Abstract:
:Separating proteins according to two different gel-electrophoretic methods not only increases the resolution power for highly complex samples when compared to one-dimensional separations, but is also a valuable tool for protein and protein complex characterization. There are a number of different electrophoresis methods which can be combined. The combination of isoelectric focusing under denaturing conditions and SDS polyacrylamide gel electrophoresis delivers the highest resolution of all bio-analytic techniques. This is a short review on the history and state of the art of two-dimensional electrophoresis methods, and contains some practical tips for high resolution 2D electrophoresis, which are based on several decades of experience with this method.
journal_name
Arch Physiol Biochemjournal_title
Archives of physiology and biochemistryauthors
Westermeier Rdoi
10.3109/13813455.2014.945188subject
Has Abstractpub_date
2014-12-01 00:00:00pages
168-72issue
5eissn
1381-3455issn
1744-4160journal_volume
120pub_type
杂志文章,评审abstract::When neurotransmitters bind to their specific receptors in the membrane of nerve and muscle cells they induce conformational transitions leading to the formation of open receptor-channels and desensitized receptor forms. A knowledge of the rate and equilibrium constants associated with these transitions is required to...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1076/apab.104.6.752.12911
更新日期:1996-10-01 00:00:00
abstract::Glycogen synthesis increases after muscle contraction and during insulin stimulation, and insulin-stimulated glycogen synthesis is enhanced after contraction. We hypothesized that the initial glycogen content influences the magnitude of additive effect of contraction on insulin-stimulated glycogen synthesis. Contracti...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2010.494670
更新日期:2010-07-01 00:00:00
abstract:CONTEXT:Mechanisms by which ghrelin affords its cardioprotection in mammals remained unclear. OBJECTIVE:To examine if ghrelin confers cardio-protection during cardiac remodelling post-MI by modulating the RAF-1-MEK1/2-ERK1/2 signalling pathway. MATERIALS AND METHODS:Rats were divided into control, sham, sham + ghreli...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1437751
更新日期:2019-05-01 00:00:00
abstract::Objectives: The main aim of this study was to investigate the effects of irisin on asprosin, leptin, glucose levels and lipid profile in healthy and obese male and female rats.Methods: Irisin was subcutaneously administered with osmotic minipumps at the dose of 100 ng/kg/day for 28 days and then, the serum levels of a...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1722706
更新日期:2020-02-06 00:00:00
abstract::To explain the role of the different portions of the stomach in the genesis of electrical signal obtained from abdominal surface (Electrogastrography, EGG), the EGG was recorded in 6 patients with gastric cancer and 7 with duodenal ulcer before and after total gastrectomy or Billroth II gastric resection, respectively...
journal_title:Archives of physiology and biochemistry
pub_type: 临床试验,杂志文章
doi:10.1076/apab.104.1.50.12867
更新日期:1996-01-01 00:00:00
abstract::The mechanism underlying the increased rate of endogenous glucose production from the liver during exercise remains unknown. The cytokine interleukin-6 (IL-6) is known to be released during exercise and is thought that either IL-6 directly or via a "contraction factor" stimulates the release of stored glucose from the...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2016.1146773
更新日期:2016-01-01 00:00:00
abstract::An accumulating body of evidence supports the role of autophagy in the pathophysiology of T2DM. Also, abnormal endoplasmic reticulum (ER) stress response that has been implicated as a cause of insulin resistance (IR) could also be affected by the autophagic status in β-cells. The present study was designed to investig...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1628069
更新日期:2019-06-19 00:00:00
abstract::The effect of underfeeding over glucose metabolism in uteri isolated from ovariectomized and non-ovariectomized rats subjected to a restricted diet for 25 days (50% of the normal food intake), was studied. Underfeeding decreases (14)CO(2) formation from U(14) C-glucose in intact animal uteri. While in ovariectomized r...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.108.5.422.4295
更新日期:2000-12-01 00:00:00
abstract::This study was to investigate whether epidermal growth factor (EGF) may induce any long-term effect on pancreatic exocrine function in vivo as well to evaluate the chronic effects of EGF on pancreatic growth in rats. Rats were treated with EGF (10 micrograms/kg) for 5 or 7 days. EGF infused intravenously (2 micrograms...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.104.3.293.12909
更新日期:1996-01-01 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is the most prevalent disease and becoming a serious public health threat worldwide. In recent years, numerous effective T2DM intervention regimens have been developed, with promising results. However, these regimens are not usually economically available, and they are not well tolerate...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1657899
更新日期:2019-09-02 00:00:00
abstract::Adipose tissue is not only releasing lipids but also various adipokines that are both dysregulated in the obese state and may contribute to obesity-associated vascular dysfunction and cardiovascular risk. We have previously shown that the combination of adipocyte-conditioned medium (CM) and oleic acid (OA) increases p...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2015.1045520
更新日期:2015-01-01 00:00:00
abstract:CONTEXT:Cardiometabolic disorders are rapidly becoming major public health challenges. Valproic acid (VPA) is a widely prescribed anticonvulsant drug. OBJECTIVE:We hypothesized that treatment with VPA would improve the regulation of glucose and atherogenic dyslipidemia through reduction in circulating corticosterone. ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2016.1151892
更新日期:2016-07-01 00:00:00
abstract::The consumption of high calorie-content diets is the first cause of obesity, probably the main health issue worldwide; however, the experimental evidences for evaluating the differential metabolic modifications of high-sucrose or high-fat diets are scare. We evaluated the metabolic outcomes of the obesity induced by t...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1517181
更新日期:2020-07-01 00:00:00
abstract::The roles of leptin, nesfatin-1 and kisspeptin in the regulation of food intake and/or reproduction are well known; however, the interactions between these hormones remain unclear, especially in humans. The aim of this study was to determine the roles of leptin, nesfatin-1 and kisspeptin in pre- and postmenopausal obe...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2016.1171365
更新日期:2016-10-01 00:00:00
abstract::The G0/G1 switch gene 2 (G0S2) protein attenuated adipose triglyceride lipase (ATGL) activity and decreased lipolysis in rodent and human adipocytes. We hypothesized that G0S2 mRNA expression in human adipose tissue is influenced by depot, adipocyte size, body weight and caloric intake. Adipose tissue samples were obt...
journal_title:Archives of physiology and biochemistry
pub_type: 临床试验,杂志文章
doi:10.3109/13813455.2015.1122066
更新日期:2016-01-01 00:00:00
abstract::Background: The microRNAs have come up as crucial mediators of energy balance and metabolic control. CD36 is potential biomarker of obesity and metabolic syndrome. This study investigates the concentration of miR-146a and miR-21 and CD 36 in blood samples of obese and healthy young participants. We assessed the associ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1775655
更新日期:2020-06-13 00:00:00
abstract::Diabetes is associated with depression and anxiety symptoms. The current investigation was designed to explore the effect of melatonin on depressive and anxiety like-behaviours, oxidative stress, levels of AGE, RAGE and S100B in streptozotocin-induced diabetic rats. The animals were divided into four groups: Normoglyc...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1684954
更新日期:2019-11-15 00:00:00
abstract::The role of adrenoceptor subtypes was studied in rat brown adipose tissue (BAT). The type II 5'-deiodinase (5'DII) was activated in response to simultaneous stimulation by beta3- and alpha1-adrenergic agonists, BRL 37344 or CGP 12177, and cirazoline, in brown adipocytes. Inhibition of the alpha1- and beta-adrenergic p...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813450500500464
更新日期:2006-02-01 00:00:00
abstract:AIM:A high-fat diet (HFD) can lead to obesity and related metabolic disorders. This study evaluated the preventive efficacy of myricetin derivative-rich fraction (MD) from Syzygium malaccense leaf extract against HFD-induced obesity, hyperglycaemia, and oxidative stress in C57BL/6J mice. METHODS:HFD-fed mice were admi...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1808019
更新日期:2020-08-19 00:00:00
abstract::The effectiveness of betulinic acid (B) and PLGA loaded nanoparticles of B (Bnp) against hepatocellular carcinoma (HCC) was established and reported earlier. In continuation of our previous report, the present study described the molecular mechanisms of their antineoplastic responses. In this context, the antineoplast...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1733024
更新日期:2020-03-06 00:00:00
abstract::Context: Recent surveys have shown an association between proprotein convertase subtilisin/kexin type 9 (PCSK9) and oxidative stress.Objective: In this investigation, the effect of evolocumab an anti-PCSK9 antibody was assessed against oxidative damage caused by hydrogen peroxide (H2O2) in human umbilical vein endothe...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1788605
更新日期:2020-07-03 00:00:00
abstract::The transport system for the cytokine tumor necrosis factor-alpha (TNFalpha) at the blood-brain barrier (BBB) enables an enhanced yet saturable entry of TNFalpha from blood to the CNS. This review focuses on the selective upregulation of the transport system for TNFalpha at the BBB that is specific for type of patholo...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1076/apab.109.4.350.4238
更新日期:2001-10-01 00:00:00
abstract::Objective: To evaluate the diagnostic and prognostic value of biomarkers and new bioscore in septic patients in intensive care unit (ICU).Materials and methods: A study was conducted on septic and non-septic patients in ICU. Diagnostic and prognostic values of C-reactive protein (CRP), procalcitonin (PCT), Sequential ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1677720
更新日期:2019-11-05 00:00:00
abstract::Serum uric acid is associated with an increased risk of hypertension, cardiovascular disease and chronic kidney disease. Hyperuricemia raises the risk of insulin resistance and metabolic syndrome including diabetes. Aim: To find the association between purine metabolites and diabetic complications in rats. Materials a...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1663219
更新日期:2019-09-13 00:00:00
abstract::This study investigates the effects of the islet hormones insulin (Ins), glucagon (Glu), and somatostatin (Som) with nerve stimulation (EFS) acetylcholine (ACh) and cholecytokinin-octapeptide (CCK-8) on amylase secretion and intracellular free calcium concentration [Ca(2+)](i) in the pancreas of age-matched control an...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.109.3.252.11585
更新日期:2001-07-01 00:00:00
abstract::Objective: The pattern of urinary excretion of total sulphated glycosaminoglycans (GAGs) and their particular types: chondroitin sulphate/dermatan sulphate (CS/DS) and heparan sulphate (HS) was analysed in obese patients with type 2 diabetes mellitus (T2DM) treated with metformin in monotherapy for the period of six m...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1697889
更新日期:2019-12-09 00:00:00
abstract::Antisense oligonucleotides (ODN) are potent molecules that could be used to inhibit the synthesis of a protein specifically if delivered to the appropriate compartments (cytoplasm and nucleus) of the cell under study. We present here a simple method providing access to the fractions of internalized ODN available in th...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.111.3.265.23465
更新日期:2003-07-01 00:00:00
abstract::We reasoned that micro-dissection of tumour cells for protein expression studies should be omitted since tumour-stroma interactions are an important part of the biology of solid tumours. To study such interactions in head and neck squamous cell carcinoma (HNSCC) development, we generated reference protein spectra for ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2010.525240
更新日期:2010-10-01 00:00:00
abstract::Diabetes mellitus, especially when complicated with decline of renal function due to diabetic nephropathy (DN), is associated with accumulation of advanced glycation end products (AGEs) exerting their adverse effects via receptor of AGE (RAGE). Soluble RAGE (sRAGE) is a truncated form of RAGE functioning as an inhibit...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813450802033818
更新日期:2008-04-01 00:00:00
abstract::In the suckling and adult rats equilibrated or no by 125I, cutaneous iodine analysed by dialysis and chromatography techniques (Dowex and Sephadex) was the purpose of this study. Dialysis studies had shown that most of steady or labelled skin iodine had an iodide form (90 to 95% of the total iodine). There were at lea...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813459509047146
更新日期:1995-08-01 00:00:00