Abstract:
:In the last decade proteomics has made great progress reaching throughput and comprehensiveness comparable to genomics technologies. Mass spectrometry plays a key role in proteomics and has become an indispensable method for molecular and cellular biology because many cellular changes in response to internal or external stimuli can only be detected at the proteome level. Furthermore, different from genomics which depends on the availability of DNA or RNA, proteomics is not restricted to cellular samples, but also allows the analysis of biological fluids like serum, plasma or urine. This article provides an overview of the recent developments in proteomics techniques useful for haematological research.
journal_name
Arch Physiol Biochemjournal_title
Archives of physiology and biochemistryauthors
Fröbel J,Lehr S,Haas R,Czibere Adoi
10.3109/13813450903428086subject
Has Abstractpub_date
2009-12-01 00:00:00pages
286-97issue
5eissn
1381-3455issn
1744-4160journal_volume
115pub_type
杂志文章,评审abstract::Antisense oligonucleotides (ODN) are potent molecules that could be used to inhibit the synthesis of a protein specifically if delivered to the appropriate compartments (cytoplasm and nucleus) of the cell under study. We present here a simple method providing access to the fractions of internalized ODN available in th...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.111.3.265.23465
更新日期:2003-07-01 00:00:00
abstract::Objective: To evaluate the diagnostic and prognostic value of biomarkers and new bioscore in septic patients in intensive care unit (ICU).Materials and methods: A study was conducted on septic and non-septic patients in ICU. Diagnostic and prognostic values of C-reactive protein (CRP), procalcitonin (PCT), Sequential ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1677720
更新日期:2019-11-05 00:00:00
abstract::Circadian rhythms affect a variety of physiological processes. Disruption of circadian rhythms causes many diseases, most of which are associated with inflammation. Disruption of circadian rhythms has a detrimental impact on the function of immune system. It is common to find that circulatory LPS are increased. LPS in...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1782435
更新日期:2020-07-01 00:00:00
abstract::It is known that channel catfish erythrocytes can take up glycine by several distinct transport systems. Further, glycine is an inhibitory neurotransmitter in mammalian brain and spinal cord. Consequently, the uptake of [(3)H]glycine by catfish brain was investigated and found to be a saturable process, dependent on t...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.107.2.84.4349
更新日期:1999-04-01 00:00:00
abstract::Background: We aim to identify and analyze the expression of dyregulated RNAs in colorectal cancer (CRC). Methods: We selected a panel of RNAs specific to CRC composed of Nucleosome Assembly Protein 1 Like 2 (NAP1L2) mRNA, LNCRNA SNHG14 small nucleolar RNA host gene 14 (LNCRNA SNHG14) and homo sapiens microRNA-3940-5p...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1650070
更新日期:2019-08-09 00:00:00
abstract::The dysregulation of microRNA expression is significantly associated with the initiation and development of CRC. miR-124 is markedly downregulated in colorectal cancer. In the present study, the effects of methylation, over expression and downregulation of miR-124 and its target gene DNMT3B on the proliferation, migra...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1779311
更新日期:2020-06-18 00:00:00
abstract::Analysis of miRNAs has a strong potential for the identification of novel prognostic or predictive biomarkers in the serum of AD patients. In this study, we investigated the serum levels of miR-106b as a diagnostic biomarker for AD and evaluate its predictive value for therapeutic response to the drug rivastigmine. Pa...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1734842
更新日期:2020-03-06 00:00:00
abstract:OBJECTIVE:In the present study, we evaluated the effect of biochanin A (BCA) on high-fat diet (HFD)-induced obesity cardiomyopathy. METHODS:BCA (10 mg/kg body weight) was administered to HFD-induced obese rats for 30 days, and its effect on anthropometrical, morphological, plasma cardiac, and inflammatory biomarkers, ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2021.1874017
更新日期:2021-01-20 00:00:00
abstract::The role of adrenoceptor subtypes was studied in rat brown adipose tissue (BAT). The type II 5'-deiodinase (5'DII) was activated in response to simultaneous stimulation by beta3- and alpha1-adrenergic agonists, BRL 37344 or CGP 12177, and cirazoline, in brown adipocytes. Inhibition of the alpha1- and beta-adrenergic p...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813450500500464
更新日期:2006-02-01 00:00:00
abstract::Objective, materials and methods: The aim of this cross-sectional study with a consecutive enrolment was to analyse the role of high sensitive C-reactive protein (hs-CRP) measurement in similarly obese patients with and without the metabolic syndrome (MS). Results: In total, 589 obese patients were screened, of whom 1...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1434207
更新日期:2019-02-01 00:00:00
abstract::This study was carried out to determine a possible relationship between hepatic acetyl-coenzyme A carboxylase EC 6.4.1.2 (ACC) activity in dam and fetus at 15-day and 19-day of gestation and the glucose tolerance in pregnant rats fed on the sucrose diet compared with the rats feed on the dextrin diet. Sucrose feeding ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.105.7.640.11390
更新日期:1997-12-01 00:00:00
abstract::Spirulina platensis, is an alga rich in phycocyanin (potent antioxidant), is effective in regulating the balance of oxidative stress. The objective of this study is to observe the impact of ingestion of a highly oxidised vegetable oil, by rats of Wistar strain. Finally, we observe the effect of Spirulina used as an an...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1444059
更新日期:2019-05-01 00:00:00
abstract::We reasoned that micro-dissection of tumour cells for protein expression studies should be omitted since tumour-stroma interactions are an important part of the biology of solid tumours. To study such interactions in head and neck squamous cell carcinoma (HNSCC) development, we generated reference protein spectra for ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2010.525240
更新日期:2010-10-01 00:00:00
abstract::Aim: This study tested the hypothesis that folic acid (FA) modulates biogenic amines and protects the brain against oxidative stress induced by 3-nitropropionic acid (3NPA).Methods: Male Wistar rats received (groups of six) for 5 d: FA (50 mg/kg); 3NPA (10 mg/kg); or FA +3NPA. At last day, rats were sacrificed, and th...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1484771
更新日期:2020-02-01 00:00:00
abstract::Studies about bone formation and regulation are complex due to a close relationship between bone cells. Primary cell cultures allow to understand osteoblastic function. We isolated cells from the cortical metacarpal bone of 85 or 120 day-old ovine fetuses by an enzymatic method. After first passage and cell amplificat...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.105.2.158.12924
更新日期:1997-04-01 00:00:00
abstract::Evidence is presented of a Mg2+ and Ca(2+)-activated ATPase at the surface of cultured rat glomerular mesangial cells. Substrate specificity was very low when different nucleoside-5'-triphosphates were examined. The apparent Km values were 0.46 and 0.32 mM for Mg(2+)-ATPase and Ca(2+)-ATPase, respectively. Inhibition ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813459509007557
更新日期:1995-04-01 00:00:00
abstract::Background: We investigated whether the anti-inflammatory and antioxidant agent, resveratrol can inhibit type 2 diabetes mellitus (T2DM)-induced osteoarthritis (OA) in rats and whether it is associated with the suppression of glycaemia, dyslipidemia and inflammatory and oxidative stress biomarkers.Materials and method...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1771378
更新日期:2020-06-04 00:00:00
abstract:OBJECTIVE:The potentially beneficial effects of pomegranate peel (PPE), flower (PFE) and seed oil (PSO) extracts, in comparison with rosiglitazone, on adiposity, lipid profile, glucose homoeostasis, as well as on the underlying inflammatory mechanisms, were examined in high-fat and high-sucrose (HF/HS) diet-induced obe...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2016.1148053
更新日期:2016-01-01 00:00:00
abstract::Adipose tissue is not only releasing lipids but also various adipokines that are both dysregulated in the obese state and may contribute to obesity-associated vascular dysfunction and cardiovascular risk. We have previously shown that the combination of adipocyte-conditioned medium (CM) and oleic acid (OA) increases p...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2015.1045520
更新日期:2015-01-01 00:00:00
abstract::Introduction: There are few studies examining the effect of spinosad on cognitive functions in the literature. Methods: In this study, we applied spinosad 3 weeks in different doses to mice and examined their effects on antioxidant system and cognitive performance. Results: In the open field test, we observed a signif...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1623264
更新日期:2019-06-07 00:00:00
abstract::Background: Rheumatoid arthritis (RA) is a joint destructive disorder. This study aimed to assess lncRNA MEG3 expression and its variant rs941576 in Egyptian patients with RA.Subjects and methods: 100 RA patients and 100 healthy individuals were enrolled in the study. Quantitative PCR was used for expression analysis ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1784951
更新日期:2020-07-01 00:00:00
abstract::When neurotransmitters bind to their specific receptors in the membrane of nerve and muscle cells they induce conformational transitions leading to the formation of open receptor-channels and desensitized receptor forms. A knowledge of the rate and equilibrium constants associated with these transitions is required to...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1076/apab.104.6.752.12911
更新日期:1996-10-01 00:00:00
abstract::The effect of underfeeding over glucose metabolism in uteri isolated from ovariectomized and non-ovariectomized rats subjected to a restricted diet for 25 days (50% of the normal food intake), was studied. Underfeeding decreases (14)CO(2) formation from U(14) C-glucose in intact animal uteri. While in ovariectomized r...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.108.5.422.4295
更新日期:2000-12-01 00:00:00
abstract::The influence of ageing on the capacity to increase muscle oxygen delivery during exercise is unclear. This was investigated by comparing the evolution of Near InfraRed Spectroscopy (NIRS) in 10 old (67 +/- 5 years, Old group) and 13 young subjects (27 +/- 4 years, Young group), during a progressive maximal exercise. ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.107.2.159.4343
更新日期:1999-04-01 00:00:00
abstract::To explain the role of the different portions of the stomach in the genesis of electrical signal obtained from abdominal surface (Electrogastrography, EGG), the EGG was recorded in 6 patients with gastric cancer and 7 with duodenal ulcer before and after total gastrectomy or Billroth II gastric resection, respectively...
journal_title:Archives of physiology and biochemistry
pub_type: 临床试验,杂志文章
doi:10.1076/apab.104.1.50.12867
更新日期:1996-01-01 00:00:00
abstract::This study was to investigate whether epidermal growth factor (EGF) may induce any long-term effect on pancreatic exocrine function in vivo as well to evaluate the chronic effects of EGF on pancreatic growth in rats. Rats were treated with EGF (10 micrograms/kg) for 5 or 7 days. EGF infused intravenously (2 micrograms...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.104.3.293.12909
更新日期:1996-01-01 00:00:00
abstract::Diabetic cardiomyopathy consists of a series of structural and functional changes. Accumulating evidence supports the concept that a "cardiac stem cell compartment disease" plays an important role in the pathophysiology of diabetic cardiomyopathy. In diabetic hearts, human cardiac stem/progenitor cells (CSPC) are redu...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.3109/13813455.2013.798334
更新日期:2013-10-01 00:00:00
abstract::Pulmonary ischemia-reperfusion (IR) injury may result from trauma, atherosclerosis, pulmonary embolism, pulmonary thrombosis and surgical procedures such as cardiopulmonary bypass and lung transplantation. IR injury induces oxidative stress characterized by formation of reactive oxygen (ROS) and reactive nitrogen spec...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1080/13813450601118976
更新日期:2007-02-01 00:00:00
abstract::Phospholipid fatty acid composition and bilirubin UDP-glucuronyltransferase activity from liver microsomal membrane were studied in normal and in bile duct ligated rats. Incubation of normal microsomes with 15 microM bilirubin (considered as physiological concentration) yielded 60% bilirubin diglucuronide; in 2 days p...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813459509047136
更新日期:1995-08-01 00:00:00
abstract::Several studies have suggested that fibroblast-like synoviocytes (FLSs) and miRNAs are implicated in the pathogenesis of rheumatoid arthritis (RA). This study was aimed to evaluate the function of miR-6089 in the regulation of RA-FLSs. The levels of miR-6089 were detected to be significantly lower in the synovial tiss...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1773862
更新日期:2020-06-17 00:00:00