Abstract:
:Bz(NO(2))-Orn(Boc)-OCH(2)CN was synthesized as an amino acid component with effective and successful orthogonal protection for amino acylation of 5'-O-Pivaloyl nucleosides and preparation of substrates for model ribosome reactions. The synthesis was carried out using suitable combinations of the methods of peptide synthesis and modification of amino acids.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Bayryamov SG,Vassilev NG,Petkov DDdoi
10.2174/092986610791306625subject
Has Abstractpub_date
2010-07-01 00:00:00pages
889-98issue
7eissn
0929-8665issn
1875-5305pii
BSP/ PPL/ E pub/0133journal_volume
17pub_type
杂志文章abstract:BACKGROUND:Malignant tumor is still one of the important diseases worldwide, cytotoxic CD8+ T lymphocytes (CTLs) play an important role in killing tumor cells. OBJECTIVE:To enhance the immune response of our previously identified HLA-A2-restricted CTL epitopes, we designed a multiepitope YL66. METHOD:The fusion prote...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866524666170419152700
更新日期:2017-01-01 00:00:00
abstract::BmK ITa1 cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni(2+)-IDA-sepharose 6B column was toxic for insect, which indicated t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408472
更新日期:2002-10-01 00:00:00
abstract::In general, halophilic proteins are stable only in the presence of salts at high concentrations. Not only is high salt concentration important for structural stability of halophilic proteins, but also refolding of a denatured halophilic protein requires high salt concentration. This review summarizes the importance of...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866043478220
更新日期:2004-04-01 00:00:00
abstract::Discriminating cell wall lytic enzymes from non lytic enzymes is a very important task for curing bacterial infections. In this paper, based on Chou's amphiphilic pseudo amino acid composition, we develop fisher-discriminant based classifier to predict cell wall lytic enzymes. Experiments show that 66.7% sensitivity w...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609787848045
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Hepatitis C Virus (HCV) is a major cause of chronic liver disease in the world. Many studies showed that T-cell responses to HCV Nonstructural Protein 3 (NS3) play an important role in clearing acute HCV infections, thus NS3 can be considered as a suitable candidate antigen for development of a HCV therapeut...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866525666180925145304
更新日期:2018-01-01 00:00:00
abstract::A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were c...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866043478482
更新日期:2004-02-01 00:00:00
abstract::Kallikrein-related peptidases (KLKs) are trypsin-like and chymotrypsin-like serine proteases which are expressed in several tissues. Their activity is tightly controlled by inhibitors including members of the serine protease Kazal-type (SPINK) family. These enzymes are promising targets for the treatment of skin desqu...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866511320100003
更新日期:2013-10-01 00:00:00
abstract::Alpha-gliadins isolated by carboxymethylcellulose chromatography contain noncovalently bound glucose probably due to contaminating proteoglycans and to material shed from the column. Traces of carbohydrate remain strongly bound to alpha-gliadins even after harsh denaturation, but our results indicate alpha-gliadins ar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408995
更新日期:2002-02-01 00:00:00
abstract::The polypeptide leptin exerts a multitude of regulatory functions. It has been implicated in the pathophysiology of inflammatory, metabolic and psychiatric disorders and has been found to be differentially expressed in men and women. Although a clear increase of leptin levels with age has been repeatedly observed in m...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866511009011436
更新日期:2010-11-01 00:00:00
abstract::Neuronal tau, through its proline-rich domain and the microtubule binding domain, binds to RNA non-sequence-specifically via electrostatic interaction. This binding inhibits the activity of tau. Tau and RNA were also found to co-localize in SH-SY5Y cells suggesting that RNA has opportunities to interact with tau in ce...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777790566
更新日期:2006-01-01 00:00:00
abstract::Nicotinamide adenine dinucleotide (NAD) plays an important role in cellular metabolism and acts as hydrideaccepting and hydride-donating coenzymes in energy production. Identification of NAD protein interacting sites can significantly aid in understanding the NAD dependent metabolism and pathways, and it could further...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612800191017
更新日期:2012-05-01 00:00:00
abstract::Sexual maturation and olfactory bulb defects found in prokineticin 2 (Pk2) and prokineticin receptor 2 (Pkr2) mutant mice resembling the phenotypic characteristics of Kallmann syndrome (KS), gave rise to the question of whether these genes would have a role in KS pathogenesis. Later, mutations in both genes were ident...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666160517123331
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:The Renin-Angiotensin System (RAS) has emerged as being related to vascular disease. Recently the RAS has been associated with obesity, diabetes, and even cancer. OBJECTIVE:This review and Bioinformatics analyses focuses on the investigation of Angiotensinconverting enzymes (ACE and ACE2) as therapeutical...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866524666170815161621
更新日期:2017-11-17 00:00:00
abstract::Porins are integral membrane proteins found in the outer membrane of bacteria, mitochondria and chloroplasts. Herein, we have reviewed sequence and structural understanding about bacterial porins. The first porin structure from Rhodobacter capsulatus at 1.8 Å resolution in 1991 till the recent structural advancement, ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866521666140827110755
更新日期:2015-01-01 00:00:00
abstract::Although macromolecular crystallography is a widely supported technique at synchrotron radiation facilities throughout the world, there are, in comparison, only very few beamlines dedicated to small-molecule crystallography. This limited provision is despite the increasing demand for beamtime from the chemical crystal...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866523666160115131217
更新日期:2016-01-01 00:00:00
abstract::Self-Interacting Proteins (SIPs), whose two or more copies can interact with each other, have significant roles in cellular functions and evolution of Protein Interaction Networks (PINs). Knowing whether a protein can act on itself is important to understand its functions. Previous studies on SIPs have focused on thei...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866527666191227141713
更新日期:2020-01-01 00:00:00
abstract::Transgelin was solubly expressed in E. coli. Crystals of transgelin have been grown at 291K using sodium formate or PEG-4000 as precipitants. X-ray diffraction by the crystal extends to 2.3 A resolution. The crystal belongs to the space group P2(1), with the unit cell parameters a=39.3, b=61.9, c=56.0 A and beta=90.2 ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607779816122
更新日期:2007-01-01 00:00:00
abstract::C-terminal peptide alpha-thioesters are valuable intermediates in the synthesis/semisynthesis of proteins by native chemical ligation. They are prepared either by solid-phase peptide synthesis (SPPS) or biosynthetically by protein splicing techniques. The present paper reviews the different methods available for the c...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866054864166
更新日期:2005-11-01 00:00:00
abstract::The description of protein backbone was recently improved with a group of structural fragments called Structural Alphabets instead of the regular three states (Helix, Sheet and Coil) secondary structure description. Protein Blocks is one of the Structural Alphabets used to describe each and every region of protein bac...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866511320030003
更新日期:2013-03-01 00:00:00
abstract::To investigate the function of plant plasma membrane, proteins of rice plasma membrane were analyzed and the proteins changed by cold stress were identified. Plasma membrane proteins were purified with an aqueous two-phase partitioning method from root of rice seedlings, and activity of specific H(+)-ATPase localized ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609788490140
更新日期:2009-01-01 00:00:00
abstract::We report the stepwise transformation of a linear peptide epitope recognized by the anti-transforming growth factor alpha monoclonal antibody Tab2 into peptomers and finally into peptoid analogs. The key experiment in this study is the substitution analysis in which each position of the peptide is exchanged by a set o...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777841299
更新日期:2006-01-01 00:00:00
abstract::It is important to understand the conformational biases that are present in unfolded states to understand protein folding. In this context, it is surprising that even a short tripeptide like AFA samples folded/ordered conformation as demonstrated recently by NMR experiments of the peptide in aqueous solution at 280 K....
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986607780989886
更新日期:2007-01-01 00:00:00
abstract::In the last few decades, novel drug delivery strategies have been a big priority to the formulation scientists. Peptides and proteins have drawn a special attention for their wide scope in the area. Serum albumin, transferrin, recom- binant proteins, virus capsids etc. are used as carrier for drug and biomolecules. Co...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866521666140804160907
更新日期:2014-01-01 00:00:00
abstract::VML is a lectin from Vatairea macrocarpa seeds that has various biological activities. Here, we describe three new lectin isoforms from V. macrocarpa identified through genomic DNA analysis. One of these isoforms has high similarity to VML, while another that has noteworthy differences. We have denoted the new isoform...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113209990049
更新日期:2013-11-01 00:00:00
abstract::A computer-aided docking study was carried out to quickly clarify the binding structure of the ligand-receptor complex between bisphenol A (BPA), a well-known endocrine disruptor, and estrogen-related receptor gamma (ERRgamma). The resulting complex indicated that BPA binds to the ligand-binding pocket of ERRgamma wit...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608783744261
更新日期:2008-01-01 00:00:00
abstract::Antimicrobial peptides (AMPs) represent a large and ubiquitous group of peptides. The current crisis in antibiotic therapy has led to an intensified search for new antimicrobial agents. In this regard, scorpion venom constitutes a rich source of biologically active peptides including AMPs. In the present study, the pu...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986652202150128143048
更新日期:2015-01-01 00:00:00
abstract::Calreticulin is a soluble endoplasmic reticulum chaperone, which has a relatively low melting point due to its remarkable structure with a relatively high content of flexible structural elements. Using far ultraviolet circular dichroism (CD) spectroscopy and a fluorescent dye binding thermal shift assay, we have inves...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866511320050009
更新日期:2013-05-01 00:00:00
abstract::Solid state nuclear magnetic resonance (NMR) has developed into one of the most informative and direct experimental approaches to the characterization of the molecular structures of amyloid fibrils, including those associated with Alzheimer's disease. In this article, essential aspects of solid state NMR methods are d...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986606775338470
更新日期:2006-01-01 00:00:00
abstract::Nudix hydrolases catalyze the hydrolysis of nucleoside diphosphates linked to other moieties X, and contain the sequence motif or Nudix box, GX5EX7REUXEEXGU. The mechanisms of Nudix hydrolases are highly diverse in the position on the substrate. In this paper, we examined the sequences and structures of the MutT/Nudix...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608783330350
更新日期:2008-01-01 00:00:00
abstract::The identification of interfaces in protein complexes is effective for the elucidation of protein function and helps us to understand their roles in biological processes. With the exponentially growing amount of protein sequence data, an exploration of new methods that predict protein interaction sites based solely on...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986613804725208
更新日期:2013-02-01 00:00:00