New tools to identify var sequence tags and clone full-length genes using type-specific primers to Duffy binding-like domains.


:Cytoadherence of infected erythrocytes is a hallmark of Plasmodium falciparum infection and a key determinant in the particular virulence of this species. Infected erythrocytes bind a variety of host receptors but certain adhesion traits are associated with more severe disease. A large, diverse protein family named P. falciparum erythrocyte membrane protein 1 (PfEMP1) is responsible for sequestration of mature stage infected erythrocytes and orchestrates parasite binding tropism. To better understand the molecular basis for malaria disease, more study is needed to identify the subset of PfEMP1 variants that contribute to basic disease phenotypes. PfEMP1 proteins have multiple receptor-like domains that group into different homology types based upon sequence similarity. Universal primers have been developed that recognize some, but not all PfEMP1 adhesion domain types. In this study, we designed and validated a new series of type-discriminatory primers to the DBL-beta, -gamma, and -delta adhesion types for epidemiological profiling. In addition, we used new primers to the var upstream region and exon 2 to demonstrate how the strategic placement of primers throughout the gene structure can be exploited to efficiently clone the var gene coding region. These new approaches provide valuable tools to gain novel insights into cytoadherence and malaria pathogenesis.


Mol Biochem Parasitol


Kraemer SM,Gupta L,Smith JD




Has Abstract


2003-06-01 00:00:00














  • Nucleotide binding properties of a P-glycoprotein homologue from Plasmodium falciparum.

    abstract::The Plasmodium falciparum P-glycoprotein homologue 1 (PGH1) is structurally similar to several members of the ATP-binding cassette (ABC) superfamily of membrane transporters. We have examined whether the nucleotide binding domains predicted from the deduced amino sequence are functional by photoaffinity labeling of pu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Karcz SR,Galatis D,Cowman AF

    更新日期:1993-04-01 00:00:00

  • Characterisation of the novel junctions of two minichromosomes of Leishmania major.

    abstract::The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ortiz G,Segovia M

    更新日期:1996-11-25 00:00:00

  • An evaluation of different methods for labelling the surface of the filarial nematode Brugia pahangi with 125iodine.

    abstract::The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marshall E,Howells RE

    更新日期:1985-06-01 00:00:00

  • Choline transport in Leishmania major promastigotes and its inhibition by choline and phosphocholine analogs.

    abstract::Phosphatidylcholine is the most abundant phospholipid in the membranes of the human parasite Leishmania. The metabolic pathways leading to its biosynthesis are likely to play a critical role in parasite development and survival and may offer a good target for antileishmanial chemotherapy. Phosphatidylcholine synthesis...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zufferey R,Mamoun CB

    更新日期:2002-11-01 00:00:00

  • In situ analysis of a variant surface glycoprotein expression-site promoter region in Trypanosoma brucei.

    abstract::In Trypanosoma brucei, the active variant surface glycoprotein genes (vsg) are located at telomeric expression sites (ES), whose expression is highly regulated during the life cycle. In the procyclic form, all ESs are repressed. In the bloodstream form, where antigenic variation occurs, only one of approximately 20 ES...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Navarro M,Cross GA

    更新日期:1998-07-01 00:00:00

  • A Plasmodium falciparum isolate with a chromosome 9 deletion expresses a trypsin-resistant cytoadherence molecule.

    abstract::Sequestration of Plasmodium falciparum infected erythrocytes in the cerebral circulation is strongly implicated in the pathogenesis of cerebral malaria. From previous studies it was postulated that genes essential for cytoadherence were located on the right arm of chromosome 9 as P. falciparum isolates with a deletion...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chaiyaroj SC,Coppel RL,Magowan C,Brown GV

    更新日期:1994-09-01 00:00:00

  • Identification of the Echinococcus (hydatid disease) organisms using cloned DNA markers.

    abstract::Cloned DNA fragments of the ribosomal RNA gene of Schistosoma mansoni hybridise strongly to Echinococcus DNA following restriction endonuclease and Southern transfer analysis. Individuals within a strain of E. granulosus exhibit identical patterns of hybridisation. However, the hybridisation patterns show significant ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: McManus DP,Simpson AJ

    更新日期:1985-11-01 00:00:00

  • Identification of Toxoplasma TgPH1, a pleckstrin homology domain-containing protein that binds to the phosphoinositide PI(3,5)P2.

    abstract::The phosphoinositide phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) plays crucial roles in the maintenance of lysosome/vacuole morphology, membrane trafficking and regulation of endolysosome-localized membrane channel activity. In Toxoplasma gondii, we previously reported that PI(3,5)P2 is essential for parasite su...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Daher W,Morlon-Guyot J,Alayi TD,Tomavo S,Wengelnik K,Lebrun M

    更新日期:2016-05-01 00:00:00

  • Characterization of a Fasciola gigantica protein carrying two DM9 domains reveals cellular relocalization property.

    abstract::Even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. Classified among the proteins of unknown function is a family of proteins harboring repeats of the DM9 domain, a 60-75 amino acids motif first described in a sma...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Phadungsil W,Smooker PM,Vichasri-Grams S,Grams R

    更新日期:2016-01-01 00:00:00

  • Anopheles gambiae laminin interacts with the P25 surface protein of Plasmodium berghei ookinetes.

    abstract::Laminin is a major constituent of the basal lamina surrounding the midgut of the malaria vectors that has been implicated in the development of the Plasmodium oocyst. In this report we describe the cloning of the Anopheles gambiae gene encoding the laminin gamma 1 polypeptide and follow its expression during mosquito ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Vlachou D,Lycett G,Sidén-Kiamos I,Blass C,Sinden RE,Louis C

    更新日期:2001-02-01 00:00:00

  • Peroxisomal targeting protein 14 (PEX14) from Leishmania donovani. Molecular, biochemical, and immunocytochemical characterization.

    abstract::Pathogens of the Leishmania and Trypanosoma genera compartmentalize glycolytic and other nutritional pathways in glycosomes, unique subcellular organelles related to the peroxisomes of mammals and yeasts. Most glycosomal proteins are targeted to the glycosomes by a COOH-terminal tripeptide signal similar to the peroxi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jardim A,Rager N,Liu W,Ullman B

    更新日期:2002-09-01 00:00:00

  • The variable region of the Trypanosoma brucei kinetoplast maxicircle: sequence and transcript analysis of a repetitive and a non-repetitive fragment.

    abstract::The sequence of two fragments derived from the variable region of the kinetoplast maxicircle of Trypanosoma brucei has been determined. One fragment (1334 nucleotides, situated immediately upstream of the 12S and 9S ribosomal RNA genes) consists of non-repetitious DNA, which does not hybridize to other maxicircle regi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: de Vries BF,Mulder E,Brakenhoff JP,Sloof P,Benne R

    更新日期:1988-01-01 00:00:00

  • A novel rhoptry protein in Toxoplasma gondii bradyzoites and merozoites.

    abstract::The secretory organelles of Toxoplasma gondii orchestrate invasion of the host cell and establish the parasitophorous vacuole. Although much has been learned about the roles played by these organelles in invasion by the tachyzoite stage, little is known about the contents or functions of these organelles during bradyz...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schwarz JA,Fouts AE,Cummings CA,Ferguson DJ,Boothroyd JC

    更新日期:2005-12-01 00:00:00

  • Repeat structures in a Plasmodium falciparum protein (MESA) that binds human erythrocyte protein 4.1.

    abstract::The mature-parasite-infected erythrocyte surface antigen (MESA, also known as PfEMP-2 and pp300) of Plasmodium falciparum is a phosphoprotein of approx. 250-300 kDa that is exported from the parasite to the erythrocyte membrane skeleton where it binds to protein 4.1. Determination of the primary sequence of MESA revea...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Coppel RL

    更新日期:1992-02-01 00:00:00

  • Base J glucosyltransferase does not regulate the sequence specificity of J synthesis in trypanosomatid telomeric DNA.

    abstract::Telomeric DNA of trypanosomatids possesses a modified thymine base, called base J, that is synthesized in a two-step process; the base is hydroxylated by a thymidine hydroxylase forming hydroxymethyluracil (hmU) and a glucose moiety is then attached by the J-associated glucosyltransferase (JGT). To examine the importa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bullard W,Cliffe L,Wang P,Wang Y,Sabatini R

    更新日期:2015-12-01 00:00:00

  • Antagonism of sulfadoxine and pyrimethamine antimalarial activity in vitro by p-aminobenzoic acid, p-aminobenzoylglutamic acid and folic acid.

    abstract::The activity of pyrimethamine and sulfadoxine against two strains of Plasmodium falciparum has been studied in vitro by a radioisotopic technique. Low level antagonism of pyrimethamine resulted from the inclusion of p-aminobenzoic acid, p-aminobenzoylglutamic acid or folic acid in the test medium. Sulfadoxine activity...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Watkins WM,Sixsmith DG,Chulay JD,Spencer HC

    更新日期:1985-01-01 00:00:00

  • A role for tertiary structure in the generation of antigenic diversity and molecular association of the Tams1 polypeptide in Theileria annulata.

    abstract::The major merozoite-piroplasm surface antigen (mMPSA) of Theileria annulata, Tams1, is known to be antigenically diverse. The possession of variable N-linked glycosylation sites and removal of monoclonal antibody 5E1 reactivity by mild periodate treatment suggested, previously, that divergent epitopes may be conferred...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Katzer F,McKellar S,Ferguson MA,d'Oliveira C,Shiels BR

    更新日期:2002-06-01 00:00:00

  • Heterogeneity and expression of the Plasmodium falciparum 5.8S ribosomal RNA genes.

    abstract::The number and expression of some of the large ribosomal RNA (rRNA) gene classes present in the human malaria parasite Plasmodium falciparum was determined. Southern blot analyses, using the 5.8S rRNA coding region as a marker, indicate that the P. falciparum genome contains at least 5 distinct subclasses of large rRN...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Shippen-Lentz D,Afroze T,Vezza AC

    更新日期:1990-01-01 00:00:00

  • Homologous telomeric sequences are present in different species of the genus Plasmodium.

    abstract::The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dore E,Pace T,Ponzi M,Scotti R,Frontali C

    更新日期:1986-11-01 00:00:00

  • Molecular cloning and analysis of Ancylostoma ceylanicum glutamate-cysteine ligase.

    abstract::Glutamate-cysteine ligase (GCL) is a heterodimer enzyme composed of a catalytic subunit (GCLC) and a modifier subunit (GCLM). This enzyme catalyses the synthesis of γ-glutamylcysteine, a precursor of glutathione. cDNAs of the putative glutamate-cysteine ligase catalytic (Ace-GCLC) and modifier subunits (Ace-GCLM) of A...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wiśniewski M,Lapiński M,Zdziarska A,Długosz E,Bąska P

    更新日期:2014-08-01 00:00:00

  • Isolation and characterization of infective and non-infective clones of Leishmania tropica.

    abstract::Cloning by limit dilution of an isolate of Leishmania tropica (LRC-L137) that is infective for mice resulted in 7 stable clones, only one of which was infective in BALB/c mice. Three of the non-infective clones that were examined for survival in BALB/c macrophages in vitro seemed to be killed more readily, suggesting ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Hocking RE,Mitchell GF,Spithill TW

    更新日期:1983-02-01 00:00:00

  • Expression differential of microsomal and cytosolic glutathione-S-transferases in Fasciola hepatica resistant at triclabendazole.

    abstract::In the present work, we evaluate in vitro the cytosolic and microsomal activity of glutathione-S-transferases in adults of Fasciola hepatica susceptible (Cullompton Strain) and resistant (Sligo Strain) to triclabendazole. The triclabendazole resistant (Sligo) fluke expressed significant major metabolic activity of glu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Scarcella S,Lamenza P,Virkel G,Solana H

    更新日期:2012-01-01 00:00:00

  • Characterisation of a simple, highly repetitive DNA sequence from the parasite Leishmania donovani.

    abstract::Repetitive DNA sequences of the Leishmania donovani genome have been identified by screening a recombinant DNA library made by cloning sheared genomic DNA into the vector pAT153. Bacterial clones containing a highly repetitive DNA sequence have been isolated. DNA sequencing has shown that this sequence is composed of ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ellis J,Crampton J

    更新日期:1988-05-01 00:00:00

  • Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi.

    abstract::The technique of RNA interference (RNAi) is exceedingly useful for knocking down the expression of a specific mRNA in African trypanosomes and other organisms for the purpose of examining the function of its gene. However, when we attempted to apply RNAi in the Latin American trypanosome, Trypanosoma cruzi, to diminis...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: DaRocha WD,Otsu K,Teixeira SM,Donelson JE

    更新日期:2004-02-01 00:00:00

  • A bioinformatic analysis of the RAB genes of Trypanosoma brucei.

    abstract::RAB proteins are small GTPases with vital roles in eukaryotic intracellular transport; orthologous RABs appear to fulfil similar functions in diverse organisms. Trypanosoma brucei spp., the causative organisms of Old World trypanosomiasis of humans and domestic animals, have extremely effective endocytic and exocytic ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ackers JP,Dhir V,Field MC

    更新日期:2005-05-01 00:00:00

  • Gender biased differential alternative splicing patterns of the transcriptional cofactor CA150 gene in Schistosoma mansoni.

    abstract::The complex molecular systems involved in the process of sex-differentiation and fertility in Schistosoma mansoni have not yet been completely described. Using a 4608-element cDNA microarray, we have now determined 90 and 139 genes with significantly (q-value

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: DeMarco R,Oliveira KC,Venancio TM,Verjovski-Almeida S

    更新日期:2006-12-01 00:00:00

  • Isolation of putative cysteine protease genes of Ostertagia ostertagi.

    abstract::Recombinant phage containing putative Ostertagia ostertagi cysteine protease genes have been isolated from a lambda EMBL-3:genomic DNA library using a Haemonchus contortus cathepsin B-like cysteine protease cDNA as hybridization probe. Restriction enzyme maps of the phages suggest that they identify at least 3 genes, ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pratt D,Boisvenue RJ,Cox GN

    更新日期:1992-11-01 00:00:00

  • Thiol groups on the surface of anaerobic parasitic protozoa.

    abstract::Evidence is presented that Giardia lamblia and Entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. Both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gillin FD,Reiner DS,Levy RB,Henkart PA

    更新日期:1984-09-01 00:00:00

  • Mechanisms of respiration and phosphorylation in Ascaris muscle mitochondria.

    abstract::In Ascaris muscle mitochondria the major respiratory chain-linked phosphorylation activity is accomplished by a NADH-linked reduction of fumarate to succinate. Oxygen can also be employed as a terminal electron acceptor via a cyanide- and salicyl-hydroxamate-resistant terminal oxidase. As in fumarate-dependent electro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Köhler P,Bachmann R

    更新日期:1980-04-01 00:00:00

  • Biochemical characterization of Toxoplasma gondii 1-Cys peroxiredoxin 2 with mechanistic similarities to typical 2-Cys Prx.

    abstract::TgPrx2 represents a recently discovered cytosolic 1-Cys peroxiredoxin (Prx) from the intracellular parasite Toxoplasma gondii. Over-expression of the respective gene confers protection against H(2)O(2), suggesting that the protein possesses peroxidase activity. According to the current nomenclature eukaryotic typical ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Deponte M,Becker K

    更新日期:2005-03-01 00:00:00