The complexity of the secreted NPA and FAR lipid-binding protein families of Haemonchus contortus revealed by an iterative proteomics-bioinformatics approach.

abstract：:A Plasmodium falciparum gene closely linked to the chloroquine resistance locus encodes PfCG2, a predicted 320-330kDa protein. In the parasitized erythrocyte, PfCG2 expression rises sharply in the trophozoite stage and is detected in electron-dense patches along the parasitophorous vacuolar membrane (PVM), in the cyto...

journal_title：Molecular and biochemical parasitology

authors： Cooper RA,Papakrivos J,Lane KD,Fujioka H,Lingelbach K,Wellems TE

更新日期：2005-12-01 00:00:00

abstract：:Giardia lamblia is an early diverging eukaryote which causes gastrointestinal disease throughout the world. Different subgroups of Giardia have been defined based on several biochemical and genetic criteria. We have developed a method for stably introducing DNA into the nuclei of the parasite using puromycin acetyltra...

journal_title：Molecular and biochemical parasitology

authors： Singer SM,Yee J,Nash TE

更新日期：1998-04-01 00:00:00

abstract：:Intraerythrocytic Plasmodium falciparum digests vast amounts of hemoglobin within an acidic food vacuole (FV). Four homologous aspartic proteases participate in hemoglobin degradation within the FV. Plasmepsin (PM) I and II are thought to initiate degradation of the native hemoglobin molecule. PM IV and histo-aspartic...

journal_title：Molecular and biochemical parasitology

authors： Banerjee R,Francis SE,Goldberg DE

更新日期：2003-07-01 00:00:00

abstract：:Procyclic Trypanosoma brucei brucei strain 366D is susceptible to DL-alpha-difluoromethylornithine (DFMO) with an in vitro ED50 value of 225 microM. A mutant of the procyclic strain resistant to 20 mM of DFMO was isolated by serial in vitro passages of the organisms in increasing concentrations of the drug. Drug resis...

journal_title：Molecular and biochemical parasitology

authors： Phillips MA,Wang CC

更新日期：1987-01-02 00:00:00

abstract：:Mitochondrial ATPase of Leishmania donovani was characterized using digitonin-permeabilized promastigotes and the results were compared with those from isolated mitochondria. Maximum mitochondrial ATPase activity was obtained in promastigotes permeabilized with digitonin at a final concentration of 20 microM and the s...

journal_title：Molecular and biochemical parasitology

authors： Das A

更新日期：1993-08-01 00:00:00

abstract：:Human infections from Plasmodium knowlesi present challenges to malaria control in Southeast Asia. P. knowlesi also offers a model for other human malaria species including Plasmodium vivax. P. knowlesi parasites can be cultivated in the laboratory, and their transformation is standardly performed by direct electropor...

journal_title：Molecular and biochemical parasitology

authors： Moraes Barros RR,Gibson TJ,Kite WA,Sá JM,Wellems TE

更新日期：2017-12-01 00:00:00

abstract：:The schizont stage of the protozoan parasite Theileria annulata reversibly transforms bovine monocytes into an immortalised and metastatic state. We have been studying T. annulata induction of host matrix metalloproteinases (MMP) which are involved in parasite dissemination and pathogenesis. We have observed that prol...

journal_title：Molecular and biochemical parasitology

authors： Adamson R,Logan M,Kinnaird J,Langsley G,Hall R

更新日期：2000-02-25 00:00:00

abstract：:Three days after mice were inoculated with Trypanosoma brucei gambiense, total hepatic cytochrome P-450 levels were decreased 14% from control values, while the specific mixed-function oxidase activity (per nmole of cytochrome P-450) was inhibited almost 40% in infected animals. Furthermore, drugs used to treat trypan...

journal_title：Molecular and biochemical parasitology

authors： Shertzer HG,Hall JE,Seed JR

更新日期：1981-08-01 00:00:00

abstract：:The pyrimidine biosynthesis pathway in the protozoan pathogen Toxoplasma gondii is essential for parasite growth during infection. To investigate the properties of dihydroorotate dehydrogenase (TgDHOD), the fourth enzyme in the T. gondii pyrimidine pathway, we expressed and purified recombinant TgDHOD. TgDHOD exhibite...

journal_title：Molecular and biochemical parasitology

authors： Hortua Triana MA,Huynh MH,Garavito MF,Fox BA,Bzik DJ,Carruthers VB,Löffler M,Zimmermann BH

更新日期：2012-08-01 00:00:00

abstract：:To investigate whether the lysosomes of Trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used Triton WR-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. In...

journal_title：Molecular and biochemical parasitology

authors： Opperdoes FR,Van Roy J

更新日期：1982-09-01 00:00:00

abstract：:The glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) of Trypanosoma brucei is the most abundant GPI-anchored protein expressed on any cell, and is an essential virulence factor. To determine what structural features affect efficient expression of VSG, we made a series of mutations in two ...

journal_title：Molecular and biochemical parasitology

authors： Wang J,Böhme U,Cross GA

更新日期：2003-05-01 00:00:00

abstract：:The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

journal_title：Molecular and biochemical parasitology

authors： Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

更新日期：2010-10-01 00:00:00

abstract：:Root-knot nematodes (Meloidogyne spp.) are among the most important plant pathogens. In this study, the mitochondrial (mt) genomes of the root-knot nematodes, M. chitwoodi and M. incognita were sequenced. PCR analyses suggest that both mt genomes are circular, with an estimated size of 19.7 and 18.6-19.1kb, respective...

journal_title：Molecular and biochemical parasitology

authors： Humphreys-Pereira DA,Elling AA

更新日期：2014-03-01 00:00:00

abstract：:After a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. The data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. Trypanosomes take up o...

journal_title：Molecular and biochemical parasitology

authors： Fairlamb AH,Bowman IB

更新日期：1980-10-01 00:00:00

abstract：:GRA3 is a 30-kDa protein located inside the dense granules of Toxoplasma gondii. Following invasion and exocytosis of dense granules within the parasitophorous vacuole, GRA3 becomes associated with the parasitophorous vacuolar membrane (PVM) and extensions of the PVM which protrude into the cytoplasm. A partial cDNA e...

journal_title：Molecular and biochemical parasitology

authors： Bermudes D,Dubremetz JF,Achbarou A,Joiner KA

更新日期：1994-12-01 00:00:00

abstract：:Heartworm disease caused by Dirofilaria immitis affects canine and feline hosts. Moreover, the parasite can infect humans, causing pulmonary dirofilariosis. Most affected dogs have patent infections with circulating microfilariae in peripheral blood, although infected dogs sometimes develop occult infections character...

journal_title：Molecular and biochemical parasitology

authors： Oleaga A,Pérez-Sánchez R,Pagés E,Marcos-Atxutegi C,Simón F

更新日期：2009-08-01 00:00:00

abstract：:The use of transfection in the study of the biology of malaria parasites has been limited due to poor transfection efficiencies (frequency of 10(-6) to 10(-9)) and a paucity of selection markers. Here, a new method of transfection, using non-viral Nucleofector technology, is described for the rodent parasite Plasmodiu...

journal_title：Molecular and biochemical parasitology

authors： Janse CJ,Franke-Fayard B,Mair GR,Ramesar J,Thiel C,Engelmann S,Matuschewski K,van Gemert GJ,Sauerwein RW,Waters AP

更新日期：2006-01-01 00:00:00

abstract：:The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated b...

journal_title：Molecular and biochemical parasitology

authors： Perry DA,Morrison HG,Adam RD

更新日期：2011-12-01 00:00:00

abstract：:Replication of kinetoplast DNA minicircles in Crithidia fasciculata occurs by a unidirectional mechanism involving continuous synthesis of one strand (L strand) and discontinuous synthesis of the complementary strand (H strand). L-strands are initiated by RNA priming at alternate origins (A and B) resulting in daughte...

journal_title：Molecular and biochemical parasitology

authors： Hines JC,Engel ML,Zhao H,Ray DS

更新日期：2001-06-01 00:00:00

abstract：:We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(...

journal_title：Molecular and biochemical parasitology

authors： Gilchrist CA,Streets HL,Ackers JP,Hall R

更新日期：1995-10-01 00:00:00

abstract：:Post-transcriptional regulation is a key feature controlling gene expression in the protozoan parasite Leishmania. The nine-nucleotide paraflagellar rod regulatory element (PRE) in the 3'UTR of Leishmania mexicana PFR2 is both necessary and sufficient for the observed 10-fold higher level of PFR2 mRNA in promastigotes...

journal_title：Molecular and biochemical parasitology

authors： Holzer TR,Mishra KK,LeBowitz JH,Forney JD

更新日期：2008-01-01 00:00:00

abstract：:A gene-family, named sep, encoding small exported proteins conserved across Plasmodium species has been identified. SEP proteins (13-16 kDa) contain a predicted signal peptide at the NH(2)-terminus, an internal hydrophobic region and a polymorphic, low-complexity region at the carboxy-terminus. One member of the Plasm...

journal_title：Molecular and biochemical parasitology

authors： Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

更新日期：2003-02-01 00:00:00

abstract：:Helminths are masterful immunoregulators. A characteristic feature of helminth infection is a Th2-dominated immune response, but stimulation of immunoregulatory cell populations, such as regulatory T cells and alternatively activated macrophages, is equally common. Typically, Th1/17 immunity is blocked and productive ...

journal_title：Molecular and biochemical parasitology

authors： Hewitson JP,Grainger JR,Maizels RM

更新日期：2009-09-01 00:00:00

abstract：:We have previously provided evidence for a pathway in Plasmodium falciparum-infected erythrocytes, coined the parasitophorous duct pathway, which provides serum (macro)molecules direct access to intraerythrocytic parasites . The present study addresses the purity of the fluorescent macromolecules used to define the du...

journal_title：Molecular and biochemical parasitology

authors： Goodyer ID,Pouvelle B,Schneider TG,Trelka DP,Taraschi TF

更新日期：1997-07-01 00:00:00

abstract：:Giardia lamblia undergoes surface antigenic variation. The variant-specific surface proteins (VSPs) of isolate WB are cysteine-rich, can vary dramatically in size, contain Cys-X-X-Cys motifs, and are differentially expressed. GS/M(H7) is a Giardia clone from a different isolate which expresses a VSP epitope not found ...

journal_title：Molecular and biochemical parasitology

authors： Nash TE,Mowatt MR

更新日期：1992-04-01 00:00:00

abstract：:Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

journal_title：Molecular and biochemical parasitology

authors： Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

更新日期：2012-03-01 00:00:00

abstract：:The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

journal_title：Molecular and biochemical parasitology

authors： Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

更新日期：1990-12-01 00:00:00

abstract：:The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

journal_title：Molecular and biochemical parasitology

authors： Cameron S,Fyffe SA,Goldie S,Hunter WN

更新日期：2008-04-01 00:00:00

abstract：:Intra-erythrocytic Plasmodium parasites digest host cell haemoglobin and use the liberated amino acids for protein synthesis. Although several endoproteases (aspartic, cysteine, and metallo-) have been shown to be involved in the initial stages of haemoglobin degradation, little is known about the steps immediately be...

journal_title：Molecular and biochemical parasitology

authors： Gavigan CS,Dalton JP,Bell A

更新日期：2001-09-28 00:00:00

abstract：:A hydrophobic, galactose-specific lectin was isolated by means of affinity chromatography from Sarcocystis gigantea. Adsorbents with different spacer lengths were tested. S. gigantea lectin differs from sheep muscle lectin in the spacer length needed for adsorption. Sodium dodecyl sulfate-gel electrophoresis of the S....

journal_title：Molecular and biochemical parasitology

authors： Mácha J,Procházková Z,Cerva L,Gut J

更新日期：1985-09-01 00:00:00