Abstract:
:Nicotinamide adenine dinucleotide (NAD) plays an important role in cellular metabolism and acts as hydrideaccepting and hydride-donating coenzymes in energy production. Identification of NAD protein interacting sites can significantly aid in understanding the NAD dependent metabolism and pathways, and it could further contribute useful information for drug development. In this study, a computational method is proposed to predict NAD-protein interacting sites using the sequence information and structure-based information. All models developed in this work are evaluated using the 7-fold cross validation technique. Results show that using the position specific scoring matrix (PSSM) as an input feature is quite encouraging for predicting NAD interacting sites. After considering the unbalance dataset, the ensemble support vector machine (SVM), which is an assembly of many individual SVM classifiers, is developed to predict the NAD interacting sites. It was observed that the overall accuracy (Acc) thus obtained was 87.31% with Matthew's correlation coefficient (MCC) equal to 0.56. In contrast, the corresponding rate by the single SVM approach was only 80.86% with MCC of 0.38. These results indicated that the prediction accuracy could be remarkably improved via the ensemble SVM classifier approach.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Wang X,Wang CC,Zhang YQ,Mi G,Zhang J,Li MLdoi
10.2174/092986612800191017subject
Has Abstractpub_date
2012-05-01 00:00:00pages
559-66issue
5eissn
0929-8665issn
1875-5305pii
PPL-EPUB-20120207-007journal_volume
19pub_type
杂志文章abstract:BACKGROUND:Fruit juice clarification is a challenging aspect of beverage industry which needs to be addressed for economical and hygienic production of fruit juices. OBJECTIVE:Current study is focused on the complete purification, characterization and thermodynamic analysis of an efficient mannanase enzyme to analyze ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200916142305
更新日期:2020-09-16 00:00:00
abstract::The renin-angiotensin system (RAS) has two different axes, the classical one with the effector peptide angiotensin II and the new one with the effector peptide angiotensin (1-7). Both peptides have been shown to be involved in the pathogenesis of diabetes mellitus and its consequences, nephropathy, retinopathy and car...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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更新日期:2017-11-17 00:00:00
abstract::Supramolecular self-assembled functional materials comprised of cyclic dipeptide building blocks have excellent prospects for biotechnology applications due to their exceptional structural rigidity, morphological flexibility, ease of preparation and modification. Although the pharmacological uses of many natural cycli...
journal_title:Protein and peptide letters
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更新日期:2020-01-01 00:00:00
abstract::Bz(NO(2))-Orn(Boc)-OCH(2)CN was synthesized as an amino acid component with effective and successful orthogonal protection for amino acylation of 5'-O-Pivaloyl nucleosides and preparation of substrates for model ribosome reactions. The synthesis was carried out using suitable combinations of the methods of peptide syn...
journal_title:Protein and peptide letters
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更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Type VI Secretion System (T6SS) has been found in approximately onequarter of the gram-negative bacterial species, and its structural characteristics appear to slightly differ from species to species. The genes encoding T6SS are designated as type six secretion A-M (tssA-M). The expression of the tss gene cl...
journal_title:Protein and peptide letters
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doi:10.2174/0929866526666190119121859
更新日期:2019-01-01 00:00:00
abstract::Polymyositis is an inflammatory myopathy characterized by muscle invasion of T-cells penetrating the basal lamina and displacing the plasma membrane of normal muscle fibers. In order to understand the different adhesive mechanisms at the T-cell surface, Schubert randomly selected 19 proteins expressed at the T-cell su...
journal_title:Protein and peptide letters
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doi:10.2174/092986612801619534
更新日期:2012-08-01 00:00:00
abstract::We report a new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed a truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and eval...
journal_title:Protein and peptide letters
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更新日期:2006-01-01 00:00:00
abstract::Sry-related box (Sox) transcription factors share a conserved high-mobility-group box domain (HMG-domain) that binds DNA in the minor groove and bends DNA for further assembly of transcriptional machineries. During organogenesis, each member of the Sox family triggers a specific cell lineage differentiation, indicatin...
journal_title:Protein and peptide letters
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更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:Microalgae have been suggested as promising feedstocks of significant biotechnological interest due to their enormous potential for the sustainable production of industrially valuable compounds such as lipids/fatty acids, proteins, metabolites, pigments, and biofuels. However, exploitation of algal biomass f...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00
abstract::Coordination of cell growth and cell division is very important for living organisms in order for these to develop harmonically. The present research is concerned with the purification and characterization of a new peptide hormone, namely ZmIGF (Zea mays insulin-like growth factor), which regulates growth and cell div...
journal_title:Protein and peptide letters
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更新日期:2011-01-01 00:00:00
abstract::Marine species are comprising about a half of the whole global biodiversity; the sea offers an enormous resource for novel bioactive compounds. Several of the marine origin species show multifunctional bioactivities and characteristics that are useful for a discovery and/or reinvention of biologically active compounds...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866523666160802160222
更新日期:2017-01-01 00:00:00
abstract::Alpha-gliadins isolated by carboxymethylcellulose chromatography contain noncovalently bound glucose probably due to contaminating proteoglycans and to material shed from the column. Traces of carbohydrate remain strongly bound to alpha-gliadins even after harsh denaturation, but our results indicate alpha-gliadins ar...
journal_title:Protein and peptide letters
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doi:10.2174/0929866023408995
更新日期:2002-02-01 00:00:00
abstract::Loxosceles intermedia spider venom was subjected to proteomic analysis through a MudPIT shot-gun approach to identify the protein composition. Were identified 39 proteins which seem to responsible by the lesion of different types of tissues, to some physiopathological actions and by the prevention of structural damage...
journal_title:Protein and peptide letters
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更新日期:2009-01-01 00:00:00
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journal_title:Protein and peptide letters
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doi:10.2174/09298665113206660110
更新日期:2014-04-01 00:00:00
abstract::Phage Virion Proteins (PVP) are essential materials of bacteriophage, which participate in a series of biological processes. Accurate identification of phage virion proteins is helpful to understand the mechanism of interaction between the phage and its host bacteria. Since experimental method is labor intensive and t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190410124642
更新日期:2020-01-01 00:00:00
abstract::Quantum mechanical fragment molecular orbital calculations have been performed for receptor binding of the hemagglutinin protein of the recently pandemic influenza 2009 H1N1, A/swine/Iowa/1930, and A/Puerto Rico/8/1934 viruses to α2-6 linked sialyloligosaccharides, as analogs of human receptors. The strongest receptor...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611794927893
更新日期:2011-05-01 00:00:00
abstract::Human histatins are histidine-rich, low molecular weight salivary proteins that contribute to the immune system of the oral cavity. In this work, nucleotide sequences of the HIS1 (coding for histatin 1) and HIS2 (coding for histatin 3) genes, homologous to the human ones, have been sequenced and analysed in five prima...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610791306715
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Nε-acetyl L-α lysine is an unusual acetylated di-amino acid synthesized and accumulated by certain halophiles under osmotic stress. Osmolytes are generally known to protect proteins and other cellular components under various stress conditions. OBJECTIVE:The structural and functional stability imparted by N...
journal_title:Protein and peptide letters
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doi:10.2174/0929866526666191105130041
更新日期:2020-01-01 00:00:00
abstract::There is accumulating evidence that proteinuria is not merely a biomarker for the progression of chronic kidney disease (CKD), but also a mediator of this devastating disorder. Indeed, albumin, one of the major components found in proteinuria, causes proinflammatory and profibrotic changes in cultured proximal tubular...
journal_title:Protein and peptide letters
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更新日期:2010-01-01 00:00:00
abstract::DrTI was effective against trypsin-like enzymes from A. kuehniella and C. cephalonica, however an artificial diet was insufficient to affect the survival and body weight of either insect. The inhibitor stimulated chymotrypsin-like enzymes and probably induced the synthesis of enzymes insensitive to TLCK in neonate lar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
abstract::Red blood cell (RBC) encapsulated L-asparaginase is a novel therapeutic for the treatment of asparagine auxotrophic malignancies. The enzyme-loaded red blood cells function as bioreactors to deplete bloodstream substrate. This delivery system provides improved pharmacodynamics with protection from circulating proteoly...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:
更新日期:2013-04-01 00:00:00
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journal_title:Protein and peptide letters
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journal_title:Protein and peptide letters
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abstract::A recombinant human parathyroid hormone (rhPTH) fragment (Gly1-Gln26-rhPTH(1-34)) which contains two amino acids substitutions (Gly1 and Gln26) was acquired through Escherichia coli expression system using a soluble fusion protein strategy. The soluble fusion protein MBP-Gly1-Gln26-rhPTH(1-34) was harvested after puri...
journal_title:Protein and peptide letters
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更新日期:2008-01-01 00:00:00
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journal_title:Protein and peptide letters
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
abstract::We used a de novo designed, beta-hairpin forming T1 peptide as a model to investigate the kinetics of peptide fibrogenesis by a combination of light scattering (LS), circular dichroism (CD), fluorescence, and atomic force microscopy (AFM). The results demonstrate that the T1 fibrogenesis undergoes a consecutive stepwi...
journal_title:Protein and peptide letters
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更新日期:2010-04-01 00:00:00
abstract::Although the tertiary structures of mitochondrial cytochromes c (cyts c) seem to be remarkably similar, there are variations in their amino acid sequences, stability and functional properties. GdnHCl-induced unfolding experiments on engineered yeast and horse cyt c were carried out with the aim to to clarify, at molec...
journal_title:Protein and peptide letters
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更新日期:2007-01-01 00:00:00
abstract::Prediction of protein structure from its amino acid sequence is still a challenging problem. The complete physicochemical understanding of protein folding is essential for the accurate structure prediction. Knowledge of residue solvent accessibility gives useful insights into protein structure prediction and function ...
journal_title:Protein and peptide letters
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更新日期:2012-01-01 00:00:00
abstract::Since their recent identification, eukaryotic Hsp70 nucleotide exchange factors (NEFs) have gained increasing interest due to their engagement in vital cellular processes. Here, I summarize our current knowledge of their mechanisms of action, regulations and cellular functions as well as their relevance for human dise...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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abstract::The self-assembly of short peptide building blocks into well-ordered nanostructures is a key direction in bionanotechnology. The formation of β -sheet organizations by short peptides is well explored, leading to the development of a wide range of functional assemblies. Likewise, many natural proteinaceous materials, s...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
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更新日期:2019-01-01 00:00:00