A developmentally regulated cysteine protease gene family in Haemonchus contortus.

Abstract:

:The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans 8 kb and appears to contain 11 introns, ranging in size from 57 bp to over 5.2 kb. One of the introns interrupts the proposed active site region that is conserved between the H. contortus protease and the related thiol proteases cathepsin B and papain. Southern blot hybridization experiments indicate that the protease is encoded by a small gene family in H. contortus. Rabbit antisera prepared against the recombinant protein react on Western blots with 35 and 37-kDa proteins of adult worms. These proteins were not detectable by Western blot analysis in three larval parasitic developmental stages of H. contortus. Northern blot hybridizations indicate that mRNA transcripts for the gene family are present at low levels in a mixed population of third- and fourth-stage larvae but highly abundant in adult worms. Expression of the protease correlates with blood-feeding and suggests a role for the protease in blood digestion.

journal_name

Mol Biochem Parasitol

authors

Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

doi

10.1016/0166-6851(90)90143-a

subject

Has Abstract

pub_date

1990-12-01 00:00:00

pages

181-91

issue

2

eissn

0166-6851

issn

1872-9428

pii

0166-6851(90)90143-A

journal_volume

43

pub_type

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