Characterisation of a simple, highly repetitive DNA sequence from the parasite Leishmania donovani.


:Repetitive DNA sequences of the Leishmania donovani genome have been identified by screening a recombinant DNA library made by cloning sheared genomic DNA into the vector pAT153. Bacterial clones containing a highly repetitive DNA sequence have been isolated. DNA sequencing has shown that this sequence is composed of tandem repeats of the sequence 5'-CCCTAA-3'. This sequence is identical to the telomeric repeats found in Trypanosoma brucei and hybridizes to all Leishmania chromosomes. In this study we show that there is considerable heterogeneity in the distribution and copy number of this repeat and associated hybridising sequences throughout the genomes of different Leishmania species.


Mol Biochem Parasitol


Ellis J,Crampton J




Has Abstract


1988-05-01 00:00:00














  • Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei.

    abstract::Trypanosoma brucei harbors a unique multifunctional RNA polymerase (pol) I which transcribes, in addition to ribosomal RNA genes, the gene units encoding the major cell surface antigens variant surface glycoprotein and procyclin. In consequence, this RNA pol I is recruited to three structurally different types of prom...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nguyen TN,Schimanski B,Zahn A,Klumpp B,Günzl A

    更新日期:2006-09-01 00:00:00

  • Characterization and localization of Plasmodium falciparum homolog of prokaryotic ClpQ/HslV protease.

    abstract::The beta subunits (beta1, beta2, and beta5) of 20S proteasome and HslV/ClpQ are ATP-dependent threonine proteases present in eukaryotes and prokaryotes, respectively that control levels of key regulatory proteins in the cell. The orthologue of prokaryotic HslV protease in Plasmodium falciparum (PfHslV) is a novel drug...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ramasamy G,Gupta D,Mohmmed A,Chauhan VS

    更新日期:2007-04-01 00:00:00

  • Characterisation of the novel junctions of two minichromosomes of Leishmania major.

    abstract::The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ortiz G,Segovia M

    更新日期:1996-11-25 00:00:00

  • Sialic acid-dependent binding of baculovirus-expressed recombinant antigens from Plasmodium falciparum EBA-175 to Glycophorin A.

    abstract::The Plasmodium falciparum Erythrocyte Binding Antigen-175, EBA-175, is a soluble merozoite stage parasite protein which binds to glycophorin A surface receptors on human erythrocytes. We have expressed two conserved cysteine-rich regions, region II and region VI, of this protein as soluble His-tagged polypeptides in i...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ockenhouse CF,Barbosa A,Blackall DP,Murphy CI,Kashala O,Dutta S,Lanar DE,Daugherty JR

    更新日期:2001-03-01 00:00:00

  • RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs.

    abstract::Schistosoma mansoni leucine aminopeptidase (LAP) is thought to play a central role in hatching of the miracidium from the schistosome egg. We identified two discrete LAPs genes in the S. mansoni genome, and their orthologs in S. japonicum. The similarities in sequence and exon/intron structure of the two genes, LAP1 a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rinaldi G,Morales ME,Alrefaei YN,Cancela M,Castillo E,Dalton JP,Tort JF,Brindley PJ

    更新日期:2009-10-01 00:00:00

  • Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum: different logics achieve the same goal.

    abstract::Babesia bovis is a protozoal hemoparasite of cattle which behaves in certain crucial respects like Plasmodium falciparum, despite being phylogenetically distant and having many differences in its life cycle. The shared behavioral attributes of rapid antigenic variation and cytoadhesion/sequestration are thought to con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Allred DR,Al-Khedery B

    更新日期:2004-03-01 00:00:00

  • Identification by extrachromosomal amplification and overexpression of a zeta-crystallin/NADPH-oxidoreductase homologue constitutively expressed in Leishmania spp.

    abstract::A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liu X,Chang KP

    更新日期:1994-08-01 00:00:00

  • Characterization of acyl carrier protein and LytB in Babesia bovis apicoplast.

    abstract::The apicoplast is a highly specialized organelle that mediates required functions in the growth and replication of apicomplexan parasites. Despite structural conservation of the apicoplast among different parasite genera and species, there are also critical differences in the metabolic requirements of different parasi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Caballero MC,Pedroni MJ,Palmer GH,Suarez CE,Davitt C,Lau AO

    更新日期:2012-02-01 00:00:00

  • Identification of immunoreactive proteins from the dog heartworm (Dirofilaria immitis) differentially recognized by the sera from dogs with patent or occult infections.

    abstract::Heartworm disease caused by Dirofilaria immitis affects canine and feline hosts. Moreover, the parasite can infect humans, causing pulmonary dirofilariosis. Most affected dogs have patent infections with circulating microfilariae in peripheral blood, although infected dogs sometimes develop occult infections character...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Oleaga A,Pérez-Sánchez R,Pagés E,Marcos-Atxutegi C,Simón F

    更新日期:2009-08-01 00:00:00

  • Molecular cloning, organellar targeting and developmental expression of mitochondrial chaperone HSP60 in Toxoplasma gondii.

    abstract::The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

    更新日期:2000-12-01 00:00:00

  • Sequences with high propensity to form G-quartet structures in kinetoplast DNA from Phytomonas serpens.

    abstract::Naturally occurring sequences containing repetitive guanine motifs have the potential to form tetraplex DNA. Phytomonas serpens minicircle DNA shows some regions where one strand is composed mainly of G and T (GT regions). These regions contain several stretches of contiguous guanines. An oligonucleotide was construct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sá-Carvalho D,Traub-Cseko YM

    更新日期:1995-06-01 00:00:00

  • Molecular cloning, genomic structure and localization in a blood stage antigen of Plasmodium falciparum characterized by a serine stretch.

    abstract::Two short DNA segments were isolated by screening of a lambda gt11 library from Plasmodium falciparum schizont cDNA with an antiserum against the 140 kDa protein, which confers protective immunity to monkeys. The segments were used to identify a genomic fragment which carries the entire coding sequence for a protein o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Knapp B,Hundt E,Nau U,Küpper HA

    更新日期:1989-01-01 00:00:00

  • Molecular cloning and analysis of Ancylostoma ceylanicum glutamate-cysteine ligase.

    abstract::Glutamate-cysteine ligase (GCL) is a heterodimer enzyme composed of a catalytic subunit (GCLC) and a modifier subunit (GCLM). This enzyme catalyses the synthesis of γ-glutamylcysteine, a precursor of glutathione. cDNAs of the putative glutamate-cysteine ligase catalytic (Ace-GCLC) and modifier subunits (Ace-GCLM) of A...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wiśniewski M,Lapiński M,Zdziarska A,Długosz E,Bąska P

    更新日期:2014-08-01 00:00:00

  • Gender biased differential alternative splicing patterns of the transcriptional cofactor CA150 gene in Schistosoma mansoni.

    abstract::The complex molecular systems involved in the process of sex-differentiation and fertility in Schistosoma mansoni have not yet been completely described. Using a 4608-element cDNA microarray, we have now determined 90 and 139 genes with significantly (q-value

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: DeMarco R,Oliveira KC,Venancio TM,Verjovski-Almeida S

    更新日期:2006-12-01 00:00:00

  • Gene disruption reveals a dispensable role for plasmepsin VII in the Plasmodium berghei life cycle.

    abstract::Plasmepsins (PM), aspartic proteases of Plasmodium, comprises a family of ten proteins that perform critical functions in Plasmodium life cycle. Except VII and VIII, functions of the remaining plasmepsin members have been well characterized. Here, we have generated a mutant parasite lacking PM VII in Plasmodium berghe...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mastan BS,Kumari A,Gupta D,Mishra S,Kumar KA

    更新日期:2014-06-01 00:00:00

  • Involvement of lysosomes in the uptake of macromolecular material by bloodstream forms of Trypanosoma brucei.

    abstract::To investigate whether the lysosomes of Trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used Triton WR-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. In...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Opperdoes FR,Van Roy J

    更新日期:1982-09-01 00:00:00

  • Biochemical and genetic analysis of the distinct proliferating cell nuclear antigens of Toxoplasma gondii.

    abstract::The apicomplexa parasite Toxoplasma gondii expresses two distinct proliferating cell nuclear antigens (PCNA) that exhibit distinct patterns of subcellular localization during tachyzoite growth. In all cell cycle phases, TgPCNA1 is concentrated in the nucleus, while TgPCNA2 is only concentrated in the nucleus during S-...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Guerini MN,Behnke MS,White MW

    更新日期:2005-07-01 00:00:00

  • A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes.

    abstract::During blood-stage infection, malaria parasites export numerous proteins to the host erythrocyte. The Poly-Helical Interspersed Sub-Telomeric (PHIST) proteins are an exported family that share a common 'PRESAN' domain, and include numerous members in Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi. In ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tarr SJ,Moon RW,Hardege I,Osborne AR

    更新日期:2014-08-01 00:00:00

  • Structural features affecting variant surface glycoprotein expression in Trypanosoma brucei.

    abstract::The glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) of Trypanosoma brucei is the most abundant GPI-anchored protein expressed on any cell, and is an essential virulence factor. To determine what structural features affect efficient expression of VSG, we made a series of mutations in two ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang J,Böhme U,Cross GA

    更新日期:2003-05-01 00:00:00

  • Leupeptin alters the proteolytic processing of P126, the major parasitophorous vacuole antigen of Plasmodium falciparum.

    abstract::Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 7...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Debrabant A,Delplace P

    更新日期:1989-03-01 00:00:00

  • Biochemical characterization of Toxoplasma gondii 1-Cys peroxiredoxin 2 with mechanistic similarities to typical 2-Cys Prx.

    abstract::TgPrx2 represents a recently discovered cytosolic 1-Cys peroxiredoxin (Prx) from the intracellular parasite Toxoplasma gondii. Over-expression of the respective gene confers protection against H(2)O(2), suggesting that the protein possesses peroxidase activity. According to the current nomenclature eukaryotic typical ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Deponte M,Becker K

    更新日期:2005-03-01 00:00:00

  • DNA sequence of Crithidia fasciculata kinetoplast minicircles.

    abstract::Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sugisaki H,Ray DS

    更新日期:1987-04-01 00:00:00

  • The secreted and somatic antigens of the third stage larva of Anisakis simplex, and antigenic relationship with Ascaris suum, Ascaris lumbricoides, and Toxocara canis.

    abstract::The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

    更新日期:1988-10-01 00:00:00

  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • Stable transfection and continuous expression of heterologous genes in Entamoeba invadens.

    abstract::Amoebiasis is spread by the ingestion of dormant Entamoeba histolytica cysts. Intervention of encystation could break the transmission cycle, thereby reducing disease burden. The model system used to study trophozoite to cyst differentiation is Entamoeba invadens. Here we describe an electroporation-based method for s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Singh N,Ojha S,Bhattacharya A,Bhattacharya S

    更新日期:2012-07-01 00:00:00

  • Characterization of novel fucosyl- and tyvelosyl-containing glycoconjugates from Trichinella spiralis muscle stage larvae.

    abstract::The monosaccharide composition of an affinity-purified family of antigenically-related Trichinella spiralis larval glycoproteins was determined by gas chromatography/mass spectrometry. This group of 6 major glycoproteins, designated TSL-1, originates in the muscle stage (L1) larval stichosome. They are present on the ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wisnewski N,McNeil M,Grieve RB,Wassom DL

    更新日期:1993-09-01 00:00:00

  • A short interspersed repetitive element provides a new 3' acceptor site for trans-splicing in certain ribosomal P2 beta protein genes of Trypanosoma cruzi.

    abstract::Four Trypanosoma cruzi genomic DNA fragments carrying different TcP2 beta genes have been isolated and sequenced. Three of them had a single TcP2 beta gene, while the 3.8-kb-long DNA segment encoding the TcP2 beta-H1.8 locus showed two TcP2 beta genes arranged in tandem. These genes were physically connected by a 428-...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Vazquez MP,Schijman AG,Levin MJ

    更新日期:1994-04-01 00:00:00

  • Divergent evolutionary constraints on mitochondrial and nuclear genomes of malaria parasites.

    abstract::Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falcip...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: McIntosh MT,Srivastava R,Vaidya AB

    更新日期:1998-09-01 00:00:00

  • Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.

    abstract::The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the struct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chen M,Li E,Stanley SL Jr

    更新日期:2004-10-01 00:00:00

  • The Leishmania promastigote surface antigen 2 complex is differentially expressed during the parasite life cycle.

    abstract::The promastigote surface antigen 2 (PSA-2) complex comprises a family of antigenically similar polypeptides of M(r) 96,000, 80,000 and 50,000, anchored to the membrane with glycosylphosphatidylinositol. Although PSA-2 was initially detected only in promastigotes, Northern blot analysis indicated that mRNA transcripts ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Osborn AH,Symons F,van Driel R,Cappai R

    更新日期:1995-11-01 00:00:00