Drugs against Mycobacterium tuberculosis 3-isopropylmalate dehydrogenase can be developed using homologous enzymes as surrogate targets.

abstract：BACKGROUND:Cathepsin D is a lysosomal enzyme that is found in all organisms acting in protein turnover, in humans it is present in some types of carcinomas, and it has a high activity in Parkinson's disease and a low activity in Alzheimer disease. In marine organisms, most of the research has been limited to corroborat...

journal_title：Protein and peptide letters

authors： Francisco CC,Luis CJ,Marina EJ,Javier CF,Alexis LA,Del Carmen SH,Alfredo RI

更新日期：2019-01-01 00:00:00

abstract：:The studies presented here explore antimicrobial peptide preferential binding behavior for a target pathogen, Escherichia coli O157:H7. A modified immunoassay and surface plasmon resonance were employed to evaluate immobilized peptide binding of whole bacterial cells. The knowledge gained may guide the rational design...

journal_title：Protein and peptide letters

authors： Soares JW,Kirby R,Morin KM,Mello CM

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Reversibly glycosylated polypeptide (RGP), a kind of hydrosoluble and plasmodesmal-associated protein found in plants, plays a crucial role in the development of pollen. OBJECTIVE:A novel RGP 2 was isolated and identified from rape (Brassica napus L.) bee pollen. METHOD:RGP2 was isolated and purified by io...

journal_title：Protein and peptide letters

authors： Zhang Q,Sun T,Tuo X,Li Y,Yang H,Deng J

更新日期：2020-11-03 00:00:00

abstract：:Nucleobindin-2 is a 420 amino acid EF-hand Ca²⁺ binding protein that can be further processed to generate an 82 amino terminal peptide termed Nesfatin-1. To examine the function of secreted Nucleobindin-2 in adipocyte differentiation, cultured 3T3-L1 cells were incubated with either 0 or 100 nM of GST, GST-Nucleobindi...

journal_title：Protein and peptide letters

authors： Tagaya Y,Osaki A,Miura A,Okada S,Ohshima K,Hashimoto K,Yamada M,Satoh T,Shimizu H,Mori M

更新日期：2012-09-01 00:00:00

abstract：:Heparin is naturally occurring polysaccharides which interacts with seminal plasma proteins and regulate multiple steps in fertilization process. Qualitative and quantitative information regarding the affinity for heparin-seminal plasma proteins interactions is not generally well documented and there are no reports of...

journal_title：Protein and peptide letters

authors： Kumar V,Yadav VK,Hassan MI,Singh AK,Dey S,Singh S,Singh TP,Yadav S

更新日期：2012-08-01 00:00:00

abstract：:Gibberellins (GAs) are tetracyclic, diterpenoid plant hormones, essential for many developmental processes in higher plants. Plants perceive GA through a nuclear-localized GA receptor, GA INSENSITIVE DWARF1 (GID1). From sequence similarity, it is suggested that GID1 evolved from a hormone-sensitive lipase (HSL), and r...

journal_title：Protein and peptide letters

authors： Hirano K,Aya K,Matsuoka M,Ueguchi-Tanaka M

更新日期：2012-02-01 00:00:00

abstract：BACKGROUND:It is currently believed that protein folding rates are influenced by protein structure, environment and temperature, amino acid sequence and so on. We have been working for long to determine whether and in what ways mRNA affects the protein folding rate. A large number of palindromes aroused our attention i...

journal_title：Protein and peptide letters

authors： Li R,Li H,Yang S,Feng X

更新日期：2020-01-01 00:00:00

abstract：:The description of protein backbone was recently improved with a group of structural fragments called Structural Alphabets instead of the regular three states (Helix, Sheet and Coil) secondary structure description. Protein Blocks is one of the Structural Alphabets used to describe each and every region of protein bac...

journal_title：Protein and peptide letters

authors： Suresh V,Ganesan K,Parthasarathy S

更新日期：2013-03-01 00:00:00

abstract：:A proof-of-principle methodology is presented in which all commercially-available cysteine (Cys) and selenocysteine (Sec) solid phase peptide synthesis (SPPS) derivatives are synthesized in high yield from easily prepared protected dichalcogenide precursors. A Zn-mediated biphasic reduction process applied to a series...

journal_title：Protein and peptide letters

authors： Flemer S

更新日期：2014-01-01 00:00:00

abstract：:Arginine kinase is an essential enzyme which is closely related to energy metabolism in marine invertebrates. Arginine kinase provides a significant role in quick response to environmental change and stress. In this study, we simulated a tertiary structure of Sepia pharaonis arginine kinase (SPAK) based on the gene se...

journal_title：Protein and peptide letters

authors： Si YX,Lee J,Cheng JG,Yin SJ,Park YD,Qian GY,Jiang XM

更新日期：2016-01-01 00:00:00

abstract：:Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...

journal_title：Protein and peptide letters

authors： Zhang XC,Wang WD,Wang JS,Pan JC,Zou GL

更新日期：2011-12-01 00:00:00

abstract：:Protein structure information is very useful for the confirmation of protein function. The protein structural class can provide information for protein 3D structure analysis, causing the conformation of the protein overall folding type plays a significant part in molecular biology. In this paper, we focus on the predi...

journal_title：Protein and peptide letters

authors： Liao B,Xiang Q,Li D

更新日期：2012-11-01 00:00:00

abstract：:α-Synuclein forms amyloid deposits in the dopaminergic neurons; a process that is believed to contribute to the Parkinson's disease. An emerging theme in amyloid research is the hypothesis that the toxic species produced during amyloid formation share common physic-chemical features and exert their effects by common m...

journal_title：Protein and peptide letters

authors： Ruzza P,Gazziero M,Marchi MD,Massalongo G,Marchiani A,Autiero I,Tessari I,Bubacco L,Calderan A

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Haloalkane dehalogenases (EC 3.8.1.5, HLDs) are α/β-hydrolases which catalyze the irreversible cleavage of carbon-halogen bonds of haloalkanes, producing an alcohol, a halide and a hydrogen ion. Haloalkanes are acutely toxic to animals and humans and their toxic effects are mainly observed in the liver, kidn...

journal_title：Protein and peptide letters

authors： Georgakis N,Chronopoulou E,Gad MA,Skliros D,Efrose R,Flemetakis E,Labrou NE

更新日期：2017-01-01 00:00:00

abstract：:The self-assembly of short peptide building blocks into well-ordered nanostructures is a key direction in bionanotechnology. The formation of β -sheet organizations by short peptides is well explored, leading to the development of a wide range of functional assemblies. Likewise, many natural proteinaceous materials, s...

journal_title：Protein and peptide letters

authors： Bera S,Gazit E

更新日期：2019-01-01 00:00:00

abstract：:Nicotinamide adenine dinucleotide (NAD) plays an important role in cellular metabolism and acts as hydrideaccepting and hydride-donating coenzymes in energy production. Identification of NAD protein interacting sites can significantly aid in understanding the NAD dependent metabolism and pathways, and it could further...

journal_title：Protein and peptide letters

authors： Wang X,Wang CC,Zhang YQ,Mi G,Zhang J,Li ML

更新日期：2012-05-01 00:00:00

abstract：:The mutation, conferring streptomycin and deoxyglucose resistance on cells, had profound effect on the kinetic and thermodynamic parameters inferring thermostabilization of beta-glucosidase from mutant 51 SM(r) of Cellulomonas biazotea. Free energy of activation for substrate binding, enthalpy and entropy of activatio...

journal_title：Protein and peptide letters

authors： Rajoka MI,Durrani IS,Khalid AM

更新日期：2005-04-01 00:00:00

abstract：:Though protein folding is a regular phenomenon inside the cellular milieu, slight differences in the folding pattern of proteins may lead to disease-causing forms. Many diseases have been identified that are caused by these misfolded macromolecules and a considerable amount of focus is still directed towards better un...

journal_title：Protein and peptide letters

authors： Nayeem MS,Khan RH

更新日期：2004-12-01 00:00:00

abstract：:Identification on protein folding types is always based on the 27-class folds dataset, which was provided by Ding & Dubchak in 2001. But with the avalanche of protein sequences, fold data is also expanding, so it will be the inevitable trend to improve the existing dataset and expand more folding types. In this paper,...

journal_title：Protein and peptide letters

authors： Liu L,Hu XZ,Liu XX,Wang Y,Li SB

更新日期：2012-04-01 00:00:00

abstract：:Catalase is an enzyme that occurs in almost all aerobic organisms. Its main metabolic function is to prevent oxidative damage to tissues induced by hydrogen peroxide which is a strong oxidizing agent. Catalase is very effective in performing this task, since it has the highest turnover rate among all the enzymes. The ...

journal_title：Protein and peptide letters

authors： Seixas FA,da Silva MR,Murakami MT,Tosqui P,Colombo MF

更新日期：2011-09-01 00:00:00

abstract：:We previously described a potent anthrax toxin inhibitor, based on a phage-library-selected peptide sequence, synthesized as a tetra-branched molecule on a lysine core and further modified for improvement of activity [Pini et al., Biochem. J., 2006, 395, 157]. This branched peptide had very low solubility because of s...

journal_title：Protein and peptide letters

authors： Pini A,Brunetti J,Falciani C,Fabbrini M,Bracci L

更新日期：2008-01-01 00:00:00

abstract：:After several studies on prediction of mutation, we examine the effect of three sampling strategies, the sampling based on years, the sampling based on number of mutations, and the sampling based on the unpredictable portion of amino-acid pairs, on the prediction performance in H5N1 hemagglutinins. The results show th...

journal_title：Protein and peptide letters

authors： Wu G,Yan S

更新日期：2008-01-01 00:00:00

abstract：:The importance of the intestinal microbiota of animals is widely acknowledged because of its vital role in the health of animals. There are complex communities of microbiota, which colonize the gastrointestinal tract. Intestinal microbiota are conductive to animal health and the development of the host immune system. ...

journal_title：Protein and peptide letters

authors： Hu S,Wang L,Jiang Z

更新日期：2017-01-01 00:00:00

abstract：:The outcome of protein crystallization attempts is often uncertain due to inherent features of the protein or to the crystallization process that are not fully under control of the experimentalist. The aim of this contribution is to propose user-friendly tools that can increase the success rate of a protein crytalliza...

journal_title：Protein and peptide letters

authors： Sánchez-Puig N,Sauter C,Lorber B,Giegé R,Moreno A

更新日期：2012-07-01 00:00:00

abstract：:The present paper reviews the use of expressed protein ligation for the biosynthesis of backbone cyclized polypeptides. This general method allows the in vivo and in vitro biosynthesis of cyclic polypeptides using recombinant DNA expression techniques. Biosynthetic access to backbone cyclic peptides opens the possibil...

journal_title：Protein and peptide letters

authors： Kimura R,Camarero JA

更新日期：2005-11-01 00:00:00

abstract：:DNA fragmentation factor 45 (DFF45) regulates DNase DFF40 as its inhibitor and chaperone. It was reported that the N-terminal domain (NTD) of DFF45 alone is disordered and DFF40 is necessary as a mutual chaperone for the folding of NTD. However, here we reported the crystallization of DFF45 NTD. These crystals diffrac...

journal_title：Protein and peptide letters

authors： Li T,Li X,Yang W,Rao Z,Zhai Zh

更新日期：2003-04-01 00:00:00

abstract：:The present study dealt with the isolation, identification and characterization of pigments from red snow samples of the Quito coastal front glacier (S 62º 27,217', W 059º 45,960') in Greenwich, Archipelago South Shetland, Antarctica, during summer 2013. As a strain of Shewanella was found to be the most common and ab...

journal_title：Protein and peptide letters

authors： Martín-Cerezo ML,García-López E,Cid C

更新日期：2015-01-01 00:00:00

abstract：:The role of interaction between Asn259 (catalytic domain) with Gln821 (C-terminal domain) in PeptidaseN was investigated. The k(cat) of PeptidaseN containing Asn259Asp or Gln821Glu is enhanced whereas it is suppressed in Asn259AspGln821Glu. Structural analysis shows this interaction to change the relative disposition ...

journal_title：Protein and peptide letters

authors： Kumar A,Reddy S,Srinivasan N,Nandi D

更新日期：2009-01-01 00:00:00

abstract：:The small heat shock protein SsHSP14.1 from the hyper-thermophilic archeaon, Sulfolobus solfataricus (S. solfataricus) was able to protect proteins from thermal aggregation and prevent enzymes from heat induced inactivation. According to the 3D (dimensional) structural model of SsHSP14.1 developed by us before, the re...

journal_title：Protein and peptide letters

authors： Wen ZZ,Wang YH,Yang B,Xie MQ,Chou KC

更新日期：2011-03-01 00:00:00

abstract：:Thioredoxin (TRX) catalyzes redox reactions via the reversible oxidation of the conserved active center CGPC and it is involved in multiple biological processes, some of them linked to redox activity while others not. TRX is a globular, thermodynamically stable and monomeric alpha/beta protein with a structure charact...

journal_title：Protein and peptide letters

authors： Vazquez DS,Delfino JM,Santos J

更新日期：2015-01-01 00:00:00