Abstract:
:Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes nucleotide exchange on G-actin and promotes actin polymerization at its low concentrations. However, at high concentrations, it strongly inhibits the polymerization process by sequestering actin monomers. We further demonstrate that LdPfn binds to actin in Leishmania promastigotes, by both immunofluorescence microscopy and IgG affinity chromatography. Further, we reveal that this protein besides binding to poly-null-proline motifs, also binds more efficiently to PI(3,5)P2, which is found on early or late endosomes or lysosomes, than to PI(4,5)P2 and PI(3,4,5)P3. Additionally, we show that heterozygous mutants of profilin display significantly slower growth and intracellular vesicle trafficking activity, which is reversed on episomal gene complementation. Together, these findings suggest that profilin regulates intracellular vesicle trafficking in Leishmania perhaps through its binding to polyphosphoinositides.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CMdoi
10.1016/j.molbiopara.2020.111280subject
Has Abstractpub_date
2020-07-01 00:00:00pages
111280eissn
0166-6851issn
1872-9428pii
S0166-6851(20)30044-Xjournal_volume
238pub_type
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journal_title:Molecular and biochemical parasitology
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