Uptake of [(3)H]glycine by synaptosomes of channel.

Abstract:

:It is known that channel catfish erythrocytes can take up glycine by several distinct transport systems. Further, glycine is an inhibitory neurotransmitter in mammalian brain and spinal cord. Consequently, the uptake of [(3)H]glycine by catfish brain was investigated and found to be a saturable process, dependent on the presence of Na(++) and Cl(--) and sensitive to temperature. A kinetic analysis of transport was performed at 22C. This showed that a high-affinity system existed which exhibited a K(m) of 5.1 (+/- 2. 1) microM. Several structural analogues of glycine were capable of inhibiting uptake in a competitive manner. The most effective inhibitor was sarcosine (IC(50) 5 36 microM). Uptake was also able to be inhibited by harmaline, a drug known to interfere with Na(+)-dependent transport processes. It is concluded that glycine transport by channel catfish brain has much in common with transport by mammalian nervous tissue which is carried out by the membrane carriers GLYT1 and GLYT2. On the other hand, synaptosomal transport differs somewhat from glycine transport by channel catfish erythrocytes.

journal_name

Arch Physiol Biochem

authors

Wolf TR,Lance RA,Hart ER,Tunnicliff G

doi

10.1076/apab.107.2.84.4349

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

84-90

issue

2

eissn

1381-3455

issn

1744-4160

journal_volume

107

pub_type

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