Food vacuole plasmepsins are processed at a conserved site by an acidic convertase activity in Plasmodium falciparum.


:Intraerythrocytic Plasmodium falciparum digests vast amounts of hemoglobin within an acidic food vacuole (FV). Four homologous aspartic proteases participate in hemoglobin degradation within the FV. Plasmepsin (PM) I and II are thought to initiate degradation of the native hemoglobin molecule. PM IV and histo-aspartic protease (HAP) act on denatured globin further downstream in the pathway. PM I and II have been shown to be synthesized as zymogens and activated by proteolytic removal of a propiece. In this study, we have determined that the proteolytic processing of FV plasmepsins occurs immediately after a conserved Leu-Gly dipeptidyl motif with uniform kinetics and pH and inhibitor sensitivities. We have developed a cell-free in vitro processing assay that generates correctly processed plasmepsins. Our data suggest that proplasmepsin processing is not autocatalytic, but rather is mediated by a separate processing enzyme. This convertase requires acidic conditions and is blocked only by the calpain inhibitors, suggesting that it may be an atypical calpain-like protease.


Mol Biochem Parasitol


Banerjee R,Francis SE,Goldberg DE




Has Abstract


2003-07-01 00:00:00














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    authors: Knapp B,Hundt E,Nau U,Küpper HA

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    authors: Marín-Menéndez A,Monaghan P,Bell A

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    abstract::The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    authors: Cooper RA,Papakrivos J,Lane KD,Fujioka H,Lingelbach K,Wellems TE

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


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    更新日期:2018-01-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cappai R,Kaslow DC,Peterson MG,Cowman AF,Anders RF,Kemp DJ

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Matrangolo FS,Liarte DB,Andrade LC,de Melo MF,Andrade JM,Ferreira RF,Santiago AS,Pirovani CP,Silva-Pereira RA,Murta SM

    更新日期:2013-08-01 00:00:00