Abstract：
:The effects of two DNA aptamers (oligonucleotides) 15TBA and 31TBA (15- and 31-mer thrombin-binding aptamers, respectively) on thrombin activity were studied. Both aptamers added to human plasma dose-dependently increased thrombin time (fibrin formation upon exposure to exogenous thrombin), prothrombin time (clotting activation by the extrinsic pathway), and activated partial thromboplastin time (clotting activation by the intrinsic pathway). At the same time, these aptamers did not modify amidolytic activity of thrombin evaluated by cleavage of synthetic chromogenic substrate. Aptamers also inhibited thrombin-induced human platelet aggregation. The inhibitory effects of 31TBA manifested at lower concentrations than those of 15TBA in all tests. These data indicate that the studied antithrombin DNA aptamers effectively suppress its two key reactions, fibrin formation and stimulation of platelet aggregation, without modifying active center of the thrombin molecule.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Dobrovolsky AB,Titaeva EV,Khaspekova SG,Spiridonova VA,Kopylov AM,Mazurov AVdoi
10.1007/s10517-009-0627-7subject
Has Abstractpub_date
2009-07-01 00:00:00pages
33-6issue
1eissn
0007-4888issn
1573-8221journal_volume
148pub_type
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