Some kinetic properties of pyruvate kinase from Trypanosoma brucei: influence of pH and fructose-1,6-diphosphate.


:The influence of pH on the activity of purified pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC from Trypanosoma brucei has been studied. The Km for the coenzyme ADP is pH-dependent and shows the involvement of a dissociable group on the free enzyme with a pKa of 6.5-6.7. The cooperative interaction of the multiple phosphoenolpyruvate (PEP) binding sites is independent of pH in the range of 5.7-7.8. Variation of the Vmax value with pH indicates the presence of a dissociated group (pKa 6.2-6.3) and of an undissociated group (pKa 7.5-7.6) in the enzyme-substrate complex. A doubly dissociated phosphate group on PEP is shown to be essential by the effects of pH on the S0.5 value for this substrate, as is an undissociated enzyme group with a pKa in the range 6.7-7.0. It is shown that PEP and frucotse-1,6-diphosphate (FDP) act entirely in conjunction in allosterically activating the enzyme, FDP, the heterotropic effector, decreases the interaction between PEP binding sites at low concentration, and decreases the S0.5 value for PEP at higher concentration. A model for the interaction of the enzyme with its substrates is discussed.


Mol Biochem Parasitol


Flynn IW,Bowman IB




Has Abstract


1981-11-01 00:00:00














  • Context-dependent roles of the Entamoeba histolytica core promoter element GAAC in transcriptional activation and protein complex assembly.

    abstract::Transcriptional control of the hgl5 gene of Entamoeba histolytica is mediated through an unusual core promoter composed of TATA, GAAC and Initiator elements. In the hgl5 promoter the GAAC element (AATGAACT) determines the site and rate of transcription initiation. Here we tested the role of the GAAC element in transcr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Singh U,Gilchrist CA,Schaenman JM,Rogers JB,Hockensmith JW,Mann BJ,Petri WA

    更新日期:2002-03-01 00:00:00

  • Transgenic Plasmodium berghei sporozoites expressing beta-galactosidase for quantification of sporozoite transmission.

    abstract::Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Engelmann S,Sinnis P,Matuschewski K

    更新日期:2006-03-01 00:00:00

  • Mapping of the putative epitope domain of Clonorchis sinensis paramyosin (CsPmy) recognized by CsPmy-specific immunoglobulin G in sera of human clonorchiasis.

    abstract::Paramyosin of Clonorchis sinensis (CsPmy) is a myofibrillar protein localized in subtegumental muscle, tegument, and the muscle layer surrounding the intestine of the parasite. Previously, we have identified that CsPmy reacted with sera of human clonorchiasis and this protein had a potential as a candidate antigen for...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kang JM,Ju HL,Lee J,Kim TI,Cho SH,Kim TS,Sohn WM,Na BK

    更新日期:2015-05-01 00:00:00

  • Molecular characterisation of an expressed sequence tag locus of Toxoplasma gondii encoding the micronemal protein MIC2.

    abstract::The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wan KL,Carruthers VB,Sibley LD,Ajioka JW

    更新日期:1997-02-01 00:00:00

  • The merozoite surface protein 6 gene codes for a 36 kDa protein associated with the Plasmodium falciparum merozoite surface protein-1 complex.

    abstract::A complex of non-covalently bound polypeptides is located on the surface of the merozoite form of the human malaria parasite Plasmodium falciparum. Four of these polypeptides are derived by proteolytic processing of the merozoite surface protein 1 (MSP-1) precursor. Two components, a 22 and a 36 kDa polypeptide are no...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Trucco C,Fernandez-Reyes D,Howell S,Stafford WH,Scott-Finnigan TJ,Grainger M,Ogun SA,Taylor WR,Holder AA

    更新日期:2001-01-15 00:00:00

  • The ecdysone receptor (EcR) is a major regulator of tissue development and growth in the marine salmonid ectoparasite, Lepeophtheirus salmonis (Copepoda, Caligidae).

    abstract::The function of the ecdysone receptor (EcR) during development and molting has been thoroughly investigated in some arthropods such as insects but rarely in crustacean copepods such as the salmon louse Lepeophtheirus salmonis (L. salmonis) (Copepoda, Caligidae). The salmon louse is an ectoparasite on Atlantic salmon t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sandlund L,Nilsen F,Male R,Dalvin S

    更新日期:2016-08-01 00:00:00

  • A diamidine-resistant Trypanosoma equiperdum clone contains a P2 purine transporter with reduced substrate affinity.

    abstract::Following the demonstration that the transport of melaminophenyl arsenical drugs in Trypanosoma brucei is dependent upon an unusual adenosine nucleoside transporter (Carter and Fairlamb, Nature 361 (1993) 173-175) we have investigated adenosine transport in the related parasite Trypanosoma equiperdum (Botat1.1) and a ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barrett MP,Zhang ZQ,Denise H,Giroud C,Baltz T

    更新日期:1995-07-01 00:00:00

  • Mechanisms of respiration and phosphorylation in Ascaris muscle mitochondria.

    abstract::In Ascaris muscle mitochondria the major respiratory chain-linked phosphorylation activity is accomplished by a NADH-linked reduction of fumarate to succinate. Oxygen can also be employed as a terminal electron acceptor via a cyanide- and salicyl-hydroxamate-resistant terminal oxidase. As in fumarate-dependent electro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Köhler P,Bachmann R

    更新日期:1980-04-01 00:00:00

  • Actin sequestering protein, profilin, regulates intracellular vesicle transport in Leishmania.

    abstract::Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM

    更新日期:2020-07-01 00:00:00

  • Antimalarial action of nitrobenzylthioinosine in combination with purine nucleoside antimetabolites.

    abstract::The infection of human erythrocytes by two strains of the human malarial parasite, Plasmodium falciparum (FCQ-27 or the multi-drug-resistant strain K-1), markedly changed the transport characteristics of the nucleosides, adenosine and tubercidin, compared to uninfected erythrocytes. A component of the transport of the...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gero AM,Scott HV,O'Sullivan WJ,Christopherson RI

    更新日期:1989-04-01 00:00:00

  • Peroxisomes in parasitic protists.

    abstract::Representatives of all major lineages of eukaryotes contain peroxisomes with similar morphology and mode of biogenesis, indicating a monophyletic origin of the organelles within the common ancestor of all eukaryotes. Peroxisomes originated from the endoplasmic reticulum, but despite a common origin and shared morpholo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Gabaldón T,Ginger ML,Michels PA

    更新日期:2016-01-01 00:00:00

  • Characterization of trafficking pathways and membrane genesis in malaria-infected erythrocytes.

    abstract::The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pouvelle B,Gormley JA,Taraschi TF

    更新日期:1994-07-01 00:00:00

  • Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues.

    abstract::The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cameron S,Fyffe SA,Goldie S,Hunter WN

    更新日期:2008-04-01 00:00:00

  • Two-dimensional electrophoretic separation and fluorographic analysis of the gene products synthesized by Hymenolepis diminuta with particular reference to the parasite-specific polypeptides in the brush border membrane.

    abstract::This study is the first demonstration of individual gene products synthesized by Hymenolepis diminuta. To identify proteins synthesized specifically by H. diminuta, we radiolabeled adult worms with [14C]leucine in vitro. Subsequent one- and two-dimensional gel electrophoretic separation and staining of the protein con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Atkinson BG,Podesta RB

    更新日期:1982-07-01 00:00:00

  • Molecular cloning and characterisation of a developmentally regulated putative metallopeptidase present in a host protective extract of Haemonchus contortus.

    abstract::Antisera from lambs immunised with the Haemonchus contortus integral membrane protein complex, Haemonchus galactose-containing glycoprotein (H-gal-GP), the lambs being refractory to subsequent challenge, were used to identify several clones from an adult H. contortus lambda gt11 cDNA library. Using gene-specific oligo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Redmond DL,Knox DP,Newlands G,Smith WD

    更新日期:1997-03-01 00:00:00

  • Molecular and functional characterisation of the heat shock protein 10 of Strongyloides ratti.

    abstract::Strongyloides stercoralis and S. ratti are intestinal parasitic nematodes infecting rats and humans, respectively. Both present extraordinary life cycles comprising a free-living generation in addition to parasitic stages. In search of molecules possibly involved in parasite-host interaction, we performed mass spectro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tazir Y,Steisslinger V,Soblik H,Younis AE,Beckmann S,Grevelding CG,Steen H,Brattig NW,Erttmann KD

    更新日期:2009-12-01 00:00:00

  • Characterization of beta-tubulin genes in hookworms and investigation of resistance-associated mutations using real-time PCR.

    abstract::Human hookworms (Ancylostoma duodenale, Necator americanus) are a major cause of malnutrition and anemia, particularly in children, and high worm burdens can lead to stunted growth and mental retardation. Mass drug administration (MDA) with benzimidazole (BZ) anthelmintics has the potential to greatly reduce morbidity...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schwenkenbecher JM,Albonico M,Bickle Q,Kaplan RM

    更新日期:2007-12-01 00:00:00

  • Pyrazolopyrimidine metabolism in African trypanosomes: metabolic similarities to Trypanosoma cruzi and Leishmania spp.

    abstract::Growth inhibition and radioisotope incorporation studies with allopurinol (4-hydroxypyrazolo(3,4-d)pyrimidine) have shown that the African trypanosomes are biologically and biochemically similar to Leishmania spp. and Trypanosoma cruzi with respect to their response to this compound. These organisms, as a group, share...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Berens RL,Marr JJ,Brun R

    更新日期:1980-04-01 00:00:00

  • Molecules on the surface of the Plasmodium falciparum infected erythrocyte and their role in malaria pathogenesis and immune evasion.

    abstract::The surface of the erythrocyte undergoes a number of modifications during infection by Plasmodium falciparum. These modifications are critical for pathogenesis of severe disease and the acquisition of host immunity through their role in interactions between the host and the parasite and in antigenic variation. Our kno...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Craig A,Scherf A

    更新日期:2001-07-01 00:00:00

  • Accurate transcription of a cloned gene from Babesia bovis in Saccharomyces cerevisiae.

    abstract::Two cloned chromosomal segments of the hemoprotozoan Babesa bovis bearing genes from the polymorphic locus designated BabR were inserted into plasmid vector YCp19, cloned in Escherichia coli and then introduced into Saccharomyces cerevisiae. Both genes were transcribed producing poly(A+) RNAs. In at least one case, th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kemp DJ,Easton KE,Cowman AF

    更新日期:1984-05-01 00:00:00

  • An exported protein of Plasmodium falciparum is synthesized as an integral membrane protein.

    abstract::Exp-1 is an antigen of Plasmodium falciparum which is transported from the parasite cell to the membrane of the parasitophorous vacuole and to membranous compartments in the erythrocyte. To investigate how this protein is transported, we studied the synthesis and membrane translocation of exp-1 in a cell-free system. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Günther K,Tümmler M,Arnold HH,Ridley R,Goman M,Scaife JG,Lingelbach K

    更新日期:1991-05-01 00:00:00

  • Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.

    abstract::The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the struct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chen M,Li E,Stanley SL Jr

    更新日期:2004-10-01 00:00:00

  • In vivo 31P NMR spectrum of Hymenolepis diminuta and its change on short-term exposure to mebendazole.

    abstract::The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Thompson SN,Platzer EG,Lee RW

    更新日期:1987-01-02 00:00:00

  • In vitro selection of halofantrine resistance in Plasmodium falciparum is not associated with increased expression of Pgh1.

    abstract::Recent investigations into quinoline and phenanthrene methanol resistance in Plasmodium falciparum have described a linkage between amplification of the mdr homologue pfmdr1 and decreased susceptibility to mefloquine (MQ) and halofantrine (HF). We have examined the current theories on cross-resistance patterns and pfm...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ritchie GY,Mungthin M,Green JE,Bray PG,Hawley SR,Ward SA

    更新日期:1996-12-02 00:00:00

  • E. dispar strain: analysis of polymorphism as a tool for study of geographic distribution.

    abstract::The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ramos F,García G,Valadez A,Morán P,González E,Gómez A,Melendro EI,Valenzuela O,Ximénez C

    更新日期:2005-06-01 00:00:00

  • An evaluation of different methods for labelling the surface of the filarial nematode Brugia pahangi with 125iodine.

    abstract::The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marshall E,Howells RE

    更新日期:1985-06-01 00:00:00

  • RNA primer removal and gap filling on a model minicircle replication intermediate.

    abstract::Replication of kinetoplast DNA minicircles in Crithidia fasciculata occurs by a unidirectional mechanism involving continuous synthesis of one strand (L strand) and discontinuous synthesis of the complementary strand (H strand). L-strands are initiated by RNA priming at alternate origins (A and B) resulting in daughte...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hines JC,Engel ML,Zhao H,Ray DS

    更新日期:2001-06-01 00:00:00

  • Substrate specificity studies of the cysteine peptidases falcipain-2 and falcipain-3 from Plasmodium falciparum and demonstration of their kininogenase activity.

    abstract::We studied the substrate specificity requirements of recombinant cysteine peptidases from Plasmodium falciparum, falcipain-2 (FP-2) and falcipain-3 (FP-3), using fluorescence resonance energy transfer (FRET) peptides as substrates. Systematic modifications were introduced in the lead sequence Abz-KLRSSKQ-EDDnp (Abz=or...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cotrin SS,Gouvêa IE,Melo PM,Bagnaresi P,Assis DM,Araújo MS,Juliano MA,Gazarini ML,Rosenthal PJ,Juliano L,Carmona AK

    更新日期:2013-02-01 00:00:00

  • DNA sequence of Crithidia fasciculata kinetoplast minicircles.

    abstract::Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sugisaki H,Ray DS

    更新日期:1987-04-01 00:00:00

  • Cloning and functional identification of a novel annexin subfamily in Cysticercus cellulosae.

    abstract::A novel cDNA was isolated from a library of Cysticercus cellulosae by immunological screening. Sequence analysis reveals that it has typical annexin structures. However, since its N-terminus is distinct and the core domain only has 32-44% amino acid identity to the tetrads of other annexins, the cDNA encodes a member ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hongli Y,Shuhan S,Ruiwen C,Yingjun G

    更新日期:2002-01-01 00:00:00