Glycogen metabolism in Schistosoma mansoni worms after their isolation from the host.

abstract：:Nucleotide-sugar transporters (NSTs) transport activated sugars (e.g. UDP-GlcNAc) from the cytosol to the lumen of the endoplasmic reticulum or Golgi apparatus where they are used to make glycoproteins and glycolipids. UDP-Glc is an important component of the N-glycan-dependent quality control (QC) system for protein ...

journal_title：Molecular and biochemical parasitology

authors： Banerjee S,Cui J,Robbins PW,Samuelson J

更新日期：2008-05-01 00:00:00

abstract：:During the replicative cycle of many double-stranded RNA viruses, transcription of particles with a double-stranded RNA genome alternates with replication of particles containing a single-stranded genome. In virions infecting some strains of Leishmania guyanensis the putative transcriptase and replicase activities of ...

journal_title：Molecular and biochemical parasitology

authors： Weeks RS,Patterson JL,Stuart K,Widmer G

更新日期：1992-06-01 00:00:00

abstract：:The cyclophilins are a large family of proteins implicated in folding, transport and regulation of other proteins and are potential drug targets in cancer and in some viral and parasitic infections. The functionality of cyclophilins appears to depend on peptidyl-prolyl cis-trans isomerase (foldase) and/or molecular ch...

journal_title：Molecular and biochemical parasitology

authors： Marín-Menéndez A,Monaghan P,Bell A

更新日期：2012-07-01 00:00:00

abstract：:DNA processes, including transcriptional activation, are largely governed by histone modifications. For most eukaryotes, chromatin-remodeling machines have been identified that move, replace, or decorate histones. Recent studies have shed some important light on similar machinery operating in apicomplexan parasites, i...

journal_title：Molecular and biochemical parasitology

authors： Sullivan WJ Jr,Hakimi MA

更新日期：2006-08-01 00:00:00

abstract：:A protease from Plasmodium falciparum was purified 150 fold by high performance liquid chromatography on a TSK-G-3000 SW exclusion column. The enzyme is not retained during pressure filtration with an Amicon PM10 membrane but is retained by a YM5 membrane. The molecular weight of the protease is less than 10,000, base...

journal_title：Molecular and biochemical parasitology

authors： Vander Jagt DL,Hunsaker LA,Campos NM

更新日期：1986-03-01 00:00:00

abstract：:Both patent and prepatent adult Hymenolepis diminuta excreted 20-hydroxyecdysone into the culture medium when maintained in vitro. Patent worms also excreted ecdysone and comparatively large quantities of unidentified immunoreactive material of a relatively apolar nature. This latter material was shown to be depleted ...

journal_title：Molecular and biochemical parasitology

authors： Mercer JG,Munn AE,Arme C,Rees HH

更新日期：1987-12-01 00:00:00

abstract：:The number and expression of some of the large ribosomal RNA (rRNA) gene classes present in the human malaria parasite Plasmodium falciparum was determined. Southern blot analyses, using the 5.8S rRNA coding region as a marker, indicate that the P. falciparum genome contains at least 5 distinct subclasses of large rRN...

journal_title：Molecular and biochemical parasitology

authors： Shippen-Lentz D,Afroze T,Vezza AC

更新日期：1990-01-01 00:00:00

abstract：:The influence of growth in the presence of nifurtimox on total lipids of Trypanosoma cruzi epimastigotes was determined. Nifurtimox-treated organisms showed greater amounts of unsaturated fatty acids as compared with control cells. 18:2 was the major component in the total esterified lipid fraction. Similar results we...

journal_title：Molecular and biochemical parasitology

authors： Barreto-Bergter E,Hogge L,Steele da Cruz F

更新日期：1986-12-01 00:00:00

abstract：:Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a jo...

journal_title：Molecular and biochemical parasitology

authors： Stevanovic S,Marjanović DS,Trailović SM,Zdravković N,Perdih A,Nikolic K

更新日期：2021-01-07 00:00:00

abstract：:Two cloned chromosomal segments of the hemoprotozoan Babesa bovis bearing genes from the polymorphic locus designated BabR were inserted into plasmid vector YCp19, cloned in Escherichia coli and then introduced into Saccharomyces cerevisiae. Both genes were transcribed producing poly(A+) RNAs. In at least one case, th...

journal_title：Molecular and biochemical parasitology

authors： Kemp DJ,Easton KE,Cowman AF

更新日期：1984-05-01 00:00:00

abstract：:Recent advances in insect genetic engineering have opened up new possibilities in the genetic control of insect vectors of human diseases. We outline the current state of the sterile insect technique and show how the use of engineered dominant lethals can greatly increase the effectiveness of this approach. We conside...

journal_title：Molecular and biochemical parasitology

authors： Alphey L,Andreasen M

更新日期：2002-05-01 00:00:00

abstract：:The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

journal_title：Molecular and biochemical parasitology

authors： Marshall E,Howells RE

更新日期：1985-06-01 00:00:00

abstract：:Naturally occurring sequences containing repetitive guanine motifs have the potential to form tetraplex DNA. Phytomonas serpens minicircle DNA shows some regions where one strand is composed mainly of G and T (GT regions). These regions contain several stretches of contiguous guanines. An oligonucleotide was construct...

journal_title：Molecular and biochemical parasitology

authors： Sá-Carvalho D,Traub-Cseko YM

更新日期：1995-06-01 00:00:00

abstract：:The influence of pH on the activity of purified pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) from Trypanosoma brucei has been studied. The Km for the coenzyme ADP is pH-dependent and shows the involvement of a dissociable group on the free enzyme with a pKa of 6.5-6.7. The cooperative interaction...

journal_title：Molecular and biochemical parasitology

authors： Flynn IW,Bowman IB

更新日期：1981-11-01 00:00:00

abstract：:The phylum Nematoda comprises a diverse group of roundworms that includes parasites of vertebrates, invertebrates, and plants. Human-parasitic nematodes infect more than one billion people worldwide and cause some of the most common neglected tropical diseases, particularly in low-resource countries [1]. Parasitic nem...

journal_title：Molecular and biochemical parasitology

authors： Gang SS,Hallem EA

更新日期：2016-07-01 00:00:00

abstract：:The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

journal_title：Molecular and biochemical parasitology

authors： Lindley TA,Chakraborty PR,Edlind TD

更新日期：1988-03-01 00:00:00

abstract：:The removal of N-linked oligosaccharides by peptide-N4-[N-acetyl-beta-glucoseaminyl]asparagine amidase (previously known as aspartoglycosylamine amidohydrolase and abbreviated N-glycanase) from the surface of blood or insect-transmissible forms of Trypanosoma cruzi markedly increased the capacity of these organisms to...

journal_title：Molecular and biochemical parasitology

authors： Villalta F,Kierszenbaum F

更新日期：1987-01-15 00:00:00

abstract：:Cystic hydatid disease (CHD) is caused by infection with Echinococcus granulosus metacestodes and affects humans and livestock. Proteins secreted or excreted by protoscoleces, pre-adult worms found in the metacestode, are thought to play fundamental roles in the host-parasite relationship. In this work, we performed a...

journal_title：Molecular and biochemical parasitology

authors： Virginio VG,Monteiro KM,Drumond F,de Carvalho MO,Vargas DM,Zaha A,Ferreira HB

更新日期：2012-05-01 00:00:00

abstract：:A neutralizing antiserum, C16, raised against sporozoites of Theileria parva parva was used to screen a lambda gt11 expression library of T. parva parva (Muguga) genomic DNA fragments. Proteins encoded by one phage clone, lambda TpS-17, were reactive with the C16 antiserum. Detailed characterisation of the DNA insert ...

journal_title：Molecular and biochemical parasitology

authors： Iams KP,Young JR,Nene V,Desai J,Webster P,ole-MoiYoi OK,Musoke AJ

更新日期：1990-02-01 00:00:00

abstract：:Toxoplasma gondii differentially expresses two forms of lactate dehydrogenase in tachyzoites and bradyzoites, respectively, designated LDH1 and LDH2. Previously it was demonstrated that LDH1 and LDH2 share a unique structural feature with LDH from the malarial parasite Plasmodium falciparum (pLDH), namely, the additio...

journal_title：Molecular and biochemical parasitology

authors： Dando C,Schroeder ER,Hunsaker LA,Deck LM,Royer RE,Zhou X,Parmley SF,Vander Jagt DL

更新日期：2001-11-01 00:00:00

abstract：:Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid se...

journal_title：Molecular and biochemical parasitology

authors： Eakin AE,Bouvier J,Sakanari JA,Craik CS,McKerrow JH

更新日期：1990-02-01 00:00:00

abstract：:The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

journal_title：Molecular and biochemical parasitology

authors： Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

更新日期：1988-02-01 00:00:00

abstract：:Gene expression and its regulation was studied in the rat tapeworm, Hymenolepis diminuta, during strobilization. RNAs extracted from the different developmental stages of the parasite were translated in vitro in a rabbit reticulocyte lysate system. Two-dimensional patterns of the translational products were compared w...

journal_title：Molecular and biochemical parasitology

authors： Siddiqui AA,Karcz SR,Podesta RB

更新日期：1987-08-01 00:00:00

abstract：:We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

journal_title：Molecular and biochemical parasitology

authors： Dissanayake S,Xu M,Piessens WF

更新日期：1992-12-01 00:00:00

abstract：:Kinetoplast DNA (kDNA) was isolated from 56 stocks of Trypanosoma cruzi isolated from human patients, animals and insects from Brazil, Venezuela, Colombia and Costa Rica. Comparison of the patterns of digested kDNA on acrylamide gels led to the grouping of several stocks into two schizodemes. Schizodeme analysis was a...

journal_title：Molecular and biochemical parasitology

authors： Avila H,Goncalves AM,Nehme NS,Morel CM,Simpson L

更新日期：1990-09-01 00:00:00

abstract：:We have cloned and expressed the full-length gene encoding the hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) from the anaerobic protozoan parasite Tritrichomonas foetus. This enzyme is essential in nucleic acid metabolism of T. foetus because the parasite is unable to synthesize purine nucleotide...

journal_title：Molecular and biochemical parasitology

authors： Chin MS,Wang CC

更新日期：1994-02-01 00:00:00

abstract：:Cathepsin L-like activity was demonstrated in the excretory/secretory (E/S) products of Fasciola hepatica newly excysted juveniles (NEJ), 3-week-old, 5-week-old and mature flukes using the fluorogenic substituted 7-amino-4-methylcoumarin substrates Z-phe-arg-AMC, Z-arg-arg-AMC and Z-arg-AMC. Gelatin-substrate polyacry...

journal_title：Molecular and biochemical parasitology

authors： Carmona C,Dowd AJ,Smith AM,Dalton JP

更新日期：1993-11-01 00:00:00

abstract：:Two different classes of small nematode specific lipid-binding proteins, the nematode polyprotein allergens/antigens (NPAs) and the fatty acid- and retinol-binding (FAR) proteins, are secreted by helminth parasites. Until now, there was no evidence of the expression or secretion of these two families of proteins in Ha...

journal_title：Molecular and biochemical parasitology

authors： Kuang L,Colgrave ML,Bagnall NH,Knox MR,Qian M,Wijffels G

更新日期：2009-11-01 00:00:00

abstract：:The glycosylphosphatidylinositol phospholipase C (GPI-PLC) from Trypanosoma brucei is particularly effective in hydrolysing the GPI-anchors of some proteins. The enzyme is inhibited by Zn2+ and p-chloromercurylphenylsulphonic acid, both of which can act as sulphydryl reagents, suggesting that a cysteine residue may be...

journal_title：Molecular and biochemical parasitology

authors： Carnall N,Webb H,Carrington M

更新日期：1997-12-15 00:00:00

abstract：:The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC 3.6.1.5) which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

journal_title：Molecular and biochemical parasitology

authors： Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

更新日期：2008-12-01 00:00:00