Abstract:
:Heparin is naturally occurring polysaccharides which interacts with seminal plasma proteins and regulate multiple steps in fertilization process. Qualitative and quantitative information regarding the affinity for heparin-seminal plasma proteins interactions is not generally well documented and there are no reports of a comprehensive analysis of these interactions in human seminal plasma. Such information should improve our understanding of how GAGs especially heparin present in the reproductive tract regulate fertilization. In this study, we use SPR to study interactions of heparin with various seminal plasma heparin-binding proteins (HBPs). HBPs like lactoferrin (LF), fibronectin fragment (FNIII), semenogelinI (SGI) and prostate specific antigen (PSA) all bind heparin with different binding kinetics and affinities. Kinetic data suggests that FNIII binds heparin with a high affinity (KD=3.2 nM), while PSA binds heparin with a micromolar affinity (KD=11.1 μM). Preincubation of SGI with heparin inhibits the binding of SGI to immobilized PSA in a dose-dependent manner, while FNIII incubated with heparin binds with an increased affinity to PSA. Solution-competition studies show that the minimum size of a heparin oligosaccharide capable of binding with PSA is greater than a tetrasaccharide, with LF and SGI is larger than a hexasaccharide and for FNIII is larger than an octasaccharide.
journal_name
Protein Pept Lettjournal_title
Protein and peptide lettersauthors
Kumar V,Yadav VK,Hassan MI,Singh AK,Dey S,Singh S,Singh TP,Yadav Sdoi
10.2174/092986612801619525subject
Has Abstractpub_date
2012-08-01 00:00:00pages
795-803issue
8eissn
0929-8665issn
1875-5305pii
PPL-EPUB-20120704-07journal_volume
19pub_type
杂志文章abstract::The stability of Merrifield linker in Fmoc deprotection process was quantitatively investigated by establishing working curve of two major decomposition components from two resin bound dipeptide models. By sampling reaction solution and analyzing with RP-HPLC, decomposition rate was determined. The results indicated t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986613804725343
更新日期:2013-02-01 00:00:00
abstract::The immunoglobulin fold (Ig-fold) is a widespread structural motif that is detected in a variety of proteins involved in diversified biological processes. The Ig-fold contains 70-110 residues that are assembled in a characteristic sandwich-like structure formed by two facing β-sheets each made of antiparallel β-strand...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986612802762732
更新日期:2012-10-01 00:00:00
abstract::Human beta-defensin-2 (hBD2) is A small cationic peptide with A broad range of antimicrobial activity. An E. coli cell-free system was employed to express the hBD2 fusion protein by using the hBD2 gene with 14 rare codons. The results showed that the expression level of trxA-hBD2 fusion protein was 0.35 mg/ml, which i...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606775101724
更新日期:2006-01-01 00:00:00
abstract::Apomyoglobin (apoMb) folds through at least two partially folded forms that are detected both as transient intermediates during folding/unfolding kinetics or as stable intermediates at equilibrium. Here, I summarize the results of recent kinetic studies, which combined with detailed characterizations of equilibrium fo...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866053587174
更新日期:2005-04-01 00:00:00
abstract::We report the stepwise transformation of a linear peptide epitope recognized by the anti-transforming growth factor alpha monoclonal antibody Tab2 into peptomers and finally into peptoid analogs. The key experiment in this study is the substitution analysis in which each position of the peptide is exchanged by a set o...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606777841299
更新日期:2006-01-01 00:00:00
abstract::BmK ITa1 cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni(2+)-IDA-sepharose 6B column was toxic for insect, which indicated t...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866023408472
更新日期:2002-10-01 00:00:00
abstract::We examined the production and secretion of fusion constructs containing alpha-N-acetylglucosaminidase, the enzyme deficient in Sanfilippo B, and either wildtype TAT or modified TAT in cultured Spodoptera frugiperda cells. All constructs exhibited successful expression of active enzyme, suggesting the future possibili...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606775974456
更新日期:2006-01-01 00:00:00
abstract::The study of rat proteins is an indispensable task in experimental medicine and drug development. The function of a rat protein is closely related to its subcellular location. Based on the above concept, we construct the benchmark rat proteins dataset and develop a combined approach for predicting the subcellular loca...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611795222768
更新日期:2011-06-01 00:00:00
abstract::After several studies on prediction of mutation, we examine the effect of three sampling strategies, the sampling based on years, the sampling based on number of mutations, and the sampling based on the unpredictable portion of amino-acid pairs, on the prediction performance in H5N1 hemagglutinins. The results show th...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785133735
更新日期:2008-01-01 00:00:00
abstract::We present the results of a study on the influence of albumin and prolactin concentrations and intravascular fluid pH on vein permeability for albumin. Permeability conditions were simulated depending on albumin concentration, pH value and prolactin concentration. In research model an in vitro method was applied using...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/09298665113209990082
更新日期:2014-01-01 00:00:00
abstract::Protein structure information is very useful for the confirmation of protein function. The protein structural class can provide information for protein 3D structure analysis, causing the conformation of the protein overall folding type plays a significant part in molecular biology. In this paper, we focus on the predi...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612803217051
更新日期:2012-11-01 00:00:00
abstract::Successful approaches of de novo protein design suggest a great potential to create novel structural folds and to understand natural rules of protein folding. For these purposes, smaller and simpler de novo proteins have been developed. Here, we constructed smaller proteins by removing the terminal sequences from stab...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986612800494101
更新日期:2012-06-01 00:00:00
abstract::DrTI was effective against trypsin-like enzymes from A. kuehniella and C. cephalonica, however an artificial diet was insufficient to affect the survival and body weight of either insect. The inhibitor stimulated chymotrypsin-like enzymes and probably induced the synthesis of enzymes insensitive to TLCK in neonate lar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986609789839395
更新日期:2009-01-01 00:00:00
abstract::In this paper, a 3D map of protein fold space was produced using Dali structure alignment and nonmetric multidimensional scaling. The fold space comprises four radial clusters, which correspond to the four classes of SCOP. The overall structure of the protein fold space is largely determined by three factors: secondar...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866054395257
更新日期:2005-07-01 00:00:00
abstract::Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866054696163
更新日期:2005-10-01 00:00:00
abstract::Plasmepsin 4 (PM4) is a digestive vacuole enzyme found in all Plasmodium species examined to date. While P. falciparum has three additional aspartic proteinases in its digestive vacuole in addition to plasmepsin 4, other Plasmodium species have only PM4 in their digestive vacuole. Therefore, PM4 may be a good target f...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608785849218
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Peptide: N-glycanase is a deglycosylation enzyme releasing N-glycan from glycoproteins. Although glycan specificity analysis of this enzyme has been reported, recognition requirements for the peptide sequence have not been precisely elucidated. OBJECTIVE:In this study, we carried out peptide specificity ana...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866524666170818160159
更新日期:2017-01-01 00:00:00
abstract::AB204 is an Activin/BMP2 chimera, which has been found to exhibit a higher activity than Bone Morphogenetic Protein 2 (BMP2) in osteogenic activity. To prepare AB204 for its preclinical studies, AB204 has been characterized in various formulation buffers. We observed that AB204 purified by ion-exchange chromatography ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986652105140218110302
更新日期:2014-05-01 00:00:00
abstract::A halophilic nucleoside diphosphate kinase from a moderate halophile, Halomonas sp. 593 (593NDK), was found to be resistant to heat treatment, as indicated by the high level of activity recovery after heating at high temperatures. This is due to reversibility of thermal unfolding, not the high melting temperature, of ...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986606776819628
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Assisted Reproduction Techniques (ART) have significantly advanced since the first successful In Vitro Fertilization (IVF). However, most in vitro-produced embryos fail to implant. Key steps in ART are the correct infertility diagnosis, in order to manage the individualised treatments, and the assessment of ...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866525666180412164602
更新日期:2018-01-01 00:00:00
abstract::Endonuclease tRNase Z catalyzes the generation of the mature 3' end of tRNA precursors through specific endonucleolytic cleavage. The enzyme has been characterized from organisms representative of all domains of life as well as from organelles, and the crystal structure of three bacterial enzymes has been determined. ...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/092986607779816050
更新日期:2007-01-01 00:00:00
abstract::Peptides with more than one biological activity are many a times multifunctional peptides. Two peptides with multifunctional properties from alpha (S2)-casein were stabilized in presence of cosolvents for their biological activities like ACE inhibition activity and antioxidant activity. These bioactive peptides in cos...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986608784246416
更新日期:2008-01-01 00:00:00
abstract::Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986611797642670
更新日期:2011-12-01 00:00:00
abstract::The search for potential inhibitors that target so far unexplored bacterial enzyme mono-N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) has stimulated a development of methodology for quick and efficient preparation of mono-N-acylated 2,6-diaminopimelic acid (DAP) derivatives bearing the different carboxyl gro...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/092986610790780387
更新日期:2010-03-01 00:00:00
abstract::Retro-ACP (74-65) fragment was synthesized on a novel 4% tri-(propylene glycol) glycerolate diacrylate cross-linked polystyrene (PS-TRPGGDA) support. The peptide is grown from the functional site present in the cross-linker, which makes it unique and cost-effective among other styrene based polymer supports. A compara...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866033478753
更新日期:2003-10-01 00:00:00
abstract::In general, halophilic proteins are stable only in the presence of salts at high concentrations. Not only is high salt concentration important for structural stability of halophilic proteins, but also refolding of a denatured halophilic protein requires high salt concentration. This review summarizes the importance of...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866043478220
更新日期:2004-04-01 00:00:00
abstract::Bromelain inhibitor, "bromein", is a proteinase-inhibitor specific to the cysteine proteinase bromelain from pineapple stem. In the stem, eight bromein isoforms are known to exist, and each isoform has a short peptide (light chain) and a long one (heavy chain) with five disulfide bonds. The three-dimensional structure...
journal_title:Protein and peptide letters
pub_type: 杂志文章,评审
doi:10.2174/0929866525666180821115432
更新日期:2018-01-01 00:00:00
abstract::DNA fragmentation factor 45 (DFF45) regulates DNase DFF40 as its inhibitor and chaperone. It was reported that the N-terminal domain (NTD) of DFF45 alone is disordered and DFF40 is necessary as a mutual chaperone for the folding of NTD. However, here we reported the crystallization of DFF45 NTD. These crystals diffrac...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866033479068
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:Recently, the small molecule that inhibits the human copper-trafficking proteins Atox1 and CCS was reported, which suggested that small molecule has an effect on the copper regulation system in the cell. The copper chaperones CopC is regarded as a redox switch and possess barrel structure, thus the interacti...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866526666191204124245
更新日期:2020-01-01 00:00:00
abstract::Supramolecular self-assembled functional materials comprised of cyclic dipeptide building blocks have excellent prospects for biotechnology applications due to their exceptional structural rigidity, morphological flexibility, ease of preparation and modification. Although the pharmacological uses of many natural cycli...
journal_title:Protein and peptide letters
pub_type: 杂志文章
doi:10.2174/0929866527666200212123542
更新日期:2020-01-01 00:00:00