Characterization and detection of plant trypanosomatids by sequence analysis of the small subunit ribosomal RNA gene.

abstract：:L-myo-Inositol 1-phosphate synthase (I-1-P synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. Inositol is a precursor of essential macromolecules like membrane phospholipids, GPI anchor proteins and lipophos...

journal_title：Molecular and biochemical parasitology

authors： Lohia A,Hait NC,Majumder AL

更新日期：1999-01-05 00:00:00

abstract：:A screen for Leishmania mexicana genes encoding promastigote-specific flagellar proteins resulted in isolation of genes encoding the major components of the paraflagellar rod. One of these, PFR-2, was characterized extensively. PFR-2 genes are present in the genome as a tandem array of three genes designated PFR-2A, P...

journal_title：Molecular and biochemical parasitology

authors： Moore LL,Santrich C,LeBowitz JH

更新日期：1996-10-01 00:00:00

abstract：:Schistosomiasis remains a severe problem of public health in developing countries. Several reports show that praziquantel, the drug of choice for treating schistosomiasis, can select Schistosoma mansoni strains resistant to the drug. Thus, developing new drugs against this parasitosis is a highly desirable goal. Curcu...

journal_title：Molecular and biochemical parasitology

authors： Morais ER,Oliveira KC,Magalhães LG,Moreira EB,Verjovski-Almeida S,Rodrigues V

更新日期：2013-02-01 00:00:00

abstract：:The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

journal_title：Molecular and biochemical parasitology

authors： Thompson SN,Platzer EG,Lee RW

更新日期：1987-01-02 00:00:00

abstract：:A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secr...

journal_title：Molecular and biochemical parasitology

authors： Hines SA,Palmer GH,Brown WC,McElwain TF,Suarez CE,Vidotto O,Rice-Ficht AC

更新日期：1995-02-01 00:00:00

abstract：:The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential...

journal_title：Molecular and biochemical parasitology

authors： Haile S,Cristodero M,Clayton C,Estévez AM

更新日期：2007-01-01 00:00:00

abstract：:Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

journal_title：Molecular and biochemical parasitology

authors： Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

更新日期：2012-03-01 00:00:00

abstract：:Glucose is a major source of energy and carbon in promastigotes of Leishmania mexicana, and its uptake is mediated by three glucose transporters whose genes are encoded within a single cluster. A null mutant in which the glucose transporter gene cluster was deleted by homologous gene replacement was generated previous...

journal_title：Molecular and biochemical parasitology

authors： Rodriguez-Contreras D,Feng X,Keeney KM,Bouwer HG,Landfear SM

更新日期：2007-05-01 00:00:00

abstract：:The identification and characterization of a recombinant cDNA clone, designated OV9M, expressing an antigen present in Onchocerca volvulus infective larvae and adult stages is described. Clone OV9M was identified by screening a lambda gt11 cDNA expression library derived from adult O. volvulus mRNA using pooled rabbit...

journal_title：Molecular and biochemical parasitology

authors： Irvine M,Huima T,Prince AM,Lustigman S

更新日期：1994-05-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite of the intestinal epithelium that has caused numerous outbreaks of diarrheal illness in humans. During our studies of the host immune response to C. parvum infection, we noted that two of the immunodominant surface antigens of the sporozoite stage of the parasite readily ...

journal_title：Molecular and biochemical parasitology

authors： Priest JW,Xie LT,Arrowood MJ,Lammie PJ

更新日期：2001-03-01 00:00:00

abstract：:The binding kinetics of several benzimidazole compounds were determined with recombinant tubulin from benzimidazole-sensitive and -insensitive organisms. This study utilised the naturally occurring high efficacy of the benzimidazoles for the parasitic protozoa Giardia duodenalis and Encephalitozoon intestinalis, and l...

journal_title：Molecular and biochemical parasitology

authors： MacDonald LM,Armson A,Thompson AR,Reynoldson JA

更新日期：2004-11-01 00:00:00

abstract：:The Plasmodium falciparum Erythrocyte Binding Antigen-175, EBA-175, is a soluble merozoite stage parasite protein which binds to glycophorin A surface receptors on human erythrocytes. We have expressed two conserved cysteine-rich regions, region II and region VI, of this protein as soluble His-tagged polypeptides in i...

journal_title：Molecular and biochemical parasitology

authors： Ockenhouse CF,Barbosa A,Blackall DP,Murphy CI,Kashala O,Dutta S,Lanar DE,Daugherty JR

更新日期：2001-03-01 00:00:00

abstract：:Nearly all mitochondrial RNA polymerase genes identified to date are encoded in the nucleus and have similarities to T3 and T7 bacteriophage RNA polymerases. Some chloroplast genes are also transcribed by T3/T7 phage-like RNA polymerases, raising the possibility that the apicomplexan parasites, which have both a mitoc...

journal_title：Molecular and biochemical parasitology

authors： Li J,Maga JA,Cermakian N,Cedergren R,Feagin JE

更新日期：2001-04-06 00:00:00

abstract：:The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

journal_title：Molecular and biochemical parasitology

authors： Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

更新日期：2010-10-01 00:00:00

abstract：:The livers of chickens maintained on a diet containing an effective in vivo level (70 ppm) of arprinocid, an anticoccidial agent, were found to contain 0.64 ppm of this drug and 0.33 ppm of a metabolite, arprinocid-1-N-oxide. When arprinocid was tested against Eimeria tenella in cultures of chick kidney epithelial cel...

journal_title：Molecular and biochemical parasitology

authors： Wang CC,Simashkevich PM

更新日期：1980-10-01 00:00:00

abstract：:The Plasmodium MSP-1 is a promising malaria vaccine candidate. However, the highly polymorphic nature of the MSP-1 gene (msp1) presents a potential obstacle for effective vaccine development. To investigate the evolutionary history of msp1 polymorphism in P. vivax, we construct phylogenetic trees of msp1 from P. vivax...

journal_title：Molecular and biochemical parasitology

authors： Tanabe K,Escalante A,Sakihama N,Honda M,Arisue N,Horii T,Culleton R,Hayakawa T,Hashimoto T,Longacre S,Pathirana S,Handunnetti S,Kishino H

更新日期：2007-11-01 00:00:00

abstract：:Studies were made of the effects of diamphenethide-amine on glucose transport, glycogen breakdown, adenine nucleotides, metabolites and excretory products in both parenchymal and bile duct flukes in vitro and in bile duct flukes in vivo. The most consistent and pronounced effect observed was an elevation of malate lev...

journal_title：Molecular and biochemical parasitology

authors： Edwards SR,Campbell AJ,Sheers M,Moore RJ,Montague PE

更新日期：1981-04-01 00:00:00

abstract：:Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These clone...

journal_title：Molecular and biochemical parasitology

authors： Campos MC,Leon LL,Taylor MC,Kelly JM

更新日期：2014-01-01 00:00:00

abstract：:Surface membrane proteins of viable merozoites of Plasmodium chabaudi were iodinated by the Iodogen method and analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirteen surface membrane proteins ranging from 22 to 270 kDa were thus identified. Most of these proteins could be immunoprecipitated by...

journal_title：Molecular and biochemical parasitology

authors： Franco da Silveira J,Falanga PB,Pereira da Silva L

更新日期：1984-01-01 00:00:00

abstract：:Antibodies raised against a Leishmania major recombinant promastigote surface antigen 2 (PSA-2) fragment recognized three major polypeptides of approximate M(r) 96,000, 80,000 and 50,000 in promastigotes of three Israeli isolates of L. major including the cloned line LRC-L137-V121, but detected a different array of po...

journal_title：Molecular and biochemical parasitology

authors： Symons FM,Murray PJ,Ji H,Simpson RJ,Osborn AH,Cappai R,Handman E

更新日期：1994-09-01 00:00:00

abstract：:The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

journal_title：Molecular and biochemical parasitology

authors： Cunha VM,Meyer-Fernandes JR,Noël F

更新日期：1992-06-01 00:00:00

abstract：:Post-transcriptional regulation is a key feature controlling gene expression in the protozoan parasite Leishmania. The nine-nucleotide paraflagellar rod regulatory element (PRE) in the 3'UTR of Leishmania mexicana PFR2 is both necessary and sufficient for the observed 10-fold higher level of PFR2 mRNA in promastigotes...

journal_title：Molecular and biochemical parasitology

authors： Holzer TR,Mishra KK,LeBowitz JH,Forney JD

更新日期：2008-01-01 00:00:00

abstract：:The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

journal_title：Molecular and biochemical parasitology

authors： MacLeod A,Turner CM,Tait A

更新日期：1999-08-20 00:00:00

abstract：:In Trypanosoma brucei, the active variant surface glycoprotein genes (vsg) are located at telomeric expression sites (ES), whose expression is highly regulated during the life cycle. In the procyclic form, all ESs are repressed. In the bloodstream form, where antigenic variation occurs, only one of approximately 20 ES...

journal_title：Molecular and biochemical parasitology

authors： Navarro M,Cross GA

更新日期：1998-07-01 00:00:00

abstract：:Trypanosoma brucei survives in mammals by antigenic variation of its surface coat consisting of variant surface glycoprotein (VSG). Trypanosomes change coat mainly by replacing the transcribed VSG genes in an active telomeric expression site by a different VSG gene. There are about 20 different expression sites and tr...

journal_title：Molecular and biochemical parasitology

authors： Borst P,Ulbert S

更新日期：2001-04-25 00:00:00

abstract：:ATP-binding cassette transporters (ABC transporters) are essential components of normal cellular physiological machinery in all eukaryotic and prokaryotic species, including parasites. Some ABC transporters, e.g., P-glycoproteins, are involved in the efflux of toxins and xenobiotics from the cell. At present, nothing ...

journal_title：Molecular and biochemical parasitology

authors： Mordvinov VA,Ershov NI,Pirozhkova DS,Pakharukov YV,Pakharukova MY

更新日期：2017-09-01 00:00:00

abstract：:The complex molecular systems involved in the process of sex-differentiation and fertility in Schistosoma mansoni have not yet been completely described. Using a 4608-element cDNA microarray, we have now determined 90 and 139 genes with significantly (q-value

journal_title：Molecular and biochemical parasitology

authors： DeMarco R,Oliveira KC,Venancio TM,Verjovski-Almeida S

更新日期：2006-12-01 00:00:00

abstract：:Cloning by limit dilution of an isolate of Leishmania tropica (LRC-L137) that is infective for mice resulted in 7 stable clones, only one of which was infective in BALB/c mice. Three of the non-infective clones that were examined for survival in BALB/c macrophages in vitro seemed to be killed more readily, suggesting ...

journal_title：Molecular and biochemical parasitology

authors： Handman E,Hocking RE,Mitchell GF,Spithill TW

更新日期：1983-02-01 00:00:00

abstract：:GRA3 is a 30-kDa protein located inside the dense granules of Toxoplasma gondii. Following invasion and exocytosis of dense granules within the parasitophorous vacuole, GRA3 becomes associated with the parasitophorous vacuolar membrane (PVM) and extensions of the PVM which protrude into the cytoplasm. A partial cDNA e...

journal_title：Molecular and biochemical parasitology

authors： Bermudes D,Dubremetz JF,Achbarou A,Joiner KA

更新日期：1994-12-01 00:00:00

abstract：:CK2 is a ubiquitous but enigmatic kinase. The difficulty in assigning a role to CK2 centers on the fact that, to date, no biologically relevant modulator of its function has been identified. One common theme revolves around a constellation of known substrates involved in growth control, compatible with its concentrati...

journal_title：Molecular and biochemical parasitology

authors： Jensen BC,Kifer CT,Brekken DL,Randall AC,Wang Q,Drees BL,Parsons M

更新日期：2007-01-01 00:00:00