Abstract:
:DrTI was effective against trypsin-like enzymes from A. kuehniella and C. cephalonica, however an artificial diet was insufficient to affect the survival and body weight of either insect. The inhibitor stimulated chymotrypsin-like enzymes and probably induced the synthesis of enzymes insensitive to TLCK in neonate larvae.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Macedo ML,Pando SC,Chevreuil LR,Marangoni Sdoi
10.2174/092986609789839395subject
Has Abstractpub_date
2009-01-01 00:00:00pages
1459-65issue
12eissn
0929-8665issn
1875-5305pii
0042journal_volume
16pub_type
杂志文章abstract::A proof-of-principle methodology is presented in which all commercially-available cysteine (Cys) and selenocysteine (Sec) solid phase peptide synthesis (SPPS) derivatives are synthesized in high yield from easily prepared protected dichalcogenide precursors. A Zn-mediated biphasic reduction process applied to a series...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::The search for potential inhibitors that target so far unexplored bacterial enzyme mono-N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) has stimulated a development of methodology for quick and efficient preparation of mono-N-acylated 2,6-diaminopimelic acid (DAP) derivatives bearing the different carboxyl gro...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790780387
更新日期:2010-03-01 00:00:00
abstract::Transgelin was solubly expressed in E. coli. Crystals of transgelin have been grown at 291K using sodium formate or PEG-4000 as precipitants. X-ray diffraction by the crystal extends to 2.3 A resolution. The crystal belongs to the space group P2(1), with the unit cell parameters a=39.3, b=61.9, c=56.0 A and beta=90.2 ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607779816122
更新日期:2007-01-01 00:00:00
abstract::Arginine kinase is an essential enzyme which is closely related to energy metabolism in marine invertebrates. Arginine kinase provides a significant role in quick response to environmental change and stress. In this study, we simulated a tertiary structure of Sepia pharaonis arginine kinase (SPAK) based on the gene se...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666160331144049
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Paraoxonase (PON; arilesterase, [EC 3.1.8.1]) is an enzyme from the group arilesterases (ARE). This enzyme is capable of hydrolyzing paraoxone which is the active metabolite of parathion, an organic phosphorus insecticide. PON activity was found to be low in individuals prone to development of atherosclerosi...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190124144622
更新日期:2019-01-01 00:00:00
abstract::The role of interaction between Asn259 (catalytic domain) with Gln821 (C-terminal domain) in PeptidaseN was investigated. The k(cat) of PeptidaseN containing Asn259Asp or Gln821Glu is enhanced whereas it is suppressed in Asn259AspGln821Glu. Structural analysis shows this interaction to change the relative disposition ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609787848081
更新日期:2009-01-01 00:00:00
abstract::BmK ITa1 cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni(2+)-IDA-sepharose 6B column was toxic for insect, which indicated t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408472
更新日期:2002-10-01 00:00:00
abstract::Since their recent identification, eukaryotic Hsp70 nucleotide exchange factors (NEFs) have gained increasing interest due to their engagement in vital cellular processes. Here, I summarize our current knowledge of their mechanisms of action, regulations and cellular functions as well as their relevance for human dise...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986609788490186
更新日期:2009-01-01 00:00:00
abstract::The renin-angiotensin system (RAS) has two different axes, the classical one with the effector peptide angiotensin II and the new one with the effector peptide angiotensin (1-7). Both peptides have been shown to be involved in the pathogenesis of diabetes mellitus and its consequences, nephropathy, retinopathy and car...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866524666170728144357
更新日期:2017-11-17 00:00:00
abstract:BACKGROUND:The O. tesota lectin PF2 is a tetrameric protein with subunits of 33 kDa that recognizes only complex carbohydrates, resistant to proteolytic enzymes and has insecticidal activity against Phaseolus beans pest. OBJECTIVE:To explore PF2 lectin features at different protein structural levels and to evaluate th...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200813204303
更新日期:2020-08-13 00:00:00
abstract::The cytotoxicity of hPrP[173-195] prion peptide against a neuroblastoma cell model was found independent of its tendency to aggregate over time. Cytosolic and nuclear inclusions of peptide were highlighted by confocal microscopy, suggesting a role as a transcription factor in activating signal transduction pathways in...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609789353655
更新日期:2009-01-01 00:00:00
abstract::The study of rat proteins is an indispensable task in experimental medicine and drug development. The function of a rat protein is closely related to its subcellular location. Based on the above concept, we construct the benchmark rat proteins dataset and develop a combined approach for predicting the subcellular loca...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611795222768
更新日期:2011-06-01 00:00:00
abstract::Familial mediterranean fever (FMF) is a systemic disorder characterized by recurrent attacks of fever and polyserositis. In FMF, several pro-inflammatory cytokines have been found to be elevated during the attacks. In recent years, it is shown that some proteins originated from adipose tissue play important role in in...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610792231555
更新日期:2010-10-01 00:00:00
abstract::We report a new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed a truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and eval...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606775101553
更新日期:2006-01-01 00:00:00
abstract::AB204 is an Activin/BMP2 chimera, which has been found to exhibit a higher activity than Bone Morphogenetic Protein 2 (BMP2) in osteogenic activity. To prepare AB204 for its preclinical studies, AB204 has been characterized in various formulation buffers. We observed that AB204 purified by ion-exchange chromatography ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986652105140218110302
更新日期:2014-05-01 00:00:00
abstract::Classification of newly determined protein structures is important in understanding their function and mechanism of action. Currently available methods employ a global structure alignment strategy and are computationally expensive. We propose a two-step methodology with a quick screen to significantly reduce the numbe...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607781483877
更新日期:2007-01-01 00:00:00
abstract::Marine species are comprising about a half of the whole global biodiversity; the sea offers an enormous resource for novel bioactive compounds. Several of the marine origin species show multifunctional bioactivities and characteristics that are useful for a discovery and/or reinvention of biologically active compounds...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866523666160802160222
更新日期:2017-01-01 00:00:00
abstract::Free radicals induce protein modifications, often associated to many biological phenomena. This mini-review overviews the approaches we have used to elucidate the radical-induced damages on sulfur-containing enzymes, such as ribonuclease and lysozyme, and the possible post-translational mechanism of the damage to anot...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986607781483840
更新日期:2007-01-01 00:00:00
abstract::Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866054696163
更新日期:2005-10-01 00:00:00
abstract::Antimicrobial peptides (AMPs) represent a large and ubiquitous group of peptides. The current crisis in antibiotic therapy has led to an intensified search for new antimicrobial agents. In this regard, scorpion venom constitutes a rich source of biologically active peptides including AMPs. In the present study, the pu...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986652202150128143048
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Development of proteins and peptides into drugs has been considered as a promising strategy to target certain diseases. However, only few proteins and peptides have been approved as new drugs into the market each year. One major problem is that proteins and peptides often exhibit short plasma half-life times...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866525666180530122835
更新日期:2018-01-01 00:00:00
abstract::After consideration of the tertiary structure of the pY+3 binding pocket in SH2 domains, isoleucine of Ac-pYEEIE was replaced with various unnatural aliphatic amino acids and the binding affinities for Lck, Src, and Fyn SH2 domains were measured. We determined the characteristics of the amino acid at the pY+3 position...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866043406337
更新日期:2004-12-01 00:00:00
abstract::Protein palmitoylation is an important and common post-translational lipid modification of proteins and plays a critical role in various cellular processes. Identification of Palmitoylation sites is fundamental to decipher the mechanisms of these biological processes. However, experimental determination of palmitoylat...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611794475084
更新日期:2011-02-01 00:00:00
abstract::Apomyoglobin (apoMb) folds through at least two partially folded forms that are detected both as transient intermediates during folding/unfolding kinetics or as stable intermediates at equilibrium. Here, I summarize the results of recent kinetic studies, which combined with detailed characterizations of equilibrium fo...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866053587174
更新日期:2005-04-01 00:00:00
abstract::3-Isopropylmalate dehydrogenase (IPMDH) from Mycobacterium tuberculosis (Mtb) may be a target for specific drugs against this pathogenic bacterium. We have expressed and purified Mtb IPMDH and determined its physicalchemical and enzymological properties. Size-exclusion chromatography and dynamic light scattering measu...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::The physiological stability curve--a plot of the free energy of unfolding versus temperature--is calculated for hen egg white lysozyme from a combination of extrapolated unfolding thermodynamic data from reversible conditions and isothermal titrations with guanidine hydrochloride. The shape of the curve suggests the e...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777841163
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Bursa of Fabricius plays the vital functions on B cell development and antibody production in poultry. The bursal-derived peptide plays the essential roles on avian immature B cell development. OBJECTIVES:Here we explored the functions of the recently reported bursal nonapeptide (BP9) on the antibody produc...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666190730101455
更新日期:2019-01-01 00:00:00
abstract::S-adenosyl-l-homocysteine hydrolase from a malaria parasite Plasmodium falciparum (PfSAHH) has been crystallized by the vapor diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 76.66 A, b = 86.31 A, and c = 335.6 A. There are four subunits (one tetramer) per as...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866043478248
更新日期:2004-04-01 00:00:00
abstract:BACKGROUND:Protealysin, a zinc metalloprotease of Serratia proteamaculans, is the prototype of a new group within the peptidase family M4. Protealysin-like proteases (PLPs) are widely spread in bacteria but are also found in fungi and archaea. The biological functions of PLPs have not been well studied, but published d...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666181212114907
更新日期:2019-01-01 00:00:00
abstract::We report the stepwise transformation of a linear peptide epitope recognized by the anti-transforming growth factor alpha monoclonal antibody Tab2 into peptomers and finally into peptoid analogs. The key experiment in this study is the substitution analysis in which each position of the peptide is exchanged by a set o...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777841299
更新日期:2006-01-01 00:00:00