Abstract:
:Isolation of glycosylated 26 kDa rat prolactin and subsequent proper carbohydrate characterization has so far not been reported. In the present work the hormone isoform was isolated to 95% homogeneity by preparative electrophoretic separation on Mini Prep Cell of rat pituitary homogenate. The isoform was then investigated by 2-mercaptoethanol gradient electrophoresis, Cleveland's sequential SDS-PAGE, digestion with endoproteinase Asp-N and N-glycanase. The glycosidic part of the isoform was examined in O-profiling and its monosaccharide composition obtained by FACE and HPAE-PAD analysis. The outcome of the experimental data is: 1) in contrast to unglycosylated 23 kDa rat prolactin, intra-chain S-S bridging is not affected in 26kDa rat prolactin, neither by transiting through a thiol gradient nor in sequential nonreducing/reducing SDS-PAGE; 2) the conformational availability of Asp residues involved in the endoproteinase Asp-N attack is the same in 23- and 26 kDa rat prolactin; the glycan moiety apparently does not cause steric hindrance at this level; 3) no glycosidic N-linkage could be detected, only O-linkage(s); 4) 26 kDa rat prolactin is no glycosyl-phosphaditylinositol-anchored protein; 5) in O-profiling an oligosaccharide chain of Mr +/- 1.4 kDa was recorded; 6) the monosaccharide composition obtained in FACE is peculiar in the sense that next to Fuc, Man, GalNac, GlcNac and NeuAc also Rib was determined; 7) HPAE-PAD analysis identified NeuAc subtypes; 8) in vitro, glycosylation of rat prolactin modulates immune recognition through steric hindrance of the access to the epitope sites.
journal_name
Arch Physiol Biochemjournal_title
Archives of physiology and biochemistryauthors
Bollengier F,Mahler A,Braet C,Claeyssens M,Vanhaelst Ldoi
10.1076/apab.109.2.180.4276subject
Has Abstractpub_date
2001-04-01 00:00:00pages
180-90issue
2eissn
1381-3455issn
1744-4160journal_volume
109pub_type
杂志文章abstract::This study investigates the alcoholic extract effect of Alhagi camelorum on blood glucose and lipid profiles in diabetic rats made by streptozotocin (STZ). Male rats were divided into four groups. The control group received a normal diet. The diabetic group was induced by STZ and two diabetic experimental groups recei...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1635623
更新日期:2019-08-04 00:00:00
abstract::Objective: The present study aimed to investigate the effects of Juglans regia (JR) extract on hyperglycaemia and insulin sensitivity. Methods: Forty rats were divided into 4 groups: Control (C), Diabetes Control (DC), Diabetes + Juglans regia (D + JR) and Diabetes + Metformin (D + M). Results: In the D + JR group, Fa...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1668018
更新日期:2019-09-23 00:00:00
abstract:OBJECTIVE:The aim was to determine adenosine deaminase (ADA) and alkaline phosphatase (ALP) activity in sputum for the diagnosis of pulmonary tuberculosis. METHODS:This study comprised of 64 subjects (33 pulmonary tuberculosis and 31 lung cancer). The ADA and ALP activity was measured in sputum and serum. RESULTS:The...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2011.645545
更新日期:2012-02-01 00:00:00
abstract:CONTEXT:The views of type 2 diabetes (T2DM) patients have not been considered in the debate on the role of self-monitoring of blood glucose (SMBG) in the management of T2DM. OBJECTIVE:To assess the views of T2DM patients on SMBG. METHODS:Two previously trained research assistants used a structured pre-tested question...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2011.625950
更新日期:2012-02-01 00:00:00
abstract::It is known that oxidative stress plays an important role in the chronic complications of diabetes. Lipid peroxidation is one of the consequences of oxidative stress. Erythrocyte deformability abilities are reduced as a result of lipid peroxidation. Conversely, a decrease nitric oxide (NO) production seems to be respo...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2012.714790
更新日期:2012-12-01 00:00:00
abstract::Type 2 Diabetes mellitus (T2D) is the most common endocrine disorder associated to metabolic syndrome (MS) and occurs when insulin secretion can no compensate peripheral insulin resistance. Among peripheral tissues, the liver controls glucose homeostasis due to its ability to consume and produce glucose. The molecular...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.3109/13813455.2011.557386
更新日期:2011-07-01 00:00:00
abstract::The glucose-fatty acid cycle as proposed four decades ago by Randle suggests that insulin resistance develops in consequence of alterations of the metabolic pressure of lipids. The more recently published 'hexosamine pathway theory' and the 'malonyl-CoA hypothesis' depict insulin resistance as a consequence of an imba...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.1076/apab.109.1.38.4284
更新日期:2001-02-01 00:00:00
abstract::Context: The anti-diabetic medicine metformin has been reported as an anti-ageing drug candidate as it mimics the benefits of caloric restriction and reduces ageing-related oxidative stress in various experimental organisms. Objective: We investigated the possible anti-oxidative role of metformin against rotenone-indu...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1620288
更新日期:2019-06-01 00:00:00
abstract::Objective: To explore the probable in vitro, in situ and in vivo mechanisms of gallic acid (GA) and p-coumaric acid (PCA) as anti-hyperglycemic agents.Animals and methods: Male albino rats were allocated into four groups, group1 was used as normal control. Group 2 was established as a diabetic control and group3 and 4...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1762659
更新日期:2020-05-13 00:00:00
abstract::Context: Recent surveys have shown an association between proprotein convertase subtilisin/kexin type 9 (PCSK9) and oxidative stress.Objective: In this investigation, the effect of evolocumab an anti-PCSK9 antibody was assessed against oxidative damage caused by hydrogen peroxide (H2O2) in human umbilical vein endothe...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1788605
更新日期:2020-07-03 00:00:00
abstract::This study was to investigate whether epidermal growth factor (EGF) may induce any long-term effect on pancreatic exocrine function in vivo as well to evaluate the chronic effects of EGF on pancreatic growth in rats. Rats were treated with EGF (10 micrograms/kg) for 5 or 7 days. EGF infused intravenously (2 micrograms...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.104.3.293.12909
更新日期:1996-01-01 00:00:00
abstract:CONTEXT:Glucose-6-phosphate dehydrogenase (G6PD) is an important enzyme of hexose monophosphate shunt, involved in the biosynthesis of reduced nicotinamide adenine dinucleotide phosphate hydrogen (NADPH). OBJECTIVE:This study was designed to investigate age-dependent changes in human erythrocyte G6PD activity. The G6P...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2015.1136648
更新日期:2016-01-01 00:00:00
abstract::Autophagy is an important life phenomenon in eukaryotic cells. Its main role is to remove and degrade its damaged organelles and excess biological macromolecules, and use degradation products to provide energy and rebuild the cell structure, playing an important role in maintaining cell homeostasis and cell life activ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1675714
更新日期:2019-10-09 00:00:00
abstract::Studies about bone formation and regulation are complex due to a close relationship between bone cells. Primary cell cultures allow to understand osteoblastic function. We isolated cells from the cortical metacarpal bone of 85 or 120 day-old ovine fetuses by an enzymatic method. After first passage and cell amplificat...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.105.2.158.12924
更新日期:1997-04-01 00:00:00
abstract:OBJECTIVE:To assess the relationship of serum osteocalcin levels with blood glucose, insulin resistance and lipid profile in central Indian men with type 2 diabetes. METHODS:Anthropometric and biochemical parameters were measured in 56 newly diagnosed type 2 diabetic men and in 56 age and body mass index (BMI) matched...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2012.715651
更新日期:2012-12-01 00:00:00
abstract::An experiment in which the rats access either to 0.5% or 1.5% saline was designed in order to further characterise the relationship between sodium intake and renal excretion after acute immobilization stress. A saline solution for 3 days was provided to the rats previous to the experimental day. On that day, after fin...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/1381345520000710831zft268
更新日期:2000-07-01 00:00:00
abstract::Several studies have suggested that fibroblast-like synoviocytes (FLSs) and miRNAs are implicated in the pathogenesis of rheumatoid arthritis (RA). This study was aimed to evaluate the function of miR-6089 in the regulation of RA-FLSs. The levels of miR-6089 were detected to be significantly lower in the synovial tiss...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1773862
更新日期:2020-06-17 00:00:00
abstract::Mammalian gonadotropin-releasing hormone (GnRH I) is a hypothalamic decapeptide that governs gonadotropin secretion through interaction with its seven transmembrane (7TM), G protein-coupled receptor (GPCR) expressed by anterior pituitary cells. A second decapeptide, GnRH II, originally discovered in the chicken hypoth...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.110.1.129.900
更新日期:2002-04-01 00:00:00
abstract:CONTEXT:The important role of exercise in pulmonary function during menopause is not well known. Oestrogen deficiency in ageing female mice is accompanied by increase in apoptotic markers such as caspase3 in the lung. OBJECTIVE:The present study was designed to investigate whether swimming training will ameliorate fib...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2017.1396347
更新日期:2018-10-01 00:00:00
abstract::Background: Oxidative stress, has been shown to play an important role in the pathophysiology of cardiac remodelling and heart failure. The aim of study is effect of arginine vasopressin (AVP) on apoptosis of cardiomyocyte via its receptors.Materials and methods: The cell viability effect of AVP in H9C2 cardiomyocytes...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1729816
更新日期:2020-03-06 00:00:00
abstract::To investigate the antidiabetic effect of isopulegol in high-fat diet (HFD)/streptozotocin (STZ)-induced diabetic rats. Animals were made diabetic by feeding HFD for 4 weeks followed by single intraperitoneal injection of STZ (35 mg/kg b.w.; 0.1 M citrate buffer; pH 4.0). Plasma insulin, haemoglobin and glycogen conte...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1638415
更新日期:2019-07-10 00:00:00
abstract::Adipose tissue is not only releasing lipids but also various adipokines that are both dysregulated in the obese state and may contribute to obesity-associated vascular dysfunction and cardiovascular risk. We have previously shown that the combination of adipocyte-conditioned medium (CM) and oleic acid (OA) increases p...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2015.1045520
更新日期:2015-01-01 00:00:00
abstract::The early and late effects of alloxan-diabetes and insulin treatment on kinetic properties of mitochondrial FoF1 ATPase were examined. Diabetic state resulted in significant decrease in the activity while insulin treatment caused hyper-stimulation. In control animals the enzyme activity resolved in three kinetic compo...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813450600935206
更新日期:2006-07-01 00:00:00
abstract:OBJECTIVE:To determine the relationship of biochemical parameters, BMI and blood pressure with age, gender and ethnicity of Trinidadian type 2-diabetic subjects. METHODS:A retrospective case control study comprised of 1794 patient (740 males and 1054 females). RESULTS:The correlation was found for lipid profile with ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2011.613482
更新日期:2012-02-01 00:00:00
abstract::This present study investigated the antioxidative and antidiabetic properties of kolaviron by analysing its inhibitory effect on key metabolic activities linked to T2D, in vitro and ex vivo. Kolaviron significantly inhibited α-glucosidase and α-amylase activities, and intestinal glucose absorption dose-dependently, wh...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1806331
更新日期:2020-08-17 00:00:00
abstract::Hepatotoxicity, induced by aluminium chloride (AlCl3), has been well studied but there are no reports about liver metallothionein (MT) genes induction. Therefore, it is of interest to establish the mechanism involving the relation between MT gene expression levels and the oxidative stress status in hepatic cells of al...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2016.1187176
更新日期:2016-10-01 00:00:00
abstract::Diabetes mellitus, especially when complicated with decline of renal function due to diabetic nephropathy (DN), is associated with accumulation of advanced glycation end products (AGEs) exerting their adverse effects via receptor of AGE (RAGE). Soluble RAGE (sRAGE) is a truncated form of RAGE functioning as an inhibit...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813450802033818
更新日期:2008-04-01 00:00:00
abstract::An enhanced luminescence technique was used to monitor the response of liver homogenates stressed with sodium perborate. Rat liver homogenates were subjected to oxidative stress with sodium perborate, and the light signals, generated by a suitable system, containing luminol and compounds producing enhancement of light...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813459508996132
更新日期:1995-05-01 00:00:00
abstract::For the identification of proteins from biological samples by mass spectrometry there are two scenarios. Proteins are pre-fractionated in some way or are analysed as a complex mixture (shotgun). Shotgun proteomics recently became more popular, because of technological developments on the mass spectrometer side which n...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.3109/13813455.2014.955032
更新日期:2014-12-01 00:00:00
abstract::Dopamine (DA) is thought to modulate the transduction of the hypoxic stimulus by the glomus cell in the carotid body (CB). The hypothesis tested here is that presynaptic DA D2 receptors (D2's) located on the type 1 cell function as autoreceptors to control DA release and/or synthesis. The aim of the study was to compa...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.105.1.3.13145
更新日期:1997-02-01 00:00:00