Base J glucosyltransferase does not regulate the sequence specificity of J synthesis in trypanosomatid telomeric DNA.


:Telomeric DNA of trypanosomatids possesses a modified thymine base, called base J, that is synthesized in a two-step process; the base is hydroxylated by a thymidine hydroxylase forming hydroxymethyluracil (hmU) and a glucose moiety is then attached by the J-associated glucosyltransferase (JGT). To examine the importance of JGT in modifiying specific thymine in DNA, we used a Leishmania episome system to demonstrate that the telomeric repeat (GGGTTA) stimulates J synthesis in vivo while mutant telomeric sequences (GGGTTT, GGGATT, and GGGAAA) do not. Utilizing an in vitro GT assay we find that JGT can glycosylate hmU within any sequence with no significant change in Km or kcat, even mutant telomeric sequences that are unable to be J-modified in vivo. The data suggests that JGT possesses no DNA sequence specificity in vitro, lending support to the hypothesis that the specificity of base J synthesis is not at the level of the JGT reaction.


Mol Biochem Parasitol


Bullard W,Cliffe L,Wang P,Wang Y,Sabatini R




Has Abstract


2015-12-01 00:00:00














  • Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum: different logics achieve the same goal.

    abstract::Babesia bovis is a protozoal hemoparasite of cattle which behaves in certain crucial respects like Plasmodium falciparum, despite being phylogenetically distant and having many differences in its life cycle. The shared behavioral attributes of rapid antigenic variation and cytoadhesion/sequestration are thought to con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Allred DR,Al-Khedery B

    更新日期:2004-03-01 00:00:00

  • Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.

    abstract::The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the struct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chen M,Li E,Stanley SL Jr

    更新日期:2004-10-01 00:00:00

  • The effect of over-expression of the alternative oxidase in the procyclic forms of Trypanosoma brucei.

    abstract::Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Walker R Jr,Saha L,Hill GC,Chaudhuri M

    更新日期:2005-02-01 00:00:00

  • Characterization of a putative nuclear receptor from Onchocerca volvulus.

    abstract::Steroids and retinoids are important regulators of development in invertebrates and vertebrates. The central mediators of action of these compounds are their cognate receptors, which together form a family of proteins known as the nuclear receptor family. Previous studies have demonstrated that the genome of Onchocerc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Unnasch TR,Bradley J,Beauchamp J,Tuan R,Kennedy MW

    更新日期:1999-11-30 00:00:00

  • Evolution of the microtubular cytoskeleton (flagellar apparatus) in parasitic protists.

    abstract::The microtubular cytoskeleton of most single-celled eukaryotes radiates from an organizing center called the flagellar apparatus, which is essential for locomotion, feeding and reproduction. The structure of the flagellar apparatus tends to be conserved within diverse clades of eukaryotes, and modifications of this ov...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Yubuki N,Čepička I,Leander BS

    更新日期:2016-01-01 00:00:00

  • Efficient extraction and translation of Plasmodium falciparum messenger RNA.

    abstract::A convenient method was employed for the efficient extraction of total RNA from Plasmodium falciparum. By depleting the total RNA of tRNA, it was shown that P. falciparum or P. lophurae tRNAs markedly stimulate the translation of P. falciparum mRNA in the rabbit reticulocyte cell-free system. Analysis of the cell-free...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wallach M

    更新日期:1982-12-01 00:00:00

  • Control of gene expression in Plasmodium falciparum.

    abstract::Transfection has facilitated a functional analysis of transcriptional processes in the human malarial parasite Plasmodium falciparum, providing the first fascinating glimpses into the mechanisms regulating parasite development and pathogenicity. Here we review our rapidly evolving knowledge of what constitutes a promo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Horrocks P,Dechering K,Lanzer M

    更新日期:1998-09-15 00:00:00

  • Arp1, an actin-related protein, in Plasmodium berghei.

    abstract::Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Siden-Kiamos I,Schüler H,Liakopoulos D,Louis C

    更新日期:2010-10-01 00:00:00

  • Transient expression of luciferase in Entamoeba histolytica driven by the ferredoxin gene 5' and 3' regions.

    abstract::We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gilchrist CA,Streets HL,Ackers JP,Hall R

    更新日期:1995-10-01 00:00:00

  • Silencing of a putative inner arm dynein heavy chain results in flagellar immotility in Trypanosoma brucei.

    abstract::The Trypanosoma brucei flagellum controls motility and is crucial for cell polarity and division. Unique features of trypanosome motility suggest that flagellar beat regulation in this organism is unusual and worthy of study. The flagellar axoneme, required for motility, has a structure that is highly conserved among ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Springer AL,Bruhn DF,Kinzel KW,Rosenthal NF,Zukas R,Klingbeil MM

    更新日期:2011-01-01 00:00:00

  • Plasmodium falciparum biosynthesizes sulfoglycosphingolipids.

    abstract::Sulfated glycosphingolipids are present on the surface of a variety of cells. They are active participants in adhesion processes in many systems and appear to be involved in the regulation of cell proliferation, differentiation and other developmental cellular events. However, the body of knowledge about synthesis, st...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Landoni M,Duschak VG,Peres VJ,Nonami H,Erra-Balsells R,Katzin AM,Couto AS

    更新日期:2007-07-01 00:00:00

  • Characterization and localization of Plasmodium falciparum homolog of prokaryotic ClpQ/HslV protease.

    abstract::The beta subunits (beta1, beta2, and beta5) of 20S proteasome and HslV/ClpQ are ATP-dependent threonine proteases present in eukaryotes and prokaryotes, respectively that control levels of key regulatory proteins in the cell. The orthologue of prokaryotic HslV protease in Plasmodium falciparum (PfHslV) is a novel drug...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ramasamy G,Gupta D,Mohmmed A,Chauhan VS

    更新日期:2007-04-01 00:00:00

  • An evaluation of different methods for labelling the surface of the filarial nematode Brugia pahangi with 125iodine.

    abstract::The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marshall E,Howells RE

    更新日期:1985-06-01 00:00:00

  • Benznidazole-resistance in Trypanosoma cruzi: evidence that distinct mechanisms can act in concert.

    abstract::Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Campos MC,Leon LL,Taylor MC,Kelly JM

    更新日期:2014-01-01 00:00:00

  • Transgenic Plasmodium berghei sporozoites expressing beta-galactosidase for quantification of sporozoite transmission.

    abstract::Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Engelmann S,Sinnis P,Matuschewski K

    更新日期:2006-03-01 00:00:00

  • Molecular characterization and expression of two putative protective excretory secretory proteins of Haemonchus contortus.

    abstract::It has been shown that vaccination with two low molecular mass excretory secretory (ES) antigens of 15 and 24 kDa, respectively, afforded a substantial degree of protection against Haemonchus contortus to sheep. In vitro cultivation of the parasite usually yields a limited amount of these proteins and therefore, recom...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schallig HD,van Leeuwen MA,Verstrepen BE,Cornelissen AW

    更新日期:1997-09-01 00:00:00

  • Identification and characterization of a Plasmodium falciparum RNA polymerase gene with similarity to mitochondrial RNA polymerases.

    abstract::Nearly all mitochondrial RNA polymerase genes identified to date are encoded in the nucleus and have similarities to T3 and T7 bacteriophage RNA polymerases. Some chloroplast genes are also transcribed by T3/T7 phage-like RNA polymerases, raising the possibility that the apicomplexan parasites, which have both a mitoc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Li J,Maga JA,Cermakian N,Cedergren R,Feagin JE

    更新日期:2001-04-06 00:00:00

  • Expression and cellular localization of Trypanosoma cruzi type II DNA topoisomerase.

    abstract::Topoisomerases are enzymes that participate in many cellular functions involving topological manipulation of DNA strands. There are two types of topoisomerases in the cell: (a) type I topoisomerases; and (b) type II topoisomerases (topo II). Previously we have cloned and sequenced the gene encoding Trypanosoma cruzi t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fragoso SP,Mattei D,Hines JC,Ray D,Goldenberg S

    更新日期:1998-08-01 00:00:00

  • Genomic organization, chromosomal location and transcription of dispersed and repeated tubulin genes in Leishmania major.

    abstract::The alpha and beta tubulin genes of Leishmania major were cloned and used to study the genomic organization, chromosomal location and transcription of tubulin genes in L. major. The number of beta tubulin isogenes was determined by hybridization of probes representing the 5' and 3' ends of the cloned beta tubulin cDNA...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Spithill TW,Samaras N

    更新日期:1987-05-01 00:00:00

  • Molecular cloning and characterization of the polypeptide backbone of Schistosoma mansoni circulating cathodic antigen.

    abstract::One of the candidate schistosome antigens for the development of a circulating antigen detection diagnostic assay is the circulating cathodic antigen (CCA). Detection of CCA in urine provides a non-invasive assay with high sensitivity. Previously we reported that CCA is secreted in patients' urine as a small molecular...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Abdeen HH,Attallah AF,Mansour MM,Harrison RA

    更新日期:1999-06-25 00:00:00

  • Alpha and beta subunits of pyruvate dehydrogenase E1 from the microsporidian Nosema locustae: mitochondrion-derived carbon metabolism in microsporidia.

    abstract::Microsporidia are highly adapted eukaryotic intracellular parasites that infect a variety of animals. Microsporidia contain no recognisable mitochondrion, but recently have been shown to have evolved from fungi and to possess heat shock protein genes derived from mitochondria. These findings make it clear that microsp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fast NM,Keeling PJ

    更新日期:2001-10-01 00:00:00

  • Membrane modifications in erythrocytes parasitized by Plasmodium falciparum.

    abstract::Plasmodium falciparum malaria parasites invade human red blood cells and immediately begin making significant alterations to the structure of the erythrocyte. These alterations facilitate the movement of nutrients into, and waste products and parasite-derived proteins out of the cell to meet the needs of the growing p...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Deitsch KW,Wellems TE

    更新日期:1996-02-01 00:00:00

  • L-Glutamine uptake is developmentally regulated and is involved in metacyclogenesis in Trypanosoma cruzi.

    abstract::Trypanosoma cruzi, the aetiological agent of Chagas disease, can obtain L-glutamine (Gln) through the enzyme glutamine synthetase (GS) using glutamate (Glu) and ammonia as substrates. In this work, we show additional non-canonical roles for this amino acid: its involvement in ATP maintenance and parasite survival unde...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Damasceno FS,Barisón MJ,Crispim M,Souza ROO,Marchese L,Silber AM

    更新日期:2018-09-01 00:00:00

  • Genetic variability and evolution of the Schistosoma genome analysed by using random amplified polymorphic DNA markers.

    abstract::The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barral V,This P,Imbert-Establet D,Combes C,Delseny M

    更新日期:1993-06-01 00:00:00

  • Identification and expression analysis of ABC protein-encoding genes in Toxoplasma gondii. Toxoplasma gondii ATP-binding cassette superfamily.

    abstract::The ATP-binding cassette (ABC) transporters are one of the largest evolutionarily conserved families of proteins. They are characterized by the presence of nucleotide-binding domains (NBDs), which are highly conserved among organisms. In the present study, we used human and protozoan ABC sequences, and ATP-binding con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sauvage V,Millot JM,Aubert D,Visneux V,Marle-Plistat M,Pinon JM,Villena I

    更新日期:2006-06-01 00:00:00

  • Recent independent evolution of msp1 polymorphism in Plasmodium vivax and related simian malaria parasites.

    abstract::The Plasmodium MSP-1 is a promising malaria vaccine candidate. However, the highly polymorphic nature of the MSP-1 gene (msp1) presents a potential obstacle for effective vaccine development. To investigate the evolutionary history of msp1 polymorphism in P. vivax, we construct phylogenetic trees of msp1 from P. vivax...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tanabe K,Escalante A,Sakihama N,Honda M,Arisue N,Horii T,Culleton R,Hayakawa T,Hashimoto T,Longacre S,Pathirana S,Handunnetti S,Kishino H

    更新日期:2007-11-01 00:00:00

  • The subcellular localisation of trypanosome RRP6 and its association with the exosome.

    abstract::The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Haile S,Cristodero M,Clayton C,Estévez AM

    更新日期:2007-01-01 00:00:00

  • Sequence preference of chloroquine binding to DNA and prevention of Z-DNA formation.

    abstract::Chloroquine is a critically important antimalarial drug and a well known intercalator into DNA. We now show that chloroquine binds more avidly to poly(dG-dC).poly(dG-dC) than to other synthetic polynucleotides and that this binding inhibits both salt- and cobalt-induced transitions to Z-DNA. These data are consistent ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kwakye-Berko F,Meshnick S

    更新日期:1990-03-01 00:00:00

  • A phylogenetic analysis of the Trypanosoma cruzi genome project CL Brener reference strain by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting.

    abstract::We have assessed the phylogenetic status of the Trypanosoma cruzi Genome Project CL Brener reference strain by multilocus enzyme electrophoresis (MLEE) and multiprimer random amplified polymorphic DNA (RAPD) including a set of cloned stocks representative of the whole genetic diversity of T. cruzi. MLEE and RAPD data ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Brisse S,Barnabé C,Bañuls AL,Sidibé I,Noël S,Tibayrenc M

    更新日期:1998-05-01 00:00:00

  • The secreted and somatic antigens of the third stage larva of Anisakis simplex, and antigenic relationship with Ascaris suum, Ascaris lumbricoides, and Toxocara canis.

    abstract::The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

    更新日期:1988-10-01 00:00:00