Determination of the chemical mechanism of neurotransmitter receptor-mediated reactions by rapid chemical kinetic methods.

Abstract:

:When neurotransmitters bind to their specific receptors in the membrane of nerve and muscle cells they induce conformational transitions leading to the formation of open receptor-channels and desensitized receptor forms. A knowledge of the rate and equilibrium constants associated with these transitions is required to (i) relate the mechanism of the receptor-mediated reaction to the resulting changes in transmembrane voltage that trigger signal transmission between neurons, (ii) calculate changes in transmembrane voltage that result from the interaction of diverse excitatory and inhibitory receptors in the same cell, and (iii) understand the mechanism by which receptor function is affected by activators, inhibitors, including clinically important compounds, and diseases of the nervous system. The conformational transitions of interest occur in the millisecond and the sub-millisecond time region. Chemical kinetic techniques for studying reactions mediated by membrane-bound neurotransmitter receptors in cells or vesicles in this time domain were not available. Here we describe the development and use of a laser pulse photolysis technique suitable for chemical kinetic investigations of neurotransmitter receptors in the mu s and ms time region. The type of information that can be obtained is also discussed.

journal_name

Arch Physiol Biochem

authors

Hess GP

doi

10.1076/apab.104.6.752.12911

subject

Has Abstract

pub_date

1996-10-01 00:00:00

pages

752-61

issue

6

eissn

1381-3455

issn

1744-4160

journal_volume

104

pub_type

杂志文章,评审
  • Sputum adenosine deaminase and alkaline phosphatase activity in pulmonary tuberculosis.

    abstract:OBJECTIVE:The aim was to determine adenosine deaminase (ADA) and alkaline phosphatase (ALP) activity in sputum for the diagnosis of pulmonary tuberculosis. METHODS:This study comprised of 64 subjects (33 pulmonary tuberculosis and 31 lung cancer). The ADA and ALP activity was measured in sputum and serum. RESULTS:The...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.3109/13813455.2011.645545

    authors: Jadhav AA,Jain A

    更新日期:2012-02-01 00:00:00

  • Regulation of G0/G1 switch gene 2 (G0S2) expression in human adipose tissue.

    abstract::The G0/G1 switch gene 2 (G0S2) protein attenuated adipose triglyceride lipase (ATGL) activity and decreased lipolysis in rodent and human adipocytes. We hypothesized that G0S2 mRNA expression in human adipose tissue is influenced by depot, adipocyte size, body weight and caloric intake. Adipose tissue samples were obt...

    journal_title:Archives of physiology and biochemistry

    pub_type: 临床试验,杂志文章

    doi:10.3109/13813455.2015.1122066

    authors: Skopp A,May M,Janke J,Kielstein H,Wunder R,Flade-Kuthe R,Kuthe A,Jordan J,Engeli S

    更新日期:2016-01-01 00:00:00

  • Circulating mir-21 and mir-146a are associated with increased cytokines and CD36 in Algerian obese male participants.

    abstract::Background: The microRNAs have come up as crucial mediators of energy balance and metabolic control. CD36 is potential biomarker of obesity and metabolic syndrome. This study investigates the concentration of miR-146a and miR-21 and CD 36 in blood samples of obese and healthy young participants. We assessed the associ...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1775655

    authors: Benbaibeche H,Hichami A,Oudjit B,Haffaf EM,Kacimi G,Koceïr EA,Khan NA

    更新日期:2020-06-13 00:00:00

  • Secretory activity and trophic effects of epidermal growth factor in the rat pancreas.

    abstract::This study was to investigate whether epidermal growth factor (EGF) may induce any long-term effect on pancreatic exocrine function in vivo as well to evaluate the chronic effects of EGF on pancreatic growth in rats. Rats were treated with EGF (10 micrograms/kg) for 5 or 7 days. EGF infused intravenously (2 micrograms...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1076/apab.104.3.293.12909

    authors: Lajas AI,Pozo MJ,Salido GM,Singh J,Pariente JA

    更新日期:1996-01-01 00:00:00

  • Ezetimibe attenuates experimental diabetes and renal pathologies via targeting the advanced glycation, oxidative stress and AGE-RAGE signalling in rats.

    abstract::The current in-vivo study was premeditated to uncover the protective role of ezetimibe (EZ) against advanced glycation endproducts (AGEs)-related pathologies in experimental diabetes. Our results showed that EZ markedly improved the altered biochemical markers of diabetes mellitus (DM) (FBG, HbA1c, insulin, microalbum...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2021.1874996

    authors: Nabi R,Alvi SS,Shah A,Chaturvedi CP,Faisal M,Alatar AA,Ahmad S,Khan MS

    更新日期:2021-01-29 00:00:00

  • Pharmacological characterization of alpha1- and beta-adrenergic synergism of 5'DII activity in rat brown adipocytes.

    abstract::The role of adrenoceptor subtypes was studied in rat brown adipose tissue (BAT). The type II 5'-deiodinase (5'DII) was activated in response to simultaneous stimulation by beta3- and alpha1-adrenergic agonists, BRL 37344 or CGP 12177, and cirazoline, in brown adipocytes. Inhibition of the alpha1- and beta-adrenergic p...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813450500500464

    authors: Raasmaja A,York DA

    更新日期:2006-02-01 00:00:00

  • Comparison of potential preventive effects of pomegranate flower, peel and seed oil on insulin resistance and inflammation in high-fat and high-sucrose diet-induced obesity mice model.

    abstract:OBJECTIVE:The potentially beneficial effects of pomegranate peel (PPE), flower (PFE) and seed oil (PSO) extracts, in comparison with rosiglitazone, on adiposity, lipid profile, glucose homoeostasis, as well as on the underlying inflammatory mechanisms, were examined in high-fat and high-sucrose (HF/HS) diet-induced obe...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.3109/13813455.2016.1148053

    authors: Harzallah A,Hammami M,Kępczyńska MA,Hislop DC,Arch JR,Cawthorne MA,Zaibi MS

    更新日期:2016-01-01 00:00:00

  • Suppression of knee joint osteoarthritis induced secondary to type 2 diabetes mellitus in rats by resveratrol: role of glycated haemoglobin and hyperlipidaemia and biomarkers of inflammation and oxidative stress.

    abstract::Background: We investigated whether the anti-inflammatory and antioxidant agent, resveratrol can inhibit type 2 diabetes mellitus (T2DM)-induced osteoarthritis (OA) in rats and whether it is associated with the suppression of glycaemia, dyslipidemia and inflammatory and oxidative stress biomarkers.Materials and method...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1771378

    authors: Ebrahim HA,Alzamil NM,Al-Ani B,Haidara MA,Kamar SS,Dawood AF

    更新日期:2020-06-04 00:00:00

  • Fibrotic and apoptotic markers alteration in ovariectomised rats: addition of swimming training preserves lung architecture.

    abstract:CONTEXT:The important role of exercise in pulmonary function during menopause is not well known. Oestrogen deficiency in ageing female mice is accompanied by increase in apoptotic markers such as caspase3 in the lung. OBJECTIVE:The present study was designed to investigate whether swimming training will ameliorate fib...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2017.1396347

    authors: Daghigh F,Alihemmati A,Karimi P,Habibi P,Ahmadiasl N

    更新日期:2018-10-01 00:00:00

  • Effect of combined calcium, magnesium, vitamin C and E supplementation on seminal parameters and serum oxidative stress markers in fructose-induced diabetic Wistar rats.

    abstract::Context: Deleterious effects of diabetes on seminal quality, serum metals and antioxidants have been confirmed.Objective: This study evaluated the effect of combined calcium, magnesium, vitamin C and E supplementation on seminal parameters, serum total antioxidant capacity (TAC), nitric oxide (NO), malonyldialdehyde (...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1716017

    authors: Bassey IE,Ikpi DE,Isong IKP,Akpan UO,Onyeukwu CC,Nwankwo NP,Udofia IG

    更新日期:2020-01-25 00:00:00

  • Nutrition in the management of type 2 diabetes mellitus: review.

    abstract::Type 2 diabetes mellitus (T2DM) is the most prevalent disease and becoming a serious public health threat worldwide. In recent years, numerous effective T2DM intervention regimens have been developed, with promising results. However, these regimens are not usually economically available, and they are not well tolerate...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2019.1657899

    authors: El-Tantawy WH

    更新日期:2019-09-02 00:00:00

  • Use of a simple fractionation method to evaluate binding, internalization and intracellular distribution of oligonucleotides in vascular smooth muscle cells.

    abstract::Antisense oligonucleotides (ODN) are potent molecules that could be used to inhibit the synthesis of a protein specifically if delivered to the appropriate compartments (cytoplasm and nucleus) of the cell under study. We present here a simple method providing access to the fractions of internalized ODN available in th...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1076/apab.111.3.265.23465

    authors: Eboue D,Auger R,Angiari C,Le Doan T,Tenu JP

    更新日期:2003-07-01 00:00:00

  • Electrical activity recorded from abdominal surface before and after gastric surgery in man.

    abstract::To explain the role of the different portions of the stomach in the genesis of electrical signal obtained from abdominal surface (Electrogastrography, EGG), the EGG was recorded in 6 patients with gastric cancer and 7 with duodenal ulcer before and after total gastrectomy or Billroth II gastric resection, respectively...

    journal_title:Archives of physiology and biochemistry

    pub_type: 临床试验,杂志文章

    doi:10.1076/apab.104.1.50.12867

    authors: Riezzo G,Pezzolla F,Giorgio I

    更新日期:1996-01-01 00:00:00

  • Biomechanical events in the time to exhaustion at maximum aerobic speed.

    abstract::Recent studies reported good intra-individual reproducibility, but great inter-individual variation in a sample of elite athletes, in time to exhaustion (tlim) at the maximal aerobic speed (MAS: the lowest speed that elicits VO2max in an incremental treadmill test). The purpose of the present study was, on the one han...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1076/apab.105.6.583.3272

    authors: Gazeau F,Koralsztein JP,Billat V

    更新日期:1997-10-01 00:00:00

  • The modulatory effects of irisin on asprosin, leptin, glucose levels and lipid profile in healthy and obese male and female rats.

    abstract::Objectives: The main aim of this study was to investigate the effects of irisin on asprosin, leptin, glucose levels and lipid profile in healthy and obese male and female rats.Methods: Irisin was subcutaneously administered with osmotic minipumps at the dose of 100 ng/kg/day for 28 days and then, the serum levels of a...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1722706

    authors: Ozcan S,Ulker N,Bulmus O,Yardimci A,Ozcan M,Canpolat S

    更新日期:2020-02-06 00:00:00

  • Folic acid increases levels of GHS in brain of rats with oxidative stress induced with 3-nitropropionic acid.

    abstract::Aim: This study tested the hypothesis that folic acid (FA) modulates biogenic amines and protects the brain against oxidative stress induced by 3-nitropropionic acid (3NPA).Methods: Male Wistar rats received (groups of six) for 5 d: FA (50 mg/kg); 3NPA (10 mg/kg); or FA +3NPA. At last day, rats were sacrificed, and th...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2018.1484771

    authors: Calderón Guzmán D,Osnaya Brizuela N,Ortiz Herrera M,Juárez Olguín H,Valenzuela Peraza A,Hernández García E,Barragán Mejía G

    更新日期:2020-02-01 00:00:00

  • Comparison between the natural postnatal maturation and the spermine-induced maturation of the rat intestine.

    abstract::In the suckling rats, orally provided spermine induced structural and biochemical changes in the intestine, which are characteristics of the postnatal maturation. This induced maturation was compared to that occurring spontaneously. Eight mumol spermine were administered orally once a day, for one or three days, to su...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1076/apab.106.1.46.4392

    authors: Peulen O,Pirlet C,Klimek M,Goffinet G,Dandrifosse G

    更新日期:1998-02-01 00:00:00

  • Garlic (Allium sativum) improves anxiety- and depressive-related behaviors and brain oxidative stress in diabetic rats.

    abstract::This study investigated the effects of garlic on anxiety- and depression-related behaviors and brain oxidative markers in streptozotocin (STZ)-induced diabetes in rats. Fifty-six male Wistar rats were randomly divided into seven experimental groups (n = 8/group): control, diabetic + saline, diabetic + garlic, diabetic...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2018.1494746

    authors: Rahmani G,Farajdokht F,Mohaddes G,Babri S,Ebrahimi V,Ebrahimi H

    更新日期:2020-05-01 00:00:00

  • Association of hyperglycaemia and hyperlipidaemia with cognitive dysfunction in schizophrenia spectrum disorder.

    abstract::The objective of the study was to investigate the association of blood glucose and lipid profile parameters with cognitive impairment in schizophrenia. A total of 200 schizophrenia patients and 169 controls were enrolled in the study. Blood glucose and lipid profile were estimated in all the subjects. Cognition was as...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1839500

    authors: Nandeesha H,Keshri N,Rajappa M,Menon V

    更新日期:2020-11-03 00:00:00

  • Looking at proteins from two dimensions: a review on five decades of 2D electrophoresis.

    abstract::Separating proteins according to two different gel-electrophoretic methods not only increases the resolution power for highly complex samples when compared to one-dimensional separations, but is also a valuable tool for protein and protein complex characterization. There are a number of different electrophoresis metho...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章,评审

    doi:10.3109/13813455.2014.945188

    authors: Westermeier R

    更新日期:2014-12-01 00:00:00

  • Differential effect of high-fat, high-sucrose and combined high-fat/high-sucrose diets consumption on fat accumulation, serum leptin and cardiac hypertrophy in rats.

    abstract::The consumption of high calorie-content diets is the first cause of obesity, probably the main health issue worldwide; however, the experimental evidences for evaluating the differential metabolic modifications of high-sucrose or high-fat diets are scare. We evaluated the metabolic outcomes of the obesity induced by t...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2018.1517181

    authors: Gómez-Crisóstomo NP,De la Cruz-Hernández EN,Méndez Méndez ER,Hernández-Landero MF,Camacho Liévano JU,Martínez-Abundis E

    更新日期:2020-07-01 00:00:00

  • Exosomal expression of RAB27A and its related lncRNA Lnc-RNA-RP11-510M2 in lung cancer.

    abstract:OBJECTIVES:Examine the diagnostic role of serum exosomal RAB27A mRNA in lung cancer and evaluate the relation of LncRNAs to lung cancer in association to RAB27A mRNA in Egyptian population. METHODS:Exosomal RNA-based biomarkers RAB27A mRNA and Lnc-RNA-RP11-510M2.10 were selected based on bioinformatic methods, followe...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1778036

    authors: Mohamed Gamal El-Din G,Ibrahim FK,Shehata HH,Osman NM,Abdel-Rahman OM,Ali M

    更新日期:2020-07-13 00:00:00

  • Role of some circulating MiRNAs on breast cancer diagnosis.

    abstract::Aberrant expression of miRNAs has a link with tumorgenesis and their deregulation is reported in biological fluids of cancer patients. Authors aimed to investigate the diagnostic role of miRNA-17-5p, miR-155 and miRNA-222 in serum samples from breast cancer patients (n = 80), benign breast patients (n = 40) and health...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2018.1482355

    authors: Swellam M,Zahran RFK,Abo El-Sadat Taha H,El-Khazragy N,Abdel-Malak C

    更新日期:2019-12-01 00:00:00

  • Inhibition of notch signalling and mesangial expansion by combined glucagon like peptide-1 agonist and crocin therapy in animal model of diabetic nephropathy.

    abstract::Diabetic nephropathy (DN) is one of the devastating complications in diabetes mellitus (DM). Glucagon-like peptide-1 (GLP-1) is one of the incretins secreted from L cells in the intestine. Crocin (a carotenoid component of saffron) has antioxidants properties. We investigated the renal effects of Exendin-4 as a GLP-1 ...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1846203

    authors: Amin SN,El-Gamal EM,Rashed LA,Kamar SS,Haroun MA

    更新日期:2020-12-07 00:00:00

  • Quantitative electroencephalographic changes due to middle cerebral artery occlusion by endothelin 1 in conscious rats.

    abstract::The powerful vasoconstrictor peptide endothelin-1 (ET1) has been shown to reduce local cerebral blood flow in brain areas supplied by the middle cerebral artery (MCA) to a pathologically low level upon intracerebral injection adjacent to the MCA. This reduction manifests itself as an ischemic infarct, that is fully de...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1076/apab.106.5.384.4362

    authors: Moyanova S,Kortenska L,Kirov R,Iliev I

    更新日期:1998-12-01 00:00:00

  • Myo-inositol oxygenese activity in second trimester of pregnancy: altered myoinositol catabolism in gestational diabetes mellitus.

    abstract::Objective: We aimed to determine myo-inositol oxygenase (MIOX) activity in gestational diabetes mellitus (GDM).Material and Methods: The insulin, HbA1c, and MIOX levels of 80 pregnant women were analysed after 75 g OGTT. Group I included patients with no risk factor for GDM, Group II: patients with high risk for GDM, ...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1739714

    authors: Kısa B,Sert UY,Celik HK,Candar T,Erol Koç EM,Taşcı Y,Çağlar GS

    更新日期:2020-03-19 00:00:00

  • The effects of interval training on oxygen pulse and performance in supra-threshold runs.

    abstract::The aim of this study was to examine (i) the effects of a severe interval training period on oxygen pulse kinetics (O2-p, the ratio between VO2 and heart rate), and (ii) to study the consequences of these effects on the variation of performance (time to exhaustion) during severe runs. Seven athletes were tested before...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1076/apab.111.3.202.23455

    authors: Laffite LP,Mille-Hamard L,Koralsztein JP,Billat VL

    更新日期:2003-07-01 00:00:00

  • Myricetin derivative-rich fraction from Syzygium malaccense prevents high-fat diet-induced obesity, glucose intolerance and oxidative stress in C57BL/6J mice.

    abstract:AIM:A high-fat diet (HFD) can lead to obesity and related metabolic disorders. This study evaluated the preventive efficacy of myricetin derivative-rich fraction (MD) from Syzygium malaccense leaf extract against HFD-induced obesity, hyperglycaemia, and oxidative stress in C57BL/6J mice. METHODS:HFD-fed mice were admi...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1080/13813455.2020.1808019

    authors: Nallappan D,Ong KC,Palanisamy UD,Chua KH,Kuppusamy UR

    更新日期:2020-08-19 00:00:00

  • Hyper-insulinaemia and cancer, meta-analyses of epidemiological studies.

    abstract:BACKGROUND:A substantial body of evidence links sex hormones, diet, excess body weight and physical activity to the risk of developing cancer at several sites common in affluent countries. The hypothesis that high circulating levels of insulin could be the underlying factor increasing cancer risk has been proposed. Epi...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章,meta分析

    doi:10.1080/13813450801954451

    authors: Pisani P

    更新日期:2008-02-01 00:00:00

  • Age-dependent detection of erythrocytes glucose-6-phosphate dehydrogenase and its correlation with oxidative stress.

    abstract:CONTEXT:Glucose-6-phosphate dehydrogenase (G6PD) is an important enzyme of hexose monophosphate shunt, involved in the biosynthesis of reduced nicotinamide adenine dinucleotide phosphate hydrogen (NADPH). OBJECTIVE:This study was designed to investigate age-dependent changes in human erythrocyte G6PD activity. The G6P...

    journal_title:Archives of physiology and biochemistry

    pub_type: 杂志文章

    doi:10.3109/13813455.2015.1136648

    authors: Maurya PK,Kumar P,Chandra P

    更新日期:2016-01-01 00:00:00