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Isolation and substrate specificity of an adenine nucleoside phosphorylase from adult Schistosoma mansoni.

Abstract：

:An adenine nucleoside phosphorylase (ANP, EC none) activity was identified and partially purified from extracts of Schistosoma mansoni by chromatofocussing column chromatography and molecular sieving. The enzyme is distinct from purine nucleoside phosphorylase (PNP, EC 2.4.2.1). ANP is specific for adenine nucleosides which includes adenosine analogs modified in the aglycone, pentose or both moieties. (e.g. 2'-deoxyadenosine, 5'-deoxy-5'-methylthioadenosine, 5'-deoxy-5'-iodo-2-fluoroadenosine, etc.) The enzyme is also distinct from the mammalian 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP, EC 2.4.2.28) in that it is able of the phosphorolysis of 2'-deoxyadenosine while mammalian MTAP cannot. Because of ANP unique substrate specificity, the enzyme could play a role as a target for chemotherapy of these parasites. Cytotoxic analogs may be designed as subversive substrates that are selectively activated only by the schistosomal ANP.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Savarese TM,El Kouni MH

doi

10.1016/j.molbiopara.2014.04.008

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

44-7

issue

1-2

eissn

0166-6851

issn

1872-9428

pii

S0166-6851(14)00056-5

journal_volume

194

pub_type

杂志文章

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