Some kinetic properties of pyruvate kinase from Trypanosoma brucei: influence of pH and fructose-1,6-diphosphate.

abstract：:An NADH-cytochrome c reductase (complex I-III) was isolated from Ascaris suum muscle mitochondria. The enzyme preparation catalyzed the reduction of 1.68 mumol cytochrome c min-1 mg-1 protein at 25 degrees C with NADH but not with NADPH, and retained its sensitivity to rotenone, piericidin A and 2-heptyl-4-hydroxyquin...

journal_title：Molecular and biochemical parasitology

authors： Takamiya S,Furushima R,Oya H

更新日期：1984-10-01 00:00:00

abstract：:The major merozoite-piroplasm surface antigen (mMPSA) of Theileria annulata, Tams1, is known to be antigenically diverse. The possession of variable N-linked glycosylation sites and removal of monoclonal antibody 5E1 reactivity by mild periodate treatment suggested, previously, that divergent epitopes may be conferred...

journal_title：Molecular and biochemical parasitology

authors： Katzer F,McKellar S,Ferguson MA,d'Oliveira C,Shiels BR

更新日期：2002-06-01 00:00:00

abstract：:The guide RNA (gRNA) paradigm states that the uridine (U) insertion/deletion type of RNA editing is mediated by short 3' uridylylated gRNAs that are complementary to specific blocks of mature edited sequence. These gRNAs contain the edited sequence information in the form of guiding purine residues that can base pair ...

journal_title：Molecular and biochemical parasitology

authors： Simpson L

更新日期：1997-06-01 00:00:00

abstract：:Antigenic switching in Trypanosoma brucei can occur either by the production of a telomeric copy of a variant surface glycoprotein (VSG) gene through a gene conversion mechanism or by the nonduplicative activation of a telomeric VSG gene. The 5 VSG gene telomeric copy that is expressed in IsTaR 1 variant antigenic typ...

journal_title：Molecular and biochemical parasitology

authors： Aline RF Jr,Stuart K

更新日期：1985-06-01 00:00:00

abstract：:The presence of virus-derived RNA was investigated in 38 axenically growing Giardia isolates from different geographic areas. The RNA virus was demonstrated in Giardia strains from humans in the U.S.A., England and the majority of strains from Poland. Two strains isolated respectively from a cat and a cavia also conta...

journal_title：Molecular and biochemical parasitology

authors： De Jonckheere JF,Gordts B

更新日期：1987-02-01 00:00:00

abstract：:We have determined the sequence of a cDNA (Tt34c1) encoding a Trypanosoma cruzi trypomastigote stage-specific 85-kDa surface glycoprotein (gp85). Within the peptide sequence of Tt34c1 are two 8-amino acid motifs, Ser-X-Asp-X-Gly-X-Thr-Trp, that are characteristic of bacterial neuraminidases. Analysis of the Tt34c1 seq...

journal_title：Molecular and biochemical parasitology

authors： Takle GB,Cross GA

更新日期：1991-10-01 00:00:00

abstract：:A protease from Plasmodium falciparum was purified 150 fold by high performance liquid chromatography on a TSK-G-3000 SW exclusion column. The enzyme is not retained during pressure filtration with an Amicon PM10 membrane but is retained by a YM5 membrane. The molecular weight of the protease is less than 10,000, base...

journal_title：Molecular and biochemical parasitology

authors： Vander Jagt DL,Hunsaker LA,Campos NM

更新日期：1986-03-01 00:00:00

abstract：:Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) are the main membrane phospholipids (PLs) of Plasmodium parasites and can be generated by the de novo (Kennedy) CDP-choline and CDP-ethanolamine pathways and by the CDP-diacylglycerol dependent pathway. The Kennedy pathways initiate from exogenous choline and ...

journal_title：Molecular and biochemical parasitology

authors： Déchamps S,Wengelnik K,Berry-Sterkers L,Cerdan R,Vial HJ,Gannoun-Zaki L

更新日期：2010-10-01 00:00:00

abstract：:A gene encoding the small subunit rRNA (SSUrRNA) has been isolated from the human parasite, Plasmodium malariae. The gene has been sequenced. It contains conserved and variable regions which conform to patterns established for other eukaryotic SSUrRNA genes. Comparisons with other SSUrRNA genes from Plasmodium species...

journal_title：Molecular and biochemical parasitology

authors： Goman M,Mons B,Scaife J

更新日期：1991-04-01 00:00:00

abstract：:EP and GPEET procyclins are the major surface glycoproteins of Trypanosoma brucei in the midgut of tsetse flies (Glossina spp.). The procyclin genes are located at the beginning of polycistronic transcription units and are followed by at least one procyclin-associated gene (PAG). The EP/PAG1 locus on one copy of chrom...

journal_title：Molecular and biochemical parasitology

authors： Haenni S,Renggli CK,Fragoso CM,Oberle M,Roditi I

更新日期：2006-12-01 00:00:00

abstract：:Release of sporozoites from Eimeria oocysts/sporocysts is an essential step in the intracellular development of the parasite in its host. Little is known about this process except that elevated temperature (∼ 40 °C) plus trypsin and bile salts are required for sporozoite to escape from sporocysts. In this study, it wa...

journal_title：Molecular and biochemical parasitology

authors： Jenkins MC,O'Brien CN,Parker C

更新日期：2019-10-01 00:00:00

abstract：:The 5' and 3' regions adjacent to the initiation codon in 22 Plasmodium falciparum sequences were examined. A 5' consensus sequence (AAAA/ATG) was found. Although P. falciparum non-translated DNA is A-rich, A occurred significantly more frequently in the 4 positions preceding the initiation ATG than in adjacent non-tr...

journal_title：Molecular and biochemical parasitology

authors： Saul A,Battistutta D

更新日期：1990-08-01 00:00:00

abstract：:A group of Leishmania mexicana cysteine proteases that differ from those previously found in this protozoon are described. The enzymes characteristically have a preference for peptidyl substrates with a phenylalanyl-valyl-arginyl moiety, do not hydrolyse gelatin in substrate-sodium dodecyl sulphate polyacrylamide gels...

journal_title：Molecular and biochemical parasitology

authors： Robertson CD,Coombs GH

更新日期：1993-12-01 00:00:00

abstract：:A Plasmodium falciparum gene closely linked to the chloroquine resistance locus encodes PfCG2, a predicted 320-330kDa protein. In the parasitized erythrocyte, PfCG2 expression rises sharply in the trophozoite stage and is detected in electron-dense patches along the parasitophorous vacuolar membrane (PVM), in the cyto...

journal_title：Molecular and biochemical parasitology

authors： Cooper RA,Papakrivos J,Lane KD,Fujioka H,Lingelbach K,Wellems TE

更新日期：2005-12-01 00:00:00

abstract：:This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose con...

journal_title：Molecular and biochemical parasitology

authors： Horemans AM,Tielens AG,van den Bergh SG

更新日期：1992-03-01 00:00:00

abstract：:Peptidomimetic inhibitors of mammalian zinc metalloproteases have been tested as potential agents for intervention in disease caused by kinetoplastid protozoa. Certain metalloprotease inhibitors were able to inhibit the release of variant surface glycoprotein from cultured transgenic procyclic Trypanosoma brucei, conf...

journal_title：Molecular and biochemical parasitology

authors： Bangs JD,Ransom DA,Nimick M,Christie G,Hooper NM

更新日期：2001-04-25 00:00:00

abstract：:The cytotoxicity of ricin and diphtheria toxin was studied in culture-adapted bloodstream forms of Trypanosoma brucei. Although ricin is endocytosed at a rate comparable to that of other internalized macromolecules, it is nontoxic to bloodstream-form trypanosomes. The resistance lies partly in low susceptibility of th...

journal_title：Molecular and biochemical parasitology

authors： Scory S,Steverding D

更新日期：1997-12-01 00:00:00

abstract：:Several proteins synthesized by mature asexual stages of Plasmodium falciparum interact with the erythrocyte membrane skeleton. One of these is the mature-parasite-infected erythrocyte surface antigen (MESA; also called PfEMP2), a phosphoprotein of 250-300 kDa, which is found on the internal face of the erythrocyte me...

journal_title：Molecular and biochemical parasitology

authors： Lustigman S,Anders RF,Brown GV,Coppel RL

更新日期：1990-01-15 00:00:00

abstract：:DNA processes, including transcriptional activation, are largely governed by histone modifications. For most eukaryotes, chromatin-remodeling machines have been identified that move, replace, or decorate histones. Recent studies have shed some important light on similar machinery operating in apicomplexan parasites, i...

journal_title：Molecular and biochemical parasitology

authors： Sullivan WJ Jr,Hakimi MA

更新日期：2006-08-01 00:00:00

abstract：:The major proteolytic activity of Trypanosoma cruzi is a cathepsin L-like cysteine protease expressed in all stages of the parasite. As an initial step in identifying possible functions of this enzyme in the life cycle of T. cruzi, and examining its potential as a target for rational drug design, two fluoromethyl keto...

journal_title：Molecular and biochemical parasitology

authors： Harth G,Andrews N,Mills AA,Engel JC,Smith R,McKerrow JH

更新日期：1993-03-01 00:00:00

abstract：:Glucose is a major source of energy and carbon in promastigotes of Leishmania mexicana, and its uptake is mediated by three glucose transporters whose genes are encoded within a single cluster. A null mutant in which the glucose transporter gene cluster was deleted by homologous gene replacement was generated previous...

journal_title：Molecular and biochemical parasitology

authors： Rodriguez-Contreras D,Feng X,Keeney KM,Bouwer HG,Landfear SM

更新日期：2007-05-01 00:00:00

abstract：:The alpha and beta tubulin genes of Leishmania major were cloned and used to study the genomic organization, chromosomal location and transcription of tubulin genes in L. major. The number of beta tubulin isogenes was determined by hybridization of probes representing the 5' and 3' ends of the cloned beta tubulin cDNA...

journal_title：Molecular and biochemical parasitology

authors： Spithill TW,Samaras N

更新日期：1987-05-01 00:00:00

abstract：:Paramyosin of Clonorchis sinensis (CsPmy) is a myofibrillar protein localized in subtegumental muscle, tegument, and the muscle layer surrounding the intestine of the parasite. Previously, we have identified that CsPmy reacted with sera of human clonorchiasis and this protein had a potential as a candidate antigen for...

journal_title：Molecular and biochemical parasitology

authors： Kang JM,Ju HL,Lee J,Kim TI,Cho SH,Kim TS,Sohn WM,Na BK

更新日期：2015-05-01 00:00:00

abstract：:A mild surface-labeling procedure was applied to various developmental stages of Schistosoma mansoni. An 18 kDa protein was preferentially labeled in freshly transformed schistosomula. The labeled protein was equally present on skin-penetrated and mechanically prepared schistosomula and it disappeared upon digestion o...

journal_title：Molecular and biochemical parasitology

authors： Liberti P,Festucci A,Ruppel A,Gigante S,Cioli D

更新日期：1986-01-01 00:00:00

abstract：:The tachyzoites and the tissue cysts containing bradyzoites of Neospora caninum and Toxoplasma gondii, respectively, are difficult to distinguish morphologically. Specific antigens have been identified in T. gondii tachyzoites and bradyzoites, some of which are stage-specifically expressed, and different functions hav...

journal_title：Molecular and biochemical parasitology

authors： Sonda S,Fuchs N,Connolly B,Fernandez P,Gottstein B,Hemphill A

更新日期：1998-11-30 00:00:00

abstract：:Survival of Onchocerca volvulus, a pathogenic human filarial parasite, is likely to depend upon the detoxification activities of the glutathione S-transferases (GSTs). The 24 kDa O. volvulus GST, OvGST2, was expressed in a bacterial system and the recombinant protein was purified to homogeneity by affinity chromatogra...

journal_title：Molecular and biochemical parasitology

authors： Liebau E,Wildenburg G,Brophy PM,Walter RD,Henkle-Dührsen K

更新日期：1996-09-01 00:00:00

abstract：:Plasmodium falciparum is the causative organism for the most severe form of malaria among humans. The clinical symptoms are accredited to the asexual stage of parasite life cycle, involving merozoite invasion of erythrocyte, development and re-invasion into the new erythrocyte. Interaction of parasite proteins present...

journal_title：Molecular and biochemical parasitology

authors： Hans N,Relan U,Dubey N,Gaur D,Chauhan VS

更新日期：2015-08-01 00:00:00

abstract：:Sulfated glycosphingolipids are present on the surface of a variety of cells. They are active participants in adhesion processes in many systems and appear to be involved in the regulation of cell proliferation, differentiation and other developmental cellular events. However, the body of knowledge about synthesis, st...

journal_title：Molecular and biochemical parasitology

authors： Landoni M,Duschak VG,Peres VJ,Nonami H,Erra-Balsells R,Katzin AM,Couto AS

更新日期：2007-07-01 00:00:00

abstract：:Beta 1,3-glucan recognition proteins (GRP) have specific affinity for beta 1,3-glucan, a component on the surface of fungi and bacteria. By interacting with beta 1,3-glucan, GRP initiates activation of prophenoloxidase, a key enzyme in the signaling pathway leading to melanotic encapsulation in invertebrates. In this ...

journal_title：Molecular and biochemical parasitology

authors： Wang X,Fuchs JF,Infanger LC,Rocheleau TA,Hillyer JF,Chen CC,Christensen BM

更新日期：2005-01-01 00:00:00

abstract：:Chemical modifications of Class I aldolases from Trypanosoma brucei, rabbit muscle and Staphylococcus aureus with carboxypeptidase A, glyceraldehyde 3-phosphate and cysteine-specific reagents revealed the following differences between the three homologous enzymes. Aldolase from S. aureus was not affected by any of the...

journal_title：Molecular and biochemical parasitology

authors： Callens M,Opperdoes FR

更新日期：1991-07-01 00:00:00