The Kennedy phospholipid biosynthesis pathways are refractory to genetic disruption in Plasmodium berghei and therefore appear essential in blood stages.

abstract：:Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

journal_title：Molecular and biochemical parasitology

authors： Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

更新日期：2012-03-01 00:00:00

abstract：:The cysteine proteinase B of Leishmania parasites is an important virulence factor. In this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative agent of mucocutaneous leishmaniosis. The mature region of the recombinant CPB shares a high p...

journal_title：Molecular and biochemical parasitology

authors： Lanfranco MF,Loayza-Muro R,Clark D,Núñez R,Zavaleta AI,Jimenez M,Meldal M,Coombs GH,Mottram JC,Izidoro M,Juliano MA,Juliano L,Arévalo J

更新日期：2008-10-01 00:00:00

abstract：:Helminths are masterful immunoregulators. A characteristic feature of helminth infection is a Th2-dominated immune response, but stimulation of immunoregulatory cell populations, such as regulatory T cells and alternatively activated macrophages, is equally common. Typically, Th1/17 immunity is blocked and productive ...

journal_title：Molecular and biochemical parasitology

authors： Hewitson JP,Grainger JR,Maizels RM

更新日期：2009-09-01 00:00:00

abstract：:A gene encoding the small subunit rRNA (SSUrRNA) has been isolated from the human parasite, Plasmodium malariae. The gene has been sequenced. It contains conserved and variable regions which conform to patterns established for other eukaryotic SSUrRNA genes. Comparisons with other SSUrRNA genes from Plasmodium species...

journal_title：Molecular and biochemical parasitology

authors： Goman M,Mons B,Scaife J

更新日期：1991-04-01 00:00:00

abstract：:Surface labeling studies using two populations of Onchocerca gibsoni microfilariae revealed important differences in major radioiodinated proteins. Small numbers of microfilariae harvested from the skin of cattle or the uteri of adult worms from skin nodules were purified, radioiodinated, solubilized and the proteins ...

journal_title：Molecular and biochemical parasitology

authors： Forsyth KP,Copeman DB,Mitchell GF

更新日期：1984-02-01 00:00:00

abstract：:Three species of blood fluke from the genus Cardicola are known to parasitize and cause disease in Bluefin Tunas--C. forsteri, C. orientalis, and C. opisthorchis. Although initially believed to be separated by geography and host specificity, recent identification of at least two Cardicola spp. concurrently present wit...

journal_title：Molecular and biochemical parasitology

authors： Polinski M,Hamilton DB,Nowak B,Bridle A

更新日期：2013-09-01 00:00:00

abstract：:A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secr...

journal_title：Molecular and biochemical parasitology

authors： Hines SA,Palmer GH,Brown WC,McElwain TF,Suarez CE,Vidotto O,Rice-Ficht AC

更新日期：1995-02-01 00:00:00

abstract：:Plasmodium falciparum extensively modifies the infected red blood cell (RBC), resulting in changes in deformability, shape and surface properties. These alterations suggest that the RBC cytoskeleton is a major target for modification during infection. However, the molecular mechanisms leading to these changes are larg...

journal_title：Molecular and biochemical parasitology

authors： Shakya B,Penn WD,Nakayasu ES,LaCount DJ

更新日期：2017-09-01 00:00:00

abstract：:The pyrimidine biosynthesis pathway in the protozoan pathogen Toxoplasma gondii is essential for parasite growth during infection. To investigate the properties of dihydroorotate dehydrogenase (TgDHOD), the fourth enzyme in the T. gondii pyrimidine pathway, we expressed and purified recombinant TgDHOD. TgDHOD exhibite...

journal_title：Molecular and biochemical parasitology

authors： Hortua Triana MA,Huynh MH,Garavito MF,Fox BA,Bzik DJ,Carruthers VB,Löffler M,Zimmermann BH

更新日期：2012-08-01 00:00:00

abstract：:Two different classes of small nematode specific lipid-binding proteins, the nematode polyprotein allergens/antigens (NPAs) and the fatty acid- and retinol-binding (FAR) proteins, are secreted by helminth parasites. Until now, there was no evidence of the expression or secretion of these two families of proteins in Ha...

journal_title：Molecular and biochemical parasitology

authors： Kuang L,Colgrave ML,Bagnall NH,Knox MR,Qian M,Wijffels G

更新日期：2009-11-01 00:00:00

abstract：:Proteins in malaria parasites (Plasmodium falciparum) isolated from a patient in Thailand before treatment, and after recrudescence of infection subsequent to mefloquine treatment, were compared by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis. Nine 'pre-treatment' and six 'recrudescent' clones...

journal_title：Molecular and biochemical parasitology

authors： Pinswasdi C,Thaithong S,Beale GH,Fenton B,Webster HK,Pavanand K

更新日期：1987-03-01 00:00:00

abstract：:Antibodies were affinity purified from crude antiserum by elution from the 24 kDa region of preparative one-dimensional Western blots containing immobilized adult Schistosoma mansoni inner bilayer membrane proteins. They were shown to be specific for a single acidic polypeptide complex, Smgp24, following immunoblottin...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Barnard BJ,Podesta RB

更新日期：1988-11-01 00:00:00

abstract：:Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

journal_title：Molecular and biochemical parasitology

authors： Gao F,Voncken F,Colasante C

更新日期：2020-05-01 00:00:00

abstract：:Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In th...

journal_title：Molecular and biochemical parasitology

authors： Nava S,Sadiqova A,Castellanos-Gonzalez A,White AC Jr

更新日期：2020-05-01 00:00:00

abstract：:We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(...

journal_title：Molecular and biochemical parasitology

authors： Gilchrist CA,Streets HL,Ackers JP,Hall R

更新日期：1995-10-01 00:00:00

abstract：:Antigenic diversity was observed in the circumsporozoite (CS) proteins of five of the six Plasmodium cynomolgi isolates (NIH, Mulligan, London, Gombak, Ceylon, RO) that we examined. Monoclonal antibodies were produced against salivary gland sporozoites of three of the isolates. Interaction of these monoclonal antibodi...

journal_title：Molecular and biochemical parasitology

authors： Cochrane AH,Gwadz RW,Ojo-Amaize E,Hii J,Nussenzweig V,Nussenzweig RS

更新日期：1985-01-01 00:00:00

abstract：:Cyclic AMP-dependent protein kinase (protein kinase A, PKA) is a key element in many cell signaling pathways. An essential role of Plasmodium falciparum PKA (PfPKA) activity was reported in the intraerythrocytic growth of the malaria parasite. However, molecular characterization of PfPKA using purified recombinant pro...

journal_title：Molecular and biochemical parasitology

authors： Sudo A,Kato K,Kobayashi K,Tohya Y,Akashi H

更新日期：2008-08-01 00:00:00

abstract：:The nucleotide sequence of the cloned Echinococcus multilocularis DNA probe pAL1 was determined in order to simplify and improve the sensitivity of a diagnostic assay through the application of the polymerase chain reaction (PCR). The insert-specific oligonucleotides BG1 and BG2 define a 2.6-kb fragment in the genomic...

journal_title：Molecular and biochemical parasitology

authors： Gottstein B,Mowatt MR

更新日期：1991-02-01 00:00:00

abstract：:Giardia lamblia is an early diverging eukaryote which causes gastrointestinal disease throughout the world. Different subgroups of Giardia have been defined based on several biochemical and genetic criteria. We have developed a method for stably introducing DNA into the nuclei of the parasite using puromycin acetyltra...

journal_title：Molecular and biochemical parasitology

authors： Singer SM,Yee J,Nash TE

更新日期：1998-04-01 00:00:00

abstract：:Trypanosoma cruzi, the aetiological agent of Chagas disease, can obtain L-glutamine (Gln) through the enzyme glutamine synthetase (GS) using glutamate (Glu) and ammonia as substrates. In this work, we show additional non-canonical roles for this amino acid: its involvement in ATP maintenance and parasite survival unde...

journal_title：Molecular and biochemical parasitology

authors： Damasceno FS,Barisón MJ,Crispim M,Souza ROO,Marchese L,Silber AM

更新日期：2018-09-01 00:00:00

abstract：:Chloroquine is a critically important antimalarial drug and a well known intercalator into DNA. We now show that chloroquine binds more avidly to poly(dG-dC).poly(dG-dC) than to other synthetic polynucleotides and that this binding inhibits both salt- and cobalt-induced transitions to Z-DNA. These data are consistent ...

journal_title：Molecular and biochemical parasitology

authors： Kwakye-Berko F,Meshnick S

更新日期：1990-03-01 00:00:00

abstract：:The electrophoretic patterns of hexokinase and phosphoglucomutase have been widely used to distinguish Entamoeba histolytica from Entamoeba dispar isolates. Although E. histolytica and E. dispar, previously called pathogenic and nonpathogenic Entamoeba histolytica, differ clearly in sequences of many homologous genes,...

journal_title：Molecular and biochemical parasitology

authors： Ortner S,Clark CG,Binder M,Scheiner O,Wiedermann G,Duchêne M

更新日期：1997-05-01 00:00:00

abstract：:The mature-parasite-infected erythrocyte surface antigen (MESA, also known as PfEMP-2 and pp300) of Plasmodium falciparum is a phosphoprotein of approx. 250-300 kDa that is exported from the parasite to the erythrocyte membrane skeleton where it binds to protein 4.1. Determination of the primary sequence of MESA revea...

journal_title：Molecular and biochemical parasitology

authors： Coppel RL

更新日期：1992-02-01 00:00:00

abstract：:Repeated DNA sequences have been instrumental in the development of DNA probes for many different parasites. Isolation of such DNA probes has generally been accomplished by differential screening of genomic libraries with total genomic DNA preparations. In the current work, a rational design strategy is presented for ...

journal_title：Molecular and biochemical parasitology

authors： Zimmerman PA,Toe L,Unnasch TR

更新日期：1993-04-01 00:00:00

abstract：:The livers of chickens maintained on a diet containing an effective in vivo level (70 ppm) of arprinocid, an anticoccidial agent, were found to contain 0.64 ppm of this drug and 0.33 ppm of a metabolite, arprinocid-1-N-oxide. When arprinocid was tested against Eimeria tenella in cultures of chick kidney epithelial cel...

journal_title：Molecular and biochemical parasitology

authors： Wang CC,Simashkevich PM

更新日期：1980-10-01 00:00:00

abstract：:A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

journal_title：Molecular and biochemical parasitology

authors： el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

更新日期：1995-07-01 00:00:00

abstract：:The promoter region of the ribosomal RNA (rRNA) genes of Leishmania amazonensis was characterised and the transcription start point, defined by primer extension, was shown to be a T residue, 1048 nucleotides upstream of the beginning of the 18S sequence. A repetitive element of 60 bp was identified in the intergenic s...

journal_title：Molecular and biochemical parasitology

authors： Uliana SR,Fischer W,Stempliuk VA,Floeter-Winter LM

更新日期：1996-02-01 00:00:00

abstract：:Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes ...

journal_title：Molecular and biochemical parasitology

authors： Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM

更新日期：2020-07-01 00:00:00

abstract：:This study is the first demonstration of individual gene products synthesized by Hymenolepis diminuta. To identify proteins synthesized specifically by H. diminuta, we radiolabeled adult worms with [14C]leucine in vitro. Subsequent one- and two-dimensional gel electrophoretic separation and staining of the protein con...

journal_title：Molecular and biochemical parasitology

authors： Atkinson BG,Podesta RB

更新日期：1982-07-01 00:00:00

abstract：:Giardia lamblia undergoes surface antigenic variation. The variant-specific surface proteins (VSPs) of isolate WB are cysteine-rich, can vary dramatically in size, contain Cys-X-X-Cys motifs, and are differentially expressed. GS/M(H7) is a Giardia clone from a different isolate which expresses a VSP epitope not found ...

journal_title：Molecular and biochemical parasitology

authors： Nash TE,Mowatt MR

更新日期：1992-04-01 00:00:00