Pyrazolopyrimidine metabolism in African trypanosomes: metabolic similarities to Trypanosoma cruzi and Leishmania spp.


:Growth inhibition and radioisotope incorporation studies with allopurinol (4-hydroxypyrazolo(3,4-d)pyrimidine) have shown that the African trypanosomes are biologically and biochemically similar to Leishmania spp. and Trypanosoma cruzi with respect to their response to this compound. These organisms, as a group, share the unique ability to convert allopurinol sequentially to its ribonucleoside monophosphate and 4-aminopyrazolo(3,4-d)pyrimidine ribonucleoside mono-, di- and triphosphates.


Mol Biochem Parasitol


Berens RL,Marr JJ,Brun R




Has Abstract


1980-04-01 00:00:00














  • Comparative proteomic analysis of antimony-resistant and -susceptible Leishmania braziliensis and Leishmania infantum chagasi lines.

    abstract::The emergence of drug-resistant Leishmania species is a significant problem in several countries. A comparative proteomic analysis of antimony-susceptible and antimony-resistant Leishmania braziliensis (LbSbR) and Leishmania infantum chagasi (LcSbR) lines was carried out using two-dimensional gel electrophoresis (2-DE...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Matrangolo FS,Liarte DB,Andrade LC,de Melo MF,Andrade JM,Ferreira RF,Santiago AS,Pirovani CP,Silva-Pereira RA,Murta SM

    更新日期:2013-08-01 00:00:00

  • Accurate transcription of a cloned gene from Babesia bovis in Saccharomyces cerevisiae.

    abstract::Two cloned chromosomal segments of the hemoprotozoan Babesa bovis bearing genes from the polymorphic locus designated BabR were inserted into plasmid vector YCp19, cloned in Escherichia coli and then introduced into Saccharomyces cerevisiae. Both genes were transcribed producing poly(A+) RNAs. In at least one case, th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kemp DJ,Easton KE,Cowman AF

    更新日期:1984-05-01 00:00:00

  • Sequences with high propensity to form G-quartet structures in kinetoplast DNA from Phytomonas serpens.

    abstract::Naturally occurring sequences containing repetitive guanine motifs have the potential to form tetraplex DNA. Phytomonas serpens minicircle DNA shows some regions where one strand is composed mainly of G and T (GT regions). These regions contain several stretches of contiguous guanines. An oligonucleotide was construct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sá-Carvalho D,Traub-Cseko YM

    更新日期:1995-06-01 00:00:00

  • Characterisation of the two malate dehydrogenases from Phytomonas sp. Purification of the glycosomal isoenzyme.

    abstract::Two NAD(H)-dependent malate dehydrogenase (MDH) isoenzymes were detected in Phytomonas isolated from the lactiferous tubes of Euphorbia characias. The total specific activity in crude extracts using oxaloacetate as substrate was 3.3 U mg-1 of protein. The two isoenzymes had isoelectric points of 6.0 and 7.2, respectiv...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Uttaro AD,Opperdoes FR

    更新日期:1997-10-01 00:00:00

  • A kinetoplast DNA probe diagnostic for Leishmania major: sequence homologies between regions of Leishmania minicircles.

    abstract::A restriction fragment from a cloned kinetoplast minicircle DNA has been shown to be diagnostic for Leishmania major. This 402-bp TaqI fragment has been used routinely (as a radiolabelled probe) to detect 10(4) parasites in simple dot blots, both experimentally and in epidemiological surveys. It positively identified ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Smith DF,Searle S,Ready PD,Gramiccia M,Ben-Ismail R

    更新日期:1989-12-01 00:00:00

  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • Three genes and two isozymes: gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense.

    abstract::The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M

    更新日期:1995-02-01 00:00:00

  • Purification and characterization of three larval excretory-secretory proteins of Dirofilaria immitis.

    abstract::Two proteins were previously described in the excretory-secretory products (ES) collected from Dirofilaria immitis during the molt from the third stage to the fourth stage in vitro. The two proteins were purified using cation exchange and reverse phase HPLC. During the purification of these two proteins, a third prote...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Frank GR,Grieve RB

    更新日期:1996-01-01 00:00:00

  • Genetic and antigenic characterization of Babesia bovis merozoite spherical body protein Bb-1.

    abstract::A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hines SA,Palmer GH,Brown WC,McElwain TF,Suarez CE,Vidotto O,Rice-Ficht AC

    更新日期:1995-02-01 00:00:00

  • Characterization of phosphodiesterase-5 as a surface protein in the tegument of Schistosoma mansoni.

    abstract::Schistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable me...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rofatto HK,Tararam CA,Borges WC,Wilson RA,Leite LC,Farias LP

    更新日期:2009-07-01 00:00:00

  • Functional characterisation of a nematode secreted GM2-activator protein.

    abstract::We have identified a GM2-activator protein (GM2AP) with highly unusual properties secreted by the nematode parasite Trichinella spiralis. Expression in Pichia pastoris resulted in a hyperglycosylated protein of 28 kDa, but the 18 kDa native protein was not glycosylated. The parasite GM2AP does not facilitate degradati...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bruce AF,Gares MP,Selkirk ME,Gounaris K

    更新日期:2006-06-01 00:00:00

  • A gene-family encoding small exported proteins is conserved across Plasmodium genus.

    abstract::A gene-family, named sep, encoding small exported proteins conserved across Plasmodium species has been identified. SEP proteins (13-16 kDa) contain a predicted signal peptide at the NH(2)-terminus, an internal hydrophobic region and a polymorphic, low-complexity region at the carboxy-terminus. One member of the Plasm...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

    更新日期:2003-02-01 00:00:00

  • Identification by extrachromosomal amplification and overexpression of a zeta-crystallin/NADPH-oxidoreductase homologue constitutively expressed in Leishmania spp.

    abstract::A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liu X,Chang KP

    更新日期:1994-08-01 00:00:00

  • Protein variation in clones of Plasmodium falciparum detected by two dimensional electrophoresis.

    abstract::Two dimensional electrophoresis has been used to examine protein variation in clones of two Plasmodium falciparum isolates. Variant forms of 12 proteins were detected. Five genetically distinct parasite types were identified in one isolate, and two in the second isolate. Examination of uncloned parasites using this te...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fenton B,Walker A,Walliker D

    更新日期:1985-08-01 00:00:00

  • Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues.

    abstract::The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cameron S,Fyffe SA,Goldie S,Hunter WN

    更新日期:2008-04-01 00:00:00

  • Cloning of a cDNA encoding the dense granule protein GRA3 from Toxoplasma gondii.

    abstract::GRA3 is a 30-kDa protein located inside the dense granules of Toxoplasma gondii. Following invasion and exocytosis of dense granules within the parasitophorous vacuole, GRA3 becomes associated with the parasitophorous vacuolar membrane (PVM) and extensions of the PVM which protrude into the cytoplasm. A partial cDNA e...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bermudes D,Dubremetz JF,Achbarou A,Joiner KA

    更新日期:1994-12-01 00:00:00

  • The two mechanisms for antigenic variation in Trypanosoma brucei are independent processes.

    abstract::Antigenic switching in Trypanosoma brucei can occur either by the production of a telomeric copy of a variant surface glycoprotein (VSG) gene through a gene conversion mechanism or by the nonduplicative activation of a telomeric VSG gene. The 5 VSG gene telomeric copy that is expressed in IsTaR 1 variant antigenic typ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Aline RF Jr,Stuart K

    更新日期:1985-06-01 00:00:00

  • cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite.

    abstract::A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

    更新日期:1995-07-01 00:00:00

  • Structural characterisation and pharmacology of KHEYLRFamide (AF2) and KSAYMRFamide (PF3/AF8) from Haemonchus contortus.

    abstract::The FMRFamide-related peptides (FaRPs), KHEYLRFamide (AF2) and KSAYMRFamide (PF3) were structurally characterised from the parasitic nematode of sheep, Haemonchus contortus (MH isolate). Both peptides were sequenced in a single gas-phase sequencing run and their structure confirmed by mass spectrometry which identifie...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marks NJ,Sangster NC,Maule AG,Halton DW,Thompson DP,Geary TG,Shaw C

    更新日期:1999-05-25 00:00:00

  • The varieties of gene amplification, diversification and hypervariability in the human malaria parasite, Plasmodium falciparum.

    abstract::The human malaria parasite, Plasmodium falciparum, is able to evade host cell-mediated and humoral immunity to maintain both persistent and repeated infections. Immune evasion is in part due to a robust repertoire of proteins which participate in host-parasite interactions but also exhibit profound antigenic diversity...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Templeton TJ

    更新日期:2009-08-01 00:00:00

  • Isolation and characterisation of ribosomal RNA from the human malaria parasite Plasmodium falciparum.

    abstract::Ribosomal RNA isolated from the malaria parasite Plasmodium falciparum consists of two species with molecular weight of 1.49 +/- 0.09 X 10(6) and 0.78 +/- 0.02 X 10(6) present in equimolar quantities. Their molecular weights are comparable with those of other protozoa but quite distinct from those of the human host. T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hyde JE,Zolg JW,Scaife JG

    更新日期:1981-12-31 00:00:00

  • 3-Methyladenine blocks Toxoplasma gondii division prior to centrosome replication.

    abstract::The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

    更新日期:2010-10-01 00:00:00

  • A phylogenetic analysis of the Trypanosoma cruzi genome project CL Brener reference strain by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting.

    abstract::We have assessed the phylogenetic status of the Trypanosoma cruzi Genome Project CL Brener reference strain by multilocus enzyme electrophoresis (MLEE) and multiprimer random amplified polymorphic DNA (RAPD) including a set of cloned stocks representative of the whole genetic diversity of T. cruzi. MLEE and RAPD data ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Brisse S,Barnabé C,Bañuls AL,Sidibé I,Noël S,Tibayrenc M

    更新日期:1998-05-01 00:00:00

  • A high level of mixed Trypanosoma brucei infections in tsetse flies detected by three hypervariable minisatellites.

    abstract::The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: MacLeod A,Turner CM,Tait A

    更新日期:1999-08-20 00:00:00

  • Characterization of a Giardia lamblia variant-specific surface protein (VSP) gene from isolate GS/M and estimation of the VSP gene repertoire size.

    abstract::Giardia lamblia undergoes surface antigenic variation. The variant-specific surface proteins (VSPs) of isolate WB are cysteine-rich, can vary dramatically in size, contain Cys-X-X-Cys motifs, and are differentially expressed. GS/M(H7) is a Giardia clone from a different isolate which expresses a VSP epitope not found ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nash TE,Mowatt MR

    更新日期:1992-04-01 00:00:00

  • The fate of ferriprotorphyrin IX in malaria infected erythrocytes in conjunction with the mode of action of antimalarial drugs.

    abstract::The intraerythrocytic malaria parasite digests considerable amounts of its host cell cytosol, which consists mostly of hemoglobin. In order to avert the toxicity of ferriprotorphyrin IX (FP) thus produced, it is generally accepted that FP is polymerized to the non-toxic hemozoin. Investigating the fate of FP in cultur...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zhang J,Krugliak M,Ginsburg H

    更新日期:1999-03-15 00:00:00

  • Duplication, gene conversion, and genetic diversity in the species-specific acyl-CoA synthetase gene family of Plasmodium falciparum.

    abstract::While genes encoding antigens and other highly polymorphic proteins are commonly found in subtelomeres, it is unusual to find a small family of housekeeping genes in these regions. We found that in the species Plasmodium falciparum only, a non-subtelomeric acyl-CoA synthetase (ACS) gene has expanded into a family of d...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bethke LL,Zilversmit M,Nielsen K,Daily J,Volkman SK,Ndiaye D,Lozovsky ER,Hartl DL,Wirth DF

    更新日期:2006-11-01 00:00:00

  • Genetic variability and evolution of the Schistosoma genome analysed by using random amplified polymorphic DNA markers.

    abstract::The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barral V,This P,Imbert-Establet D,Combes C,Delseny M

    更新日期:1993-06-01 00:00:00

  • The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei.

    abstract::Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gao F,Voncken F,Colasante C

    更新日期:2020-05-01 00:00:00

  • Identification of Eimeria bovis merozoite cDNAs using differential mRNA display.

    abstract::Differences in gene expression between Eimeria bovis sporozoites and first-generation merozoites were analyzed using the technique of differential mRNA display. Approx. 5% of the sequences detected in first-generation merozoites appear to be unique relative to sporozoites. Several of the bands corresponding to merozoi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Abrahamsen MS,Johnson RR,Hathaway M,White MW

    更新日期:1995-05-01 00:00:00