Multidimensional LC-MS/MS in co...
journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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abstract::Like those in mammals, heterotrimeric G protein complexes have been implicated in signal transduction pathways in plants; however, the subunits themselves have not been isolated. In this study, the rice heterotrimeric G protein subunits α (Gα) and β (Gβ) were purified by affinity chromatography using anti-Gα and -Gβ a...
journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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abstract::To investigate the function of plant plasma membrane, proteins of rice plasma membrane were analyzed and the proteins changed by cold stress were identified. Plasma membrane proteins were purified with an aqueous two-phase partitioning method from root of rice seedlings, and activity of specific H(+)-ATPase localized ...
journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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