Abstract：
:In a previous study, we demonstrated that cholera toxin-A subunit, as well as the whole toxin, selectively blocks opioid-induced prolongation of the Ca2+ component of the action potential duration (APD) in dorsal root ganglion (DRG) neurons, indicating mediation of this excitatory effect by Gs-linked opioid receptors. The present study shows that pretreatment of DRG neurons with the B subunit of cholera toxin (1-10 ng/ml; greater than 15 min) can also block mu/delta and kappa opioid-induced APD prolongation, but not shortening. Since the B subunit binds selectively to GM1 ganglioside located on the cell surface, these results suggest that this ganglioside may regulate Gs-linked excitatory opioid receptor functions in DRG neurons. Possible contamination of purified B subunit preparations of cholera toxin with traces of the more potent A subunit was eliminated by heating the stock solution to 56 degrees C for 20 min. Exposure of DRG neurons to an affinity-purified anti-GM1 antiserum also blocked opioid-induced APD prolongation, providing further evidence that GM1 ganglioside may play an essential role in excitatory opioid modulation of the action potential of these cells. The blockade by cholera toxin-B subunit and anti-GM1 antibodies of opioid-induced APD prolongation is best accounted for by the following hypothesis: CTX-B interferes with an endogenous GM1 ganglioside component of the excitatory, but not inhibitory, opioid receptor complex on DRG neurons that may allosterically regulate coupling of the receptors via Gs to adenylate cyclase/cyclic adenosine monophosphate-dependent ionic conductances.
journal_name
Brain Resjournal_title
Brain researchauthors
Shen KF,Crain SMdoi
10.1016/0006-8993(90)90751-vsubject
Has Abstractpub_date
1990-10-29 00:00:00pages
1-7issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(90)90751-Vjournal_volume
531pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The patch-clamp technique was used to characterize the single channel ion currents in primary cultures of rat astrocytes. The most dominant channel type, which was found in over half of the inside-out membrane patches, was a potassium channel. The measured reversal potential was -67 mV, which is close to the calculate...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90360-0
更新日期：1989-04-10 00:00:00
abstract：:The content of serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and norepinephrine (NE) was analysed in 71 human spinal cords obtained post-mortem. The highest content of 5-HT, 5-HIAA and NE was found in the lumbar enlargement of the spinal cord. 5-HT and 5-HIAA content increased from fetal to adult spinal cord w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91613-7
更新日期：1988-03-08 00:00:00
abstract：:The hallucinogen d-lysergic acid diethylamide (d-LSD) evokes dramatic somatic and psychological effects. In order to analyze the neural activation induced by this unique psychoactive drug, we tested the hypothesis that expression of the immediate-early gene product c-Fos is induced in specific regions of the rat foreb...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03548-5
更新日期：2002-12-27 00:00:00
abstract：:Because brainstem auditory evoked potentials (BAEPs) are frequently recorded in anesthetized animals and humans, it is important to become familiar with the effects on the BAEP of drugs used during anesthesia, including pre-anesthetics. The dose-dependent and stimulus intensity-dependent effects on the BAEP of a pre-a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90221-1
更新日期：1988-07-26 00:00:00
abstract：:The quantitative autoradiographic method with L-(35S)methionine was used to determine the effects of 1-acute (4h) and 2-chronic (14 days) immobilization stress followed by one week of recovery. Acute stress induced a significant decrease in methionine incorporation into proteins in 17 of the 35 brain structures examin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00046-s
更新日期：1995-03-27 00:00:00
abstract：:The purpose of this study was to examine whether endothelium-mediated dilation is responsible for the cortical hyperemia that occurs during cortical spreading depression (CSD) in rats using three different approaches. The first approach taken was the acute pharmacological inhibition of the predominant endothelium-cent...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)03352-2
更新日期：2002-02-22 00:00:00
abstract：:Beta-amyloid (Abeta) peptides, the major component of senile plaques in brains of patients with Alzheimer's disease (AD), were found in the low pH organelles (i.e. endosome/lysosome) of cultured neuronal cells. Since acidic pH values have been shown to promote the self-assembly of Abetas, which seems to be a prerequis...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03322-9
更新日期：2001-03-02 00:00:00
abstract：:The content and turnover of dopamine, norepinephrine and 5-hydroxytryptamine (serotonin), and the content of their respective major metabolites were evaluated in 19 discrete brain areas of rats exposed to conditioned emotional response (CER), and in control groups which received either equivalent yoked shock (shock on...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90647-3
更新日期：1982-05-20 00:00:00
abstract：:In anaesthetized cats, effects of iontophoretic application of angiotensin II were studied in the nuclei of the tractus solitarius. Specifically, its effects were tested on single respiratory neurones, having rhythmic activity related temporally with the phrenic nerve discharge, and on presumed reflex interneurones, w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91232-7
更新日期：1987-03-24 00:00:00
abstract：:The lamination of the hippocampus in the homozygous B6CBA weaver mouse (wv/wv) was compared with that in normal B6CBA littermates (+/+) and C57BL/6J mice using Nissl and Timm's staining. In Nissl-stained preparations, the normal littermates exhibit a compact, regular arrangement of pyramidal cells in area CA3 of the h...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00974-u
更新日期：1995-10-23 00:00:00
abstract：:We examined the in vivo synthesis of brain phosphatidylcholine (PC) by the methylation of phosphatidylethanolamine (PE). [3H-methyl]methionine was infused i.c.v., by indwelling cannula, and brain samples were taken 0.5-18 h thereafter and assayed for [3H]PC, as well as for its biosynthetic intermediates [3H]phosphatid...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90576-2
更新日期：1987-09-01 00:00:00
abstract：:Tuberohypophysial dopamine (DA) neurons terminate in the intermediate and neural lobes of the posterior pituitary. The objective of this study was to determine if concentrations of 3,4-dihydroxyphenylacetic acid (DOPAC), a major metabolite of DA in these regions, reflect the activity of tuberohypophysial DA neurons. T...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91209-y
更新日期：1990-01-01 00:00:00
abstract：:Intracellular recordings in vivo from serotonergic dorsal raphe neurons of the rat brain reveal that these cells undergo a pronounced postspike hyperpolarization followed by a gradual interspike depolarization leading to the succeeding spike. Such repetitive cycles of interspike hyperpolarization and depolarization, w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90124-x
更新日期：1982-04-29 00:00:00
abstract：:In the present study, we used a repeated restraint stress animal model to observe the changes in the expression of brain-derived neurotrophic factor (BDNF) and B cell lymphoma protein-2 (Bcl-2) in hippocampal neurons of rats, monitored the time course of the expression over 3 weeks post-stress period, and examined the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.06.089
更新日期：2004-10-29 00:00:00
abstract：:The cellular mechanism for the suppression of GnRH gene expression by the phorbol ester PMA was investigated in GT1 cells. The protein synthesis inhibitor cycloheximide decreased GnRH primary transcript levels, indicating a protein synthesis requirement for basal GnRH transcription. PMA decreased GnRH primary transcri...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01479-5
更新日期：1997-03-28 00:00:00
abstract：:We examined the in vitro effects of neurotransmitters, high KCl as well as sialic acid-containing compounds (GM1; SC) on transglutaminase (TG) activity in isolated superior cervical ganglia (SCG) one week after denervation or axotomy. Following denervation, TG activity in SCG decreased to 83% of the unoperated control...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90352-n
更新日期：1993-02-26 00:00:00
abstract：:In a previous study, we have demonstrated that an ovalbumin-like antigen is present within approximately one-half of all neurons of chicken spinal ganglia. The current study demonstrates this antigen co-localizes absolutely with neural intermediate filament protein (Peripherin) in small to medium-sized neurons of spin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90342-k
更新日期：1993-11-12 00:00:00
abstract：:Seasonally breeding animals undergo numerous physiological changes in response to changes in the length of the photoperiod. In most warm-weather breeding rodents, these changes result in reproductive quiescence during short photoperiods. It has been hypothesized that this change is mediated by changes in the activity ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.08.083
更新日期：2007-11-14 00:00:00
abstract：:GABA-A receptors mediate both phasic synaptic inhibition and more recently appreciated tonic currents in the vertebrate central nervous system. We addressed discrepancies in the literature regarding the pharmacology of tonic currents by examining tonic currents in a controlled environment of dissociated, solitary glut...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.08.033
更新日期：2006-11-06 00:00:00
abstract：:Dorsal root ganglia (DRG) are key elements in sensory signaling under physiological and pathological conditions. Little is known about electrical coupling among cells in these ganglia. In this study, we injected the fluorescent dye Lucifer yellow (LY) into single cells to examine dye coupling in DRG. We found no dye c...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.12.021
更新日期：2005-03-02 00:00:00
abstract：:In the following study the behavioural effects of simultaneous lesion of the nucleus basalis magnocellularis (NBM) using ibotenic acid and noradrenergic depletion following a single i.p. administration of DSP4 (50 mg/kg) were examined in the rat. NBM lesion induced a deficit in acquisition of a reinforced T-maze alter...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91067-o
更新日期：1992-11-13 00:00:00
abstract：:It has been confirmed that gabapentin (GBP) induced a inhibition of the voltage-gated persistent sodium current in chronically compressed dorsal root ganglion (DRG) neurons. The persistent sodium current is found in excitable DRG neurons of painful diabetic neuropathy (PDN) rats where it is mediated by tetrodotoxin (T...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.11.032
更新日期：2013-02-01 00:00:00
abstract：:In this study, the effects of early vs. delayed tPA treatment on the development of hemorrhagic transformation was compared in a rat thromboembolic model of stroke. Fibrinolysis was performed by administering tPA intravenously at 2 or 6 h after ischemic onset. Twenty-four hours later, confluent hemorrhagic infarction ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)02276-3
更新日期：2000-01-31 00:00:00
abstract：:The present study was designed to determine the types of acetylcholine receptors involved in the initiation of epileptic seizures from the zona incerta and surrounding structures by cholinergic stimulation in rats. Unilateral intracerebral microinjection of the mixed muscarinic and nicotinic agonist carbachol (3 micro...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90017-5
更新日期：1994-04-18 00:00:00
abstract：:The opposing actions of diphenylhydantoin (DPH) and calcium on the level of [32P]phosphate incorporation into particular rat and human brain proteins have been demonstrated in this study by employing the techniques of acrylamide gel electrophoresis and autoradiography. In the presence of calcium several brain proteins...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(77)90930-1
更新日期：1977-09-23 00:00:00
abstract：:Neurofilament expression in peripheral olfactory neurons of adult rats was investigated by immunoblotting and immunohistochemistry using monoclonal antibodies specific for each of the 3 neurofilament proteins. Immunoblotting analysis of olfactory epithelium extracts demonstrated the presence of only the 200 kDa (NFH) ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90416-s
更新日期：1991-05-31 00:00:00
abstract：:The rubrointerposital projection was studied in cats where wheat germ agglutinin-horseradish peroxidase (WGA-HRP) was implanted in various parts of the interposed nuclei. No retrogradely labelled rubral cells were observed following implantations in the posterior interposed nucleus, and only very few such cells were i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91370-3
更新日期：1986-11-05 00:00:00
abstract：:Changes in the expression and activation of protein kinase C (PKC) have been implicated in the pathogenesis of diabetic neuropathy. Recent studies in liver, retina, and cardiovascular tissues from experimentally diabetic rats have demonstrated that diabetes has a selective effect on the expression and subcellular dist...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00062-0
更新日期：1997-04-18 00:00:00
abstract：:Physical exercise is likely to alter brain function and to afford neuroprotection in several neurological diseases. Although the favorable effects of physical exercise on traumatic brain injury (TBI) patients is well known, little information is available regarding the role of free radicals in the improvement induced ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.04.052
更新日期：2009-07-07 00:00:00
abstract：OBJECTIVE:The modern brain imaging studies in patients with eating disorders have shown that neurotransmitting regulation differs distinctly from control groups. These disturbances have involved serotonin and dopamine system can be inherited and conditioned by genes. The aim of this work has been the analysis of associ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.10.035
更新日期：2010-01-11 00:00:00
