Identification of the Echinococcus (hydatid disease) organisms using cloned DNA markers.


:Cloned DNA fragments of the ribosomal RNA gene of Schistosoma mansoni hybridise strongly to Echinococcus DNA following restriction endonuclease and Southern transfer analysis. Individuals within a strain of E. granulosus exhibit identical patterns of hybridisation. However, the hybridisation patterns show significant differences between E. granulosus and E. multilocularis, and between the horse and sheep strains of E. granulosus. This technique represents a powerful, additional method for the identification and characterisation of new isolates of E. granulosus and E. multilocularis.


Mol Biochem Parasitol


McManus DP,Simpson AJ




Has Abstract


1985-11-01 00:00:00














  • Characterization of Leishmania major phosphatidylethanolamine methyltransferases LmjPEM1 and LmjPEM2 and their inhibition by choline analogs.

    abstract::Phosphatidylcholine (PC) is the most abundant phospholipid in the membranes of the human parasite Leishmania. It is synthesized via two metabolic routes, the de novo pathway that starts with the uptake of choline, and the threefold methylation of phosphatidylethanolamine. Choline was shown to be dispensable for Leishm...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bibis SS,Dahlstrom K,Zhu T,Zufferey R

    更新日期:2014-09-01 00:00:00

  • A gene-family encoding small exported proteins is conserved across Plasmodium genus.

    abstract::A gene-family, named sep, encoding small exported proteins conserved across Plasmodium species has been identified. SEP proteins (13-16 kDa) contain a predicted signal peptide at the NH(2)-terminus, an internal hydrophobic region and a polymorphic, low-complexity region at the carboxy-terminus. One member of the Plasm...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

    更新日期:2003-02-01 00:00:00

  • Cloning and sequencing of an hsp70 gene of Schistosoma mansoni.

    abstract::Schistosomes have a complex life cycle (vertebrate and molluscan hosts as well as larvae living freely in water) in which they are exposed to different environments and temperatures (20 degrees C - 37 degrees C). Since heat shock genes are activated in response to stress and during development [1], it is of interest t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Neumann S,Ziv E,Lantner F,Schechter I

    更新日期:1992-12-01 00:00:00

  • Leupeptin alters the proteolytic processing of P126, the major parasitophorous vacuole antigen of Plasmodium falciparum.

    abstract::Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 7...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Debrabant A,Delplace P

    更新日期:1989-03-01 00:00:00

  • The ecdysone receptor (EcR) is a major regulator of tissue development and growth in the marine salmonid ectoparasite, Lepeophtheirus salmonis (Copepoda, Caligidae).

    abstract::The function of the ecdysone receptor (EcR) during development and molting has been thoroughly investigated in some arthropods such as insects but rarely in crustacean copepods such as the salmon louse Lepeophtheirus salmonis (L. salmonis) (Copepoda, Caligidae). The salmon louse is an ectoparasite on Atlantic salmon t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sandlund L,Nilsen F,Male R,Dalvin S

    更新日期:2016-08-01 00:00:00

  • L-myo-Inositol 1-phosphate synthase from Entamoeba histolytica.

    abstract::L-myo-Inositol 1-phosphate synthase (I-1-P synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. Inositol is a precursor of essential macromolecules like membrane phospholipids, GPI anchor proteins and lipophos...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lohia A,Hait NC,Majumder AL

    更新日期:1999-01-05 00:00:00

  • Expression and functional characterization of a giant Type I fatty acid synthase (CpFAS1) gene from Cryptosporidium parvum.

    abstract::A 25-kb CpFAS1 gene from Cryptosporidium parvum has been engineered and expressed as five individual maltose-binding protein (MBP)-fusion proteins: an N-terminal loading unit, three fatty acyl elongation modules, and a C-terminal reductase. Enzymatic activities of all domains (except the reductase) were individually a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zhu G,Li Y,Cai X,Millership JJ,Marchewka MJ,Keithly JS

    更新日期:2004-03-01 00:00:00

  • A tubulin-related 55 kilodalton surface antigen recognized by different Trypanosoma cruzi stage-specific monoclonal antibodies from infected mice.

    abstract::Thirteen monoclonal antibodies (MAbs) specific for the membrane of live Trypanosoma cruzi have been obtained from BALB/c infected mice. Most of them had greater avidity for intact than for disrupted parasites. According to the staining by indirect immunofluorescence of the different live developmental stages of the pa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alcina A,Fresno M

    更新日期:1988-06-01 00:00:00

  • Interallelic recombination in the merozoite surface protein 1 (MSP-1) gene of Plasmodium vivax from Thai isolates.

    abstract::The merozoite of Plasmodium vivax possesses a high molecular mass surface protein called Pv-merozoite surface protein 1, PvMSP-1, which exhibits antigenic diversity among isolates. In this study, the extent of sequence variation in the polymorphic region and the flanking interspecies conserved blocks (ICBs) 5 and 6 of...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Putaporntip C,Jongwutiwes S,Tanabe K,Thaithong S

    更新日期:1997-01-01 00:00:00

  • Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins.

    abstract::The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ismaïli N,Pérez-Morga D,Walsh P,Mayeda A,Pays A,Tebabi P,Krainer AR,Pays E

    更新日期:1999-07-30 00:00:00

  • Predicted structure of a major Schistosoma mansoni eggshell protein.

    abstract::The complete sequence for a major Schistosome mansoni eggshell protein gene has been determined from a genomic DNA fragment. The use of an open reading frame encoding a glycine-rich polypeptide was confirmed by in vitro translation of schistosome mRNA in the presence of [3H]glycine and comparison with the amino acid c...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rodrigues V,Chaudhri M,Knight M,Meadows H,Chambers AE,Taylor WR,Kelly C,Simpson AJ

    更新日期:1989-01-01 00:00:00

  • Upstream regulatory elements controlling expression of the Entamoeba histolytica lectin.

    abstract::Entamoeba histolytica genomic organization and putative promoter elements appear to be distinct from both metazoan and better characterized protozoan organisms. The recent development of DNA-mediated transfection for E. histolytica enabled characterization of cis-acting promoter elements required for gene expression. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Purdy JE,Pho LT,Mann BJ,Petri WA Jr

    更新日期:1996-06-01 00:00:00

  • The Kennedy phospholipid biosynthesis pathways are refractory to genetic disruption in Plasmodium berghei and therefore appear essential in blood stages.

    abstract::Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) are the main membrane phospholipids (PLs) of Plasmodium parasites and can be generated by the de novo (Kennedy) CDP-choline and CDP-ethanolamine pathways and by the CDP-diacylglycerol dependent pathway. The Kennedy pathways initiate from exogenous choline and ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Déchamps S,Wengelnik K,Berry-Sterkers L,Cerdan R,Vial HJ,Gannoun-Zaki L

    更新日期:2010-10-01 00:00:00

  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei.

    abstract::Trypanosoma brucei harbors a unique multifunctional RNA polymerase (pol) I which transcribes, in addition to ribosomal RNA genes, the gene units encoding the major cell surface antigens variant surface glycoprotein and procyclin. In consequence, this RNA pol I is recruited to three structurally different types of prom...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nguyen TN,Schimanski B,Zahn A,Klumpp B,Günzl A

    更新日期:2006-09-01 00:00:00

  • Purification and characterization of gamma-glutamyl transpeptidase from Ascaris suum.

    abstract::gamma-Glutamyl transpeptidase, which is of central importance in the degradation of glutathione, was purified from Ascaris suum to apparent homogeneity. The enzyme was found to be a predominantly membrane-bound protein and was solubilized by Triton X-100. The purified enzyme, which exhibits a specific activity of 1009...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hussein AS,Walter RD

    更新日期:1996-04-01 00:00:00

  • Characterisation of the novel junctions of two minichromosomes of Leishmania major.

    abstract::The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ortiz G,Segovia M

    更新日期:1996-11-25 00:00:00

  • Development of an untargeted metabolomics method for the analysis of human faecal samples using Cryptosporidium-infected samples.

    abstract::Faecal metabolite profiling, though in its infancy, allows for investigation of complex metabolic interactions between gastrointestinal infections or diseases and host health. In the present study, we describe a faecal metabolite extraction method for untargeted gas chromatography-mass spectrometry (GC-MS) analysis us...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ng JS,Ryan U,Trengove RD,Maker GL

    更新日期:2012-10-01 00:00:00

  • A Trypanosoma brucei mutant resistant to alpha-difluoromethylornithine.

    abstract::Procyclic Trypanosoma brucei brucei strain 366D is susceptible to DL-alpha-difluoromethylornithine (DFMO) with an in vitro ED50 value of 225 microM. A mutant of the procyclic strain resistant to 20 mM of DFMO was isolated by serial in vitro passages of the organisms in increasing concentrations of the drug. Drug resis...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Phillips MA,Wang CC

    更新日期:1987-01-02 00:00:00

  • Differential toxicity of ricin and diphtheria toxin for bloodstream forms of Trypanosoma brucei.

    abstract::The cytotoxicity of ricin and diphtheria toxin was studied in culture-adapted bloodstream forms of Trypanosoma brucei. Although ricin is endocytosed at a rate comparable to that of other internalized macromolecules, it is nontoxic to bloodstream-form trypanosomes. The resistance lies partly in low susceptibility of th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Scory S,Steverding D

    更新日期:1997-12-01 00:00:00

  • Structural and functional characterization of the Leishmania amazonensis ribosomal RNA promoter.

    abstract::The promoter region of the ribosomal RNA (rRNA) genes of Leishmania amazonensis was characterised and the transcription start point, defined by primer extension, was shown to be a T residue, 1048 nucleotides upstream of the beginning of the 18S sequence. A repetitive element of 60 bp was identified in the intergenic s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Uliana SR,Fischer W,Stempliuk VA,Floeter-Winter LM

    更新日期:1996-02-01 00:00:00

  • The electrochemical proton gradient in the bloodstream form of Trypanosoma brucei is dependent on the temperature.

    abstract::The membrane potential and pH gradient over the plasma membrane of the protozoan parasite Trypanosoma brucei were measured with radioactive indicators in combination with the silicone oil centrifugation technique over a range of temperatures. At 37 degrees C a small membrane potential and pH gradient of similar magnit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ter Kuile BH,Wiemer EA,Michels PA,Opperdoes FR

    更新日期:1992-10-01 00:00:00

  • Homologous telomeric sequences are present in different species of the genus Plasmodium.

    abstract::The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dore E,Pace T,Ponzi M,Scotti R,Frontali C

    更新日期:1986-11-01 00:00:00

  • Trichomonas vaginalis ribosomal RNA: identification and characterisation of the transcription promoter and terminator sequences.

    abstract::Trichomonas vaginalis is a parasitic protozoan of both medical and biological relevance. Transcriptional studies in this organism have focused mainly on type II pol promoters, whereas the elements necessary for transcription by polI or polIII have not been investigated. Here, with the aid of a transient transcription ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Franco B,Hernández R,López-Villaseñor I

    更新日期:2012-09-01 00:00:00

  • Leishmania donovani PP2C: Kinetics, structural attributes and in vitro immune response.

    abstract::Most of the signaling pathways are regulated by reversible phosphorylation-dephosphorylation which involves enzymes- kinases and phosphatases. Current knowledge about the protein phosphatases in parasites like Trypanosoma and Leishmania is very minimal despite their enormousity. In present study, full length ORF of Le...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jakkula P,Qureshi R,Iqbal A,Sagurthi SR,Qureshi IA

    更新日期:2018-07-01 00:00:00

  • Characterisation of benzimidazole binding with recombinant tubulin from Giardia duodenalis, Encephalitozoon intestinalis, and Cryptosporidium parvum.

    abstract::The binding kinetics of several benzimidazole compounds were determined with recombinant tubulin from benzimidazole-sensitive and -insensitive organisms. This study utilised the naturally occurring high efficacy of the benzimidazoles for the parasitic protozoa Giardia duodenalis and Encephalitozoon intestinalis, and l...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: MacDonald LM,Armson A,Thompson AR,Reynoldson JA

    更新日期:2004-11-01 00:00:00

  • Mosquito infectivity is directly related to the proportion of repetitive DNA in Plasmodium berghei.

    abstract::A strain of Plasmodium berghei (NK 65) was followed during syringe transmission in mice for over 120 passages after the last complete cycle, while the following parameters were monitored: (a) capacity to infect mosquitoes, inducing oocyst formation; (b) presence in the peripheral blood of morphologically identifiable ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Birago C,Bucci A,Dore E,Frontali C,Zenobi P

    更新日期:1982-07-01 00:00:00

  • Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation.

    abstract::Apicomplexan parasites utilize a peripheral membrane system called the inner membrane complex (IMC) to facilitate host cell invasion and parasite replication. We recently identified a novel family of Toxoplasma IMC Sub-compartment Proteins (ISP1/2/3) that localize to sub-domains of the IMC using a targeting mechanism ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fung C,Beck JR,Robertson SD,Gubbels MJ,Bradley PJ

    更新日期:2012-08-01 00:00:00

  • Inhibitory and neutral antibodies to Plasmodium falciparum MSP119 form ring structures with their antigen.

    abstract::Blood-stage malaria vaccine candidates include surface proteins of the merozoite. Antibodies to these proteins may either block essential steps during invasion or render the merozoite susceptible to phagocytosis or complement-mediated degradation. Structural information on merozoite surface proteins complexed to antib...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dekker C,Uthaipibull C,Calder LJ,Lock M,Grainger M,Morgan WD,Dodson GG,Holder AA

    更新日期:2004-09-01 00:00:00

  • Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.

    abstract::The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the struct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chen M,Li E,Stanley SL Jr

    更新日期:2004-10-01 00:00:00