Probing the effect of mutations on cytochrome C stability.

Abstract:

:Although the tertiary structures of mitochondrial cytochromes c (cyts c) seem to be remarkably similar, there are variations in their amino acid sequences, stability and functional properties. GdnHCl-induced unfolding experiments on engineered yeast and horse cyt c were carried out with the aim to to clarify, at molecular level, some aspects concerning the stability of this class of proteins. The results obtained are discussed in the light of the three-dimensional structures of the two proteins.

journal_name

Protein Pept Lett

authors

Agueci F,Polticelli F,Sinibaldi F,Piro MC,Santucci R,Fiorucci L

doi

10.2174/092986607780363989

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

335-9

issue

4

eissn

0929-8665

issn

1875-5305

journal_volume

14

pub_type

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