Pyrazolopyrimidine metabolism in African trypanosomes: metabolic similarities to Trypanosoma cruzi and Leishmania spp.

abstract：:Human hookworms (Ancylostoma duodenale, Necator americanus) are a major cause of malnutrition and anemia, particularly in children, and high worm burdens can lead to stunted growth and mental retardation. Mass drug administration (MDA) with benzimidazole (BZ) anthelmintics has the potential to greatly reduce morbidity...

journal_title：Molecular and biochemical parasitology

authors： Schwenkenbecher JM,Albonico M,Bickle Q,Kaplan RM

更新日期：2007-12-01 00:00:00

abstract：:Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

journal_title：Molecular and biochemical parasitology

authors： Gao F,Voncken F,Colasante C

更新日期：2020-05-01 00:00:00

abstract：:The promastigote surface antigen 2 (PSA-2) complex comprises a family of antigenically similar polypeptides of M(r) 96,000, 80,000 and 50,000, anchored to the membrane with glycosylphosphatidylinositol. Although PSA-2 was initially detected only in promastigotes, Northern blot analysis indicated that mRNA transcripts ...

journal_title：Molecular and biochemical parasitology

authors： Handman E,Osborn AH,Symons F,van Driel R,Cappai R

更新日期：1995-11-01 00:00:00

abstract：:The human malarial parasite Plasmodium falciparum secretes a histidine-rich protein (HRP-II) from infected erythrocytes. HRP-II has a very high content of histidine (H) (34%), alanine (A) (37%) and aspartic acid (D) (10%) and many contiguous repeats of the sequences AHH and AHHAAD. The histidine content of the protein...

journal_title：Molecular and biochemical parasitology

authors： Panton LJ,McPhie P,Maloy WL,Wellems TE,Taylor DW,Howard RJ

更新日期：1989-06-15 00:00:00

abstract：:Antisera from lambs immunised with the Haemonchus contortus integral membrane protein complex, Haemonchus galactose-containing glycoprotein (H-gal-GP), the lambs being refractory to subsequent challenge, were used to identify several clones from an adult H. contortus lambda gt11 cDNA library. Using gene-specific oligo...

journal_title：Molecular and biochemical parasitology

authors： Redmond DL,Knox DP,Newlands G,Smith WD

更新日期：1997-03-01 00:00:00

abstract：:The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

journal_title：Molecular and biochemical parasitology

authors： Cunha VM,Meyer-Fernandes JR,Noël F

更新日期：1992-06-01 00:00:00

abstract：:Homogenates of Trypanoplasma borelli were subjected to subcellular fractionation by sequential differential and isopycnic centrifugation in sucrose. Glycerol-3-phosphate dehydrogenase and the glycolytic enzymes, glucosephosphate isomerase and triosephosphate isomerase, as well as the peroxisomal marker enzyme catalase...

journal_title：Molecular and biochemical parasitology

authors： Opperdoes FR,Nohynkova E,Van Schaftingen E,Lambeir AM,Veenhuis M,Van Roy J

更新日期：1988-08-01 00:00:00

abstract：:We studied the substrate specificity requirements of recombinant cysteine peptidases from Plasmodium falciparum, falcipain-2 (FP-2) and falcipain-3 (FP-3), using fluorescence resonance energy transfer (FRET) peptides as substrates. Systematic modifications were introduced in the lead sequence Abz-KLRSSKQ-EDDnp (Abz=or...

journal_title：Molecular and biochemical parasitology

authors： Cotrin SS,Gouvêa IE,Melo PM,Bagnaresi P,Assis DM,Araújo MS,Juliano MA,Gazarini ML,Rosenthal PJ,Juliano L,Carmona AK

更新日期：2013-02-01 00:00:00

abstract：:We have previously provided evidence for a pathway in Plasmodium falciparum-infected erythrocytes, coined the parasitophorous duct pathway, which provides serum (macro)molecules direct access to intraerythrocytic parasites . The present study addresses the purity of the fluorescent macromolecules used to define the du...

journal_title：Molecular and biochemical parasitology

authors： Goodyer ID,Pouvelle B,Schneider TG,Trelka DP,Taraschi TF

更新日期：1997-07-01 00:00:00

abstract：:Survival of Onchocerca volvulus, a pathogenic human filarial parasite, is likely to depend upon the detoxification activities of the glutathione S-transferases (GSTs). The 24 kDa O. volvulus GST, OvGST2, was expressed in a bacterial system and the recombinant protein was purified to homogeneity by affinity chromatogra...

journal_title：Molecular and biochemical parasitology

authors： Liebau E,Wildenburg G,Brophy PM,Walter RD,Henkle-Dührsen K

更新日期：1996-09-01 00:00:00

abstract：:Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid se...

journal_title：Molecular and biochemical parasitology

authors： Eakin AE,Bouvier J,Sakanari JA,Craik CS,McKerrow JH

更新日期：1990-02-01 00:00:00

abstract：:Theileria annulata is an important pathogen of cattle in the tropics. The gene sequence of a sporozoite surface antigen (SPAG-1) is reported. Data is also presented demonstrating that SPAG-1 is synthesised as a large precursor. This antigen, which is a candidate for inclusion in a subunit vaccine, shows a remarkable d...

journal_title：Molecular and biochemical parasitology

authors： Hall R,Hunt PD,Carrington M,Simmons D,Williamson S,Mecham RP,Tait A

更新日期：1992-07-01 00:00:00

abstract：:Schistosoma mansoni schistosomula cultured in the presence of phospholipids showed a decreased sensitivity to the lethal complement-mediated action of anti-schistosome antibodies. Phosphatidyl choline, sphingomyelin and phosphatidyl ethanolamine had a protective action on the schistosomula transformed in vitro by pass...

journal_title：Molecular and biochemical parasitology

authors： Billecocq A

更新日期：1987-09-01 00:00:00

abstract：:The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to...

journal_title：Molecular and biochemical parasitology

authors： Simuunza M,Bilgic H,Karagenc T,Syakalima M,Shiels B,Tait A,Weir W

更新日期：2011-06-01 00:00:00

abstract：:In trypanosomes, trans-splicing is a major essential RNA-processing mechanism that involves the addition of a spliced leader sequence to all mRNAs from a small RNA species, known as the spliced leader RNA (SL RNA). SL RNA maturation is poorly understood and it is not clear where assembly with Sm proteins takes place. ...

journal_title：Molecular and biochemical parasitology

authors： Biton M,Mandelboim M,Arvatz G,Michaeli S

更新日期：2006-12-01 00:00:00

abstract：:Laminin is a major constituent of the basal lamina surrounding the midgut of the malaria vectors that has been implicated in the development of the Plasmodium oocyst. In this report we describe the cloning of the Anopheles gambiae gene encoding the laminin gamma 1 polypeptide and follow its expression during mosquito ...

journal_title：Molecular and biochemical parasitology

authors： Vlachou D,Lycett G,Sidén-Kiamos I,Blass C,Sinden RE,Louis C

更新日期：2001-02-01 00:00:00

abstract：:This study further explores the effects of hypoxia and acute osmotic stress on intermediary metabolism of Leishmania major and Leishmania donovani. Late log phase promastigotes were washed and incubated with glucose as the sole exogenous carbon source, and rates of glucose consumption and product formation were measur...

journal_title：Molecular and biochemical parasitology

authors： Walsh MJ,Blum JJ

更新日期：1992-02-01 00:00:00

abstract：:Cytoadherence of infected erythrocytes is a hallmark of Plasmodium falciparum infection and a key determinant in the particular virulence of this species. Infected erythrocytes bind a variety of host receptors but certain adhesion traits are associated with more severe disease. A large, diverse protein family named P....

journal_title：Molecular and biochemical parasitology

authors： Kraemer SM,Gupta L,Smith JD

更新日期：2003-06-01 00:00:00

abstract：:Purified GP57/51, a Trypanosoma cruzi glycoprotein earlier identified as a major antigen in infected humans, was subjected to N-terminal sequence analysis. Alignment of the first 30 amino acids revealed that its N-terminal region is virtually identical to that reported for a cysteine-proteinase isolated from the Tulah...

journal_title：Molecular and biochemical parasitology

authors： Murta AC,Persechini PM,Padron Tde S,de Souza W,Guimarães JA,Scharfstein J

更新日期：1990-11-01 00:00:00

abstract：:Per-ARNT-Sim (PAS) domains of proteins play important roles as modules for signalling and cellular regulation processes in widely diverse organisms such as Archaea, Bacteria, protists, plants, yeasts, insects and vertebrates. These domains are present in many proteins where they are used as sensors of stimuli and modu...

journal_title：Molecular and biochemical parasitology

authors： Rojas-Pirela M,Rigden DJ,Michels PA,Cáceres AJ,Concepción JL,Quiñones W

更新日期：2018-01-01 00:00:00

abstract：:Eukaryotic cells can degrade their own components, cytosolic proteins and organelles, using dedicated hydrolases contained within the acidic interior of their lysosomes. This degradative process, called autophagy, is used under starvation conditions to recycle redundant or less important macromolecules, facilitates me...

journal_title：Molecular and biochemical parasitology

authors： Brennand A,Gualdrón-López M,Coppens I,Rigden DJ,Ginger ML,Michels PA

更新日期：2011-06-01 00:00:00

abstract：:Opisthorchis viverrini is the causative agent of human opisthorchiasis in Thailand and long lasting infection with the parasite has been correlated with the development of cholangiocarcinoma. In this work we have molecularly characterized the first member of a protein family carrying two DM9 repeats in this parasite (...

journal_title：Molecular and biochemical parasitology

authors： Labbunruang N,Phadungsil W,Tesana S,Smooker PM,Grams R

更新日期：2016-05-01 00:00:00

abstract：:A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

journal_title：Molecular and biochemical parasitology

authors： Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

更新日期：2012-09-01 00:00:00

abstract：:The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

journal_title：Molecular and biochemical parasitology

authors： Dore E,Pace T,Ponzi M,Scotti R,Frontali C

更新日期：1986-11-01 00:00:00

abstract：:Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

journal_title：Molecular and biochemical parasitology

authors： Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

更新日期：2012-03-01 00:00:00

abstract：:The influence of pH on the activity of purified pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) from Trypanosoma brucei has been studied. The Km for the coenzyme ADP is pH-dependent and shows the involvement of a dissociable group on the free enzyme with a pKa of 6.5-6.7. The cooperative interaction...

journal_title：Molecular and biochemical parasitology

authors： Flynn IW,Bowman IB

更新日期：1981-11-01 00:00:00

abstract：:Adult males and females of the dog heartworm, Dirofilaria immitis, were extracted separately and, following separation of the free and conjugated ecdysteroid fractions, the conjugates were hydrolysed enzymically. Both the ecdysteroids released by hydrolysis of the conjugates and the free hormones were further purified...

journal_title：Molecular and biochemical parasitology

authors： Mendis AH,Rose ME,Rees HH,Goodwin TW

更新日期：1983-11-01 00:00:00

abstract：:The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation ...

journal_title：Molecular and biochemical parasitology

authors： Ramos F,García G,Valadez A,Morán P,González E,Gómez A,Melendro EI,Valenzuela O,Ximénez C

更新日期：2005-06-01 00:00:00

abstract：:Glycosomes are peroxisome-like organelles present in trypanosomatid pathogens. These organelles compartmentalize glycolysis, among other reactions, and are essential in both bloodstream and procyclic form Trypanosoma brucei. Peroxins (PEXs) are proteins necessary for biogenesis of peroxisomes and glycosomes. In each a...

journal_title：Molecular and biochemical parasitology

authors： Banerjee SK,Kessler PS,Saveria T,Parsons M

更新日期：2005-07-01 00:00:00

abstract：:Antigenic variation in African trypanosomes proceeds by switching between different variant surface glycoprotein (VSG) molecules, whose extensive epitope differences enable evasion of antibody responses. Each trypanosome has approximately 1000 basic copy VSG genes inside chromosomes and a subset located at telomeres. ...

journal_title：Molecular and biochemical parasitology

authors： Graham VS,Barry JD

更新日期：1996-07-01 00:00:00