Population genetic analysis and sub-structuring in Babesia bovis.


:The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to investigate the role of genetic exchange in the population structure and diversity of the parasite using isolates from Zambia and Turkey. This population genetic analysis showed that genetic exchange occurs and that there are high levels of genetic diversity, with geographical sub-structuring quantified using Wright's F Index. Linkage disequilibrium was observed when isolates from both countries were treated as one population, but when isolates from Zambia were analysed separately linkage equilibrium was observed. The Turkish isolates were sub-structured, containing two genetically distinct sub-groups, both of which appeared to be in linkage equilibrium. The results of the Zambian study suggest that a sub-set of the parasite population is responsible for the westward spread of babesiosis into the previously disease-free central region of the country. The Zambian isolates had a significantly higher number of genotypes per sample than those from Turkey and age was found to be a significant predictor of the multiplicity of infection. The high levels of diversity seen in the Zambian and Turkish B. bovis populations have implications in the development of subunit vaccines against the disease and the spread of drug resistance.


Mol Biochem Parasitol


Simuunza M,Bilgic H,Karagenc T,Syakalima M,Shiels B,Tait A,Weir W




Has Abstract


2011-06-01 00:00:00














  • The pir multigene family of Plasmodium: antigenic variation and beyond.

    abstract::Multigene families are present on the telomeric and sub-telomeric regions of most chromosomes of the malaria parasite, Plasmodium. The largest gene family identified so far is the Plasmodium interspersed repeat (pir) multigene gene family and is shared by Plasmodium vivax, and simian and rodent malaria species. Most p...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Cunningham D,Lawton J,Jarra W,Preiser P,Langhorne J

    更新日期:2010-04-01 00:00:00

  • Characterization of phosphodiesterase-5 as a surface protein in the tegument of Schistosoma mansoni.

    abstract::Schistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable me...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rofatto HK,Tararam CA,Borges WC,Wilson RA,Leite LC,Farias LP

    更新日期:2009-07-01 00:00:00

  • Characterisation of the two malate dehydrogenases from Phytomonas sp. Purification of the glycosomal isoenzyme.

    abstract::Two NAD(H)-dependent malate dehydrogenase (MDH) isoenzymes were detected in Phytomonas isolated from the lactiferous tubes of Euphorbia characias. The total specific activity in crude extracts using oxaloacetate as substrate was 3.3 U mg-1 of protein. The two isoenzymes had isoelectric points of 6.0 and 7.2, respectiv...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Uttaro AD,Opperdoes FR

    更新日期:1997-10-01 00:00:00

  • Cysteine proteinase of Entamoeba histolytica. I. Partial purification and action on different enzymes.

    abstract::A method for a 50-60-fold purification of a cysteine proteinase from trophozoites of Entamoeba histolytica using 35-80% ammonium sulphate fractionation, gel chromatography on Sephadex G-75, and preparative isoelectric focusing is described. The enzyme was examined for its proteolytic potencies towards native enzyme su...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Scholze H,Werries E

    更新日期:1986-01-01 00:00:00

  • Polymorphism of proteins in malaria parasites following mefloquine treatment.

    abstract::Proteins in malaria parasites (Plasmodium falciparum) isolated from a patient in Thailand before treatment, and after recrudescence of infection subsequent to mefloquine treatment, were compared by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis. Nine 'pre-treatment' and six 'recrudescent' clones...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pinswasdi C,Thaithong S,Beale GH,Fenton B,Webster HK,Pavanand K

    更新日期:1987-03-01 00:00:00

  • Chaperoning a cellular upheaval in malaria: heat shock proteins in Plasmodium falciparum.

    abstract::In addition to their ability to help newly synthesized proteins to fold, molecular chaperones are also recognized for their participation in cellular processes ranging from protein trafficking, signal transduction, differentiation and development. Novel roles for this group of proteins have come to light through studi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Acharya P,Kumar R,Tatu U

    更新日期:2007-06-01 00:00:00

  • Demonstration of glycosomes (microbodies) in the Bodonid flagellate Trypanoplasma borelli (Protozoa, Kinetoplastida).

    abstract::Homogenates of Trypanoplasma borelli were subjected to subcellular fractionation by sequential differential and isopycnic centrifugation in sucrose. Glycerol-3-phosphate dehydrogenase and the glycolytic enzymes, glucosephosphate isomerase and triosephosphate isomerase, as well as the peroxisomal marker enzyme catalase...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Opperdoes FR,Nohynkova E,Van Schaftingen E,Lambeir AM,Veenhuis M,Van Roy J

    更新日期:1988-08-01 00:00:00

  • Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei.

    abstract::The glycosylphosphatidylinositol phospholipase C (GPI-PLC) from Trypanosoma brucei is particularly effective in hydrolysing the GPI-anchors of some proteins. The enzyme is inhibited by Zn2+ and p-chloromercurylphenylsulphonic acid, both of which can act as sulphydryl reagents, suggesting that a cysteine residue may be...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carnall N,Webb H,Carrington M

    更新日期:1997-12-15 00:00:00

  • Characterisation of the loci encoding the glutamic acid and alanine rich protein of Trypanosoma congolense.

    abstract::We have characterised the organisation of genes encoding the glutamate and alanine rich protein (GARP) surface coat of the procyclic and epimastigote stages of Trypanosoma congolense in the tsetse fly. The GARP genes are arranged at two, possibly physically linked, loci, one of which exhibits allelic variation. One lo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rangarajan D,Harvey TI,Barry JD

    更新日期:2000-02-05 00:00:00

  • Arp1, an actin-related protein, in Plasmodium berghei.

    abstract::Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Siden-Kiamos I,Schüler H,Liakopoulos D,Louis C

    更新日期:2010-10-01 00:00:00

  • Electron transfer complexes of Ascaris suum muscle mitochondria: I. Characterization of NADH-cytochrome c reductase (complex I-III), with special reference to cytochrome localization.

    abstract::An NADH-cytochrome c reductase (complex I-III) was isolated from Ascaris suum muscle mitochondria. The enzyme preparation catalyzed the reduction of 1.68 mumol cytochrome c min-1 mg-1 protein at 25 degrees C with NADH but not with NADPH, and retained its sensitivity to rotenone, piericidin A and 2-heptyl-4-hydroxyquin...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Takamiya S,Furushima R,Oya H

    更新日期:1984-10-01 00:00:00

  • The Haemonchus contortus UNC-49B subunit possesses the residues required for GABA sensitivity in homomeric and heteromeric channels.

    abstract::Hco-UNC-49 is a GABA receptor from the parasitic nematode Haemonchus contortus that has a relatively low overall sequence similarity to vertebrate GABA receptors but is very similar to the UNC-49 receptor found in the free living nematode Caenorhabditis elegans. While the nematode receptors do share >80% sequence simi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Accardi MV,Forrester SG

    更新日期:2011-07-01 00:00:00

  • Identification of surface membrane proteins and surface membrane antigens of plasmodium chabaudi merozoites.

    abstract::Surface membrane proteins of viable merozoites of Plasmodium chabaudi were iodinated by the Iodogen method and analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirteen surface membrane proteins ranging from 22 to 270 kDa were thus identified. Most of these proteins could be immunoprecipitated by...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Franco da Silveira J,Falanga PB,Pereira da Silva L

    更新日期:1984-01-01 00:00:00

  • Insights into blood feeding by schistosomes from a proteomic analysis of worm vomitus.

    abstract::Whilst the schistosome tegument has been intensively studied there is little information about processes in the gut, the other major interface with the bloodstream, apart from the well characterised cascade of proteases involved in haemoglobin digestion. To gain insights into gut function we undertook a proteomic anal...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hall SL,Braschi S,Truscott M,Mathieson W,Cesari IM,Wilson RA

    更新日期:2011-09-01 00:00:00

  • Linearized free maxicircle DNA in Crithidia fasciculata is a product of topoisomerase II-mediated cleavage.

    abstract::Linearized free maxicircle DNA, present in detergent lysates of Crithidia fasciculata mitochondria, was thought to be a replication intermediate formed during rolling circle replication of maxicircle DNA. Gel electrophoresis of the linearized free maxicircles indicated that they were slightly larger than the maxicircl...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carpenter LR,Englund PT

    更新日期:1996-02-01 00:00:00

  • Identification of Entamoeba histolytica thiol-specific antioxidant as a GalNAc lectin-associated protein.

    abstract::Entamoeba histolytica is a human intestinal parasite that causes amebic dysentery. A cell surface amebic adhesin, the galactose and N-acetyl-D-galactosamine inhibitable (GalNAc) lectin mediates amebic adherence to and contact-dependent killing of host cells. Previous work has suggested that the GalNAc lectin transduce...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hughes MA,Lee CW,Holm CF,Ghosh S,Mills A,Lockhart LA,Reed SL,Mann BJ

    更新日期:2003-04-03 00:00:00

  • 3-Methyladenine blocks Toxoplasma gondii division prior to centrosome replication.

    abstract::The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

    更新日期:2010-10-01 00:00:00

  • Molecular characterization of bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium falciparum.

    abstract::A 2118-base pair gene encoding the bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate syntheses of Plasmodium falciparum (pfPPPK-DHPS) was expressed under the control of the T5 promoter in a DHPS-deficient Escherichia coli strain. The enzyme was purified to near homogeneity using nickel affinity...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kasekarn W,Sirawaraporn R,Chahomchuen T,Cowman AF,Sirawaraporn W

    更新日期:2004-09-01 00:00:00

  • RNAi interference of XPO1 and Sm genes and their effect on the spliced leader RNA in Trypanosoma brucei.

    abstract::In trypanosomes, trans-splicing is a major essential RNA-processing mechanism that involves the addition of a spliced leader sequence to all mRNAs from a small RNA species, known as the spliced leader RNA (SL RNA). SL RNA maturation is poorly understood and it is not clear where assembly with Sm proteins takes place. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Biton M,Mandelboim M,Arvatz G,Michaeli S

    更新日期:2006-12-01 00:00:00

  • An S antigen gene from Plasmodium falciparum contains a novel repetitive sequence.

    abstract::The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

    更新日期:1988-02-01 00:00:00

  • The FAR proteins of filarial nematodes: secretion, glycosylation and lipid binding characteristics.

    abstract::The FAR proteins of nematodes are small ( approximately 20 kDa), helix-rich, fatty acid and retinol-binding (FAR) proteins that appear to be confined to nematodes. We have carried out a comparative sequence and biochemical analysis of selected FAR proteins often species of filarial parasites (from the genera Onchocerc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Garofalo A,Kläger SL,Rowlinson MC,Nirmalan N,Klion A,Allen JE,Kennedy MW,Bradley JE

    更新日期:2002-07-01 00:00:00

  • Theileria parva 104 kDa microneme--rhoptry protein is membrane-anchored by a non-cleaved amino-terminal signal sequence for entry into the endoplasmic reticulum.

    abstract::The 104 kDa microneme-rhoptry protein (p104) is the only known apical complex organelle-specific protein of Theileria parva. p104 exhibits striking structural similarities to circumsporozoite protein and sporozoite surface protein 2 of Plasmodium yoelii. Their primary sequences contain two hydrophobic segments, locate...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ebel T,Gerhards J,Binder BR,Lipp J

    更新日期:1999-05-15 00:00:00

  • L-myo-Inositol 1-phosphate synthase from Entamoeba histolytica.

    abstract::L-myo-Inositol 1-phosphate synthase (I-1-P synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. Inositol is a precursor of essential macromolecules like membrane phospholipids, GPI anchor proteins and lipophos...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lohia A,Hait NC,Majumder AL

    更新日期:1999-01-05 00:00:00

  • Characterization of the Trypanosoma brucei homologue of a Trypanosoma cruzi flagellum-adhesion glycoprotein.

    abstract::An immunodominant 72-kDa surface glycoprotein (Gp72) of Trypanosoma cruzi is involved in adhesion of the flagellum to the cell body (Cooper, R, Ribeiro de Jesus, A and Cross, G.A.M (1993) J. Cell Biol. 122, 149-156). We have characterized a gene, flagellum-adhesion glycoprotein genel (fla1), from Trypanosoma brucei th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nozaki T,Haynes PA,Cross GA

    更新日期:1996-11-25 00:00:00

  • Translational repression controls temporal expression of the Plasmodium berghei LCCL protein complex.

    abstract::Plasmodium LCCL proteins comprise a family of six proteins that function as a protein complex and have essential roles in sporozoite transmission. In Plasmodium berghei, family members PbLAP1, PbLAP2 and PbLAP3 have been shown to be expressed in gametocytes and, following gametogenesis and fertilization, to be targete...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Saeed S,Carter V,Tremp AZ,Dessens JT

    更新日期:2013-05-01 00:00:00

  • TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei.

    abstract::The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contras...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Price HP,Stark M,Smith B,Smith DF

    更新日期:2007-10-01 00:00:00

  • Characterization of the complete mitogenome of Centrorhynchus clitorideus (Meyer, 1931) (Palaeacanthocephala: Centrorhynchidae), the largest mitochondrial genome in Acanthocephala, and its phylogenetic implications.

    abstract::Species of Centrorhynchus (Polymorphida: Centrorhynchidae) commonly parasitize various falconiform and strigiform birds worldwide. In the present study, the complete mitochondrial (mt) genome sequences of Centrorhynchus clitorideus was sequenced and annotated for the first time based on specimens collected from the li...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Muhammad N,Suleman,Khan MS,Li L,Zhao Q,Ullah H,Zhu XQ,Ma J

    更新日期:2020-05-01 00:00:00

  • Identification and expression analysis of ABC protein-encoding genes in Toxoplasma gondii. Toxoplasma gondii ATP-binding cassette superfamily.

    abstract::The ATP-binding cassette (ABC) transporters are one of the largest evolutionarily conserved families of proteins. They are characterized by the presence of nucleotide-binding domains (NBDs), which are highly conserved among organisms. In the present study, we used human and protozoan ABC sequences, and ATP-binding con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sauvage V,Millot JM,Aubert D,Visneux V,Marle-Plistat M,Pinon JM,Villena I

    更新日期:2006-06-01 00:00:00

  • 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci.

    abstract::The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

    更新日期:2008-12-01 00:00:00

  • Mechanisms of respiration and phosphorylation in Ascaris muscle mitochondria.

    abstract::In Ascaris muscle mitochondria the major respiratory chain-linked phosphorylation activity is accomplished by a NADH-linked reduction of fumarate to succinate. Oxygen can also be employed as a terminal electron acceptor via a cyanide- and salicyl-hydroxamate-resistant terminal oxidase. As in fumarate-dependent electro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Köhler P,Bachmann R

    更新日期:1980-04-01 00:00:00