Population genetic analysis and sub-structuring in Babesia bovis.


:The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to investigate the role of genetic exchange in the population structure and diversity of the parasite using isolates from Zambia and Turkey. This population genetic analysis showed that genetic exchange occurs and that there are high levels of genetic diversity, with geographical sub-structuring quantified using Wright's F Index. Linkage disequilibrium was observed when isolates from both countries were treated as one population, but when isolates from Zambia were analysed separately linkage equilibrium was observed. The Turkish isolates were sub-structured, containing two genetically distinct sub-groups, both of which appeared to be in linkage equilibrium. The results of the Zambian study suggest that a sub-set of the parasite population is responsible for the westward spread of babesiosis into the previously disease-free central region of the country. The Zambian isolates had a significantly higher number of genotypes per sample than those from Turkey and age was found to be a significant predictor of the multiplicity of infection. The high levels of diversity seen in the Zambian and Turkish B. bovis populations have implications in the development of subunit vaccines against the disease and the spread of drug resistance.


Mol Biochem Parasitol


Simuunza M,Bilgic H,Karagenc T,Syakalima M,Shiels B,Tait A,Weir W




Has Abstract


2011-06-01 00:00:00














  • Trypanosoma cruzi attachment to lymphocyte muscarinic cholinergic and beta adrenergic receptors modulates intracellular signal transduction.

    abstract::Plasma membrane vesicles of Trypanosoma cruzi (PMVs) formed saturation binding isotherms with naive murine T lymphocytes. Parasite membrane attachment to the muscarinic cholinergic receptors of Lyt 2.2+T cells (suppressor cells) resulted in the synthesis of cGMP, attenuation of cAMP levels and in the secretion of pros...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Borda ES,Sterin-Borda LJ,Pascual JO,Gorelik G,Felix JC,von Kreuter BF,Santos-Buch CA

    更新日期:1991-07-01 00:00:00

  • Transgenic Plasmodium berghei sporozoites expressing beta-galactosidase for quantification of sporozoite transmission.

    abstract::Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Engelmann S,Sinnis P,Matuschewski K

    更新日期:2006-03-01 00:00:00

  • Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana.

    abstract::Glucose is a major source of energy and carbon in promastigotes of Leishmania mexicana, and its uptake is mediated by three glucose transporters whose genes are encoded within a single cluster. A null mutant in which the glucose transporter gene cluster was deleted by homologous gene replacement was generated previous...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rodriguez-Contreras D,Feng X,Keeney KM,Bouwer HG,Landfear SM

    更新日期:2007-05-01 00:00:00

  • Characterization and developmental gene regulation of a large gene family encoding amastin surface proteins in Leishmania spp.

    abstract::The ability of Leishmania amastigotes to survive within the drastic environmental changes encountered in the phagolysosomes of mammalian macrophages is heavily dependent on the developmental regulation of a variety of genes. The identification of genes that are expressed preferentially in the mammalian stage of the pa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rochette A,McNicoll F,Girard J,Breton M,Leblanc E,Bergeron MG,Papadopoulou B

    更新日期:2005-04-01 00:00:00

  • Generation and analysis of expressed sequence tags from Trypanosoma cruzi trypomastigote and amastigote cDNA libraries.

    abstract::We have generated 2771 expressed sequence tags (ESTs) from two cDNA libraries of Trypanosoma cruzi CL-Brener. The libraries were constructed from trypomastigote and amastigotes, using a spliced leader primer to synthesize the cDNA second strand, thus selecting for full-length cDNAs. Since the libraries were not normal...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Agüero F,Abdellah KB,Tekiel V,Sánchez DO,González A

    更新日期:2004-08-01 00:00:00

  • Control of gene expression in Plasmodium falciparum.

    abstract::Transfection has facilitated a functional analysis of transcriptional processes in the human malarial parasite Plasmodium falciparum, providing the first fascinating glimpses into the mechanisms regulating parasite development and pathogenicity. Here we review our rapidly evolving knowledge of what constitutes a promo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Horrocks P,Dechering K,Lanzer M

    更新日期:1998-09-15 00:00:00

  • Crystal structure of the C-terminal domain of tubulin-binding cofactor C from Leishmania major.

    abstract::Tubulin-binding cofactor C stimulates GTPase activity and contributes to the release of the heterodimeric α/β-tubulin from a super-complex of tubulin monomers and two ancillary cofactors. We have determined the 2.2 Å resolution crystal structure of the C-terminal domain of tubulin-binding cofactor C from Leishmania ma...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barrack KL,Fyfe PK,Finney AJ,Hunter WN

    更新日期:2015-05-01 00:00:00

  • Organization, sequence and stage-specific expression of the phosphoglycerate kinase genes of Leishmania mexicana mexicana.

    abstract::In Leishmania mexicana two genes were detected coding for different isoforms of the glycolytic enzyme phosphoglycerate kinase. This situation contrasts with that observed in other Trypanosomatidae (Trypanosoma brucei, Trypanosoma congolense, Crithidia fasciculata) analyzed previously, which all contain three different...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Adjé CA,Opperdoes FR,Michels PA

    更新日期:1997-12-01 00:00:00

  • Thiol groups on the surface of anaerobic parasitic protozoa.

    abstract::Evidence is presented that Giardia lamblia and Entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. Both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gillin FD,Reiner DS,Levy RB,Henkart PA

    更新日期:1984-09-01 00:00:00

  • Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis.

    abstract::The cysteine proteinase B of Leishmania parasites is an important virulence factor. In this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative agent of mucocutaneous leishmaniosis. The mature region of the recombinant CPB shares a high p...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lanfranco MF,Loayza-Muro R,Clark D,Núñez R,Zavaleta AI,Jimenez M,Meldal M,Coombs GH,Mottram JC,Izidoro M,Juliano MA,Juliano L,Arévalo J

    更新日期:2008-10-01 00:00:00

  • The influence of temperature and serum deprivation on the synthesis of heat-shock proteins and alpha and beta tubulin in promastigotes of Leishmania major.

    abstract::We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthe...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Toye P,Remold H

    更新日期:1989-06-01 00:00:00

  • ABC transporters in the liver fluke Opisthorchis felineus.

    abstract::ATP-binding cassette transporters (ABC transporters) are essential components of normal cellular physiological machinery in all eukaryotic and prokaryotic species, including parasites. Some ABC transporters, e.g., P-glycoproteins, are involved in the efflux of toxins and xenobiotics from the cell. At present, nothing ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mordvinov VA,Ershov NI,Pirozhkova DS,Pakharukov YV,Pakharukova MY

    更新日期:2017-09-01 00:00:00

  • Two-dimensional electrophoretic separation and fluorographic analysis of the gene products synthesized by Hymenolepis diminuta with particular reference to the parasite-specific polypeptides in the brush border membrane.

    abstract::This study is the first demonstration of individual gene products synthesized by Hymenolepis diminuta. To identify proteins synthesized specifically by H. diminuta, we radiolabeled adult worms with [14C]leucine in vitro. Subsequent one- and two-dimensional gel electrophoretic separation and staining of the protein con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Atkinson BG,Podesta RB

    更新日期:1982-07-01 00:00:00

  • Parasitism and chromosome dynamics in protozoan parasites: is there a connection?

    abstract::Genomic plasticity is a hallmark of many protozoan parasites, including Plasmodium spp, Trypanosoma spp, Leishmania ssp and Giardia lamblia. Strikingly, there is a common theme regarding the structural basis of this karyotype variability. Chromosomes are compartmentalized into conserved central domains and polymorphic...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Lanzer M,Fischer K,Le Blancq SM

    更新日期:1995-03-01 00:00:00

  • Isolation and characterization of infective and non-infective clones of Leishmania tropica.

    abstract::Cloning by limit dilution of an isolate of Leishmania tropica (LRC-L137) that is infective for mice resulted in 7 stable clones, only one of which was infective in BALB/c mice. Three of the non-infective clones that were examined for survival in BALB/c macrophages in vitro seemed to be killed more readily, suggesting ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Hocking RE,Mitchell GF,Spithill TW

    更新日期:1983-02-01 00:00:00

  • Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum: different logics achieve the same goal.

    abstract::Babesia bovis is a protozoal hemoparasite of cattle which behaves in certain crucial respects like Plasmodium falciparum, despite being phylogenetically distant and having many differences in its life cycle. The shared behavioral attributes of rapid antigenic variation and cytoadhesion/sequestration are thought to con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Allred DR,Al-Khedery B

    更新日期:2004-03-01 00:00:00

  • Electron transfer complexes of Ascaris suum muscle mitochondria: I. Characterization of NADH-cytochrome c reductase (complex I-III), with special reference to cytochrome localization.

    abstract::An NADH-cytochrome c reductase (complex I-III) was isolated from Ascaris suum muscle mitochondria. The enzyme preparation catalyzed the reduction of 1.68 mumol cytochrome c min-1 mg-1 protein at 25 degrees C with NADH but not with NADPH, and retained its sensitivity to rotenone, piericidin A and 2-heptyl-4-hydroxyquin...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Takamiya S,Furushima R,Oya H

    更新日期:1984-10-01 00:00:00

  • Molecular characterization of a thrombospondin-related anonymous protein homologue in Neospora caninum.

    abstract::Thrombospondin-related anonymous protein (TRAP) family members participate in attachment and invasion of host cells by apicomplexan parasites. A TRAP homologue in Neospora caninum strain Nc-1 (NcMIC2) was cloned, sequenced and found to be 61% identical (75% similar) at the amino acid level to Toxoplasma gondii MIC2 (T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lovett JL,Howe DK,Sibley LD

    更新日期:2000-03-15 00:00:00

  • Antisense RNA and RNAi in protozoan parasites: working hard or hardly working?

    abstract::The complex life cycles of many protozoan parasites require the ability to respond to environmental and developmental cues through regulated gene expression. Traditionally, parasitologists have investigated these mechanisms by identifying and characterizing proteins that are necessary for the regulated expression of t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Militello KT,Refour P,Comeaux CA,Duraisingh MT

    更新日期:2008-02-01 00:00:00

  • Regions in the 3' untranslated region confer stage-specific expression to the Leishmania mexicana a600-4 gene.

    abstract::Protozoan parasites of the genus Leishmania have a digenetic lifecycle, alternating between the promastigote and amastigote stages. The extracellular promastigote resides within a sandfly vector, while the obligate intracellular amastigote stage replicates in the phagolysosome of mammalian host macrophages. Adaptation...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Murray A,Fu C,Habibi G,McMaster WR

    更新日期:2007-06-01 00:00:00

  • A Plasmodium falciparum isolate with a chromosome 9 deletion expresses a trypsin-resistant cytoadherence molecule.

    abstract::Sequestration of Plasmodium falciparum infected erythrocytes in the cerebral circulation is strongly implicated in the pathogenesis of cerebral malaria. From previous studies it was postulated that genes essential for cytoadherence were located on the right arm of chromosome 9 as P. falciparum isolates with a deletion...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chaiyaroj SC,Coppel RL,Magowan C,Brown GV

    更新日期:1994-09-01 00:00:00

  • An evaluation of different methods for labelling the surface of the filarial nematode Brugia pahangi with 125iodine.

    abstract::The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marshall E,Howells RE

    更新日期:1985-06-01 00:00:00

  • Translational repression controls temporal expression of the Plasmodium berghei LCCL protein complex.

    abstract::Plasmodium LCCL proteins comprise a family of six proteins that function as a protein complex and have essential roles in sporozoite transmission. In Plasmodium berghei, family members PbLAP1, PbLAP2 and PbLAP3 have been shown to be expressed in gametocytes and, following gametogenesis and fertilization, to be targete...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Saeed S,Carter V,Tremp AZ,Dessens JT

    更新日期:2013-05-01 00:00:00

  • Efficient extraction and translation of Plasmodium falciparum messenger RNA.

    abstract::A convenient method was employed for the efficient extraction of total RNA from Plasmodium falciparum. By depleting the total RNA of tRNA, it was shown that P. falciparum or P. lophurae tRNAs markedly stimulate the translation of P. falciparum mRNA in the rabbit reticulocyte cell-free system. Analysis of the cell-free...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wallach M

    更新日期:1982-12-01 00:00:00

  • The enzymes of pyrimidine biosynthesis in a range of parasitic protozoa and helminths.

    abstract::The activities of carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase, five of the six enzymes of pyrimidine biosynthesis, have been measured in Crithidia fasciculata, Trypanosoma cruzi, Leishmania major, Trichomonas vagi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hill B,Kilsby J,Rogerson GW,McIntosh RT,Ginger CD

    更新日期:1981-02-01 00:00:00

  • Susceptibility of Plasmodium falciparum to glutamate dehydrogenase inhibitors--a possible new antimalarial target.

    abstract::With the rapid spread of drug-resistant strains of Plasmodium falciparum, the development of new antimalarials is an urgent need. As malaria parasites live in a highly pro-oxidant environment, their anti-oxidant defences have frequently been suggested as candidate drug targets. A key point in such defences is the prod...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Aparicio IM,Marín-Menéndez A,Bell A,Engel PC

    更新日期:2010-08-01 00:00:00

  • Cysteine proteinase of Entamoeba histolytica. I. Partial purification and action on different enzymes.

    abstract::A method for a 50-60-fold purification of a cysteine proteinase from trophozoites of Entamoeba histolytica using 35-80% ammonium sulphate fractionation, gel chromatography on Sephadex G-75, and preparative isoelectric focusing is described. The enzyme was examined for its proteolytic potencies towards native enzyme su...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Scholze H,Werries E

    更新日期:1986-01-01 00:00:00

  • Expression differential of microsomal and cytosolic glutathione-S-transferases in Fasciola hepatica resistant at triclabendazole.

    abstract::In the present work, we evaluate in vitro the cytosolic and microsomal activity of glutathione-S-transferases in adults of Fasciola hepatica susceptible (Cullompton Strain) and resistant (Sligo Strain) to triclabendazole. The triclabendazole resistant (Sligo) fluke expressed significant major metabolic activity of glu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Scarcella S,Lamenza P,Virkel G,Solana H

    更新日期:2012-01-01 00:00:00

  • Trypanosoma cruzi response to the oxidative stress generated by hydrogen peroxide.

    abstract::As an intracellular parasite, Trypanosoma cruzi is exposed to reactive oxygen species. The study of the proteins involved in the hydroperoxide detoxification cascade, tryparedoxin peroxidase included, may lead to the development of a more specific chemotherapy for Chagas'disease. In this work, the involvement of TcCPX...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Finzi JK,Chiavegatto CW,Corat KF,Lopez JA,Cabrera OG,Mielniczki-Pereira AA,Colli W,Alves MJ,Gadelha FR

    更新日期:2004-01-01 00:00:00

  • The P-glycoprotein-related gene family in Leishmania.

    abstract::P-glycoprotein gene amplification has been described in several drug-resistant parasitic protozoa. The first P-glycoprotein related gene described in Leishmania was ltpgpA, a gene frequently amplified in arsenite resistant Leishmania. Hybridization experiments indicated that ltpgpA was part of a gene family. In additi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Légaré D,Hettema E,Ouellette M

    更新日期:1994-11-01 00:00:00