Abstract：
:Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the complex to be shed and leaving only a small 15kDa sub-unit, MSP-1(19), on the merozite surface. MSP-1(19) is considered a strong vaccine candidate. We have determined the solution structure of MSP-1(19) from P. vivax using nuclear magnetic resonance (NMR) and show that, like in other Plasmodium species, it consists of two EGF-like domains that are oriented head-to-tail. The protein has a flat, disk-like shape with a highly charged surface. When MSP-1(19) is part of the larger MSP-1(42) precursor it exists as an independent domain with no stable contacts to the rest of the sub-unit. Gel filtration and analytical ultracentrifugation experiments indicate that P. vivax MSP-1(42) exists as a dimer in solution. MSP-1(19) itself is a monomer, however, 35 amino-acids immediately upstream of its N-terminus are sufficient to cause dimerization. Our data suggest that if MSP-1(42) exists as a dimer in vivo, secondary processing would cause the dissociation of two tightly linked MSP-1(19) proteins on the merozoite surface just prior to invasion.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Babon JJ,Morgan WD,Kelly G,Eccleston JF,Feeney J,Holder AAdoi
10.1016/j.molbiopara.2007.01.015subject
Has Abstractpub_date
2007-05-01 00:00:00pages
31-40issue
1eissn
0166-6851issn
1872-9428pii
S0166-6851(07)00041-2journal_volume
153pub_type
杂志文章abstract：:The aim of this study was to investigate the occurrence, distribution and immunochemical properties of antibody-defined carbohydrate epitopes in neutral glycolipid fractions of Schistosoma mansoni eggs, cercariae and adults. The amount of extractable, antigenic, neutral glycolipids was lowest in adult worms, increasin...
journal_title：Molecular and biochemical parasitology
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更新日期：2003-07-01 00:00:00
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更新日期：2007-01-01 00:00:00
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journal_title：Molecular and biochemical parasitology
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pub_type： 杂志文章
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更新日期：1994-12-01 00:00:00
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pub_type： 杂志文章
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