Abstract:
:Adenoregulin is a 33 amino acid antibiotic peptide who belongs to dermaseptin family which is the first vertebrate family to show lethal effects against filamentous fungi, as well as a broad spectrum of pathogenic microorganisms. Synthetic adenoregulin gene was cloned in 2, 4 and 6 tandem repeats and subcloned in pET32a and pET22b vectors. Recombinant plasmids were transformed into E. coli BL21(DE3), Fusion proteins of Trx-ADR1, Trx-ADR2 and Trx-ADR4 could be expressed after the hosts were induced by IPTG, but the expression level decreased dramatically with the number of tandem repeats increased. ADR1, ADR4 and ADR6 could not be expressed by E. coli without carrier proteins. But for Pichia pastoris GS115, ADR1 and ADR6 in the fermentation broth of the hosts could be detected by ELISA, and the bactericidal activities could also be observed.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Zhou Y,Cao W,Wang J,Ma Y,Wei Ddoi
10.2174/0929866053765671subject
Has Abstractpub_date
2005-05-01 00:00:00pages
349-55issue
4eissn
0929-8665issn
1875-5305journal_volume
12pub_type
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