Kinetics for Zinc Ion Induced Sepia Pharaonis Arginine Kinase Inactivation and Aggregation.

Abstract:

:Arginine kinase is an essential enzyme which is closely related to energy metabolism in marine invertebrates. Arginine kinase provides a significant role in quick response to environmental change and stress. In this study, we simulated a tertiary structure of Sepia pharaonis arginine kinase (SPAK) based on the gene sequence and conducted the molecular dynamics simulations between SPAK and Zn(2+). Using these results, the Zn(2+) binding sites were predicted and the initial effect of Zn(2+) on the SPAK structure was elucidated. Subsequently, the experimental kinetic results were compared with the simulation results. Zn(2+) markedly inhibited the activity of SPAK in a manner of non-competitive inhibitions for both arginine and ATP. We also found that Zn(2+) binding to SPAK resulted in tertiary conformational change accompanying with the hydrophobic residues exposure. These changes caused SPAK aggregation directly. We screened two protectants, glycine and proline, which effectively prevented SPAK aggregation and recovered the structure and activity. Overall, our study suggested the inhibitory effect of Zn(2+) on SPAK and Zn(2+) can trigger SPAK aggregation after exposing large extent of hydrophobic surface. The protective effects of glycine and proline against Zn(2+) on SPAK folding were also demonstrated.

journal_name

Protein Pept Lett

authors

Si YX,Lee J,Cheng JG,Yin SJ,Park YD,Qian GY,Jiang XM

doi

10.2174/0929866523666160331144049

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

508-17

issue

6

eissn

0929-8665

issn

1875-5305

pii

PPL-EPUB-74672

journal_volume

23

pub_type

杂志文章
  • Domestic arthropods and their allergens.

    abstract::Allergy prevalence has increased worldwide over the last 25 years along with industrialization and westernized lifestyles. Indoor allergens are primarily responsible for the sensitization and development of atopic diseases. The main indoor allergens are known to be derived from various arthropods which account for up ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986607782541114

    authors: Jeong KY,Hong CS,Yong TS

    更新日期:2007-01-01 00:00:00

  • Structural, Functional and Evolutionary Aspects of Seed Globulins.

    abstract::Globulins are a major class of seed storage proteins which were thought to be enzymatically inactive. These proteins belong to the most ancient cupin superfamily. They can be graded into 11S legumin type and 7S vicilin type based on their sedimentation coefficients. Members from both classes share structural homology ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/0929866523666161220112641

    authors: Kesari P,Neetu,Sharma A,Katiki M,Kumar P,Gurjar BR,Tomar S,Sharma AK,Kumar P

    更新日期:2017-01-01 00:00:00

  • Peptoid analogues of anoplin show antibacterial activity.

    abstract::We have synthesised nine analogues of the antibacterial peptide anoplin with a peptoid residue at position 5 (H-GLLKXIKTLL-NH(2)). The most active compounds showed MIC-values of 12.5 and 25 microM against E.coli and S.aureus. These MIC-values are comparable with anoplin which showed 23 microM and 11 microM against E. ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986609789055421

    authors: Meinike K,Hansen PR

    更新日期:2009-01-01 00:00:00

  • Sequence and structural perspectives of bacterial β-stranded porins.

    abstract::Porins are integral membrane proteins found in the outer membrane of bacteria, mitochondria and chloroplasts. Herein, we have reviewed sequence and structural understanding about bacterial porins. The first porin structure from Rhodobacter capsulatus at 1.8 Å resolution in 1991 till the recent structural advancement, ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866521666140827110755

    authors: Kumar A,Bhandari A,Krishnaswamy S

    更新日期:2015-01-01 00:00:00

  • In silico modulation of HMGN-1 binding to histones and gene expression by interplay of phosphorylation and O-GlcNAc modification.

    abstract::Utilizing different computational methods; phosphorylation, O-GlcNAc modification and Yin Yang sites are predicted in HMGN-1. Prediction results suggest that interplay of phosphorylation and O-GlcNAc modification regulates binding and removal of HMGN-1 with the nucleosome and its translocation from nucleus to cytoplas...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986608783489607

    authors: Ahmad I,Khan TS,Hoessli DC,Walker-Nasir E,Kaleem A,Shakoori AR,Nasir-ud-Din

    更新日期:2008-01-01 00:00:00

  • Histatins in non-human primates: gene variations and functional effects.

    abstract::Human histatins are histidine-rich, low molecular weight salivary proteins that contribute to the immune system of the oral cavity. In this work, nucleotide sequences of the HIS1 (coding for histatin 1) and HIS2 (coding for histatin 3) genes, homologous to the human ones, have been sequenced and analysed in five prima...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986610791306715

    authors: Padovan L,Segat L,Pontillo A,Antcheva N,Tossi A,Crovella S

    更新日期:2010-07-01 00:00:00

  • Visualization of protein fold space via nonmetric multidimensional scaling.

    abstract::In this paper, a 3D map of protein fold space was produced using Dali structure alignment and nonmetric multidimensional scaling. The fold space comprises four radial clusters, which correspond to the four classes of SCOP. The overall structure of the protein fold space is largely determined by three factors: secondar...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866054395257

    authors: Xu Q

    更新日期:2005-07-01 00:00:00

  • Gbetagamma-copurified lipid kinase impurity from Sf9 cells.

    abstract::G-protein betagamma dimers are prime regulators transmitting extracellular signals to wide-ranging cellular effectors including phosphoinositide 3-kinase (PI3K) isoforms beta and gamma. Recombinant Gbetagamma purified from Sf9 cells via metal-affinity and anion exchange chromatography exhibited a wortmannin-insensitiv...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986609789055340

    authors: Shymanets A,Ahmadian MR,Nürnberg B

    更新日期:2009-01-01 00:00:00

  • Identification of binding partners of CsaA - an archaeal chaperonic pro-tein of Picrophilus torridus.

    abstract:BACKGROUND:CsaA is among the few chaperones which are present in both bacteria and archaea, but absent in eukaryotes. There are no reports on interactome analysis of CsaA from archaea, till date. Identification of binding partners of CsaA might be helpful in understanding CsaA-associated processes in Picrophilus torrid...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866527999201126205131

    authors: Singhal N,Sharma A,Aswal M,Singh N,Kumar M,Goel M

    更新日期:2020-11-26 00:00:00

  • Precursor mass dependent filtering of mass spectra for proteomics analysis.

    abstract::Identification and elimination of noise peaks in mass spectra from large proteomics data streams simultaneously improves the accuracy of peptide identification and significantly decreases the size of the data. There are a number of peak filtering strategies that can achieve this goal. Here we present a simple algorith...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/09298665113209990065

    authors: Reiz B,Myers MP,Pongor S,Kertesz-Farkas A

    更新日期:2014-01-01 00:00:00

  • Crystallization and preliminary crystallographic analysis of human eukaryotic translation initiation factor 5A (eIF-5A).

    abstract::Eukaryotic translation initiation factor 5A (eIF-5A) is universally found in all eukaryotic cells. It is the only protein in nature known to contain the unusual amino acid hypusine, a post-translationally modified lysine. Recombinant human eIF-5A was crystallized by the hanging-drop vapor diffusion method. Crystals we...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866054696091

    authors: Sun Y,Li X,Wu B,Sun P,Rao Z

    更新日期:2005-10-01 00:00:00

  • Computational Models for Self-Interacting Proteins Prediction.

    abstract::Self-Interacting Proteins (SIPs), whose two or more copies can interact with each other, have significant roles in cellular functions and evolution of Protein Interaction Networks (PINs). Knowing whether a protein can act on itself is important to understand its functions. Previous studies on SIPs have focused on thei...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/0929866527666191227141713

    authors: Qu J,Zhao Y,Zhang L,Cai SB,Ming Z,Wang CC

    更新日期:2020-01-01 00:00:00

  • Detailed folding structures of kappa-conotoxin RIIIJ and its mutageneses obtained from 2-dimensional HP model.

    abstract::Kappa-conotoxin RIIIJ is a conopeptide to inhibit voltage-gated potassium channels, however, its detailed folding structures have yet to be studied. With the advance in computing power, it is possible to use the HP model to analyze all its possible folding structures. In this study, the amino acid sequences of kappa-c...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986612800190982

    authors: Yan S,Wu G

    更新日期:2012-05-01 00:00:00

  • Intrinsic disorder in the core proteins of flaviviruses.

    abstract::Hepatitis C virus and related viruses in the Flaviviridae family (such as dengue virus, yellow fever virus or West Nile virus) are amongst the most important human pathogens, causing substantial morbidity and mortality world-wide. The production of viral progeny in Flaviviridae is orchestrated by the small, multifunct...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986610791498911

    authors: Ivanyi-Nagy R,Darlix JL

    更新日期:2010-08-01 00:00:00

  • Identifying protein complexes by reducing noise in interaction networks.

    abstract::Identifying protein complexes in protein-protein interaction (PPI) networks is a fundamental problem in computational biology. High-throughput experimental techniques have generated large, experimentally detected PPI datasets. These interactions represent a rich source of data that can be used to detect protein comple...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866521666140320111720

    authors: Liao B,Fu X,Cai L,Chen H

    更新日期:2014-07-01 00:00:00

  • Radical damage involving sulfur-containing enzymes and membrane lipids.

    abstract::Free radicals induce protein modifications, often associated to many biological phenomena. This mini-review overviews the approaches we have used to elucidate the radical-induced damages on sulfur-containing enzymes, such as ribonuclease and lysozyme, and the possible post-translational mechanism of the damage to anot...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986607781483840

    authors: Torreggiani A,Tamba M,Ferreri C

    更新日期:2007-01-01 00:00:00

  • Development and Characterization of Polymeric Peptides for Antibody Tagging of Bacterial Targets.

    abstract:BACKGROUND:Microbe-Binding Peptides (MBPs) are currently being investigated to address the problem of antimicrobial resistance. Strategies enhancing their antimicrobial activity have been developed, including peptide dimerization. Here, we present an alternative approach based on peptide polymerization, yielding hapten...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866527666200427212940

    authors: Climacosa FMM,King RAN,Santos BMM,Caoili SEC

    更新日期:2020-01-01 00:00:00

  • Purification and properties of laticeptin, an antimicrobial peptide from skin secretions of the South American frog Leptodactylus laticeps.

    abstract::Norepinephrine-stimulated skin secretions from the Sante Fe frog Leptodactylus laticeps contained high concentrations of a peptide, termed laticeptin, with the primary structure Gly-Val-Val-Asp-Ile-Leu-Lys-Gly-Ala-Ala-Lys-Asp-Leu-Ala-Gly-His-Leu-Ala-Thr-Lys-Val-Met-Asn-Lys-Leu.NH(2). Laticeptin inhibited the growth of...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986606775974410

    authors: Conlon JM,Al-Ghaferi N,Abraham B,Sonnevend A,King JD,Nielsen PF

    更新日期:2006-01-01 00:00:00

  • Biochemical characterization of a new maize (Zea mays L.) peptide growth factor.

    abstract::Coordination of cell growth and cell division is very important for living organisms in order for these to develop harmonically. The present research is concerned with the purification and characterization of a new peptide hormone, namely ZmIGF (Zea mays insulin-like growth factor), which regulates growth and cell div...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986611794328636

    authors: Rodríguez-López CD,Rodríguez-Romero A,Aguilar R,de Jiménez ES

    更新日期:2011-01-01 00:00:00

  • Effect of Temperature and pH on the Secondary Structure and Denaturation Process of Jumbo Squid Hepatopancreas Cathepsin D.

    abstract:BACKGROUND:Cathepsin D is a lysosomal enzyme that is found in all organisms acting in protein turnover, in humans it is present in some types of carcinomas, and it has a high activity in Parkinson's disease and a low activity in Alzheimer disease. In marine organisms, most of the research has been limited to corroborat...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866526666190405124353

    authors: Francisco CC,Luis CJ,Marina EJ,Javier CF,Alexis LA,Del Carmen SH,Alfredo RI

    更新日期:2019-01-01 00:00:00

  • Self-assembly of Functional Nanostructures by Short Helical Peptide Building Blocks.

    abstract::The self-assembly of short peptide building blocks into well-ordered nanostructures is a key direction in bionanotechnology. The formation of β -sheet organizations by short peptides is well explored, leading to the development of a wide range of functional assemblies. Likewise, many natural proteinaceous materials, s...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/0929866525666180917163142

    authors: Bera S,Gazit E

    更新日期:2019-01-01 00:00:00

  • The stability curve of hen egg white lysozyme.

    abstract::The physiological stability curve--a plot of the free energy of unfolding versus temperature--is calculated for hen egg white lysozyme from a combination of extrapolated unfolding thermodynamic data from reversible conditions and isothermal titrations with guanidine hydrochloride. The shape of the curve suggests the e...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986606777841163

    authors: Younvanich SS,Britt BM

    更新日期:2006-01-01 00:00:00

  • Therapeutic proteins: A to Z.

    abstract::In recent years, therapeutic proteins have become an important growing class of drugs in the pharmaceutics industry. The development of recombinant DNA technology has caused to appreciation of therapeutic value of many proteins and peptides in medicine. Currently, approximately 100 therapeutic proteins obtained approv...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986652012131112125148

    authors: Ozgur A,Tutar Y

    更新日期:2013-12-01 00:00:00

  • NMAAP1 Maintains M1 Phenotype in Macrophages Through Binding to IP3R and Activating Calcium-related Signaling Pathways.

    abstract:BACKGROUND:NMAAP1 plays a role in regulating macrophage differentiation to the M1 type and exerting antitumoral functions. It is not clear what role and mechanism NMAAP1 does play in the reversal of macrophages from M1 to M2. METHODS:We detected the typing of macrophages with high or low expression of NMAAP1 by QPCR a...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866526666190503105343

    authors: Liu Q,Zhu P,Liu S,Tang M,Wang Y,Tian Y,Jin Z,Li D,Yan D

    更新日期:2019-01-01 00:00:00

  • A structural-energetic basis for B-cell epitope prediction.

    abstract::Structural-energetic analysis of peptide and protein antigens in the context of binding to antibody reveals fundamental differences between the cross-reactions of antipeptide antibody with protein and antiprotein antibody with peptide, providing a physicochemical basis for B-cell epitope prediction as applied to the d...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章,评审

    doi:10.2174/092986606777790502

    authors: Caoili SE

    更新日期:2006-01-01 00:00:00

  • Acyl-gelatins for cell-hybrid biomaterials: preparation of gelatins with high melting point and affinity for hydrophobic surfaces.

    abstract::In the development of cell-hybrid biomaterials, the functional activity of cells depends on the selective binding of cells to artificial ligands on the biomaterials. The extracellular matrix (ECM) is the most important ligand for cell activity. ECM is known to contain collagen, one of whose constituents is gelatin. Al...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866023408436

    authors: Miyamoto K,Chinzei H,Komai T

    更新日期:2002-12-01 00:00:00

  • Investigation on the Interaction between Tetrakis (4-carboxylphenyl) Porphyrin and CopC by Spectroscopy and Docking Methods.

    abstract:BACKGROUND:Recently, the small molecule that inhibits the human copper-trafficking proteins Atox1 and CCS was reported, which suggested that small molecule has an effect on the copper regulation system in the cell. The copper chaperones CopC is regarded as a redox switch and possess barrel structure, thus the interacti...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866526666191204124245

    authors: Song Z,Liu J,Yuan W,Bai Z,Gao N

    更新日期:2020-01-01 00:00:00

  • Characterization of Seed Proteome Profile of Wild and Cultivated Chickpeas of India.

    abstract:BACKGROUND:Chickpea is a widely grown legume in India, Australia, Canada, and Mediterranean regions. Seeds of chickpea are good source of protein for both human and animals. Wild relatives of chickpea (Cicer arietinum) are the potential gene pool for crop improvement; however, very little information is available on th...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/0929866527666200910164118

    authors: Dhar S,Bhattacharjee M,Acharjee S,Baishya D

    更新日期:2020-09-10 00:00:00

  • Design, synthesis and utilization of 1- substituted sulphonyloxy-2-phenyl benzimidazole as a novel peptide coupling reagents.

    abstract::Highly efficient coupling reagents, N-methanesulphonyloxy-2-phenyl benzimidazole and N - p-toluenesulphonyloxy-2-phenyl benzimidazole were designed, synthesized and successfully applied in peptide coupling reactions. Their efficiency was evaluated by synthesizing a number of structurally different amides and peptides ...

    journal_title:Protein and peptide letters

    pub_type: 杂志文章

    doi:10.2174/092986607780090847

    authors: Kokare ND,Nagawade RR,Rane VP,Shinde DB

    更新日期:2007-01-01 00:00:00

  • From the discovery of the reactive site of inhibitors to their sequence to reactivity algorithm.

    abstract::Michael Laskowski Jr. (1930-2004) was a pioneer in the field of Standard mechanism serine proteinase inhibitors. He made numerous important contributions in the field. This article highlights some of his most important contributions such as the discovery of the reactive site in serine proteinase inhibitors, the propos...

    journal_title:Protein and peptide letters

    pub_type: 传,历史文章,杂志文章

    doi:10.2174/0929866054395347

    authors: Qasim MA

    更新日期:2005-07-01 00:00:00