Expression of metallothioneins I and II related to oxidative stress in the liver of aluminium-treated rats.

Abstract:

:Hepatotoxicity, induced by aluminium chloride (AlCl3), has been well studied but there are no reports about liver metallothionein (MT) genes induction. Therefore, it is of interest to establish the mechanism involving the relation between MT gene expression levels and the oxidative stress status in hepatic cells of aluminium-treated rats. Aluminium (Al) was administered to rats in their drinking water at a dose of 50 mg/kg body weight for three weeks. AlCl3 provoked hepatotoxicity objectified by an increase in malondialdehyde (MDA), hydrogen peroxide (H2O2), advanced oxidation protein products (AOPP), protein carbonyls (PCO) and a decrease in reduced glutathione (GSH), non-protein thiols (NPSH) and vitamin C. CAT and Glutathione peroxidase (GPx) activities were decreased while Mn-SOD gene expression, total Metallothionein content and MT I and MT II genes induction were increased. There are changes in plasma of some trace elements, albumin levels, transaminases, LDH and ALP activities. All these changes were supported by histopathological observations.

journal_name

Arch Physiol Biochem

authors

Ghorbel I,Chaabane M,Elwej A,Boudawara O,Abdelhedi S,Jamoussi K,Boudawara T,Zeghal N

doi

10.1080/13813455.2016.1187176

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

214-222

issue

4

eissn

1381-3455

issn

1744-4160

journal_volume

122

pub_type

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