Abstract:
:When neurotransmitters bind to their specific receptors in the membrane of nerve and muscle cells they induce conformational transitions leading to the formation of open receptor-channels and desensitized receptor forms. A knowledge of the rate and equilibrium constants associated with these transitions is required to (i) relate the mechanism of the receptor-mediated reaction to the resulting changes in transmembrane voltage that trigger signal transmission between neurons, (ii) calculate changes in transmembrane voltage that result from the interaction of diverse excitatory and inhibitory receptors in the same cell, and (iii) understand the mechanism by which receptor function is affected by activators, inhibitors, including clinically important compounds, and diseases of the nervous system. The conformational transitions of interest occur in the millisecond and the sub-millisecond time region. Chemical kinetic techniques for studying reactions mediated by membrane-bound neurotransmitter receptors in cells or vesicles in this time domain were not available. Here we describe the development and use of a laser pulse photolysis technique suitable for chemical kinetic investigations of neurotransmitter receptors in the mu s and ms time region. The type of information that can be obtained is also discussed.
journal_name
Arch Physiol Biochemjournal_title
Archives of physiology and biochemistryauthors
Hess GPdoi
10.1076/apab.104.6.752.12911subject
Has Abstractpub_date
1996-10-01 00:00:00pages
752-61issue
6eissn
1381-3455issn
1744-4160journal_volume
104pub_type
杂志文章,评审abstract:OBJECTIVE:The aim was to determine adenosine deaminase (ADA) and alkaline phosphatase (ALP) activity in sputum for the diagnosis of pulmonary tuberculosis. METHODS:This study comprised of 64 subjects (33 pulmonary tuberculosis and 31 lung cancer). The ADA and ALP activity was measured in sputum and serum. RESULTS:The...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2011.645545
更新日期:2012-02-01 00:00:00
abstract::The G0/G1 switch gene 2 (G0S2) protein attenuated adipose triglyceride lipase (ATGL) activity and decreased lipolysis in rodent and human adipocytes. We hypothesized that G0S2 mRNA expression in human adipose tissue is influenced by depot, adipocyte size, body weight and caloric intake. Adipose tissue samples were obt...
journal_title:Archives of physiology and biochemistry
pub_type: 临床试验,杂志文章
doi:10.3109/13813455.2015.1122066
更新日期:2016-01-01 00:00:00
abstract::Background: The microRNAs have come up as crucial mediators of energy balance and metabolic control. CD36 is potential biomarker of obesity and metabolic syndrome. This study investigates the concentration of miR-146a and miR-21 and CD 36 in blood samples of obese and healthy young participants. We assessed the associ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1775655
更新日期:2020-06-13 00:00:00
abstract::This study was to investigate whether epidermal growth factor (EGF) may induce any long-term effect on pancreatic exocrine function in vivo as well to evaluate the chronic effects of EGF on pancreatic growth in rats. Rats were treated with EGF (10 micrograms/kg) for 5 or 7 days. EGF infused intravenously (2 micrograms...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.104.3.293.12909
更新日期:1996-01-01 00:00:00
abstract::The current in-vivo study was premeditated to uncover the protective role of ezetimibe (EZ) against advanced glycation endproducts (AGEs)-related pathologies in experimental diabetes. Our results showed that EZ markedly improved the altered biochemical markers of diabetes mellitus (DM) (FBG, HbA1c, insulin, microalbum...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2021.1874996
更新日期:2021-01-29 00:00:00
abstract::The role of adrenoceptor subtypes was studied in rat brown adipose tissue (BAT). The type II 5'-deiodinase (5'DII) was activated in response to simultaneous stimulation by beta3- and alpha1-adrenergic agonists, BRL 37344 or CGP 12177, and cirazoline, in brown adipocytes. Inhibition of the alpha1- and beta-adrenergic p...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813450500500464
更新日期:2006-02-01 00:00:00
abstract:OBJECTIVE:The potentially beneficial effects of pomegranate peel (PPE), flower (PFE) and seed oil (PSO) extracts, in comparison with rosiglitazone, on adiposity, lipid profile, glucose homoeostasis, as well as on the underlying inflammatory mechanisms, were examined in high-fat and high-sucrose (HF/HS) diet-induced obe...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2016.1148053
更新日期:2016-01-01 00:00:00
abstract::Background: We investigated whether the anti-inflammatory and antioxidant agent, resveratrol can inhibit type 2 diabetes mellitus (T2DM)-induced osteoarthritis (OA) in rats and whether it is associated with the suppression of glycaemia, dyslipidemia and inflammatory and oxidative stress biomarkers.Materials and method...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1771378
更新日期:2020-06-04 00:00:00
abstract:CONTEXT:The important role of exercise in pulmonary function during menopause is not well known. Oestrogen deficiency in ageing female mice is accompanied by increase in apoptotic markers such as caspase3 in the lung. OBJECTIVE:The present study was designed to investigate whether swimming training will ameliorate fib...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2017.1396347
更新日期:2018-10-01 00:00:00
abstract::Context: Deleterious effects of diabetes on seminal quality, serum metals and antioxidants have been confirmed.Objective: This study evaluated the effect of combined calcium, magnesium, vitamin C and E supplementation on seminal parameters, serum total antioxidant capacity (TAC), nitric oxide (NO), malonyldialdehyde (...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1716017
更新日期:2020-01-25 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is the most prevalent disease and becoming a serious public health threat worldwide. In recent years, numerous effective T2DM intervention regimens have been developed, with promising results. However, these regimens are not usually economically available, and they are not well tolerate...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2019.1657899
更新日期:2019-09-02 00:00:00
abstract::Antisense oligonucleotides (ODN) are potent molecules that could be used to inhibit the synthesis of a protein specifically if delivered to the appropriate compartments (cytoplasm and nucleus) of the cell under study. We present here a simple method providing access to the fractions of internalized ODN available in th...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.111.3.265.23465
更新日期:2003-07-01 00:00:00
abstract::To explain the role of the different portions of the stomach in the genesis of electrical signal obtained from abdominal surface (Electrogastrography, EGG), the EGG was recorded in 6 patients with gastric cancer and 7 with duodenal ulcer before and after total gastrectomy or Billroth II gastric resection, respectively...
journal_title:Archives of physiology and biochemistry
pub_type: 临床试验,杂志文章
doi:10.1076/apab.104.1.50.12867
更新日期:1996-01-01 00:00:00
abstract::Recent studies reported good intra-individual reproducibility, but great inter-individual variation in a sample of elite athletes, in time to exhaustion (tlim) at the maximal aerobic speed (MAS: the lowest speed that elicits VO2max in an incremental treadmill test). The purpose of the present study was, on the one han...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.105.6.583.3272
更新日期:1997-10-01 00:00:00
abstract::Objectives: The main aim of this study was to investigate the effects of irisin on asprosin, leptin, glucose levels and lipid profile in healthy and obese male and female rats.Methods: Irisin was subcutaneously administered with osmotic minipumps at the dose of 100 ng/kg/day for 28 days and then, the serum levels of a...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1722706
更新日期:2020-02-06 00:00:00
abstract::Aim: This study tested the hypothesis that folic acid (FA) modulates biogenic amines and protects the brain against oxidative stress induced by 3-nitropropionic acid (3NPA).Methods: Male Wistar rats received (groups of six) for 5 d: FA (50 mg/kg); 3NPA (10 mg/kg); or FA +3NPA. At last day, rats were sacrificed, and th...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1484771
更新日期:2020-02-01 00:00:00
abstract::In the suckling rats, orally provided spermine induced structural and biochemical changes in the intestine, which are characteristics of the postnatal maturation. This induced maturation was compared to that occurring spontaneously. Eight mumol spermine were administered orally once a day, for one or three days, to su...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.106.1.46.4392
更新日期:1998-02-01 00:00:00
abstract::This study investigated the effects of garlic on anxiety- and depression-related behaviors and brain oxidative markers in streptozotocin (STZ)-induced diabetes in rats. Fifty-six male Wistar rats were randomly divided into seven experimental groups (n = 8/group): control, diabetic + saline, diabetic + garlic, diabetic...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1494746
更新日期:2020-05-01 00:00:00
abstract::The objective of the study was to investigate the association of blood glucose and lipid profile parameters with cognitive impairment in schizophrenia. A total of 200 schizophrenia patients and 169 controls were enrolled in the study. Blood glucose and lipid profile were estimated in all the subjects. Cognition was as...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1839500
更新日期:2020-11-03 00:00:00
abstract::Separating proteins according to two different gel-electrophoretic methods not only increases the resolution power for highly complex samples when compared to one-dimensional separations, but is also a valuable tool for protein and protein complex characterization. There are a number of different electrophoresis metho...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,评审
doi:10.3109/13813455.2014.945188
更新日期:2014-12-01 00:00:00
abstract::The consumption of high calorie-content diets is the first cause of obesity, probably the main health issue worldwide; however, the experimental evidences for evaluating the differential metabolic modifications of high-sucrose or high-fat diets are scare. We evaluated the metabolic outcomes of the obesity induced by t...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1517181
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVES:Examine the diagnostic role of serum exosomal RAB27A mRNA in lung cancer and evaluate the relation of LncRNAs to lung cancer in association to RAB27A mRNA in Egyptian population. METHODS:Exosomal RNA-based biomarkers RAB27A mRNA and Lnc-RNA-RP11-510M2.10 were selected based on bioinformatic methods, followe...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1778036
更新日期:2020-07-13 00:00:00
abstract::Aberrant expression of miRNAs has a link with tumorgenesis and their deregulation is reported in biological fluids of cancer patients. Authors aimed to investigate the diagnostic role of miRNA-17-5p, miR-155 and miRNA-222 in serum samples from breast cancer patients (n = 80), benign breast patients (n = 40) and health...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2018.1482355
更新日期:2019-12-01 00:00:00
abstract::Diabetic nephropathy (DN) is one of the devastating complications in diabetes mellitus (DM). Glucagon-like peptide-1 (GLP-1) is one of the incretins secreted from L cells in the intestine. Crocin (a carotenoid component of saffron) has antioxidants properties. We investigated the renal effects of Exendin-4 as a GLP-1 ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1846203
更新日期:2020-12-07 00:00:00
abstract::The powerful vasoconstrictor peptide endothelin-1 (ET1) has been shown to reduce local cerebral blood flow in brain areas supplied by the middle cerebral artery (MCA) to a pathologically low level upon intracerebral injection adjacent to the MCA. This reduction manifests itself as an ischemic infarct, that is fully de...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.106.5.384.4362
更新日期:1998-12-01 00:00:00
abstract::Objective: We aimed to determine myo-inositol oxygenase (MIOX) activity in gestational diabetes mellitus (GDM).Material and Methods: The insulin, HbA1c, and MIOX levels of 80 pregnant women were analysed after 75 g OGTT. Group I included patients with no risk factor for GDM, Group II: patients with high risk for GDM, ...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1739714
更新日期:2020-03-19 00:00:00
abstract::The aim of this study was to examine (i) the effects of a severe interval training period on oxygen pulse kinetics (O2-p, the ratio between VO2 and heart rate), and (ii) to study the consequences of these effects on the variation of performance (time to exhaustion) during severe runs. Seven athletes were tested before...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1076/apab.111.3.202.23455
更新日期:2003-07-01 00:00:00
abstract:AIM:A high-fat diet (HFD) can lead to obesity and related metabolic disorders. This study evaluated the preventive efficacy of myricetin derivative-rich fraction (MD) from Syzygium malaccense leaf extract against HFD-induced obesity, hyperglycaemia, and oxidative stress in C57BL/6J mice. METHODS:HFD-fed mice were admi...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.1080/13813455.2020.1808019
更新日期:2020-08-19 00:00:00
abstract:BACKGROUND:A substantial body of evidence links sex hormones, diet, excess body weight and physical activity to the risk of developing cancer at several sites common in affluent countries. The hypothesis that high circulating levels of insulin could be the underlying factor increasing cancer risk has been proposed. Epi...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章,meta分析
doi:10.1080/13813450801954451
更新日期:2008-02-01 00:00:00
abstract:CONTEXT:Glucose-6-phosphate dehydrogenase (G6PD) is an important enzyme of hexose monophosphate shunt, involved in the biosynthesis of reduced nicotinamide adenine dinucleotide phosphate hydrogen (NADPH). OBJECTIVE:This study was designed to investigate age-dependent changes in human erythrocyte G6PD activity. The G6P...
journal_title:Archives of physiology and biochemistry
pub_type: 杂志文章
doi:10.3109/13813455.2015.1136648
更新日期:2016-01-01 00:00:00