Abstract:
BACKGROUND:NMAAP1 plays a role in regulating macrophage differentiation to the M1 type and exerting antitumoral functions. It is not clear what role and mechanism NMAAP1 does play in the reversal of macrophages from M1 to M2. METHODS:We detected the typing of macrophages with high or low expression of NMAAP1 by QPCR and ELISA, and detected the colocalization of NMAAP1 and endogenous IP3R by laser confocal microscopy, and detected the protein expression in cells by Western-blotting. RESULTS:Our study found that knockdown NMAAP1 in RAW264.7 cells induced macrophage polarization to the M2 type and up-regulation of NMAAP1 in RAW264.7 cells maintain M1 Phenotype even in the presence of IL-4, a stronger inducer of the M2 type. Additionally, Coimmunoprecipitation revealed a protein-protein interaction between NMAAP1 and IP3R and then activates key molecules in the PKC-dependent Raf/MEK/ERK and Ca2+/CaM/CaMKII signaling pathways. Activation of PKC (Thr638/641), ERK1/2 (Thr202/Tyr204) and CaMKII (Thr286) is involved in the regulation of cell differentiation. CONCLUSION:NMAAP1 interacts with IP3R, which in turn activates the PKC-dependent Raf/MEK/ERK and Ca2+/CaM/CaMKII signaling pathways. These results provide a new explanation of the mechanism underlying M1 differentiation.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Liu Q,Zhu P,Liu S,Tang M,Wang Y,Tian Y,Jin Z,Li D,Yan Ddoi
10.2174/0929866526666190503105343subject
Has Abstractpub_date
2019-01-01 00:00:00pages
751-757issue
10eissn
0929-8665issn
1875-5305pii
PPL-EPUB-98346journal_volume
26pub_type
杂志文章abstract::DNA fragmentation factor 45 (DFF45) regulates DNase DFF40 as its inhibitor and chaperone. It was reported that the N-terminal domain (NTD) of DFF45 alone is disordered and DFF40 is necessary as a mutual chaperone for the folding of NTD. However, here we reported the crystallization of DFF45 NTD. These crystals diffrac...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866033479068
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abstract::After consideration of the tertiary structure of the pY+3 binding pocket in SH2 domains, isoleucine of Ac-pYEEIE was replaced with various unnatural aliphatic amino acids and the binding affinities for Lck, Src, and Fyn SH2 domains were measured. We determined the characteristics of the amino acid at the pY+3 position...
journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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abstract::Sexual maturation and olfactory bulb defects found in prokineticin 2 (Pk2) and prokineticin receptor 2 (Pkr2) mutant mice resembling the phenotypic characteristics of Kallmann syndrome (KS), gave rise to the question of whether these genes would have a role in KS pathogenesis. Later, mutations in both genes were ident...
journal_title:Protein and peptide letters
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更新日期:2016-01-01 00:00:00
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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更新日期:2009-01-01 00:00:00
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journal_title:Protein and peptide letters
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更新日期:2009-01-01 00:00:00
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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abstract::Behcet's disease (BD) is a recurrent pathema with a typical symptom of inflammation involved in many organs. Previous report indicated that the serum of Korean patients with BD stimulates membrane expression of hnRNP A2/B1 in endothelial cells. In this study, the target 35 kDa recombinant human hnRNP A2/B1 were over-e...
journal_title:Protein and peptide letters
pub_type: 杂志文章
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journal_title:Protein and peptide letters
pub_type: 杂志文章
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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更新日期:2012-08-01 00:00:00
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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更新日期:2010-02-01 00:00:00
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journal_title:Protein and peptide letters
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更新日期:2018-01-01 00:00:00
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journal_title:Protein and peptide letters
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