Insulin-status-dependent modulation of FoF1 ATPase activity in rat kidney mitochondria.

Abstract:

:The early and late effects of alloxan-diabetes and insulin treatment on kinetic properties of mitochondrial FoF1 ATPase were examined. Diabetic state resulted in significant decrease in the activity while insulin treatment caused hyper-stimulation. In control animals the enzyme activity resolved in three kinetic components. In diabetic condition only component I and II were present. With insulin treatment component III was restored but component II was abolished. Diabetic state and insulin treatment had varied effects on Km values of the three components, whereas the Vmax values were generally on the higher side. Evaluation of the AppKcat/Km values revealed that diabetic state resulted in increased catalytic efficiency; insulin treatment brought back these values to normality. Temperature kinetics studies indicated that the phase transition temperature decreased significantly in the diabetic and insulin-treated diabetic animals. The energy of activation in low temperature range increased in the diabetic animals. Insulin treatment corrected the Arrhenius pattern at early stage of diabetes; at late stage the pattern was reversed. The results are suggestive of subtle insulin-status-dependent alterations in membrane structure - function relationships.

journal_name

Arch Physiol Biochem

authors

Patel SP,Katyare SS

doi

10.1080/13813450600935206

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

150-7

issue

3

eissn

1381-3455

issn

1744-4160

pii

JP2717L056G41710

journal_volume

112

pub_type

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