Effects of starvation, diabetes and carbon tetrachloride intoxication on rat kidney cortex and liver pyruvate carboxylase levels.

Abstract:

:Pyruvate carboxylase (PC) has been quantified in rat liver and kidney cortex under experimental conditions that modify the gluconeogenic response in both organs: fasting, carbon tetrachloride-induced liver degeneration and alloxan-induced diabetes. Enzymatic activity has been assayed by a 14CO2-fixation method. The amount of enzyme has been determined by competitive ELISA using antibodies raised against the purified rat kidney cortex enzyme. Purified fractions of rat-liver and rat-kidney cortex PC have been used as standards. Fasting and carbon tetrachloride administration induced a significant increase (25% to 30%) in the amount of enzyme in liver and kidney cortex. Alloxan-induced diabetes produced a nearly two-fold increase in the hepatic levels of enzyme without a significant modification in the content of the renal enzyme. These results are discussed on the basis of the different metabolic implications of both organs during the physiological or toxic treatments.

journal_name

Arch Physiol Biochem

authors

Salto R,Sola M,Oliver FJ,Vargas AM

doi

10.1076/apab.104.7.845.13111

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

845-50

issue

7

eissn

1381-3455

issn

1744-4160

journal_volume

104

pub_type

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