Abstract：
:The glycoproteins of 12-28 kD from Taenia solium metacestodes provide a high specificity and sensitivity for the serological diagnosis of the central nervous system infection, neurocysticercosis. Their widespread use as antigens for routine serological assays will require their production in large and reproducible amounts. Prior to determining the ideal strategy to produce these antigens at a large scale, it is important to determine the contribution of the carbohydrates to the antigenicity of these molecules, given the uncertainty of reproducing saccharidic epitopes in recombinant expression systems. In this study we examined this issue. The chemical oxidation of the carbohydrates of the 12-28 kD glycoproteins with sodium metaperiodate, reduced the antigenicity of the molecules to variable extents, with the more notable changes being detected for the 18 and 28 kD antigens. This approach was complemented by purification of the 12, 16 and 18 kD antigens, followed by the enzymatic deglycosylation of their abundant N-linked oligosaccharides. Silver-stained SDS-PAGE analysis indicated that the three deglycosylated antigens now migrated as 7 kD products, suggesting a protein backbone with a similar size, but different extents of glycosylation. By Western blot, the antigenicity of these antigens was diminished. This was more notable for the 18 kD antigen, which is more heavily glycosylated than the 12 or 16 kD glycoproteins. These data suggest that the antigenicity of the glycoproteins of T. solium is due to a combination of carbohydrate and protein epitopes.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Obregón-Henao A,Gil DL,Gómez DI,Sanzón F,Teale JM,Restrepo BIdoi
10.1016/s0166-6851(01)00256-0subject
Has Abstractpub_date
2001-05-01 00:00:00pages
209-15issue
2eissn
0166-6851issn
1872-9428pii
S0166685101002560journal_volume
114pub_type
杂志文章abstract：:Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...
journal_title：Molecular and biochemical parasitology
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2020-05-01 00:00:00
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2006-06-01 00:00:00
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更新日期：2006-11-01 00:00:00
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章,评审
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更新日期：2014-07-01 00:00:00
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
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