Aberrant methylation of miR-124 upregulates DNMT3B in colorectal cancer to accelerate invasion and migration.

Abstract:

:The dysregulation of microRNA expression is significantly associated with the initiation and development of CRC. miR-124 is markedly downregulated in colorectal cancer. In the present study, the effects of methylation, over expression and downregulation of miR-124 and its target gene DNMT3B on the proliferation, migration and invasion of colorectal cell line were investigated. The promoter methylation status of miR-124 in the CRC was investigated by methylation specific PCR (MSP). The potential role of miR-124 expression in CRC cells was investigated using the demethylation reagent 5-Aza-CdR and transfection of miR-124 mimic/antimir. MSP revealed that miR-124 promoter region was hypermethylated, result in its significant downregulation in tumour tissues. We showed miR-124 expression was upregulated following 5-AZA-CdR treatment. Transfected Hct-116 cell line with miR-124 leads to decreased DNMT3B expression, cell proliferation, migration and invasion of HCT-116. In conclusion, our data indicate that miR-124 suppress colorectal cancer proliferation, migration and invasion through downregulating DNMT3B level.

journal_name

Arch Physiol Biochem

authors

Shahmohamadnejad S,Nouri Ghonbalani Z,Tahbazlahafi B,Panahi G,Meshkani R,Emami Razavi A,Shokri Afra H,Khalili E

doi

10.1080/13813455.2020.1779311

subject

Has Abstract

pub_date

2020-06-18 00:00:00

pages

1-7

eissn

1381-3455

issn

1744-4160

pub_type

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