Abstract:
:The studies presented here explore antimicrobial peptide preferential binding behavior for a target pathogen, Escherichia coli O157:H7. A modified immunoassay and surface plasmon resonance were employed to evaluate immobilized peptide binding of whole bacterial cells. The knowledge gained may guide the rational design of peptides with enhanced species binding selectivity.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Soares JW,Kirby R,Morin KM,Mello CMdoi
10.2174/092986608786071049subject
Has Abstractpub_date
2008-01-01 00:00:00pages
1086-93issue
10eissn
0929-8665issn
1875-5305journal_volume
15pub_type
杂志文章abstract::Nicotinamide adenine dinucleotide (NAD) plays an important role in cellular metabolism and acts as hydrideaccepting and hydride-donating coenzymes in energy production. Identification of NAD protein interacting sites can significantly aid in understanding the NAD dependent metabolism and pathways, and it could further...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612800191017
更新日期:2012-05-01 00:00:00
abstract::In recent years, therapeutic proteins have become an important growing class of drugs in the pharmaceutics industry. The development of recombinant DNA technology has caused to appreciation of therapeutic value of many proteins and peptides in medicine. Currently, approximately 100 therapeutic proteins obtained approv...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986652012131112125148
更新日期:2013-12-01 00:00:00
abstract::The cytotoxicity of hPrP[173-195] prion peptide against a neuroblastoma cell model was found independent of its tendency to aggregate over time. Cytosolic and nuclear inclusions of peptide were highlighted by confocal microscopy, suggesting a role as a transcription factor in activating signal transduction pathways in...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609789353655
更新日期:2009-01-01 00:00:00
abstract::To investigate the function of plant plasma membrane, proteins of rice plasma membrane were analyzed and the proteins changed by cold stress were identified. Plasma membrane proteins were purified with an aqueous two-phase partitioning method from root of rice seedlings, and activity of specific H(+)-ATPase localized ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609788490140
更新日期:2009-01-01 00:00:00
abstract::Michael Laskowski Jr. (1930-2004) was a pioneer in the field of Standard mechanism serine proteinase inhibitors. He made numerous important contributions in the field. This article highlights some of his most important contributions such as the discovery of the reactive site in serine proteinase inhibitors, the propos...
journal_title:Protein and peptide letters
pub_type: 传,历史文章,杂志文章
doi:10.2174/0929866054395347
更新日期:2005-07-01 00:00:00
abstract::Protein phosphatase magnesium-dependent 1, delta (PPM1D) is a member of the PPM1 (formerly PP2C) protein phosphatase family, and is induced in response to DNA damage. The overexpression of PPM1D is thought to exert oncogenic effects through the inhibition of tumor suppressor proteins. PPM1D shows high selectivity for ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785849236
更新日期:2008-01-01 00:00:00
abstract::There is a growing body of evidence that the formation and accumulation of advanced glycation end products (AGE) have been known to progress under diabetic conditions, thereby being involved in diabetic vascular complications. Further, we, along with others, have recently found AGE could disturb insulin actions in cul...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610791760423
更新日期:2010-09-01 00:00:00
abstract::We report the physical-chemical characterization of the major alcohol-soluble proteins present in seeds of pearl millet (Pennisetum glaucum) by SDS-PAGE, bidimensional gel electrophoresis, MALDI-TOF/MS and RP-HPLC. We demonstrate the presence of three major prolamins, called A-, B- and C-pennisetin with mass values ar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408724
更新日期:2002-06-01 00:00:00
abstract::The reaction between Arg-Gly-Asp (RGD) and Lys-Gly-Asp (KGD) derivatives with 3,4',5-trihydroxy-trans-stilbene (resveratrol) was investigated. Knowing that resveratrol, RGD as well as KGD analogues inhibit human platelet aggregation in vitro, it was tempting for us to examine whether their coupling products present en...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607782541141
更新日期:2007-01-01 00:00:00
abstract::Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611797642670
更新日期:2011-12-01 00:00:00
abstract::We previously described a potent anthrax toxin inhibitor, based on a phage-library-selected peptide sequence, synthesized as a tetra-branched molecule on a lysine core and further modified for improvement of activity [Pini et al., Biochem. J., 2006, 395, 157]. This branched peptide had very low solubility because of s...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608784966958
更新日期:2008-01-01 00:00:00
abstract::F(0)F(1)-ATPase is a rotary molecular motor. It is well known that the rotary torque is generated by ATP hydrolysis in F(1) but little is known about how it produces the proton-motive force (PMF) in F(0). Here a cross-linking approach was used to estimate the rotary torque produced by PMF. Three mutant E. coli strains...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607779117164
更新日期:2007-01-01 00:00:00
abstract::Bz(NO(2))-Orn(Boc)-OCH(2)CN was synthesized as an amino acid component with effective and successful orthogonal protection for amino acylation of 5'-O-Pivaloyl nucleosides and preparation of substrates for model ribosome reactions. The synthesis was carried out using suitable combinations of the methods of peptide syn...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610791306625
更新日期:2010-07-01 00:00:00
abstract::Norepinephrine-stimulated skin secretions from the Sante Fe frog Leptodactylus laticeps contained high concentrations of a peptide, termed laticeptin, with the primary structure Gly-Val-Val-Asp-Ile-Leu-Lys-Gly-Ala-Ala-Lys-Asp-Leu-Ala-Gly-His-Leu-Ala-Thr-Lys-Val-Met-Asn-Lys-Leu.NH(2). Laticeptin inhibited the growth of...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606775974410
更新日期:2006-01-01 00:00:00
abstract::Here, we show for the first time that non-fibrillar and spherical aggregates produced from PrP-(23-98) in the presence of NADPH plus copper ions are toxic to cultured cells and induce apoptotic signals. It is also confirmed that endogenous cellular PrP isoform is not required for toxicity to occur. ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609787049475
更新日期:2009-01-01 00:00:00
abstract::Adenoregulin is a 33 amino acid antibiotic peptide who belongs to dermaseptin family which is the first vertebrate family to show lethal effects against filamentous fungi, as well as a broad spectrum of pathogenic microorganisms. Synthetic adenoregulin gene was cloned in 2, 4 and 6 tandem repeats and subcloned in pET3...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866053765671
更新日期:2005-05-01 00:00:00
abstract::Coordination of cell growth and cell division is very important for living organisms in order for these to develop harmonically. The present research is concerned with the purification and characterization of a new peptide hormone, namely ZmIGF (Zea mays insulin-like growth factor), which regulates growth and cell div...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611794328636
更新日期:2011-01-01 00:00:00
abstract::Thioredoxin (TRX) catalyzes redox reactions via the reversible oxidation of the conserved active center CGPC and it is involved in multiple biological processes, some of them linked to redox activity while others not. TRX is a globular, thermodynamically stable and monomeric alpha/beta protein with a structure charact...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866522666150707114309
更新日期:2015-01-01 00:00:00
abstract::The description of protein backbone was recently improved with a group of structural fragments called Structural Alphabets instead of the regular three states (Helix, Sheet and Coil) secondary structure description. Protein Blocks is one of the Structural Alphabets used to describe each and every region of protein bac...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866511320030003
更新日期:2013-03-01 00:00:00
abstract::In the development of cell-hybrid biomaterials, the functional activity of cells depends on the selective binding of cells to artificial ligands on the biomaterials. The extracellular matrix (ECM) is the most important ligand for cell activity. ECM is known to contain collagen, one of whose constituents is gelatin. Al...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408436
更新日期:2002-12-01 00:00:00
abstract::Migraine is a common neurologic condition marked by recurrent episodes of headache. Its pathophysiology is highly complex involving neuronal, inflammatory and vascular mechanisms. The Renin-Angiotensin System (RAS) can modulate all these mechanism, being a potential pharmacological target for migraine treatment. We ca...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200129160136
更新日期:2020-01-01 00:00:00
abstract::We investigated the in vitro antibiotic activity of the 19-amino acid antimicrobial peptide HP (2-20), derived from the N-terminus of Helicobacter pylori Ribosomal Protein L1 (RPL1), against antibiotic susceptible and resistant pathogens from a patient with gallstones. HP (2-20) was active against antibiotic-susceptib...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606774502018
更新日期:2006-01-01 00:00:00
abstract::The formation of amyloid-like fibrils was studied by using the well-known serine protease trypsin as a model protein in the presence of ethanol as organic solvent. Trypsin forms amyloid-like fibrils in aqueous ethanol at pH = 7.0. The dye Congo red (CR) was used to detect the presence of amyloid-like fibrils in the sa...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866522666151002154324
更新日期:2015-01-01 00:00:00
abstract::The study of rat proteins is an indispensable task in experimental medicine and drug development. The function of a rat protein is closely related to its subcellular location. Based on the above concept, we construct the benchmark rat proteins dataset and develop a combined approach for predicting the subcellular loca...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611795222768
更新日期:2011-06-01 00:00:00
abstract::Ewing's Sarcoma transcript-2 (EAT2) also known as SH2D1B is involved in regulation of signalling lymphocytic activation molecule (SLAM) family receptor functions. Cytoplasmic tails of SLAM family receptors contain tyrosine residues which mediate the downstream signal transduction through their phosphorylation. EAT2, c...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666160831162239
更新日期:2016-01-01 00:00:00
abstract::Sexual maturation and olfactory bulb defects found in prokineticin 2 (Pk2) and prokineticin receptor 2 (Pkr2) mutant mice resembling the phenotypic characteristics of Kallmann syndrome (KS), gave rise to the question of whether these genes would have a role in KS pathogenesis. Later, mutations in both genes were ident...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666160517123331
更新日期:2016-01-01 00:00:00
abstract::Since their recent identification, eukaryotic Hsp70 nucleotide exchange factors (NEFs) have gained increasing interest due to their engagement in vital cellular processes. Here, I summarize our current knowledge of their mechanisms of action, regulations and cellular functions as well as their relevance for human dise...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986609788490186
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Multiple Sequence Alignment (MSA) is a fundamental task in bioinformatics and is required for many biological analysis tasks. The more accurate the alignments are, the more credible the downstream analyses. Most protein MSA algorithms realign an alignment to refine it by dividing it into two groups horizonta...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190806143959
更新日期:2020-01-01 00:00:00
abstract::Tumor vasculatures express high levels of α(V)β(3)/α(V)β(5) and α(5)β(1) integrins. Peptide containing the RGD (Arg-Gly-Asp) sequence, which is present in ligands of integrins, is effective in targeting therapeutic reagents to tumor vascular endothelium. In this study, we investigated whether the biological activity o...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2011-06-15 00:00:00
abstract::A novel cellulase was isolated from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius ATCC27009 grown in medium containing carboxymethylcellulose. The enzyme is a glycosylated monomer of 56.2 kDa, relatively thermostable, with optimal pH and temperature of 4.0 and 65 degrees C, respectively. Enzymatic as...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785849209
更新日期:2008-01-01 00:00:00