Abstract:
:Plasmodium falciparum invades human erythrocytes by redundant pathways. Unlike Plasmodium vivax that has one Duffy Binding-Like (DBL) receptor, P. falciparum has four members of the DBL receptor family. Furthermore, one of these DBL genes, BAEBL, has polymorphisms at four amino acids in region II; each polymorphism binds to a different erythrocyte receptor. One BAEBL variant (VSTK) binds specifically to erythrocyte glycophorin C and binds poorly to neuraminidase-treated erythrocytes. When the amino acid threonine (T121) in BAEBL (VSTK) is changed to a lysine (VSKK), it no longer requires sialic acid as a receptor. To explore the molecular basis of sialic acid binding, we modeled the structure of region II of BAEBL (VSTK) on the crystal structure of a related DBL receptor, region II of erythrocyte binding antigen-175 (EBA-175). Four charged amino acids, R52, R114, E54 and D125, are predicted to surround T121 in BAEBL (VSTK). They were individually mutated to alanine (R52A, R114A, E54A, and D125A) or lysine (R52K, R114K) and expressed on the surface of Chinese hamster ovary (CHO-K1) cells. BAEBL (VSTK) with mutations in R52 or R114 of BAEBL (VSTK) bound neuraminidase-treated erythrocytes. Unlike the arginine mutations, E54A and D125A still bound poorly to neuraminidase-treated erythrocytes. These findings suggest that the two arginine residues surrounding T121 are critical for the binding specificity of BAEBL (VSTK) to sialic acid and suggest a role for arginine in sialic acid binding independent of its negative charge.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Jiang L,Duriseti S,Sun P,Miller LHdoi
10.1016/j.molbiopara.2009.06.006subject
Has Abstractpub_date
2009-11-01 00:00:00pages
49-54issue
1eissn
0166-6851issn
1872-9428pii
S0166-6851(09)00165-0journal_volume
168pub_type
杂志文章abstract::A group of Leishmania mexicana cysteine proteases that differ from those previously found in this protozoon are described. The enzymes characteristically have a preference for peptidyl substrates with a phenylalanyl-valyl-arginyl moiety, do not hydrolyse gelatin in substrate-sodium dodecyl sulphate polyacrylamide gels...
journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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更新日期:1987-07-01 00:00:00
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
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journal_title:Molecular and biochemical parasitology
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更新日期:2020-05-01 00:00:00
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更新日期:2009-05-01 00:00:00
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更新日期:1983-02-01 00:00:00
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更新日期:1987-01-02 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章,评审
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更新日期:2010-04-01 00:00:00
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更新日期:2001-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-03-01 00:00:00
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更新日期:2017-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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更新日期:2012-06-01 00:00:00
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