Identification of the Echinococcus (hydatid disease) organisms using cloned DNA markers.

abstract：:The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

journal_title：Molecular and biochemical parasitology

authors： Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

更新日期：1990-12-01 00:00:00

abstract：:Apicomplexa parasites use complex cell cycles to replicate that are not well understood mechanistically. We have established a robust forward genetic strategy to identify the essential components of parasite cell division. Here we describe a novel temperature sensitive Toxoplasma strain, mutant 13-20C2, which growth a...

journal_title：Molecular and biochemical parasitology

authors： Suvorova ES,Lehmann MM,Kratzer S,White MW

更新日期：2012-01-01 00:00:00

abstract：:Schistosoma mansoni leucine aminopeptidase (LAP) is thought to play a central role in hatching of the miracidium from the schistosome egg. We identified two discrete LAPs genes in the S. mansoni genome, and their orthologs in S. japonicum. The similarities in sequence and exon/intron structure of the two genes, LAP1 a...

journal_title：Molecular and biochemical parasitology

authors： Rinaldi G,Morales ME,Alrefaei YN,Cancela M,Castillo E,Dalton JP,Tort JF,Brindley PJ

更新日期：2009-10-01 00:00:00

abstract：:We have demonstrated that bradyzoites return to the tissue-cyst stage by a developmental pathway that is indistinguishable from that initiated by sporozoites. Mature bradyzoites, like sporozoites from oocysts, were non-proliferative as they contained uniform 1N DNA contents, and replication occurred only in parasites ...

journal_title：Molecular and biochemical parasitology

authors： Radke JR,Guerini MN,Jerome M,White MW

更新日期：2003-10-01 00:00:00

abstract：:We have previously provided evidence for a pathway in Plasmodium falciparum-infected erythrocytes, coined the parasitophorous duct pathway, which provides serum (macro)molecules direct access to intraerythrocytic parasites . The present study addresses the purity of the fluorescent macromolecules used to define the du...

journal_title：Molecular and biochemical parasitology

authors： Goodyer ID,Pouvelle B,Schneider TG,Trelka DP,Taraschi TF

更新日期：1997-07-01 00:00:00

abstract：:We have cloned and sequenced a homologue of the ring-infected erythrocyte surface antigen (RESA) gene from Plasmodium falciparum designated RESA-2. Two reading frames with high homology to exon 1 and exon 2 of RESA at both the nucleotide and amino acid levels were identified in the RESA-2 sequence. However, RESA-2 doe...

journal_title：Molecular and biochemical parasitology

authors： Cappai R,Kaslow DC,Peterson MG,Cowman AF,Anders RF,Kemp DJ

更新日期：1992-09-01 00:00:00

abstract：:In trypanosomes, trans-splicing is a major essential RNA-processing mechanism that involves the addition of a spliced leader sequence to all mRNAs from a small RNA species, known as the spliced leader RNA (SL RNA). SL RNA maturation is poorly understood and it is not clear where assembly with Sm proteins takes place. ...

journal_title：Molecular and biochemical parasitology

authors： Biton M,Mandelboim M,Arvatz G,Michaeli S

更新日期：2006-12-01 00:00:00

abstract：:Adult males and females of the dog heartworm, Dirofilaria immitis, were extracted separately and, following separation of the free and conjugated ecdysteroid fractions, the conjugates were hydrolysed enzymically. Both the ecdysteroids released by hydrolysis of the conjugates and the free hormones were further purified...

journal_title：Molecular and biochemical parasitology

authors： Mendis AH,Rose ME,Rees HH,Goodwin TW

更新日期：1983-11-01 00:00:00

abstract：:To further analyze the organization of the nuclear rDNA locus in Trypanosoma cruzi, genomic recombinant plasmid clones were constructed and isolated after hybridization with rRNA molecules as hybridization probes. Approximately 11 kilobase pairs from the cistron were cloned in three recombinant plasmids carrying adjac...

journal_title：Molecular and biochemical parasitology

authors： Hernández R,Díaz-de Léon F,Castañeda M

更新日期：1988-01-15 00:00:00

abstract：:Cryptosporidium spp. are intracellular apicomplexan parasites that cause outbreaks of waterborne diarrheal disease worldwide. Previous studies had identified a Cryptosporidium parvum sporozoite antigen, CpMuc4, that appeared to be involved in attachment and invasion of the parasite into intestinal epithelial cells. Cp...

journal_title：Molecular and biochemical parasitology

authors： Paluszynski J,Monahan Z,Williams M,Lai O,Morris C,Burns P,O'Connor R

更新日期：2014-02-01 00:00:00

abstract：:It has been shown that vaccination with two low molecular mass excretory secretory (ES) antigens of 15 and 24 kDa, respectively, afforded a substantial degree of protection against Haemonchus contortus to sheep. In vitro cultivation of the parasite usually yields a limited amount of these proteins and therefore, recom...

journal_title：Molecular and biochemical parasitology

authors： Schallig HD,van Leeuwen MA,Verstrepen BE,Cornelissen AW

更新日期：1997-09-01 00:00:00

abstract：:Sulfated glycosphingolipids are present on the surface of a variety of cells. They are active participants in adhesion processes in many systems and appear to be involved in the regulation of cell proliferation, differentiation and other developmental cellular events. However, the body of knowledge about synthesis, st...

journal_title：Molecular and biochemical parasitology

authors： Landoni M,Duschak VG,Peres VJ,Nonami H,Erra-Balsells R,Katzin AM,Couto AS

更新日期：2007-07-01 00:00:00

abstract：:The synchronization of intraerythrocytic maturation of Plasmodium parasites is an important factor in the malaria infection process. Synchronization is mediated by inositol phosphate (InsP(x))-induced Ca(2+)-release from internal stores. To further investigate the InsP(x) metabolism in these parasites a Plasmodium pro...

journal_title：Molecular and biochemical parasitology

authors： Stritzke C,Nalaskowski MM,Fanick W,Lin H,Mayr GW

更新日期：2012-12-01 00:00:00

abstract：:Trichomonas vaginalis is a parasitic protozoan of both medical and biological relevance. Transcriptional studies in this organism have focused mainly on type II pol promoters, whereas the elements necessary for transcription by polI or polIII have not been investigated. Here, with the aid of a transient transcription ...

journal_title：Molecular and biochemical parasitology

authors： Franco B,Hernández R,López-Villaseñor I

更新日期：2012-09-01 00:00:00

abstract：:Cytoadherence of infected erythrocytes is a hallmark of Plasmodium falciparum infection and a key determinant in the particular virulence of this species. Infected erythrocytes bind a variety of host receptors but certain adhesion traits are associated with more severe disease. A large, diverse protein family named P....

journal_title：Molecular and biochemical parasitology

authors： Kraemer SM,Gupta L,Smith JD

更新日期：2003-06-01 00:00:00

abstract：:Whilst the schistosome tegument has been intensively studied there is little information about processes in the gut, the other major interface with the bloodstream, apart from the well characterised cascade of proteases involved in haemoglobin digestion. To gain insights into gut function we undertook a proteomic anal...

journal_title：Molecular and biochemical parasitology

authors： Hall SL,Braschi S,Truscott M,Mathieson W,Cesari IM,Wilson RA

更新日期：2011-09-01 00:00:00

abstract：:The interaction of Leishmania promastigote surface protease (PSP) with the plasmatic protease inhibitor alpha 2-macroglobulin (alpha 2M) was investigated. In plasma, solubilized PSP forms covalent complexes only with alpha 2M, at the exclusion of other protease inhibitors. The formation of complexes is accompanied by ...

journal_title：Molecular and biochemical parasitology

authors： Heumann D,Burger D,Vischer T,de Colmenares M,Bouvier J,Bordier C

更新日期：1989-02-01 00:00:00

abstract：:The apicoplast is a highly specialized organelle that mediates required functions in the growth and replication of apicomplexan parasites. Despite structural conservation of the apicoplast among different parasite genera and species, there are also critical differences in the metabolic requirements of different parasi...

journal_title：Molecular and biochemical parasitology

authors： Caballero MC,Pedroni MJ,Palmer GH,Suarez CE,Davitt C,Lau AO

更新日期：2012-02-01 00:00:00

abstract：:In Trypanosoma brucei, the active variant surface glycoprotein genes (vsg) are located at telomeric expression sites (ES), whose expression is highly regulated during the life cycle. In the procyclic form, all ESs are repressed. In the bloodstream form, where antigenic variation occurs, only one of approximately 20 ES...

journal_title：Molecular and biochemical parasitology

authors： Navarro M,Cross GA

更新日期：1998-07-01 00:00:00

abstract：:An expression vector was constructed to express foreign genes in Trypanosoma congolense. The foreign gene and a neomycin phosphotransferase (NPT) gene are flanked by glutamate and alanine rich protein (GARP) gene processing signals and their expression is driven by a ribosomal RNA gene promoter. The plasmid is not mai...

journal_title：Molecular and biochemical parasitology

authors： Downey N,Donelson JE

更新日期：1999-10-25 00:00:00

abstract：:The major surface antigen (30 kDa) of Brugia pahangi has been characterised by a number of biochemical and immunochemical means. The 30 kDa polypeptide is a glycoprotein which can be extracted from the worm surface by homogenization in the absence of detergents. The 30 kDa polypeptide can be metabolically labelled wit...

journal_title：Molecular and biochemical parasitology

authors： Devaney E

更新日期：1988-01-01 00:00:00

abstract：:The sequence of two fragments derived from the variable region of the kinetoplast maxicircle of Trypanosoma brucei has been determined. One fragment (1334 nucleotides, situated immediately upstream of the 12S and 9S ribosomal RNA genes) consists of non-repetitious DNA, which does not hybridize to other maxicircle regi...

journal_title：Molecular and biochemical parasitology

authors： de Vries BF,Mulder E,Brakenhoff JP,Sloof P,Benne R

更新日期：1988-01-01 00:00:00

abstract：:Kinetoplast DNA (kDNA), the mitochondrial DNA of trypanosomes, is a network of thousands of topologically interlocked DNA minicircles and about 50 maxicircles. In this study, we have analysed the kDNA molecules of 6 strains of Trypanosoma evansi from different geographical areas. 2 strains were found to be dyskinetopl...

journal_title：Molecular and biochemical parasitology

authors： Ou YC,Giroud C,Baltz T

更新日期：1991-05-01 00:00:00

abstract：:Theileria annulata is an important pathogen of cattle in the tropics. The gene sequence of a sporozoite surface antigen (SPAG-1) is reported. Data is also presented demonstrating that SPAG-1 is synthesised as a large precursor. This antigen, which is a candidate for inclusion in a subunit vaccine, shows a remarkable d...

journal_title：Molecular and biochemical parasitology

authors： Hall R,Hunt PD,Carrington M,Simmons D,Williamson S,Mecham RP,Tait A

更新日期：1992-07-01 00:00:00

abstract：:Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/P...

journal_title：Molecular and biochemical parasitology

authors： Garcia-Silva MR,Frugier M,Tosar JP,Correa-Dominguez A,Ronalte-Alves L,Parodi-Talice A,Rovira C,Robello C,Goldenberg S,Cayota A

更新日期：2010-06-01 00:00:00

abstract：:Plasmodium falciparum malaria parasites invade human red blood cells and immediately begin making significant alterations to the structure of the erythrocyte. These alterations facilitate the movement of nutrients into, and waste products and parasite-derived proteins out of the cell to meet the needs of the growing p...

journal_title：Molecular and biochemical parasitology

authors： Deitsch KW,Wellems TE

更新日期：1996-02-01 00:00:00

abstract：:Trichuris arvicolae and T. muris are gastro-intestinal nematodes of respectively arvicoline and murine rodents. We aim to investigate the ecology of these Trichuris species using population genetics. We sampled nematodes from rodents trapped in the East of France. After confirming the species identification of the nem...

journal_title：Molecular and biochemical parasitology

authors： Deter J,Galan M,Charbonnel N,Cosson JF

更新日期：2009-10-01 00:00:00

abstract：:A Trypanosoma cruzi genomic expression library was screened with a pool of sera obtained from chronic chagasic patients. The recombinant antigen (Tc40) isolated from this library reacted with a large number of serum samples of chronic chagasic patients, suggesting that the presence of anti-Tc40 antibodies may be speci...

journal_title：Molecular and biochemical parasitology

authors： Lesénéchal M,Duret L,Cano MI,Mortara RA,Jolivet M,Camargo ME,da Silveira JF,Paranhos-Baccalà G

更新日期：1997-08-01 00:00:00

abstract：:The phosphoinositide phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) plays crucial roles in the maintenance of lysosome/vacuole morphology, membrane trafficking and regulation of endolysosome-localized membrane channel activity. In Toxoplasma gondii, we previously reported that PI(3,5)P2 is essential for parasite su...

journal_title：Molecular and biochemical parasitology

authors： Daher W,Morlon-Guyot J,Alayi TD,Tomavo S,Wengelnik K,Lebrun M

更新日期：2016-05-01 00:00:00

abstract：:Seven beta-naphthylamine-linked peptides were tested as substrates for a previously described thiol proteinase of adult Schistosoma mansoni. The enzyme was not active on carbobenoxy-arginyl-arginyl-4-methoxy-2-naphthylamide and carbobenzoxy-alanyl-arginyl-arginyl-4-methoxy-2-naphthylamide. Enzyme activity was maximal ...

journal_title：Molecular and biochemical parasitology

authors： Dresden MH,Rutledge ML,Chappell CL

更新日期：1981-11-01 00:00:00