Pyrazolopyrimidine metabolism in African trypanosomes: metabolic similarities to Trypanosoma cruzi and Leishmania spp.

abstract：:In Ascaris muscle mitochondria the major respiratory chain-linked phosphorylation activity is accomplished by a NADH-linked reduction of fumarate to succinate. Oxygen can also be employed as a terminal electron acceptor via a cyanide- and salicyl-hydroxamate-resistant terminal oxidase. As in fumarate-dependent electro...

journal_title：Molecular and biochemical parasitology

authors： Köhler P,Bachmann R

更新日期：1980-04-01 00:00:00

abstract：:Leishmania major promastigotes grown in late log phase were incubated with glucose as sole exogenous carbon source in the presence of 5% CO2 and the amounts of glucose consumed and of the major products formed--succinate, pyruvate, alanine, acetate, glycerol, and D-lactate--were measured as a function of pO2. Glucose ...

journal_title：Molecular and biochemical parasitology

authors： Keegan F,Blum JJ

更新日期：1990-03-01 00:00:00

abstract：:The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

journal_title：Molecular and biochemical parasitology

authors： Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

更新日期：1988-10-01 00:00:00

abstract：:Cystic echinococcosis in humans and livestock animals is caused by infection with the cestode parasite Echinococcus granulosus. A number of genotypes of the parasite (designated G1-G10) are known to exist, with the genotype cluster G1-G3 and genotype G6 being responsible for the majority of humans infections. A recomb...

journal_title：Molecular and biochemical parasitology

authors： Alvarez Rojas CA,Gauci CG,Nolan MJ,Harandi MF,Lightowlers MW

更新日期：2012-06-01 00:00:00

abstract：:Surface labeling studies using two populations of Onchocerca gibsoni microfilariae revealed important differences in major radioiodinated proteins. Small numbers of microfilariae harvested from the skin of cattle or the uteri of adult worms from skin nodules were purified, radioiodinated, solubilized and the proteins ...

journal_title：Molecular and biochemical parasitology

authors： Forsyth KP,Copeman DB,Mitchell GF

更新日期：1984-02-01 00:00:00

abstract：:The synchronization of intraerythrocytic maturation of Plasmodium parasites is an important factor in the malaria infection process. Synchronization is mediated by inositol phosphate (InsP(x))-induced Ca(2+)-release from internal stores. To further investigate the InsP(x) metabolism in these parasites a Plasmodium pro...

journal_title：Molecular and biochemical parasitology

authors： Stritzke C,Nalaskowski MM,Fanick W,Lin H,Mayr GW

更新日期：2012-12-01 00:00:00

abstract：:P-glycoprotein gene amplification has been described in several drug-resistant parasitic protozoa. The first P-glycoprotein related gene described in Leishmania was ltpgpA, a gene frequently amplified in arsenite resistant Leishmania. Hybridization experiments indicated that ltpgpA was part of a gene family. In additi...

journal_title：Molecular and biochemical parasitology

authors： Légaré D,Hettema E,Ouellette M

更新日期：1994-11-01 00:00:00

abstract：:Two NAD(H)-dependent malate dehydrogenase (MDH) isoenzymes were detected in Phytomonas isolated from the lactiferous tubes of Euphorbia characias. The total specific activity in crude extracts using oxaloacetate as substrate was 3.3 U mg-1 of protein. The two isoenzymes had isoelectric points of 6.0 and 7.2, respectiv...

journal_title：Molecular and biochemical parasitology

authors： Uttaro AD,Opperdoes FR

更新日期：1997-10-01 00:00:00

abstract：:It has been shown that vaccination with two low molecular mass excretory secretory (ES) antigens of 15 and 24 kDa, respectively, afforded a substantial degree of protection against Haemonchus contortus to sheep. In vitro cultivation of the parasite usually yields a limited amount of these proteins and therefore, recom...

journal_title：Molecular and biochemical parasitology

authors： Schallig HD,van Leeuwen MA,Verstrepen BE,Cornelissen AW

更新日期：1997-09-01 00:00:00

abstract：:The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly r...

journal_title：Molecular and biochemical parasitology

authors： Ortiz G,Segovia M

更新日期：1996-11-25 00:00:00

abstract：:Amoebiasis is spread by the ingestion of dormant Entamoeba histolytica cysts. Intervention of encystation could break the transmission cycle, thereby reducing disease burden. The model system used to study trophozoite to cyst differentiation is Entamoeba invadens. Here we describe an electroporation-based method for s...

journal_title：Molecular and biochemical parasitology

authors： Singh N,Ojha S,Bhattacharya A,Bhattacharya S

更新日期：2012-07-01 00:00:00

abstract：:Trypanosoma brucei is a unicellular parasite that is transmitted from one mammalian host to the next by tsetse flies. The expression of many trypanosome genes is regulated during the life cycle but there is no evidence for developmental control of transcription by RNA polymerase II. T. brucei expresses at least two he...

journal_title：Molecular and biochemical parasitology

authors： Hotz HR,Lorenz P,Fischer R,Krieger S,Clayton C

更新日期：1995-12-01 00:00:00

abstract：:In addition to their ability to help newly synthesized proteins to fold, molecular chaperones are also recognized for their participation in cellular processes ranging from protein trafficking, signal transduction, differentiation and development. Novel roles for this group of proteins have come to light through studi...

journal_title：Molecular and biochemical parasitology

authors： Acharya P,Kumar R,Tatu U

更新日期：2007-06-01 00:00:00

abstract：:Polymerase chain reaction followed by high resolution melting (PCR-HRM) analysis is a simple, rapid and accurate method for molecular detection of various nematode species. The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A...

journal_title：Molecular and biochemical parasitology

authors： Arbabi M,Hooshyar H,Lotfinia M,Bakhshi MA

更新日期：2020-03-01 00:00:00

abstract：:Apicomplexan parasites utilize a peripheral membrane system called the inner membrane complex (IMC) to facilitate host cell invasion and parasite replication. We recently identified a novel family of Toxoplasma IMC Sub-compartment Proteins (ISP1/2/3) that localize to sub-domains of the IMC using a targeting mechanism ...

journal_title：Molecular and biochemical parasitology

authors： Fung C,Beck JR,Robertson SD,Gubbels MJ,Bradley PJ

更新日期：2012-08-01 00:00:00

abstract：:In trypanosomes and related kinetoplastida a common short 5' leader sequence is encoded by the highly repetitive mini-exon gene and added to all protein coding mRNAs by trans-splicing. To initiate a search for transcription factors associated with transcription of the mini-exon we have used an electrophoretic mobility...

journal_title：Molecular and biochemical parasitology

authors： Koronakis VE,Ross AM,Le Page RW,Hughes C

更新日期：1988-07-01 00:00:00

abstract：:Cathepsin B-like cysteine protease (cbl) genes produce the most abundant mRNAs ( approximately 16%) detected in the adult female intestine of the parasitic nematode Haemonchus contortus. CBL enzymes appear to digest host proteins and are vaccine candidates for immune control of H. contortus and potentially other paras...

journal_title：Molecular and biochemical parasitology

authors： Jasmer DP,Mitreva MD,McCarter JP

更新日期：2004-10-01 00:00:00

abstract：:The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 13C-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 13C-acetate and palmitic-d(31) acid were analyzed...

journal_title：Molecular and biochemical parasitology

authors： Chu FL,Lund E,Soudant P,Harvey E

更新日期：2002-02-01 00:00:00

abstract：:Babesia bovis, an intraerythrocytic, protozoal parasite of cattle, undergoes clonal antigenic variation (Allred DR, Cinque RM, Lane TJ, Ahrens KP. Infect Immun 1994;62:91-98). This ability could provide a mechanism by which the parasite escapes host immune defenses to establish chronic infection. Previous work identif...

journal_title：Molecular and biochemical parasitology

authors： O'Connor RM,Lane TJ,Stroup SE,Allred DR

更新日期：1997-11-01 00:00:00

abstract：:During intracellular development of the malarial parasite numerous membranous vesicles appear in the infected erythrocyte cytoplasm between the parasitophorous vacuolar membrane (PVM) and the erythrocyte plasma membrane. In this study we describe the characterization of a monoclonal antibody which recognizes two major...

journal_title：Molecular and biochemical parasitology

authors： Martinez SL,Clavijo CA,Winograd E

更新日期：1998-03-15 00:00:00

abstract：:The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC 3.6.1.5) which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

journal_title：Molecular and biochemical parasitology

authors： Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

更新日期：2008-12-01 00:00:00

abstract：:The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery ...

journal_title：Molecular and biochemical parasitology

authors： Ismaïli N,Pérez-Morga D,Walsh P,Mayeda A,Pays A,Tebabi P,Krainer AR,Pays E

更新日期：1999-07-30 00:00:00

abstract：:A screen for Leishmania mexicana genes encoding promastigote-specific flagellar proteins resulted in isolation of genes encoding the major components of the paraflagellar rod. One of these, PFR-2, was characterized extensively. PFR-2 genes are present in the genome as a tandem array of three genes designated PFR-2A, P...

journal_title：Molecular and biochemical parasitology

authors： Moore LL,Santrich C,LeBowitz JH

更新日期：1996-10-01 00:00:00

abstract：:The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

journal_title：Molecular and biochemical parasitology

authors： Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

更新日期：2001-08-01 00:00:00

abstract：:A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of ab...

journal_title：Molecular and biochemical parasitology

authors： Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

更新日期：2003-09-01 00:00:00

abstract：:The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

journal_title：Molecular and biochemical parasitology

authors： Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

更新日期：2010-10-01 00:00:00

abstract：:Given the growing appreciation of serious health sequelae from widespread Trichomonas vaginalis infection, new tools are needed to study the parasite's genetic diversity. To this end we have identified and characterized a panel of 21 microsatellites and six single-copy genes from the T. vaginalis genome, using seven l...

journal_title：Molecular and biochemical parasitology

authors： Conrad M,Zubacova Z,Dunn LA,Upcroft J,Sullivan SA,Tachezy J,Carlton JM

更新日期：2011-01-01 00:00:00

abstract：:As an intracellular parasite, Trypanosoma cruzi is exposed to reactive oxygen species. The study of the proteins involved in the hydroperoxide detoxification cascade, tryparedoxin peroxidase included, may lead to the development of a more specific chemotherapy for Chagas'disease. In this work, the involvement of TcCPX...

journal_title：Molecular and biochemical parasitology

authors： Finzi JK,Chiavegatto CW,Corat KF,Lopez JA,Cabrera OG,Mielniczki-Pereira AA,Colli W,Alves MJ,Gadelha FR

更新日期：2004-01-01 00:00:00

abstract：:After a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. The data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. Trypanosomes take up o...

journal_title：Molecular and biochemical parasitology

authors： Fairlamb AH,Bowman IB

更新日期：1980-10-01 00:00:00

abstract：:Recent advances in insect genetic engineering have opened up new possibilities in the genetic control of insect vectors of human diseases. We outline the current state of the sterile insect technique and show how the use of engineered dominant lethals can greatly increase the effectiveness of this approach. We conside...

journal_title：Molecular and biochemical parasitology

authors： Alphey L,Andreasen M

更新日期：2002-05-01 00:00:00