Actin sequestering protein, profilin, regulates intracellular vesicle transport in Leishmania.


:Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes nucleotide exchange on G-actin and promotes actin polymerization at its low concentrations. However, at high concentrations, it strongly inhibits the polymerization process by sequestering actin monomers. We further demonstrate that LdPfn binds to actin in Leishmania promastigotes, by both immunofluorescence microscopy and IgG affinity chromatography. Further, we reveal that this protein besides binding to poly-null-proline motifs, also binds more efficiently to PI(3,5)P2, which is found on early or late endosomes or lysosomes, than to PI(4,5)P2 and PI(3,4,5)P3. Additionally, we show that heterozygous mutants of profilin display significantly slower growth and intracellular vesicle trafficking activity, which is reversed on episomal gene complementation. Together, these findings suggest that profilin regulates intracellular vesicle trafficking in Leishmania perhaps through its binding to polyphosphoinositides.


Mol Biochem Parasitol


Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM




Has Abstract


2020-07-01 00:00:00












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    journal_title:Molecular and biochemical parasitology

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    authors: Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

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    abstract::We have shown that the Leishmania major transfection vector pR-NEO (or derivatives thereof) can be introduced and stably maintained in four species complexes of pathogenic Leishmania (L. tropica, L. mexicana, L. donovani, L. braziliensis), and the genera Endotrypanum and Crithidia; transfection of Trypanosoma cruzi or...

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    authors: Coburn CM,Otteman KM,McNeely T,Turco SJ,Beverley SM

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    pub_type: 杂志文章,评审


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    authors: Obregón-Henao A,Gil DL,Gómez DI,Sanzón F,Teale JM,Restrepo BI

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    pub_type: 杂志文章


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    authors: Morales ME,Rinaldi G,Gobert GN,Kines KJ,Tort JF,Brindley PJ

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    pub_type: 杂志文章


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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hussein AS,Walter RD

    更新日期:1995-06-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ou YC,Giroud C,Baltz T

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ohnishi T,Lloyd D,Lindmark DG,Müller M

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wallach M

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bhattacharjee S,Speicher KD,Stahelin RV,Speicher DW,Haldar K

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Berens RL,Marr JJ,Brun R

    更新日期:1980-04-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Templeton TJ

    更新日期:2009-08-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fast NM,Keeling PJ

    更新日期:2001-10-01 00:00:00