当前位置： SCI文献检索 > MOLECULAR AND BIOCHEMICAL PARASITOLOGY期刊下所有文献 > Effects of hypoxia and acute osmotic stress on intermediary metabolism in Leishmania promastigotes.

Effects of hypoxia and acute osmotic stress on intermediary metabolism in Leishmania promastigotes.

Abstract：

:This study further explores the effects of hypoxia and acute osmotic stress on intermediary metabolism of Leishmania major and Leishmania donovani. Late log phase promastigotes were washed and incubated with glucose as the sole exogenous carbon source, and rates of glucose consumption and product formation were measured as a function of osmotic strength (610, 305, and 167 mOsm kg-1) and pO2 (95, 10, and 0% O2) in the presence of 5% CO2. Very mild hypoxia dramatically altered flux through the pathways of intermediary metabolism and increased the rates of production of the major metabolites, thus confirming the presence of a low-affinity O2 sensor which was active under all osmolalities tested. The data also require that as pO2 is lowered towards anoxia an endogenous carbohydrate source is mobilized. Under aerobic conditions, acute hypo-osmotic stress had little effect on product formation, whereas acute hyperosmotic stress altered metabolism in a manner similar to mild hypoxia, with the exception of decreasing the rates of acetate and succinate production. It was also shown in L. donovani promastigotes that the effects of anoxia and hyperosmolality were not additive. Thus, separate sensors with partially overlapping actions are involved in the metabolic responses to hypoxia and hyperosmolality. There was no apparent species-specificity for the responses to pO2 and osmotic stress. Uncoupling with carbonyl cyanide p-trifluoromethoxyphenylhydrazone caused changes in metabolite flux patterns which differed from the changes caused by either hypoxia or acute osmotic stress, while rotenone and calcium ionophore A23187 had no significant effects. The identity of the sensors responsive to pO2 and osmolality, and the mechanisms by which they regulate flux through the pathways of intermediary metabolism, require further study.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Walsh MJ,Blum JJ

doi

10.1016/0166-6851(92)90217-8

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

205-14

issue

2

eissn

0166-6851

issn

1872-9428

pii

0166-6851(92)90217-8

journal_volume

50

pub_type

杂志文章

相关文献

MOLECULAR AND BIOCHEMICAL PARASITOLOGY文献大全
  • TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei.

    abstract：:The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contras...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2007.06.009

    authors： Price HP,Stark M,Smith B,Smith DF

    更新日期：2007-10-01 00:00:00

  • Structure and function of Per-ARNT-Sim domains and their possible role in the life-cycle biology of Trypanosoma cruzi.

    abstract：:Per-ARNT-Sim (PAS) domains of proteins play important roles as modules for signalling and cellular regulation processes in widely diverse organisms such as Archaea, Bacteria, protists, plants, yeasts, insects and vertebrates. These domains are present in many proteins where they are used as sensors of stimuli and modu...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molbiopara.2017.11.002

    authors： Rojas-Pirela M,Rigden DJ,Michels PA,Cáceres AJ,Concepción JL,Quiñones W

    更新日期：2018-01-01 00:00:00

  • Developmental expression of high molecular weight tropomyosin isoforms in Mesocestoides corti.

    abstract：:Tropomyosins are a family of actin-binding proteins with diverse roles in actin filament function. One of the best characterized roles is the regulation of muscle contraction. Tropomyosin isoforms can be generated from different genes, and from alternative promoters and alternative splicing from the same gene. In this...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2010.11.009

    authors： Koziol U,Costábile A,Domínguez MF,Iriarte A,Alvite G,Kun A,Castillo E

    更新日期：2011-02-01 00:00:00

  • Characterisation of a simple, highly repetitive DNA sequence from the parasite Leishmania donovani.

    abstract：:Repetitive DNA sequences of the Leishmania donovani genome have been identified by screening a recombinant DNA library made by cloning sheared genomic DNA into the vector pAT153. Bacterial clones containing a highly repetitive DNA sequence have been isolated. DNA sequencing has shown that this sequence is composed of ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(88)90114-4

    authors： Ellis J,Crampton J

    更新日期：1988-05-01 00:00:00

  • The effect of over-expression of the alternative oxidase in the procyclic forms of Trypanosoma brucei.

    abstract：:Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2004.11.003

    authors： Walker R Jr,Saha L,Hill GC,Chaudhuri M

    更新日期：2005-02-01 00:00:00

  • Nuclear actin-related protein is required for chromosome segregation in Toxoplasma gondii.

    abstract：:Apicomplexa parasites use complex cell cycles to replicate that are not well understood mechanistically. We have established a robust forward genetic strategy to identify the essential components of parasite cell division. Here we describe a novel temperature sensitive Toxoplasma strain, mutant 13-20C2, which growth a...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2011.09.006

    authors： Suvorova ES,Lehmann MM,Kratzer S,White MW

    更新日期：2012-01-01 00:00:00

  • Molecular interactions of Leishmania promastigote surface protease with human alpha 2-macroglobulin.

    abstract：:The interaction of Leishmania promastigote surface protease (PSP) with the plasmatic protease inhibitor alpha 2-macroglobulin (alpha 2M) was investigated. In plasma, solubilized PSP forms covalent complexes only with alpha 2M, at the exclusion of other protease inhibitors. The formation of complexes is accompanied by ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(89)90043-1

    authors： Heumann D,Burger D,Vischer T,de Colmenares M,Bouvier J,Bordier C

    更新日期：1989-02-01 00:00:00

  • Detection, purification and partial characterization of beta-amylase from trophozoites of Entamoeba invadens.

    abstract：:Trophozoites of Entamoeba invadens were able to ingest glucopolysaccharides and metabolize them. An activity capable of degrading these substrates was purified to apparent homogeneity. The enzyme was identified as beta-amylase (alpha-1,4-D-glucan maltohydrolase EC 3.2.1.2): It was active against glycogen, amylose and ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(96)02765-x

    authors： Serafimova F,Franz A,Werries E

    更新日期：1996-12-20 00:00:00

  • Peroxisomes in parasitic protists.

    abstract：:Representatives of all major lineages of eukaryotes contain peroxisomes with similar morphology and mode of biogenesis, indicating a monophyletic origin of the organelles within the common ancestor of all eukaryotes. Peroxisomes originated from the endoplasmic reticulum, but despite a common origin and shared morpholo...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molbiopara.2016.02.005

    authors： Gabaldón T,Ginger ML,Michels PA

    更新日期：2016-01-01 00:00:00

  • L-myo-Inositol 1-phosphate synthase from Entamoeba histolytica.

    abstract：:L-myo-Inositol 1-phosphate synthase (I-1-P synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. Inositol is a precursor of essential macromolecules like membrane phospholipids, GPI anchor proteins and lipophos...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(98)00147-9

    authors： Lohia A,Hait NC,Majumder AL

    更新日期：1999-01-05 00:00:00

  • Conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, in field isolates and distinct species of Plasmodium.

    abstract：:The extent of structural conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, recently characterized in the T9/96 clone, has been analyzed using the polymerase chain reaction. Results from Ivory Coast and Thai clones, field isolates originating from Brazil and Kenya and laboratory-maintain...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)00138-3

    authors： Fidock DA,Sallenave-Sales S,Sherwood JA,Gachihi GS,Ferreira-da-Cruz MF,Thomas AW,Druilhe P

    更新日期：1994-10-01 00:00:00

  • Alpha and beta subunits of pyruvate dehydrogenase E1 from the microsporidian Nosema locustae: mitochondrion-derived carbon metabolism in microsporidia.

    abstract：:Microsporidia are highly adapted eukaryotic intracellular parasites that infect a variety of animals. Microsporidia contain no recognisable mitochondrion, but recently have been shown to have evolved from fungi and to possess heat shock protein genes derived from mitochondria. These findings make it clear that microsp...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(01)00356-5

    authors： Fast NM,Keeling PJ

    更新日期：2001-10-01 00:00:00

  • Proteolytic activity of Plasmodium falciparum subtilisin-like protease 3 on parasite profilin, a multifunctional protein.

    abstract：:Subtilisin-like proteases of malaria parasite Plasmodium falciparum (PfSUB1, 2 and 3) are expressed at late asexual blood stages. PfSUB1 and 2 are considered important drug targets due to their essentiality for parasite blood stages and role in merozoite egress and invasion of erythrocytes. We have earlier shown the i...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2013.09.006

    authors： Alam A,Bhatnagar RK,Relan U,Mukherjee P,Chauhan VS

    更新日期：2013-10-01 00:00:00

  • De novo arachidonic acid synthesis in Perkinsus marinus, a protozoan parasite of the eastern oyster Crassostrea virginica.

    abstract：:The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 13C-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 13C-acetate and palmitic-d(31) acid were analyzed...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(01)00413-3

    authors： Chu FL,Lund E,Soudant P,Harvey E

    更新日期：2002-02-01 00:00:00

  • Molecules on the surface of the Plasmodium falciparum infected erythrocyte and their role in malaria pathogenesis and immune evasion.

    abstract：:The surface of the erythrocyte undergoes a number of modifications during infection by Plasmodium falciparum. These modifications are critical for pathogenesis of severe disease and the acquisition of host immunity through their role in interactions between the host and the parasite and in antigenic variation. Our kno...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章,评审

    doi：10.1016/s0166-6851(01)00275-4

    authors： Craig A,Scherf A

    更新日期：2001-07-01 00:00:00

  • Chemical modification of fructose bisphosphate aldolase from Trypanosoma brucei compared to aldolase from rabbit muscle and Staphylococcus aureus.

    abstract：:Chemical modifications of Class I aldolases from Trypanosoma brucei, rabbit muscle and Staphylococcus aureus with carboxypeptidase A, glyceraldehyde 3-phosphate and cysteine-specific reagents revealed the following differences between the three homologous enzymes. Aldolase from S. aureus was not affected by any of the...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90143-t

    authors： Callens M,Opperdoes FR

    更新日期：1991-07-01 00:00:00

  • Hepatic mixed-function oxidase activity in mice infected with Trypanosoma brucei gambiense or treated with trypanocides.

    abstract：:Three days after mice were inoculated with Trypanosoma brucei gambiense, total hepatic cytochrome P-450 levels were decreased 14% from control values, while the specific mixed-function oxidase activity (per nmole of cytochrome P-450) was inhibited almost 40% in infected animals. Furthermore, drugs used to treat trypan...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(81)90051-7

    authors： Shertzer HG,Hall JE,Seed JR

    更新日期：1981-08-01 00:00:00

  • Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences.

    abstract：:Polymerase chain reaction followed by high resolution melting (PCR-HRM) analysis is a simple, rapid and accurate method for molecular detection of various nematode species. The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2020.111260

    authors： Arbabi M,Hooshyar H,Lotfinia M,Bakhshi MA

    更新日期：2020-03-01 00:00:00

  • Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach.

    abstract：:Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a jo...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2021.111350

    authors： Stevanovic S,Marjanović DS,Trailović SM,Zdravković N,Perdih A,Nikolic K

    更新日期：2021-01-07 00:00:00

  • Transient expression of luciferase in Entamoeba histolytica driven by the ferredoxin gene 5' and 3' regions.

    abstract：:We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(95)02474-3

    authors： Gilchrist CA,Streets HL,Ackers JP,Hall R

    更新日期：1995-10-01 00:00:00

  • The Haemonchus contortus UNC-49B subunit possesses the residues required for GABA sensitivity in homomeric and heteromeric channels.

    abstract：:Hco-UNC-49 is a GABA receptor from the parasitic nematode Haemonchus contortus that has a relatively low overall sequence similarity to vertebrate GABA receptors but is very similar to the UNC-49 receptor found in the free living nematode Caenorhabditis elegans. While the nematode receptors do share >80% sequence simi...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2011.04.002

    authors： Accardi MV,Forrester SG

    更新日期：2011-07-01 00:00:00

  • Trypanosoma brucei MRE11 is non-essential but influences growth, homologous recombination and DNA double-strand break repair.

    abstract：:MRE11 is a conserved multi-functional protein that is important for maintaining genomic integrity in yeast and mammalian cells. By database searching, we identified a full-length candidate MRE11 on Trypanosoma brucei chromosome II. We subsequently cloned and sequenced the corresponding gene from the Lister 427 strain....

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(02)00165-2

    authors： Tan KS,Leal ST,Cross GA

    更新日期：2002-11-01 00:00:00

  • The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD.

    abstract：:The glycoproteins of 12-28 kD from Taenia solium metacestodes provide a high specificity and sensitivity for the serological diagnosis of the central nervous system infection, neurocysticercosis. Their widespread use as antigens for routine serological assays will require their production in large and reproducible amo...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(01)00256-0

    authors： Obregón-Henao A,Gil DL,Gómez DI,Sanzón F,Teale JM,Restrepo BI

    更新日期：2001-05-01 00:00:00

  • Occurrence and transfection of a Giardia virus.

    abstract：:The presence of virus-derived RNA was investigated in 38 axenically growing Giardia isolates from different geographic areas. The RNA virus was demonstrated in Giardia strains from humans in the U.S.A., England and the majority of strains from Poland. Two strains isolated respectively from a cat and a cavia also conta...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(87)90190-3

    authors： De Jonckheere JF,Gordts B

    更新日期：1987-02-01 00:00:00

  • Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei.

    abstract：:Trypanosoma brucei harbors a unique multifunctional RNA polymerase (pol) I which transcribes, in addition to ribosomal RNA genes, the gene units encoding the major cell surface antigens variant surface glycoprotein and procyclin. In consequence, this RNA pol I is recruited to three structurally different types of prom...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2006.02.023

    authors： Nguyen TN,Schimanski B,Zahn A,Klumpp B,Günzl A

    更新日期：2006-09-01 00:00:00

  • 3-Methyladenine blocks Toxoplasma gondii division prior to centrosome replication.

    abstract：:The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2010.05.020

    authors： Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

    更新日期：2010-10-01 00:00:00

  • Molecular cloning, organellar targeting and developmental expression of mitochondrial chaperone HSP60 in Toxoplasma gondii.

    abstract：:The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(00)00324-8

    authors： Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

    更新日期：2000-12-01 00:00:00

  • The merozoite surface protein 6 gene codes for a 36 kDa protein associated with the Plasmodium falciparum merozoite surface protein-1 complex.

    abstract：:A complex of non-covalently bound polypeptides is located on the surface of the merozoite form of the human malaria parasite Plasmodium falciparum. Four of these polypeptides are derived by proteolytic processing of the merozoite surface protein 1 (MSP-1) precursor. Two components, a 22 and a 36 kDa polypeptide are no...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(00)00350-9

    authors： Trucco C,Fernandez-Reyes D,Howell S,Stafford WH,Scott-Finnigan TJ,Grainger M,Ogun SA,Taylor WR,Holder AA

    更新日期：2001-01-15 00:00:00

  • Immunoprecipitation of surface antigen precursors from Schistosoma mansoni messenger RNA in vitro translation products.

    abstract：:Messenger RNA has been isolated from adults and eggs of Schistosoma mansoni and translated in vitro in mRNA dependent rabbit reticulocyte lysate. The in vitro translation products have been immunoprecipitated using a wide variety of hyperimmune and infection sera from rabbits, mice and humans. A large number of in vit...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(84)90029-x

    authors： Taylor DW,Cordingley JS,Butterworth AE

    更新日期：1984-03-01 00:00:00

  • Molecular basis of binding of the Plasmodium falciparum receptor BAEBL to erythrocyte receptor glycophorin C.

    abstract：:Plasmodium falciparum invades human erythrocytes by redundant pathways. Unlike Plasmodium vivax that has one Duffy Binding-Like (DBL) receptor, P. falciparum has four members of the DBL receptor family. Furthermore, one of these DBL genes, BAEBL, has polymorphisms at four amino acids in region II; each polymorphism bi...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2009.06.006

    authors： Jiang L,Duriseti S,Sun P,Miller LH

    更新日期：2009-11-01 00:00:00